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1 7 9 Vol. 7 No Journal of Food Safety and Quality Sep., 2016 C 李启彭 1 1,, 申昆 2, 罗家刚 1, 师睿 1, 沈燕琼 (1., ; 2., ; 3., ) 3* 摘要 : 目的 C, 方法 C, 2,4- 结果 : 37, 3 h 1.0 g 497 nm C mg/100 g 结论 C 关键词 : 2,4-; ; C Extraction technology and determination of vitamin C in characteristic fruits from Zhaotong LI Qi-Peng 1, SHEN Kun 1, 2, LUO Jia-Gang 1, SHI Rui 1, SHEN Yan-Qiong 3* (1. College of Chemistry and Life Science, Zhaotong University, Zhaotong , China; 2. Junior Middle School of Liuquhe Town, Bijie , China; 3. Agricultural Product Quality and Safety Center of Zhaotong, Zhaotong , China) ABSTRACT: Objective To optimize the extraction of vitamin C in characteristic fruits from Zhaotong and determine its content. Methods The single factor experiment was designed to optimize and extract vitamin C from Zhaotong characteristic fruits, and their content were determined by the 2,4-dinitrobenzene hydrazine method and ultraviolet spectroscopy. Results The best extraction conditions were as follows: the extraction temperature was 37, the time of water bath was 3 h and the dosage of the activated carbon was 1.0 g. Using the ascorbic acid as the standard solution, the vitamin C content of hawthorn, pear, apple and grape from Zhaotong were 30.3, 6.3, 7.1 and 25.8 mg/100 g, respectively. Conclusion The method can provide a reference for extraction and determination of vitamin C content from Zhaotong characteristic fruits. KEY WORDS: 2,4-dinitrobenzene hydrazine method; Zhaotong characteristic fruits; vitamin C 1 引言 C,, 100 mg C, C [1-4], C, [5] C,, 基金项目 : (2016xj35) Fund: Supported by the School Project of Zhaotong University (2016xj35) * 通讯作者 :,,, @qq.com *Corresponding author: SHEN Yan-Qiong, Assistant Agronomist, Agricultural Product Quality and Safety Center of Zhaotong, No.47, Fengxia Road, Zhaoyang District, Zhaotong , China @qq.com

2 [6,7], C 2,6-2,4-, [8-10],,,,,,, C, C,, 2 材料与方法 2.1 材料 试剂与仪器 ( ) ( ); 2,4- (, ), () Unico UV/VIS 2802PCS ( ); DZKW-S-4 ( ); Ohaus CP214 ( ) 2.2 实验方法 标准溶液的配制 g, 1%, 50 ml 1.0 g 25 ml, 2 min, 5 ml 1% 250 ml, 20 ug/ml 20 ug/ml ml 25 ml, 1% 水果鲜样处理,,, g, 1% 10 ml, 10 ml 100 ml, 1%, 10 min 4 20 ml, 1.0 g,, 1 min, 1:1, 2% 8 (, ), 4 ml, 2% 2,4-1 ml,, 3 h,, 20 min,, 5 ml 85%,, 1 ml 2% 2,4-, 5 ml 85%, 20 min,, 497 nm 3, 2.3 选择最大吸收波长及标准溶液测试 4 ml,, 1 ml 2% 2, h, 1 ml 2% 2,4-,, 5 ml 85%,, 20 min, 400~600 nm A, C (ug/ml), 2.4 单因素提取实验 反应温度的影响 10, 1.0 g, 3 h, , 497 nm, 水浴时间的影响 7 37, 1.0 g, h, 497 nm, 活性炭用量影响 7, 37 3 h, g, 497 nm, 3 结果与讨论 3.1 测定波长的选定及标准曲线绘制,, 497 nm ;,, 497 nm ( 1) 497 nm, A,,, : A = C, r , 1~12 μg/ml A ( 2)

3 9, : C 3685 Fig. 1 1 The UV spectrum of vitamin C solutions 3 (n=3) Fig.3 The absorbance under different temperatures (n=3) Fig. 2 2 The standard curve of vitamin C solutions 4 (n=3) Fig.4 The absorbance of different reaction time (n=3) 3.2 单因素实验结果 反应温度, 25~45,,, 37, ; 37,, 37 ( 3) 反应时间,, 1.5~4.0 h,, 3.0 h, 3.0 h,, 3.0 h ( 4) 活性炭加入量 3.0 h, 37,,,, 1.0 g,, 1.0 g ( 5) 3.3 样品的测定及加标回收率,, C, 497 nm,, C 30.3 mg/100g, 25.8 mg/100g, 7.1 mg/100g, 6.3 mg/100g( 1),, 5 mg

4 3686 7, mg/g, 97%, C,,, [11-14],, [15] 4 结论 C,,, C, 5 (n=3) Fig.5 The relationship between the absorbance and the amount of activated carbon (n=3) Table 1 表 1 苹果 山楂 梨和葡萄中维生素 C 含量测定值 (n=3) Determination of vitamin C in apple, hawthorn, pear and grape (n=3) (g) A (mg/g) (mg/g) RSD (%) 参考文献 [1] Edefonti V, Hashibe M, Parpinel M, et al. Natural vitamin C intake and the risk of head and neck cancer: a pooled analysis in the International Head and Neck Cancer Epidemiology consortium [J]. Int J Cancer, 2015, 137(2): [2]. [J]., 2016, 6(43): Ye QL. Determination of vitamin C content in fruits by spectrophotometry [J]. Guangdong Chem, 2016, 6(43): [3] Gao Y, Han Z, Li Q et al. Vitamin C induces a pluripotent state in mouse embryonic stem cells by modulating micro-rna expression [J]. FEBS J, 2015, 282(4): [4]. C [J]., 2008, 37(6): Zhao LJ. Determination of Vc content of fruits by UV colorimetry [J]. Contemp Chem Ind, 2008, 37(6): [5] Popovic LM, Mitic NR, Miric D, et al. Influence of vitamin C supplementation on oxidative stress and neutrophil inflammatory response in acute and regular exercise [J]. Oxidat Med Cell Longevity, 2015:1 7. [6],,,. 7 C [J]., 2010, 39(4): Chen SD, Chen FB, Yang YM. et al. Determination of Vitamin C in seven southern fruit [J]. Technol Dev Chem Ind, 2010, 39(4): [7] Park S, Ham J O, Lee B-K. Effects of total vitamin A, vitamin C, and fruit intake on risk for metabolic syndrome in Korean women and men [J].

5 9, : C 3687 Nutrition, 2015, 31(1): [8] GB/T C (2, 6- ) [S]. GB/T Determination of vitamin C In vegetables and fruits (2. 6-di chloro-indophenolt itration method) [S]. [9],,. C [J]., 2011, 50(11): Sun P, Wang N, Sun XF. Comparison of two methods for detecting vitamin C in apple [J]. Hubei Agric Sci, 2011, 50(11): [10]. 2,4- C [J]., 2015, 37(2): Xu ML. A research on measuring content of vitamin C in three vegetables by 2, 4-Dinitrophenylhydrazine method [J]. J Heze Univ, 2015, 37(2): [11],,,. C [J],, 2010, 1(3): Zhang DM. Wang ZL. Luo LB et, al. Study on the influencing factors of the determination results of vitamin C in fresh fruits and vegetables [J]. Jiangxi Chem Ind, 2010, 1(3): [12],,,. C [J]. -, 2007, 43(4): Huang SY, Fang XY. Nong SY et al. Spectrophotometric determination of vitamin C in hawthorn fruit [J]. Phys Chem Inspect (Part B: Chem Anal), 2007, 43(4): [13],,,. 7 C [J]., 2012, 34(1): Huang LY, Cheng CL, Luo B, et. al. Determination of vitamin C content of 7 common fruits in the west of Guangxi [J]. J Youjiang Med Univ Nation, 2012, 34(1): [14],,,. C [J]., 2015, 9(3): Yang JM, Qing CF, Yu R, et al., The spectropho to determination for vitamin C in pears and apples [J]. Anhui Med Pharm J, 2015, 9(3): [15],. [J]., 2016, 7(2): Shen YQ, Li QP. Optimization of extraction technology and determination of flavonoid in Huize radix puerariae [J]. J Food Saf Qual, 2016, 7(2): 作者简介 ( 责任编辑 : 姚菲 ) 李启彭, 博士, 讲师, 主要从事无机及分析化学研究 @qq.com 沈燕琼, 硕士, 助理农艺师, 主要从事特色农产品营养成分及农残研究 @qq.com