( )2016 56 4 JournalofNanchangUniversity(MedicalSciences)2016,Vol.56No.4 19,,, (, 510180) : (colageninducedarthritis,cia) 50 (8 ) (42 ) Ⅱ (bovine typeⅡ colagen,cⅡ)+ (completefreund sadjuvant,cfa),1 CⅡ+ (incompletefreund sadjuvant,ifa), CIA 32 CIA 4 :CIA (1 ml 100g -1,2-1 ) 2 ( 1mL 100g -1,1 d -1 ), 8 CIA 0.5% 1mL 100g -1,1 d -1 14 21 28 35 36 -α(tumornecrosisfactor-α,tnf-α) -1β ( interleukine-1β, IL-1β ),CIA, 2 14 21 28 35 36 TNF-α IL-1β ( P<0.05 ); CIA,, 2 21 28 35 36 TNF-α IL-1β ( P<0.05);, 28 35, 36 IL-1β ( P<0.05),,, : ; ; -α; -1β, ; : R285.5 ;R593 : A : 2095-4727 (2016)04-0019-04 DOI:10.13764/j.cnki.ncdm.2016.04.005 EffectsofEmbelinonArthritisScoreandInflammatory CytokinesinRatswithColagen-InducedArthritis YU Na,CAIXiao-yan,LINXiao-jun,TANGChun,YEJing-hua (Departmentof Rheumatology andimmunology,the FirstPeople s Hospitalof Guangzhou,Guangzhou510180,China) ABSTRACT:Objective Toinvestigatetheefectsembelinonarthiritisscoreandinflammatory cytokinesinratswithcolagen-inducedarthritis(cia).methods Fiftyratswererandomlydivid- edintonormalcontrolgroup(n=8)andmodelgroup(n=42).thecia wasinducedbythefirst immunizationwithtype Ⅱ colagen(cⅡ)incompletefreund sadjuvant(cfa)andthesecond immunizationwithcⅡ inincompletefreund sadjuvant(ifa)after1 week.then,32ciarats wererandomlydividedintofourgroups:cia modelgroup,methotrexategroup(1ml 100g -1 in- tragastricadministration,twiceperweek),lowdoseofembelingroup(1ml 100g -1 intragastric administration,onceperday),andhighdoseofembelingroup(1ml 100g -1 intragastricadmin- istration,onceperday),with8ratsineachgroup.ratsinnormalcontrolgroupandcia modelgroup :2015-05-09 : (20151015) : (1976 ),,,,
20 ment.serumconcentrationsoftumornecrosisfactor-α(tnf-α)andinterleukine-1β ( IL-1β ) were detectedonday36oftheexperiment.results Comparedwithnormalcontrolgroup,bodyweight decreasedandarthiritisscoreincreasedondays14,21,28and35,andserum concentrationsof TNF-αandIL-1βincreasedonday36inCIA modelgroup,methotrexategroup,embelingroup, weregivenintragastricadministrationof0.5% sodiumcarboxymethylcelulose1ml 100g -1 once perday.bodyweightandarthiritisscoreweremeasuredondays14,21,28and35oftheexperi- lowdoseofembelingroupandhighdoseofembelingroup(p<0.05).comparedwithcia model group,bodyweightincreasedandarthiritisscoredecreasedondays21,28and35,andserumcon- centrationsoftnf-αandil-1βdecreasedonday36in methotrexategroup,embelingroup,low doseofembelingroupandhighdoseofembelingroup(p<0.05).comparedwith methotrexate group,bodyweightincreasedondays28and35andserumconcentrationsofil-1βdecreasedon day36inhighdoseofembelingroup(p<0.05).conclusion Embelincandecreasethelevelsof inflammatorycytokinesandrelievethesymptomsofarthritisinciarats,suggestingthatembelin maybeefectiveforrheumatoidarthritis. KEY WORDS:embelin;colagen-inducedarthritis;methotrexate;tumornecrosisfactor-α; interleukin-1β ; animals,laboratory;rats, ; (2.5 mg -1 ), [1],,, :036150603;,, :20151019; Ⅱ [2-4] ; (bovinetypeⅡ colagen,cⅡ), (completefreund sadjuvant,cfa), -α(tumornecrosisfactor-α, TNF-α) -1β ( interleukine-1β, IL-1β ) Chondrex, :150394 150355-6(interleukine-6,IL-6), 150366;TNF-α IL-1βELISA Kits [5-6] TNF-α IL-1β RD, FLUKO,, :F6/10;, BIO-TEK, T : μ QuantMQX200,1.3 [7], 1.3.1 CIA, 50 (8 ) [8] (colageninducedarthritis, (42 ), CIA), CIA 2g L -1 CⅡ 0.05mmol CFA 1 1.1 SPF Lewis 50,0.2mL, 0.1mL; 7 CⅡ+ 170~190g, IFA 1, 3cm :SCXK( )2012-0001 0.1 ml Boechat [9], (23±2), (50±10)%, 12h, 0 : ;1 : ( );2 : ( 1 1 );3 : ;4 : 1.2 ( )2016 8, 56 4 (incompletefreund sadjuvant,ifa), CⅡ +CFA, CⅡ +IFA 1 CⅡ+CFA,, CIA 4, ( >98%), 16 2,
: 32, : ELISA TNF-α 76.2%; IL-1β, ELISA Kits 1.3.2 450nm 14, 32 (OD ), TNF-α IL-1β 4 :CIA (0.5% 1mL 100g -1,1 d -1, 21d) 1.4 ( 2.5mg 0.5% SPSS19.0,50 ml,0.05g L -1,1mL 100g -1,2-1, 21d) 2 ( 20 40 mg 0.5%, 2 10mL, 2 4g L -1,1 ml 100g -1,1 d -1, 21d), 8 [10] RA, ( 1A);CIA : (20 mg,, kg -1 ) (40 mg kg -1 ) ( 1B);, 0.5% 1mL 100 g -1, 1 d -1, 21d,, ( 1D); 6 2 1,, CIA, 2 ( 1E) 38 36 1.3.3 36 14, Boe- TNF-α IL-1β ( P<0.05 ); chat [9] CIA,,,, 2 21 28 35 1.3.4 ELISA TNF-α IL-1β : 36 10% ( P<0.05);,, 5mL,3000r 28 35, 36 min -1 15min,, -20, IL-1β ( P<0.05 ) 1 x±s,,2 t, P<0.05 : ( 1C);,CIA, 2 14 21 28 35 36 TNF-α IL-1β 2 21 1 x±s n 14 21 m/g 28 35 8 223.75±7.25 234.00±6.88 245.56±7.89 252.19±6.31 CIA 8 195.93±6.94 * 192.36±10.16 * 195.36±9.31 * 202.36±11.23 * 7 196.00±7.28 * 203.00±9.57 * # 210.13±9.49 * # 216.44±8.13 * # 8 197.06±8.22 * 208.69±7.80 * # 219.56±9.69 * # 227.00±9.71 * # 7 198.43±7.23 * 204.21±10.43 * # 212.86±6.57 * # 220.36±9.73 * # n 14 / 21 28 35 8 0.00±0.00 0.00±0.00 0.00±0.00 0.00±0.00 CIA 8 8.00±1.73 * 10.43±2.07 * 8.71±2.14 * 7.86±1.68 * 7 8.25±1.98 * 7.75±1.49 * # 6.13±1.36 * # 4.63±1.30 * # 8 8.75±1.83 * 7.75±1.58 * # 6.25±1.16 * # 4.13±1.13 * # 7 8.43±1.99 * 7.43±1.40 * # 6.14±1.46 * # 4.71±1.25 * # *P<0.05,#P<0.05 CIA, P<0.05
2 ( )2016 8, 56 4 TNF-α ρ/(ng L -1 ) 80 70 60 50 40 30 20 10 * CIA IL-1β ρ/(ng L -1 ) 120 100 80 60 40 20 * CIA 0 0 3 *P<0.05,#P<0.05 CIA, P<0.05 2 36 TNF-α IL-1β CIA,, RA Sun [8], CIA CⅡ+CFA 1,1 CⅡ + : IFA, 76.2% CIA, [1],,[J].,,,, CIA CIA [11] [6], -κb(nuclearfactorkappab,nf-κb) p65 NF-κB RA [12],RA NF-κBp65, RA NF-κBp65,IL-1β IL-8 TNF-α, NF-κB, CIA TNF-α IL-1β,.,2015,26(12):3011-3013. [2] SahuBD,Anubolu H,Koneru M,etal.Cardioprotectiveefect ofembelinonisoproterenol-inducedmyocardialinjuryinrats: sis[j].lifesci,2014,107(1/2):59-67. (Embelin,2,5- -3- -1,4- ), [2,5-6] 20(10):797-804. CIA,CIA,,, TNF-α IL-1β CIA, IL- 1β, TNF-α IL-1β CIA, Zhao [5] possibleinvolvementofmitochondrialdysfunctionandapopto- [3] NaikSR,NitureN T,AnsariA A,etal.Anti-diabeticactivity ofembelin:involvementofcelularinflammatorymediators,ox- idativestressandotherbiomarkers[j].phytomedicine,2013, [4] KocakC,KocakFE,AkcilarR,etal.Molecularandbiochemi- calevidenceontheprotectiveefectsofembelinandcarnosic acidinisoproterenol-induced acute myocardialinjuryin ra- ts[j].lifesci,2016,147:15-23. [5] ZhaoZ G,TangZZ,Zhang W K,etal.Protectiveefectsof embelinon myocardialischemia-reperfusioninjuryfolowing cardiacarrestinarabbitmodel[j].inflammation,2015,38(2): 527-533. [6] ZhouX L,HuangL,CaoJ.Embelinreducessystemicinflam- mationandamelioratesorganinjuriesinsepticratsthrough downregulatingstat3and NF-κBpathways[J].Inflamma- tion,2015,38(4):1556-1562. [7] SharmaA R,SharmaG,LeeSS,etal.miRNA-regulatedkey componentsofcytokinesignalingpathwaysandinflammation inrheumatoidarthritis [J].MedResRev,2016,36(3):425-439. ( 64 )
64 ( )2016 8, 56 4 1 205 n 205 172 13 11 4 2 2 1 205 175 13 12 3 1 0 1 /% 98.3 100.0 91.7 75.0 50.0 0.0 100.0 3,,,,, [3],,, X,,, :, [4] [1],,,.,X MRI, 3~5, [5],,, 2015,28(4):31. [4],,,, 63. [5], [6] 98.3%, [J].,2014,11(16):2279-2280. 97.2% [7] [6], 99.45% [8] 100.0% 72., [7],,,, [8],,, [7],,, : 32 2003 2007 [J].,2012,32(6):435-439. [2],,,. [J].,2015,24(1):73-76. [3].5806 [J].,..3 [J].,2015,53(7):60-. : 1394 [J].,2015,16(12):71-. 108 [J].,2014,21(32):182-184.,,. : 1394 [J]., 2014,34(7):660-662. ( : ) 櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐櫐 ( 22 ) [8] SunY,YaoY,DingCZ.Acombinationofsinomenineandmetho- trexatereducesjointdamageofcolageninducedarthritisinratsby modulatingosteoclast-relatedcytokines[j].intimmunopharma- col,2014,18(1):135-141. [9] BoechatA L,deOliveiraCP,TarragA M,etal.Methotrexate- loadedlipid-corenanocapsulesarehighlyefectiveinthecontrol ofinflammationinsynovialcelsandachronicarthritismodel [J].IntJNanomedicine,2015,10:6603-6614. 7,10. [10],,,. [J].,2015,12(1):5- [11],,. Ⅱ [J].,2015,25(4): 48-57. [12],,. -κbp65 [J].:,2014,54(1):10-14. ( : )
蒽贝素对大鼠胶原诱导性关节炎炎症评分及炎症细胞因子的影响 正文见第 １９ 页 B A D C E Ａ 正常对照组 Ｂ ＣＩＡ 模型组 Ｃ 甲氨蝶呤组 Ｄ 蒽贝素高剂量组 Ｅ 蒽贝素低剂量组 图 １ 各组大鼠后足形态学的变化 Ｐ ｉ ｌ ｉ ｎｅ血流导向装置治疗颅内大型动脉瘤 １ 例 正文见第 １０３ 页 ｐｅ A B C D E F G H I Ａ 颅脑磁共振血管造影显示颈内动脉海绵窦段动脉瘤 Ｂ 颅脑颅脑磁共振血 管 造 影 示 颈 内 动 脉 海 绵 窦 段 动 脉 瘤 Ｃ 数 字 减 影 血 管造影显示 颈 内 动 脉 海 绵 窦 段 动 脉 瘤 Ｄ 数 字 减 影 血 管 造 影 显 示 颈 内 动 脉 海 绵 窦 段 动 脉 瘤 Ｅ 血 流 导 向 装 置 支 架 释 放 后 即 刻 动 脉 瘤 显影变淡 Ｆ 血流导向装置支架释放后即刻动脉瘤显影变淡 Ｇ 透 视 下 血 流 导 向 装 置 清 晰 显 影 红 色 箭 头 所 指 Ｈ 数 字 减 影 血 管 造 影显示动脉瘤完全不显影且载瘤动脉光滑通畅 早期 Ｉ 数字减影血管造影显示动脉瘤完全不显影且载瘤动脉光滑通畅 晚期 图 １ 颅内大型动脉瘤的血管造影表现