1. 云南烟草科学研究院, 云南昆明 650106; 2. 昆明理工大学化学工程学院, 云南昆明 650093; 3. 中国科学院西双版纳热带植物园, 云南昆明 650223 1 1 2 韩熠张承明喻坤 3 1 1 孟霞陈永宽缪明明 韩熠 (1976), 男, 博士, 助理研究员, 主要从事烟用材料有害成分分析方法研究 E-mail:hanyi762002@yahoo.com.cn 摘 要,, RecentAdvancesinAnalyticalMethodsforCoumarinsandSafrolesinFoodandCosmetics HAN Yi 1 ZHANG Chengming 1 YU Kun 2 MENG Xia 3 CHEN Yongkuan 1 MIAO Mingming 1 (1.YunnanAcademyofTobaccoScience,Kunming650106,Yunnan,China;2.ColegeofChemicalEngineering, KunmingUniversityofScienceandTechnology,Kunming650093,Yunnan,China;3.XishuangbannaTropicalBo- tanicalgarden,chineseacademyofsciences,kunming650223,yunnan,china) Abstract: Recentadvancesinanalyticalmethodsforcoumarinsandsafrolesinfoodandcosmeticsarereviewed.Diferent samplepreparationandanalyticalmethodsaredescribed.inaddition,themainproblemsandkeypointsoffuturedevelopment withpersonalperspectivesarediscussed. Key words: coumarin safrole gaschromatography liquidchromatography,,, [1], 10 mg/kg [2],, (EFSA), (TDI) 0.1mg/kg [3], 5 mg/, kg 50 mg/kg [4], (FoodandDrugAdmin- (SCCNFP) 2000 istration,fda) 1954 7-6-,FDA [6] 7-,, Regulation(EC)No1223/2009 :2012-08-19 [5] 7-7- 4- -7-45
[7], GB2760-2011, 40, 6- [8], 2010 7, 0.015mg/L, 2~10mg/L,,,,, 2.0mg/kg [9] GB7916-1987, 6-0.003% [10], LC-MS/MS (2007 ) [15] 7-7- LC-MS/MS 7- -4-, (ESI) 6 FDA,,, [16] 0.04%~1%,150d 2a [1] (InternationalFragranceAssociation),,,,, 0.01%, 6-7- -4- Raters [17] LC-MS/MS [11] 88/388/EEC 500 1 mg/kg, (d 4- ) 25% 5mg/kg, - (HPLC-UV), 15 mg/kg [12] 2005 [13] HPLC-UV 100 LC-MS/MS, Rychlik [18] LC-MS/MS [ 13 C 2 ]-, [ 13 C 2 ]-, (HPLC) - (NMR) (LC-MS/MS) (GC) - (GC-MS),, Vierikova [19] - (UPLC-ESI-MS/MS) 1 1.1 (HPLC) - (ASE), (GPC) (LC-MS/MS), LC-MS/MS,, LC-MS/MS 0.03mg/kg 0.05mg/kg 2.9μg/kg 8.6μg/kg ASE UPLC [14] 1999, HPLC 20 μg/kg 275nm 50μg/kg 46
1.2 (GC), - 6 (7- (GC-FID) 7-7- 2001,Choong [20] GC-FID 278nm 308nm, 0.2~0.5mg/kg 50, 1,4- (DHB), 0.25μg/mL 25 20 [28] GC-FID HPLC-DAD 0.2μg/mL, -,, ph, 306 nm, 1ng GC-FID 2.2 (GC) - [22] ( ) (GC/MS) 2 Wisneski [29] GC-ECD( 2.1 (HPLC) - ) GC/MS, (LC-MS/MS) [21] 210nm 220nm 250nm 17, 1mg/kg 15, 1,4-, (DAD) [30] GC-FID 13, LC- 6- MS/MS [23] 2005, HPLC-DAD( HP-1, ) 6-, 6- [31] 275nm 1μg/g GC/MS, 15, 7-6- [24] HPLC-UV 6-3 : 0.3 0.5 0.3μg/mL 15 20min,, 275nm 0.05mg/kg 0.10mg/kg [25] [25] HPLC-DAD 10 [32] 3 ( 7-6- GC/MS ) 3 5 (7-0.03μg/mL 0.01μg/mL 7-7- -4-0.03μg/mL 14 16 ),, DB-1701 /, [26] HPLC-DAD -4- ), [27] HPLC-DAD 17 LC-MS/MS LC-MS/MS GC-FID GC/MS, DB-5MS, 47
, 5.0mg/kg,, 7-0.5mg/kg,7- -4-, 0.1mg/kg 45 ( UPLC) [33],,,,GC/MS 6-5μg/g 10μg/g 3,,GC-FID HPLC, fda.gov/.[2012-06-18].,, tp://db.foodmate.net.[2012-06-19]., GB28363-2012 [33] GB/T14454.1-2008 [34] ( ) GB/T 11538-2006 [35] (GC-FID ) GB/T14454.15-2008 [36],GC-FID GB/T24800.9-2009 [37], L354:34-50., GC-FID [38] 6-,GC-FID [39] GC/MS ;,HPLC-DAD 6- [40], HPLC-UV GC-FID, HPLC-UV ;,, 4,,HPLC LC- MS/MS GC-FID GC/MS tp://www.ifraorg.org.[2012-06-25]., 48 lations -Title21 -FoodandDrugs[EB/OL].htp://www. [3] EFSA.ScientificOpinionofthePanelonFood Additives, rininflavouringsandotherfoodingredientswithflavouring properties[j].theefsajournal,2008,793:1-15. [1] U.S.FoodandDrugAdministration.CodeofFederalRegu- [2] [EB/OL].ht- Flavourings,ProcessingAidsand MaterialsinContactwith FoodonarequestfromtheEuropeanCommissiononCouma- [4] EU Council.REGULATION (EC)No1334/2008OFTHE EUROPEANPARLIAMENT AND OF THE COUNCILof 16December2008onflavouringsandcertainfoodingredients withflavouringpropertiesforuseinandonfoodsandamen- dingcouncilregulation (EEC)No1601/91,Regulations (EC)No2232/96and (EC)No110/2008 and Directive 2000/13/EC[J].OficialJournalofthe European Union, [5] ParliamentoftheRepublicIndia.ThePreventionofFoodA- dulterationact& Rules,2004[EB/OL].htp://www.indi- aenvironmentportal.org.in/reports-documents/prevention- food-adulteration-act-rules-1102004.[2012-06-20]. [6] SCCNFP/0320/00,final.Aninitiallistofperfumerymateri- alswhichmustnotform partoffragrancescompoundsused incosmeticproducts.the12 th PlenarymeetingofSCCNFP, 3May2000[EB/OL].htp://ec.europa.eu/health/archive/ ph risk/.../out116 en.pdf.[2012-06-20]. [7] Consumersmaytakeinlargeamountsofcoumarinfromcos- metics,too[eb/ol].htp//www.bfr.bund.de/cd/10569. [2012-06-20]. [8].GB2760-2011 [S]. :,2011. [9]. [J].,2010(8):21. [10].GB7916-87 [S]. :,1987. [11] InternationalFragranceAssociation.IFRAStandard[EB/OL].ht- [12] TheCounciloftheEuropeanCommunities.88/388/EEC,
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