Microsoft Word _编辑二校出3133

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1 7 8 Vol. 7 No Journal of Food Safety and Quality Aug., 2016 徐玲玲, 李卫群, 朱慧 *, 汪涓涓 (, ) 摘要 : 目的 Adulterant Screen 方法, Adulterant Screen 结果, 0.3 g/100g 0.2 g/100g, 0.5 g/100g 0.8 g/100g 结论 Adulterant Screen 关键词 : ; ; ; ; Detection of adulteration of milk powder by near infrared spectroscopy XU Ling-Ling, LI Wei-Qun, ZHU Hui *, WANG Juan-Juan (Hangzhou Wahaha Group Co., Ltd., Hangzhou , China) ABSTRACT: Objective To establish a method for detection of the soybean protein and urea adulterated in milk powder rapidly by near infrared spectroscopy (NIRS) combined with Adulterant Screen algorithm. Methods The spectroscopic curves of unknown milk powder samples were obtained by means of NIRS, and then the main ingredients and the adulterated ingredients from the acquired spectroscopic curves were analyzed based on the Adulterant Screen algorithm, the full database milk powder model and the established adulterated ingredients model. Results The method was able to identify the soybean protein and urea adulterated milk powder with a certain concentration. The adulteration detection limits of this method were 0.3 g/100 g and 0.2 g/100 g, and the adulterated ingredient correct recognization limits were 0.5 g/100g and 0.8 g/100 g for soybean protein and urea, respectively. Conclusion The method can rapidly recognize the adulteration of the soybean protein and urea in milk powder by NIRS combined with Adulterant Screen algorithm. KEY WORDS: near infrared spectroscopy; milk powder; adulteration; soybean protein; urea 1 引言,,,,, 基金项目 : ( ) (2015C37074) Fund: Supported by Public Welfare Technology Applied Research Projects in Zhejiang Province Project (2015C37074) * 通讯作者 :,,, zhuh@wahaha.com.cn *Corresponding author: ZHU Hui, Professor Senior Engineer, Hangzhou Wahaha Group Co., Ltd., Hangzhou , China. zhuh@wahaha.com.cn

2 [1,2] [3-5],,,,,,, (near infrared spectroscopy, NIR) [6-13], NIR [14-17] / 50 ( ), FTIR, Adulterant Screen 1 g/100 g DairyGuard [18,19] :, ;, 2 材料与方法 2.1 仪器设备 DairyGuard ( FrontierNIR, NIRAⅡ, Perkin Elmer ) 20 s, 16 cm min, 2.2 样品来源光谱的获取及模型库的建立全数据库奶粉分类模型的建立 50, DairyGuard, 25, 25 ( ) 既定类型的掺假物模型的建立, 模型库的建立, 2.3 光谱数据预处理,,, 9, 2.4 Adulterant Screen Adulterant Screen SIMCA,, [18] :, (principal component analysis, PCA) ;, PCA ;,,,,,, Adulterant Screen,,,,, 样品制备及检测 g/100 g,, 3,,, g/100 g, 3 3 结果与分析 3.1 不同大豆蛋白含量掺杂奶粉样检测结果 DairyGuard,,, PCA,,,,,,,,, 1 1,, 0.5 g/100 g

3 8, : 3135, 0.5 g/100g,, 3, 0.5 g/100 g,,, 1/3 0.2 g/100g 3.2 不同尿素含量掺杂奶粉样检测结果 2 2,, 0.1 g/100g,, 0.2 g/100g,,, - 1/3 0.5 g/100 g, 2/3, 0.8 g/100 g, 3.3 不同物质的近红外光谱 1 1,, ( B) 0.8 g/100 g ( A),, Adulterant Screen, :, ;, ( C), 2 2, 0.8 g/100 g ( E) ( F), E F,, Adulterant Screen, :, ;, ( D),, 2, (0.2 g/100 g) (0.3 g/100 g) Table 1 表 1 掺有不同含量大豆蛋白的奶粉样品的检测结果 Detection results of milk powder adulterated different concentrations of soybean protein (g/100g) / / / / /3 Table 2 表 2 掺杂有不同尿素含量的奶粉样品的检测结果 Detection results of milk powder adulterated different concentrations of urea (g/100g) / / / /3

4 ( ) Fig.1 The near infrared spectrogram of different substances (soybean protein as adulteration) 2 ( ) Fig.2 The near infrared spectrum of different substances (urea as adulteration) 4 结论 DairyGuard Adulterant Screen, 6 5, 0.3 g/100g, 0.8 g/100g, 0.2 g/100 g, 0.8 g/100g DairyGuard 3, DairyGuard 参考文献 [1]. [J]., 2000, (3): 53. Zhang YY. Sensory identification of milk and milk quality [J]. Food Sci, 2000, (3): 53. [2]. [J]., 2005, (4): 36. Zhong Z. Quality identification of milk powder [J]. Guangxi Qual Superv Guide Period, 2005, (4): 36. [3],,,. LS-SVM [J]., 2008, 27(3): Wu D, He Y, Feng SJ, et al. Application of infrared spectra technique based on LS-support vector machines to the non-destructive measurement of fat content in milk powder [J]. J Infrared Millimeter Waves, 2008, 279(3): [4] Wu Di, He Yong, Feng SY. Short-wave near-infrared spectroscopy analysis of major compounds in milk powder and wavelength assignment [J]. Anal Chim Acta, 2008, 610: [5] Borin A, FerrgLo MF, Mello C, et a1. Least-squares support vector machines and near infrared spectroscopy for quantification of common adulterants in powdered milk [J]. Anal Chim Acta, 2006, 579: [6] He Y, Wu D, Feng S, et a1. Fast measurement of sugar content of yogurt

5 8, : 3137 using Vis/NIR spectroscopy [J]. Int J Food Prop, 2007, 10(1): 1 7. [7] Kasemsumran S, Thanapase W, Kiatsoonthon A. Feasibility of near-infrared spectroscopy to detect and to quantify adulterants in cow milk [J]. Anal Sci, 2007, 23(7): 907. [8] Balabin RM, Smirnov SV. Melamine detection by mid-and near-infrared(mir/nir)spectroscopy: a quick and sensitive method for dairy products analysis including liquid milk, in rant formula, and milk powder [J]. Talanta, 2011, 85(1): [9],,,. CARS [J]., 2010, 29(5): Zhang HX, Li XN, Fan W, et al. Determination of protein and fat in liquid milk by NIR combined with CARS variables screening method [J]. J Instrum Anal, 2010, 29(5): [10],,,. [J]., 2010, 31(1): Tang YL, Liang YZ, Fan W, et al. Fast discrimination of varieties of different age rank milk powder using near infrared spectroscopy [J]. Infrared, 2010, 31(1): [11]. [J]., 2010, 33(3): Tang YL. Fast Discrimination of infant and middle-aged milk powder using near infrared spectra infrared spectra with linear discriminant analysis [J]. J Dairy Sci Technol, 2010, 3(3): Jin H, Li D, Zhi XJ. Detection of melamine concentration in milk powder based on NIR technology [J]. J Beijing Univ Agric, 2015, 30(1): [15],,,. [J]., 2015, 35(8): Wang NN, Shen BH, Guan JJ, et al. Detection of adulteration in milk powder with starch near infrared [J]. Spectrosc Spect Anal, 2015, 35(8): [16] Borin A, Ferro M F, Mello C, et al. Least-squares support vector machines and near infrared spectroscopy for quantification of common adulterants in powdered milk [J]. Anal Chim Acta, 2006, 579(1): [17] Balabin RM, Smirnov SV. melamine detection by mid-and near-infrared (MIR/NIR) spectroscopy: A quick and sensitive method for dairy products analysis including liquid milk, infant formula, and milk powder [J]. Talanta, 2011, 85: [18] Ben Perston, Rob Packer. DairyGuard: Augmenting nutritional testing of milk powder with Adulterant Screening [R]. Shelton, CT: PerkinElmer Inc., 2015: 1 6. [19] Ian Robertson. Use of NIR spectroscopy and adulterant screen for the detection of common adulterants in milk [R]. Shelton, CT: PerkinElmer Inc., 2015: 1 3. ( 责任编辑 : 姚菲 ) 作者简介 [12], 玥, [J], 2004, 37(12): Lv LN, Zhang Y, Zhou DW. Composition analysis of milk by using near infrared diffuse reflection spectroscopy [J]. J Tianjin Univ, 2004, 37(12) : 徐玲玲, 硕士, 主要研究方向为光谱分析 zjlgxll@163.com [13],,,. [J]., 2015, (1): Wang M, Yu F, Liu X, et al. Detection of protein,fat in milk by using near infrared diffuse reflectance technique [J]. Laser J, 2015, (1): [14],,. NIR [J]. 朱慧, 教授级高工, 主要研究方向为光谱分析 @qq.com, 2015, 30(1):

30期

30期中国农学通报 2014,30(30):285-289 Chinese Agricultural Science Bulletin 傅里叶变换红外光谱法在橄榄油掺假鉴别中的应用黄秀丽, 黄飞, 曾宪远, 宁焕焱, 唐丽娜, 奉夏平 ( 广东省惠州市质量计量监督检测所, 广东惠州 516003)

Microsoft Word _编辑 二校出3133

Microsoft Word _编辑二校出3133