DAB
70 1 S-100 2 3 EMA 4 Ig κλ B B Ig κ+/λ+ Ig κ+λ+ bcl-2 90% bcl-2 bcl-2 5 6
7 8 c-erb-2cmycp53 nm23 EGFR PCNAKi-67 Ki-67 PCNA EGFR 9 10 6 T B 10
CMVHPVHSV HBV 1 1.1 5~10 1.2 1~2ml HankRPMI-1640 500r/min 5~10min110 6 /ml 1 1.3 1500r/min 10min 1.4 1.5
210 5~6 /ml 50100l1000r/min 2min 0.6~1cm 2 10 5 l2h 24
Poly-L-Lysine () 30000 0.5% 110 60 2.5g 500ml 0.25g 2 min APES (3--) 1:50 APES 20s APES
PH Sigma Santa Cruz 10mg/ml 0.1%-1.5% 0.01-0.15NaN 3 NaN 3-20 20 28 4 1-3 7 10 2 28 2020