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1 72 Chinese Toco Science * LC1 POD 3 12 h CAT 3 S DOI /j.issn Eects o Conventionl Insecticides on Aphidius Giuensis Ashmed nd Its Protective Enzymes in Toco Fields ZHU Xinzhi 1, LIU Yong 1, TIAN Lei 2, LIU Li 1, ZONG Ho 2, GAO Qing 1*, XU Rui 1, LI Lnen 1, ZHANG Wein 1 (1. Linyi Toco Compny o Shndong Province, Yishui, Shndong 2764, Chin; 2. Linyi Toco Compny o Shndong Province, Yishui Brnch Linyi, Shndong 276, Chin ) Astrct: In order to screen or ser insecticides to Aphidius giuensis Ashmed nd know out the eects o conventionl insecticides on protective enzymes o Aphidius giuensis Ashmed in toco ields, toxicity o three conventionl insecticides to Aphidius giuensis Ashmed ws determined vi the residul ilm method. Eects o dierent concentrtions o imidcloprid on SOD, POD nd CAT ctivities o Aphidius giuensis Ashmed ws lso determined. The results showed tht the order rom high toxicity to low toxicity ws: cetmiprid >lmd-cyhlothrin >imidcloprid. In terms o the eects o dierent concentrtions o imidcloprid on the lie ctivities o Aphidius giuensis Ashmed nd their deense mechnisms, ll the tretments showed inhiition on enzyme ctivities except tht the LC1 concentrtion o imidcloprid improved POD ctivities in dults nd CAT ctivities incresed signiicntly in dults ter 12h tretment o three concentrtions. This result provides theory sis or understnding the deense mechnisms o Aphidius giuensis Ashmed ginst insecticides. Keywords: insecticides; Aphidius giuensis Ashmed; toxicity; enzyme ctivity Myzus persice Sulzer Aphidius giuensis Ashmed KN227 E-mil @163.com * E-mil lyysgq@139.com

2 3 73 [1-2] Superoxide dismustse SOD Peroxidse PODCtlse CAT [3-5] 3 [6-9] 3 [1-11] ~27 5%~8% 24 h g/l 2 g/l 5% ~6 3 [12].2 ml 4 cm 15 cm 1% 2 2 h 25 8% L//D=12 h//12 h 24 h Aott 2% Aott 处理组死亡率 % - 对照组死亡率 % 校正死亡率 1% 1- 对照组死亡率 % LC mg/l LC mg/l LC mg/l LC mg/l h SOD [13] POD [14-15] CAT [14] 1.3 DPS LC 5 95%

3 Duncn g/l LC mg/l 25 g/l mg/l 5% mg/l h T1e 1 Toxicity o 3 insecticides to dults o Aphidius giuensis Ashmed y sprying method (24 h ter drug) /n ± LC5/ mg L -1 95% ± ~ ± ~ ± ~ SOD h mg/l h SOD mg/l 24 h 3 12 h SOD U/mg 36.57% 41.3% 52.49% SOD 48 h mg/l SOD SOD 5.89% 35.22% 5.23 mg/l 52.49% 12.99% SOD SOD SOD /(U mg -1 min -1 ) g cd c /h 5% 1 SOD Fig. 1 Chnge in SOD ctivities in dults o Aphidius giuensis Ashmed ter exposure to imidcloprid or dierent time 2.3 POD h POD mg/l h POD 48 h POD 1.69 U/(mg min) 56.48% 5.23 mg/l h h 12 h 6.19% mg/l POD 2.4 CAT h CAT mg/l 12 h CAT U/(mg min) 66.99% 58.1% 5.23 mg/l 12 h CAT 48 h e c de c

4 3 75 POD 活性 /(U mg- 1 min -1 ) cde 处理时间 /h de e e 2 POD Fig. 2 Chnge in POD ctivities in dults o Aphidius giuensis Ashmed ter exposure to imidcloprid or dierent time CAT /(U mg -1 min -1 ) cd d /h d d e 3 CAT Fig. 3 Chnge in CAT ctivities in dults o Aphidius giuensis Ashmed ter exposure to imidcloprid or dierent time U/(mg min) 3.12% 38.96% 53.15% CAT g/l 5% 25 g/l [16] [17] 3 d 7 d Suh [18] 2 g/l POD CAT [22] SOD [16] SOD POD CAT [7,19] [2] 3 SOD CAT SOD CAT [21] SO 2 SO 2

5 g/l 5% 25 g/l [1] 曌. [J] [2]. [J] [3] Allen R G, Frmer K J, Sohl R S. Eect o dimide dministrtion on longevity, oxygen consumption, superoxide dismutse, ctlse, inorgnic peroxides ndglutthione in dult house ly[m]. Musc domestic. Comp. Biochem. Physiol. C, 1984, 78: [4] Allen R G, Blin A K. Oxidtive inluence ondevelopment nd dierentition: n overview o reerdicl theory o development[m]. Free Rdic. Biol. Med., 1989, 6(6): [5] Bolter C J, Cheurk W. Extrmitochondril relese o hydrogen peroxide rom insect nd mouse liver mitochondriusing the respirtory inhiitors phosphine myxothizol ndntimycin nd spectrl nlysis o inhiited cytochromes[m]. Arch. Biochem. Biophys, 199, 278 (1): [6]. B [J] [7]. [J] [8] 囷. [J] [9]. Dh [J] [1]. [J] [11]. [J] [12]. NY/T [S]. 27. [13]. [J] [14]. [M]. 2. [15] Ginnopolitis C N, Ries S K. Superoxide dismutses. I. Occurrence in higher plnts. Plnt[J]. Physiology, 1977(59): [16]. [J] [17]. [J] [18] Suh C P, Qrr D B, Duyn J W, et l. Eect o insecticides on Trichogrmm exiguum (Trichogrmmtide: Hymenopter) preimginl development nd dult survivl[j]. Journl o Economic Entomology, 2, 93(3): [19]. - [J] [2]. SODCAT [D] [21]. SO2 [J] [22]. [J]

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