33 5 2017 5 Vol.33No.5 May.2017 DOI:10.13652/j.issn.1003-5788.2017.05.032 Themechanismforanti-drunkandanti-inebriationofKonjacGlucomannan 12 1 1 1 1 13 ZHENG Lian-ji 12 DENG Li-ling 1 LUO Jia-ni 1 ZHANG Shuai 1 DENG Li 1 (1. 4007162. 400042 ZHONG Geng 13 3. 400715) (1.Colegeof Food ScienceSouthwest UniversityChongqing400716China2.Chongqing Food TechnologyInstitute Chongqing400042China3.Chongqing Engineering Research Centerof RegionalFoodChongqing400715China) : 以不同剂量的魔芋葡甘露聚糖 (KonjacGlucomannan KGM) 灌胃昆明种小鼠 采用 56%vol 红星二锅头灌胃方法 进行造模 研究 KGM 对小鼠的抗醉解酒作用及机理结果 表明 : 中 (240mg/kg) 高 (400 mg/kg) 剂量组可有效延长醉 酒潜伏期 显著缩短醉酒小鼠的睡眠时间和醒酒时间 且高 中剂量组的血清乙醇浓度显著低于模型组小鼠 高剂量组小 鼠肝组织匀浆中乙醇脱氢酶 (Alcoholdehydrogenase ADH) 乙醛脱氢酶 (AcetaldehydedehydrogenaseALDH) 和细胞色素 P450(CytochromeP450P450) 含量均显著升高 中 高剂量组小鼠胃黏膜组织和血清中丙二醛 (Methanedi- carboxylicaldehydemda) 含量显著降低 过氧化物歧化酶 ideno) 含量 前列腺素 E2(ProstaglandinEPGE2) 含量显著升高其解酒机理 :1 可能是其抑制酒精的吸收 降低血 清中乙醇浓度 2 小鼠胃黏膜和血清中 MDA 含量的降低 SOD 活力 NO 和 PGE 的升高减轻了酒精对胃黏膜的损伤 并提高了肝脏中 ADH ALDH P450 含量 通过乙醇脱氢酶 和乙醇氧化酶系统加速酒精代谢 发挥其防醉解酒作用 : 魔芋葡甘露聚糖 抗醉解酒 作用机理 动物试验 Abstract:In this studydiferent doses of konjac gum powder (SuperoxideDismutaseSOD) 活力 一氧化氮 (NitricMonox- (KGM)wereadministeredtoKunmingmicetostudytheirtheanti- touchina.theresultsshowedthatthemiddledose (240 mg/kg) E-mail:zhongdg@126.com :2016 12 21 drunkhangoverefectsanditsmechanismandthemodelwasmade byintragastricadministrationof56 %volliquorofredstarerguo- andhighdose (400 mg/kg)ofkgm couldefectivelyprolongthe climbtimeandlatencytimeofdrunkennessandcouldsignificantly shortenthedrunkenmicesleepingtimeandsoberingtime.theetha- nolconcentrationofseruminthehighand middledosegroupswas significantlylowerthanthatinthemodelgroupandthecontentsof alcoholdehydrogenase (ADH)aldehydedehydrogenase (ALDH) creasedinhighdosegroup.thecontentsofmalondialdehyde(mda) andcytochromep450 (P450)inliverhomogenatesignificantlyin- ingastric mucosaltissuesandseruminthe middleandhighdose groupssignificantlydecreasedwhiletheactivitiesofsuperoxidedis- mutase(sod)nitricoxide(no)andprostaglandine2significantly increased.theanti-alcoholhangovermechanismofkgm mightbeas folows.ontheonehandalcoholabsorption wasinhibitedbythe KGM andtheethanolconcentrationinserum isreduced.onthe otherhandthedecreaseofmdacontentandtheincreaseofsod activityno andpgeingastric mucosaandserum decreasedthe damageofgastricmucosaandincreasedtheadhaldhp450. Thusandthealcoholmetabolism wasacceleratedthroughalcoholde- hydrogenase and ethanol oxidase system then its anti-drunk hangoverrolewasplayed. Keywords:KonjacGlucomannan(KGM)anti-drunk &anti-inebria- tionefect& mechanismanimalexperiment : ( :CSTC 2013jcyjA00028) 121 ( : [1] CSTC2014fazktjcsf80052) ( :CSTC2014pt-gc8001) : [2] [3] : (1964 ) [4] [5-6] [7] 156 KGM
2017 年第 5 期 [8] [9] KGM = - = - = KGM KGM 1.3 KGM 1 1 5( KGM 1.1 ) 10 1.1.1 12h KGM(160~200): >90% 0.4mL/20g 170240400 mg/kgkgm 30 min :56%vol KGM 56%vol 1.1.2 14mL/kg : 1.3.2 0.51.01.5 : 2.02.5h 37 (ADH) (ALDH) 2 h 3000~4000r/min (P450) (MDA) : 10min 4 2.5h (NO) (SOD) (PGE2) : [11] 1.1.3 1.3.3 KGM :5810 Eppendorf (1) : 50100150200 :HH-4 250300350 mg/100 ml ( 0.789~ :DHG-9070A 0.791g/mL)100μL 100 mg/100 ml ( 0.784~0.786g/mL)500μL3000r/min :JTI0001 5min 0.6μL :GC-2010 :HIMG ( ) ( ) 1.1.4 1 (20±2)g : SCXK-()2012-0011 : 22~24 40% ~60% 12h( 8:00~20:00) 1.2 KGM 1.2.1 40 1 4 10 12h 56%vol 13141516 ml/kg BW Figure1 Thestandardcurveofethanol 1.2.2 40 1 500 μl3000r/min 5 min [12] 4 10 4 12h 0.6μL 14mL/kg 56%vol (3) :GC-2010 :PEG-20M : ( ) :100mg/100mL 0.4 ml/20g 190 (FID) 240 170240400mg/kgKGM : 400 ml/min 40 ml/min ( ) - 1.3.1 50 3 [9-10] 55 6min 5 /min70 图 1 乙醇标准曲线 (2) : 100μL 0.45μm 40mL/min 0.97mL/min 0.6μL : 157
33 5 : 40s 30 /min 110 [13] 1.3.4 Table1 Theefectsofethanoldosageonmice SOD NO PGE2 TBA MDA ebriety(n=10) / / / / / / (ml kg -1 ) % % 1.3.5 13 10 6 0 60 0 14 10 9 1 90 10 ADH ALDH P450 15 10 9 2 90 20 16 10 10 4 100 40 1.4 SPSS18.0 2.2 KGM ± (LSD) 2.2.1 KGM P<0.05 2 120 min 2.1 KGM 90 min 2.1.1 KGM 120 min 1 13mL/kg 60% 0% 16 ml/kg (P>0.05) 100% KGM 40% 14 ml/kg 90% 10% 2.2.2 [10] (1) SOD MDA : [9] [14] 2.1.2 KGM 2 [15] MDA SOD (P>0.05) KGM [16] 1 3 KGM :6090150 min [1417] SOD SOD MDA 2 KGM Table2 Therapeuticefectofkonjacgumpowderonebrietyofmice(n 7) / / /min /min /min 9 1 26.09±15.35 b 315.08±75.61 a 336.52±50.05 a 10 0 23.98±8.40 b 284.37±48.02 ab 322.87±15.71 a 8 0 32.21±16.77 ab 225.17±59.31 bc 257.38±56.75 b 7 0 50.18±27.01 a 191.32±63.78 c 237.42±42.26 b 3 KGM Table3 Theefectsofkonjacgumpowderonethanolconcentrationoftimecoursesin seruminmice(n 8) mg/ml 30min 60min 90min 120min 150min 4.822±0.420 a 7.788±0.334 a 8.549±0.040 a 7.819±0.160 ab 7.675±0.133 a 4.865±0.152 a 7.769±0.366 a 8.044±0.107 a 8.048±0.167 a 7.652±0.248 a 4.622±1.078 a 5.726±0.299 b 6.143±0.940 b 7.714±0.170 ab 7.147±0.252 b 4.070±0.111 a 5.555±0.545 b 5.818±0.283 b 7.370±0.577 b 7.070±0.276 b 158
2017 年第 5 期 5 KGM MDA [18] KGM MDA Table5 Efectofkonjacgumpowderon MDAcontentand SOD 4 SODactivityin miceserum withalcohol-induced MDA SOD injury(n 8) MDA SOD MDA / SOD / (nmol ml -1 ) (U ml -1 Pro) MDA (P< 0.05) SOD SOD SOD 14.492±2.840 b 18.224±2.566 a 18.064±2.859 a 58.199±1.982 a 49.912±3.919 c 51.394±2.471 bc (P>0.05) 14.906±2.324 b SOD MDA 13.684±2.279 b 53.825±1.580 b 60.431±4.973 a (P>0.05) KGM MDA SOD KGM MDA SOD NO 4 KGM MDA 2 3 NO SOD Table4 Efectofkonjacgumpowderon MDAcontentand NO NO SODactivityin micegastric mucosawithalcohol- inducedinjury(n 8) MDA / (nmol mg -1 Pro) SOD / (U ml -1 Pro) 2.522±0.620 c 23.324±4.885 a 5.410±1.536 a 17.853±3.711 b 3.936±0.786 b 3.346±1.107 bc 2.849±1.033 bc 17.790±2.271 b 19.407±4.198 ab 23.629±5.305 a (4) PGE2 : (2)SOD MDA :KGM (PGs) MDA SOD [22] 5 SOD (P< PGE2 0.05) MDA MDA SOD MDA SOD MDA SOD SOD MDA (P>0.05) KGM MDA SOD NO NO KGM NO (P>0.05) NO (P>0.05) KGM KGM NO SOD 图 2 KGM 对酒精致胃黏膜损伤小鼠胃黏膜组织 NO (3) NO :NO 含量的影响 Figure2 Efectofkonjacgum powderon NO contentin micegastric mucosa withalcohol-inducedinjury [19-20] NO (n 8) 159
33 5 : PGE2 (P> [27] 0.05) KGM KGM PGE2 2.2.3 ADH ALDH P450 90% 图 3 KGM 对酒精致胃黏膜损伤小鼠血清 NO 含量的影响 (ADH) (ALDH) Figure3 Efectofkonjacgum powderon NO contentin ADH ALDH miceserum withalcohol-inducedinjury(n 8) [28] CO2H2O PGE2 10mmol/L (MEOS) P450 (NADPH) P450 [29] [23-25] [26] P450ⅡE1P450 [30] 4 5 PGE2 [30] P450ⅡE1 PGE2 ADH ALDH PGE2 P450 6 6 PGE2 ADH P450 PGE2 ADH PGE2 KGM ALDH P450 ADH ALDH P450 (P>0.05) KGM ADH ALDH P450 图 4 KGM 对酒精致胃黏膜损伤小鼠胃黏膜组织 PGE2 含量的影响 Figure4 Efectofkonjacgum powderonpge2contentin micegastric mucosa withalcohol-inducedinjury (n 8) 6 KGM P450 Table6 Theefectsofkonjacgum powderoncontentsof ADHALDHandP450inmiceliverwithacuteal- coholism (n 8) ADH / ALDH / P450 / ( μ g L -1 Pro) ( μ g L -1 Pro) (pmol L -1 Pro) 7.359±0.452 b 5.750±1.264 b 108.226±11.905 a 8.093±1.608 b 6.121±1.375 ab 73.629±19.814 b 7.794±0.669 b 6.460±1.303 ab 77.763±15.230 b 11.529±2.362 a 7.879±1.094 ab 94.583±13.433 ab 12.119±3.167 a 8.105±2.502 a 108.859±32.403 a KGM 图 5 KGM 对酒精致胃黏膜损伤小鼠血清 PGE2 含量的影响 Figure5 Efectofkonjacgum powderonpge2contentin miceserum withalcohol-inducedinjury(n 8) 160 [31] : ph ( [32] )
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