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1 South China Fisheries Science Vol. 11, No. 6 Dec., 2015 doi: / j. issn ,,,, (,, ) :,,,,, : ; ; : S : A : ( 2015) Research progress of intestinal microbial flora in shrimp ZHANG Jiasong, DUAN Yafei, ZHANG Zhenzhen, DONG Hongbiao, LI Zhuojia ( Key Lab. of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou , China) Abstract: The intestinal structure and function of microbial flora, which play an important role in shrimp growth, are closely related to the physiological process of nutrition metabolism, energy balance, immune defense and gastrointestinal development, and are essential to maintain the stability of internal environment. The paper reviews the nutrition, digestion, immune and physiological function of shrimp intestinal microbial flora, analyzes the influence factors of the shrimp intestinal microbial flora structure and composition, and introduces the main research method of shrimp structure of shrimp intestinal microbial flora, so as to put forward the prospects of its application in shrimp aquaculture. Key words: shrimp; intestinal microbial; flora,,,,,,, 1 1-2],,,, 3] 4-6 ],,, : ; : : ( 2011BAD13B10) ; ( ) ( 2014TS15, 2012 YD02) ; ( A201101E02, A201208H01, A201201B04) : ( ),,,, jiasongzhang@ hotmail. com

2 6 : ,,,,, 1. 1,,,, 22] 2) 7-8],,, ;,, 23] 3), ph,,, 9-10] KIMIAKI 11] 24],,,,,, 1. 2,,, 25] ( WSSV),,, ( Lactobacillus) ( Marinococcus) ( Vibrio) ( Aeromonas),, ( Salinicoccus),,, 16], 13-14],, ( Bacillus subtilis), ( Litopenaeus vannamei) 15] 16] SE TIE,,,, 17-18], ; 1. 3 ( ),, 2. 1, : 1), ; 2),, 19],,,, 13],,, 9], 26-28],, 20-21],, 2. 2 : 1),

3 116 11, ,,, 29-30], ph, 36-37],, ph ( ),, 38],,,,,,, 15, 39],,,,, 31], ,,,, 32] 27 ],,,, 3. 1,, ( Bifidobacterium) ; 33] ,,, 90%, ( ),,, 9],,, 40], ;,,, ] BE ER 34] 2, 16S rrna,, 16S rdna 11 10,,, 16S rdna,, ZHANG 35],, 41] , 16S rdna, ( denaturing gradient gel electrophoresis,,, DGGE) ( temperature gradient gel electro-,, phoresis, 3, TEEG) ( termi- (Marsupenaeus japonicus), nal restriction fragment length polymorphism, ( Arthrobacter), ( single strand conformation polymorphism, 29],, SSCP) PCR ( length hetetogeneity PCR, T-RFLP),, PCR) LH-

4 6 : ], JO HANDELSMAN1998,,,, DNA : DNA PCR,, 41] ( 1),,,,,,, ;, 1,,, 1 Fig. 1 ( 40] ) Flowchart of metagenomics research Tab. 1 1 Intestinal bacterium group in L. vannamei by different research methods method Solexa Solexa sequencing intestinal microbial flora composition Reference 64. 1%, 35. 5%, 0. 4% 43] Biolog Biolog and ( Photobacterium) ( Pseudomonas) 44] medium cultivation medium cultivation ( Enterobacteriaceae) 45] ( Xanthomonas) ( Agrobacterium) 16S rdna ( Shewanella) ( Pantoea) Aranicola 16S rdna-rflp 46] DGGE DGGE fingerprint 12, 2, 3 28]

5 ] HOSEINIFAR S H, SHARIFIAN M, VESAGHI M J, et al. The, effects of dietary xylooligosaccharide on mucosal parameters, intestinal microbiota and morphology and growth performance of Caspian, white fish ( Rutilus frisii kutum) fry J]. Fish Shellfish Immunol, 2014, 39( 2 ) : , 13] ALMANSA C, AGRAWAL A, HOUGHTON L A. Intestinal mi-, crobiota, pathophysiology and translation to probiotic use in pa- tients with irritable bowel syndrome J]. Expert Rev Gastroenterol, Hepatol, 2012, 6 ( 3) : ],,,., J]., 2013( 12) : , 15] ZOKAEIFAR H, BALC ZAR J L, SAAD C R, et al. Effects of, Bacillus subtilis on the growth performance, digestive enzymes, immune gene expression and disease resistance of white shrimp, Litopenaeus vannamei J]. Fish Shellfish Immunol, 2012, 33 : 1] BID A, DAVIES S J, WAINES P, et al. Dietary symbiotic application modulates Atlantic salmon ( Salmo salar) intestinal microbial communities and intestinal immunity J]. Fish Shellfish Immunol, 2013, 35( 6 ) : ] CAHENZLI J, YASMIN K Y, WYSS M, et al. Intestinal microbial diversity during early-life colonization shapes long-term Ig E levels J]. Cell Host Microbe, 2013, 14 ( 5) : ],,,. ( 3) : J]., 2014, 38 ( 9 ) : ] RAMOS M A, WEBER B, GONCALVES J F, et al. Dietary probiotic supplementation modulated gut microbiota and improved growth of juvenile rainbow trout ( Oncorhynchus mykiss) J]. Biochem Physiol A, 2013, 166( 2) : Comp 5],,. 21] AGUILERA-RIVERA D, PRIETO-DAVA, ESCALANTE K, J]., 2013, 19( 4 ) : J]., 2010, 50( 7) : ] SEMOVA I, CARTEN J D, STOMBAUGH J, et al. Microbiota regulate intestinal absorption and metabolism of fatty acids in the zebrafish J]. Cel Bl Host Microbe, 2012, 12( 3 ) : ] ZHANG M L, SUN Y H, CHEN K, et al. Characterization of the intestinal microbiota in Pacific white shrimp, Litopenaeus vannamei, fed diets with different lipid sources J]. Aquaculture, 2014, 434: , 2013, 25 ( 3) : ] HAIYAPECHARA S, RUNGRASSAMEE W, SURIYACHAY I, et al. Bacterial community associated with the intestinal tract of P. monodon in commercial farms J]. Microb Ecol, 2012, 63 ( 4 ) : ] KIMIAKI Y, TADATOSHI K. Bacterial flora in the digestive tract of prawn, Penaeus japonicus Bate J]. Aquaculture, 1980, 19 : ( 4) : ]. D]. :, 2004: ]. D]. :, 2011 : ],. J]., 2010, 31 ( 20 ) : ],,,. J]., 2013, 19 20] ZOKAEIFAR H, BABAEI N, SAAD C R, et al. Administration of Bacillus subtilis strains in the rearing water enhances the water quality, growth performance, immune response, and resistance a- gainst Vibrio harveyi infection in juvenile white shrimp, Litopenaeus vannamei J]. Fish Shellfish Immunol, 2014, 36 ( 1 ) : et al. Probiotic effect of FLOC on Vibrios in the pacific white 6],,,. PCR-DGGE shrimp Litopenaeus vannamei J]. Aquaculture, 2014, 424 /425 : ],,,. J] ] HEMPEL S, NEWBERRY S J, MAHER A R, et al. Probiotics for the prevention and treatment of antibiotic-associated diarrhea: systematic review and meta-analysis J ]. JAMA, 2012, 307 ( 18 ) : ] SHA S, XU B, WANG X, et al. The biodiversity and composition of the dominant fecal microbiota in patients with inflammatory bowel disease J]. Diagn Microbiol Infect Dis, 2013, 75 ( 3 ) : 24] DESRIAC F, DEFER D, BOURGOUGNON N, et al. Bacteriocin as weapons in the marine animal-associated bacteria warfare: inventory and potential applications as an aquaculture probiotic J]. Mar Drugs, 2010, 8 ( 4) : ] PELUCCHI C, CHATENOUD L, TURATI F, et al. Probiotics a

6 6 : 119 supplementation during pregnancy or infancy for the prevention of atopic dermatitis: a meta-analysis J]. Epidemiology, 2012, 23 ( 3) : ],,,. RFLP DGGE J]. 2014, 434: , 2014, 35( 2) : ],,,. J]., 2008, 4 ( 6) : J]., 2014, 38 ( 5 ) : ]. 38],,. D]. :, J]., 2010: ] RUNGRASSAMEE W, KLANCHUI A, CHAIYAPECHARA S, et al. Bacterial population in intestines of the black tiger shrimp ( Penaeus monodon) under different growth stages J]. PLoS ONE, 2013, 8( 4) : ] RUNGRASSAMEE W, KLANCHUI A, MAIBUNKAEW S, et al. Characterization of intestinal bacteria in wild and domesticated adult black tiger shrimp ( Penaeus monodon) J]. 2014, 9( 3) : PLoS ONE, 31],,,. J]., 2014, 41( 1) : PCR-DGGE J]., 2006, 25( 2) : ],,,. 44],,,. J]., 2015, 24 ( 2) : ],,. 45],,,. J]., 2004, 28 ( 5) : ] BE ER A, ZHANG H P, FLORIN E L, et al. Deadly competition between sibling bacterial colonies J]. PNAS, 2009, 106 ( 2) : ] ZHANG M L, SUN Y H, CHEN K, et al. Characterization of the intestinal microbiota in Pacific white shrimp, Litopenaeus vannamei, fed diets with different lipid sources J]. Aquaculture, 36],,,. 37],,,. J]., 2006, 2( 4) : , 30( 7 ) : ],,,. J]., 2010, 29( 4) : ],,. J]., 2014, 35( 4) : ],,,. J]., 2009, 29 ( 5) : ],,,. 43]. D]. :, 2011: Litopenaeus vannamei J]., 2005, 1( 3) : J]., 2006, 25 ( 1) : ],,,. J]., 2007, 47( 4 ) :

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<4D6963726F736F667420576F7264202D20313031A67EABD7AEFCAC76BAF1AAF7AF53A6E2A8E5BD64ADD7A5BFABE1AD70B565AED15FBCEAACECA46A313031303431305F2E646F63> 教育部補助技專校院建立特色典範計畫一一年度具體計畫書海洋綠金 - 澎湖海洋經濟性藻類及週邊產業特色典範計畫 Education and Technique Developments for Taiwan Green Gold-Marine Economic Seaweeds and Associated