NLAC 主題專欄 RMRC RMRC A. B. C. A. B.

Vol.3 April 2009 國家實驗動物中心簡訊 National Laboratory Animal Center Newsletter 2 4 00 00 5 7 15 (Rodent Model Resource Center, RMRC) RMRC RMRC 00 RMRC (00) 1 1RMRC 19 0 Animal Hotel 21 22 23

NLAC 主題專欄 RMRC RMRC A. B. C. http://www.nlac.org.tw/rmrc A. B.

Vol.3 April 2009 National Laboratory Animal Center Newsletter C. D. 1 1 1 1 0 0(/1) / 11% 00/0/01 0-1 0- e-mail geniechin@nlac.arg.tw 0- e-mail joyce@nlac.arg.tw 0-0 0- e-mail domeco@nlac.org.tw 0- e-mail biojackson@nlac.org.tw 2008 1 11 11 1 11 1.00 1 /.1010 RIKEN BRC http://www.nlac.org.tw/products-list.aspx h t t p : / / w w w. n l a c. o r g. t w / d m o u s e / c h / p a g e / 0_serviceszone/0_serviceszone.html http://www.nlac.org.tw/complant/boardpost.aspx 10 1 http://www. nlac.org.tw/index.aspx 10 00 11CBL/JNarl 1 1.00PCR.00

NLAC 最新動態 2009 1.. A. (1) ELISA H1 ECUN Reo EDIM ECUN 00 () IFA1 00 1 B.. 00 1.00 1 10. A. 00 1 B. 00 1 (1) -hemolytic Streptococcus 00 1 ()Klebsiella oxytoca 00 1. 00 1. A. 00 1 B.00 C. 00 D. IVIS 00 E. (1)CBL/JNarl 00 11 () BALB/cAnN.Cg-Foxn1 nu /CrlNarl 00 1 ()BALB/cByJNarl 00. 00 1. 00 10.

Vol.3 April 2009 National Laboratory Animal Center Newsletter 2008!! 00 00 11 10 11 1 USER 00 1 00 USER FQ&A Q&A www.nlac.org.tw strawnlac protocol RIKENknockout mice RIKEN QA/QC genotyping 1..

NLAC 年度活動 00 00 00 00 10 11 10

Vol.3 April 2009 National Laboratory Animal Center Newsletter (RIKEN BRC) 0 / BRC BRC BRCLCMV LCMV IFA RT-PCR RIKEN

NLAC 國際交流 (Asian Mouse Mutagenesis Resource Association, AMMRA) 00 NIHThe Knockout Mouse Project (KOMP) AMMRA The International Gene Trap Consortium (IGTC)The European Conditional Mouse Mutagenesis Program (EUCOMM) AMMRA (Asian Federation of Laboratory Animal Science Association, AFLAS) 00 00 ICLAS010AFLAS AFLASDr. Kasai

Vol.3 April 2009 National Laboratory Animal Center Newsletter ( T h e Jackson Laboratory)00 11 Colony Management: Principles and Practice (Mouse genome informatics; MGI)1 (genotype) (phenotype) Dr. John Macauley 1.(http://www.informatics.jax.org/)

NLAC 國際交流 2008 (International Mammalian Genome Society, IMGC) IMGC 00 11 Praha Doc 00~0 RIKEN 1The Jackson Laboratory 1 Mammalian Genomes, Epigenomes, Phenotypes, Bioinformatics. Epigenomes (1)Comparative Genomics / Computational Biology. ()Development / Cancer / Biology / Aging. ()Epigenetics, Immunity / Infection. ()Modeling Disease. ()Mutagenesis and Neuroscience / Behaviour. Cornell UniversityPost- 10

Vol.3 April 2009 National Laboratory Animal Center Newsletter 2008 (American Association of Laboratory Animal Science, AALAS) (Animal Welfare) 00 111 Convention Center PI Tech Week: Laboratory animal technicians - Heroes behind the science 00 11

NLAC 國際交流 (Transgenic Technology Symposia 00,TT00) (International Society for Transgenic Technologies, ISTT) Transgenic Research Dr. Andras Nagy 2008 00(Super Computing 0 SC0) Austin,Texas,USA11 1 1 HPC Intel IBM nvidia 00/11/1 11/1Live DemoD Digital Mouse Project D VR IT D SC HPC GPU VR DD NEXSANTB K10 Stanford Visible Human Project Dataset segmentation D SC0 12

Vol.3 April 2009 National Laboratory Animal Center Newsletter 00 Establish Resource/Service Center for Mutant Mice and Disease Models in China One Gene, Multiple Target: Fstl1 Gene Targeting 13

NLAC 國際交流 2007Dr. Oliver Smithies 00Dr. Oliver Smithies Dr. Nobuyo Maeda 00 10 Dr. Britton Chance 14

Vol.3 April 2009 National Laboratory Animal Center Newsletter -MFIAELISA MFIA(Multiplexed Flurometric ImmunoAssay) MFIAELISA ELISA Slide microarray coating beads (MFIA) ex. Charles River Laboratory(CRL) ELISA IFA side-by-side MFIA ELISA.%dataMFIA ELISA IFAIFACRLMFIA ELISA CRL 00 MFIA ELISA MFIA + - ELISA + # 1 %.% 0.% - # 0 % 0.%.% MFIA ELISA.% data (# of Assay:) Bead. m100 beadcoatingdual laser flurometer bead bead Biotin MFIA ELISA ELISA coating well (tissue control) MFIAtissue controlinternal control: Ig control beads Anti-Ig control beads Ig control beads Anti-Ig control beads Positive control Positive control System suitability control BSA or SPE Sample suitability control 1.serum.serumoverdiluted. immunocompromised host wrong species 15

NLAC 技術專欄 ELISA MFIA NO NO -well plate 1 items 0 L L MFIA 0 0 virus item 1~1 item 1 1 10 0 ABTS10 1 10 0 BSA SPE 10 0 MFIA items 11 10 score 1 negative score 1 negative score score equivocal score equivocal score or higher positive score or higher positive virus CsCl Accuracy 1.HI control.lo control.negative (Mouse non-immune) 1.HI control.lo control.negative.internal control (a) Ig control (b) Anti-Ig control MFIAControl 1.washer machine 1.washer machine.reader machine.reader machine.serum pump 10.shaker ELISA NTD 1item NTDitem 16

Vol.3 April 2009 National Laboratory Animal Center Newsletter (Murine norovirus, MNV) RNA (Reverse transcription polymerase chain reaction, RT-PCR)Hsu et al.clin. DIAGN. LAB. IMMUNOL.(Vol.1, No.10) 1 bp 1bp 10bp (Theiler's mouse encephalomyelitis virus, TMEV) (Mouse hepatitis virus, MHV) RT-PCR (Enzyme-linked immunosobent assay, ELISA) MNV ELISA RT-PCR ELISA 1 ELISA RT-PCR.% 1 ELISA1110 1.% RT- PCR MNV (specific pathogen free, SPF) (Barrier) -SPFC.B1/Icr-Prkdcscid/Crl CBL/JNarl (Lactobacillus spp.)11 SPF SPF -SPF 17

NLAC 技術專欄 Pasteurella penumotropica (Pasteurella pneumotropica) (ELISA) P. pneumotropica P. multocida Mannheimia haemolytica 1Pasteruella (Polymerase Chain Reaction, PCR) P.pneumotropica 1S r RNAMn-dependent superoxide dimutase(mn-sod) P. pneumotropica P. pneumotropica genomic DNA1ng 10fg germ-free 0ng genomic DNA1ng DNA P. pneumotropica Hamster Tyzzer's disease Clostridium piliforme Tyzzer's disease (spore) bedding Tyzzer s disease athymic T-cell deficient nude mice SCID mice ELISA cystatin C(CysC) CysC CysC tripeptide A CysC+/H* ex vivo Clostridium sp. Jackson LabC. piliforme genomic DNAtemplateprimer pairs(rj1/rj, 1S F/1S10R) positive control vector Clostridium piliforme PCR in vivo CysC+/H* A CysC A CysC A 18

Vol.3 April 2009 National Laboratory Animal Center Newsletter.. 000- :0-1:0 0 http://www.nlac.org. tw/workshop/main.htm 0-- Email biojackson@gmail.com Johns Hopkins University, School of Medicine, Baltimore, Rodent Phenotyping Core 1.... 19

NLAC 活動預告 50 Animal Hotel 0 Animal Hotel Animal Hotel 50 10 10 110 1 0http://www.nsc.gov.tw/0th/blogmp.asp?mp=11 TEL 0-1010 E-mail ming1@nlac.org.tw 10 11 1 0 20

Vol.3 April 2009 National Laboratory Animal Center Newsletter 00 00000 00001 00 00 (1) Animal Hotel () 1 () R 111 00 10 1 () IVIS 00 21

NLAC 用戶資訊 2008 Q1 A 00 00 Q2 A USER0 1100 or Q3 A SPF Q5 A Q6 knockout mice A 00 Q4 A http://www.nlac.org.tw/board-content. aspx? 22

Vol.3 April 2009 National Laboratory Animal Center Newsletter 2008 2008 12 25 00 1 u n p l u g 2008 11 10 LOHAS!! Go Go Go!!!!! 23

1......... 10. 0-1- 0--0 0-- 0-- 0-1-00# 0-10-00#110 0-10-00#10 0-- 0-- 0--0 128 02-2789-5506 02-2789-5533 http://www.nlac.org.tw