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1 Chinese Journal of Applied Entomology 2017, 54(3): DOI: /j.issn * 芦荟提取物对桔小实蝇产卵驱避活性研究 ** 李智伟 *** 刘家莉熊婷曾鑫年摘要目的 Aloe vera L. Bactrocera dorsalis Hendel 方法 5 5 结果 5 10 mg/ml % % 5 6 结论关键词 Aloe vera L. extracts repel oviposition by the oriental fruit fly (Bactrocera dorsalis) LI Zhi-Wei ** LIU Jia-Li XIONG Ting ZENG Xin-Nian *** (College of Agricultural, South China Agricultural University; Guangdong Engineering Research Center for Insect Behavior Regulation, Guangzhou , China) Abstract [Objectives] To investigate the extent to which oriental fruit fly oviposition is repelled by Aloe vera L. extracts, and identify the best eluatants for these extracts. [Methods] A. vera volatiles were extracted with methanol, acetone, ethylacetate, dichloromethane and petroleum, and the degree to which these five crude extracts repelled oviposition was compared. The effect of different dosages of the five extracts on oviposition was studied in non-choice and choice tests. The persistence of the repellent effect of each extract was also measured. [Results] The results show that all five crude extracts deterred oviposition but the acetone extract was the most effective. The effect of acetone extract on oviposition was dose dependent, with deterrence increasing with concentration. Oviposition repellence rates were 68.81% (choice test) and % (non-choice test) at a concentration of 10 mg/ml. The repellent activity of the acetone extract sprayed on guava fruit decreased gradually over time. There was no significant difference in the number of oviposition sites between treatment and control groups on the 5 th day of the experiment, nor was there any significant difference in the fecundity of these groups on the 6 th day. [Conclusion] Aloe acetone extracts strongly repelled oviposition by the oriental fruit fly, and could be developed as a natural oviposition repellent. Though the effect of this extract was not very persistent, improved formulation may improve this. Key words Bactrocera dorsalis, Aloe extract, repellent effect, persistent period * Supported projectsgjhz1140 ** First author @qq.com *** Corresponding author zengxn@scau.edu.cn Received Accepted

2 3 : 469 Bactrocera dorsalis Hendel Stephens et al Hou and Xie Champ and Dyte Azadirachta indica A.Juss 0.2% 1% 2% 4% 73%~ 96.7 % Chien et al Lantana camara L. Phyllocnistis citrella 2003 Peganum harmala L. Tribolium castaneum Herbst Salari et al Lavandula angustifolia Mill Leonurus artemisia (Laur.) S.Y.Hu Fallopia multiflora (Thunb.) Harald Lonicera japonica Thunb Sophora flavescens Ait Scutellaria baicalensis Georgi Artemisia argyi Levl. et Vant. Myllocerinus aurolineatus Voss Lindera glauca (Sieb. et Zucc.) Bl Phthorimaea operculella Zeller Aloe vera L. Aloe chinensis Baker Aloe arborescens Mill Aloe ferox Mill 2012 Tribolium castaneum Herbst Rhizopertha dominica Troctes divinatorius Muller Aedes aegypti Subramaniam et al 材料与方法 1.1 材料供试昆虫及饲养 3~4 cm 30 cm 30 cm 30 cm.. = % 80% L.. D= ~ 寄主植物 Psidium guajava L. [ g cm cm]

3 470 Chinese Journal of Applied Entomology 非寄主植物 Aloe vera L 主要仪器与试剂仪器设备 BS210S SyntechN-1000 Rikakikai SHZ-D GM 33A 15 ml SCQ- 2201B FZ 化学试剂及其他材料 1.2 方法芦荟不同溶剂提取物的制备 g ml ml 10 min 10 min 3 10 mg/ml 不同溶剂提取物对桔小实蝇的产卵驱避活性评价 20~ cm 30 cm 30 cm 24 h 10 ml 1 mg/ml 3 24 h 不同浓度的芦荟丙酮提取物对桔小实蝇的产卵驱避活性 mg/ml 10 ml 24 h 芦荟丙酮提取物对桔小实蝇产卵驱避持效性的研究 10 ml 10 mg/ml % 10% h 数据统计与分析选择性试验产卵驱避率 (%) 对照组产卵量 - 处理组产卵量 100, 对照组产卵量处理组产卵量非选择性试验产卵驱避率 (%) 对照组产卵量 - 处理组产卵量 100 对照组产卵量 SPSS Statistics17.0

4 3 : 471 Mean SE Duncan s DMRT t- 2 结果与分析 2.1 不同提取物对桔小实蝇产卵驱避的影响 5 1 mg/ml 1 1 P < % 2.2 不同浓度的芦荟丙酮提取物对桔小实蝇的产卵驱避活性 P < mg/ml 1 mg/ml F=2.80 P=0.17 F=4.00 P=0.12 Plant Aloe Table 1 Solvent type 表 1 5 种提取物对桔小实蝇产卵驱避活性 Repellent effect of five kinds of extracts against the oriental fruit fly Fecundity Spawning holes % Oviposition repellent rate Methanol d c Acetone e d Ethylacetate d c Dichloromethane c b Petroleum b b 8.76 CK a a Duncan s Data followed by different letters in the same row indicate significantly different by Duncan s multiple range test at 0.05 level. The same with Table 2. 表 2 芦荟丙酮提取物对桔小实蝇的产卵驱避活性 ( 非选择性试验 ) Table 2 Oviposition repellent effect of Aloe acetone extract against the oriental fruit fly in non-selection test % mg/ml Spawning Fecundity Oviposition Concentration holes repellent rate CK a a b b c c d d d d mg/ml F=19.89 P= mg/ml 0.1 mg/ml F= P< mg/ml F=41.29 P= mg/ml F= P< mg/ml F=3.57 P= mg/ml 0.1 mg/ml F=37.50 P= mg/ml F= P< mg/ml F= P<0.001

5 472 Chinese Journal of Applied Entomology 54 Table 3 表 3 芦荟丙酮提取物对桔小实蝇的产卵驱避活性 ( 选择性试验 ) Oviposition repellent effect of Aloe acetone extract against the oriental fruit fly in selective test mg/ml Fecundity Spawning holes Concentration Treat CK Treat CK % Oviposition repellent rate ±3.2 * 166.0± * ±4.1 ** 144.3± ** ±5.0 ** 200.0± ** ±4.7 ** 205.7± ** * P <0.05 ** P <0.01 Data followed by * in the same line indicate significant difference at 0.05 level (P < 0.05), while data followed by ** indicate extremely significant difference at 0.01 level (P < 0.01). 3 d 60% 2.3 芦荟丙酮提取物对桔小实蝇产卵驱避持效性的研究 10 mg/ml d 46.0~ F= P< F= P< F= P< F= P< F=82.55 P= F=5.73 P= F=1.61 P= d 5.0~ F= P< F= P< F= P< F=8.47 P= F=7.00 P= F=1.13 P= F=4.50 P= 图 1 桔小实蝇在芦荟丙酮提取物处理不同时间的番石榴上的产卵量 Fig. 1 Fecundity of oriental fruit fly on the guava treated Aloe acetone extract with different time P <0.05 P <0.01 * indicates significant difference at 0.05 level (P < 0.05); ** indicates extremely significant difference at 0.01 level (P < 0.01). The same below. 图 2 桔小实蝇在芦荟丙酮提取物处理不同时间的番石榴上的产卵孔数 Fig. 2 Spawning holes of oriental fruit fly on the guava treated Aloe acetone extract with different time

6 3 : 473 图 3 处理不同时间的芦荟丙酮提取物对桔小实蝇的产卵驱避率 Fig. 3 Oviposition repellent rate of Aloe acetone extract against the oriental fruit fly at different time % % 3 讨论 Tetranychus cinnabarinus 2014 Anopheles gambiae Matasyoh et al mg/ml 68.81% 89.43% 3 d 60% 5 d mg/ml 83.33% Mikania micrantha 9 d Sesbania cannabina 6 d Paederia scandens Dicranopteris pedata Allium tuberosum Duranta repens 4~5 d Malvastrum coromandelianum Ricinus communis 2 d2004 Nilaparvata lugens 80 % 3 d2010 PVA 2007 参考文献 (References) Bian WB, Wang GC, Gong YF, Sun XL, Li YX, Rpellent and anti-feedant activity of 19 plant essential oils against Myllocerinus aurolineatus. Chinese Journal of Applied Entomology, 49(2): [,,,,, , 49(2): ] Champ BR, Dyte CE, FAO global survey of pesticide

7 474 Chinese Journal of Applied Entomology 54 susceptibility of stored grain pests. Fao Plant Protection Bulletin, 25(2): Chien CC, Yaw JD, Ling LC, Hou RH, Deterrent effect of neem seed kernel extract on oviposition of the oriental fruit fly (Diptera: Tephritidae) in Guava. Journal of Economic Entomology, 89(2): Chen JN, Peng Q, He YL, Antibacterial activity of Aloe extract and its relevant research. Acta Medicinae Sinica, 28(4): [,,, , 28(4): ] Chen L, Biological characteristics of Bactrocera dorsalis reared on different citrus fruits and mechanisms of olfaction. Master thesis. Hangzhou: China Jiliang University. [, :.] Cen YJ, Pang XF, Zhou Q, Peng YF, Xu CB, Bioassay on oviposition repellency of non-preferable plant extracts against citrus red mite Panonychus citri. Chinese Journal of Applied Ecology, 15(9): [,,,,, , 15(9): ] Hou BH, Xie RJ, Depth of pupation and survival of the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae), pupae at selected soil moistures. Applied Entomology & Zoology, 41(3): Huang YZ, Lu YH, Li WJ, Repellent effect of the ethanol extracts from twelve herb plants on the adults of Rhizopertha dominica and Tribolium castaneum. Chinese Journal of Applied Entomology, 47(2): [,,, , 47(2): ] Lu YJ, Liu FJ, Study on effect of garlic and Aloe extract against stored grain insects. Grain Storag, 32(3): [,, , 32 (3): ] Matasyoh JC, Wathuta EM, Kariuki ST, Chepkorir R, Kavulani J, Aloe plant extracts as alternative larvicides for mosquito control. African Journal of Biotechnology, 7(7): Man YF, Zhang XM, Xu Y, Xiao C, Effect of Lindera glauca and its volatiles on the oviposition preferences of the potato tuber moth, Phthorimaea operculella. Chinese Journal of Applied Entomology, 53(4): [,,,, , 53(4): ] Stephens AE, Kriticos DJ, Leriche A, The current and future potential geographical distribution of the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae). Bulletin of Entomological Research, 97(4): 369. Salari E, Ahmadi K, Dehyaghobi RZ, Purhematy A, Takalloozadeh HM, Toxic and repellent effecto of harmal (Peganum harmala L.) acetonic extract on several aphids and Tribolium castaneum (Herbst). Chilean Journal of Agricultural Research, 72(1): Subramaniam J, Kovendan K, Mahesh KP, Murugan K, Walton W, Mosquito larvicidal activity of Aloe vera (Family: Liliaceae) leaf extract and bacillus sphaericus, against Chikungunya vector, Aedes aegypti. Saudi Journal of Biological Sciences, 19(4): Wei J, Acaricidal activity of Aloe vera L. leaf extracts against Tetranychus cinnabarinus (Boisduval) (Acari: Tetranychidae) and separation of the bioactivity substance. Master thesis. Chongqing: Southwest University. [, :.] Wan SH, The preparation and properties research of environment-friendly modified pva polymer films loaded with medicine. Master thesis. Wuhan: Huazhong University of Science and Technology. [, :.] Wang T, Xia QL, Chen JB, Lu SM, Research progress on extraction, isolation and bioactivity of active ingredients from Aloes. Food and Fermentation Technology, 48(4): [,,,, , 48(4): 7 11.] Wang YL, Study advance in incidence and trap control of Bactrocera dorsalis (Hendel). Journal of Environmental Entomology, 35(2): [, , 35(2): ] Zhan GH, Fan XH, Gu ZY, Chen YF, Cai P, Yuan WM, Chu CR, Lin YJ, Hu BH, Living habits of orient fruit fly in Taihu area. Plant Diseases and Pests, 1(3): [,,,,,,,,, , 1(3): ] Zhan KR, Zhao SX, Zhu SF, Zhou WC, Wang NW, Study on viability of Bactrocera dorsalis in china. Journal of South China Agricultural University, 27(4): [,,,,, , 27(4): ] Zhong PS, Liang GW, Zeng L, Oviposition deterrent activity of ethanol extracts of the non-preferable plants to the brown planthopper, Nilaparvata lugens. Plant Protection, 36(4): [,,, , 36(4): ] Zhang Q, Acaricidal compounds isolated from Aloe barbadensis Miller leaf and their activity evaluation against Tetranychus cinnabarinus (Boisduval) (Acari: Tetranychidae). Master thesis. Chongqing: Southwest University. [, :.] Zeng XN, Wu ML, Luo S, Effects of plant volatile constituents on oviposition of citrus leafminer (Phyllocnistis citrella Stainton ). Journal of Plant Protection, 30(2): [,,, , 30(2): ]

168 健等木醋对几种小浆果扦插繁殖的影响第1期 the view of the comprehensive rooting quality, spraying wood vinegar can change rooting situation, and the optimal concent

168 健等木醋对几种小浆果扦插繁殖的影响第1期 the view of the comprehensive rooting quality, spraying wood vinegar can change rooting situation, and the optimal concent 第 31 卷第 1 期 2013 年 3 月经济林研究 Nonwood Forest Research Vol. 31 No.1 Mar. 2013 木醋对几种小浆果扦插繁殖的影响健 1,2 杨国亭 1 刘德江 2 (1. 东北林业大学生态研究中心黑龙江哈尔滨 150040 2. 佳木斯大学生命科学学院黑龙江佳木斯 154007) 摘要为了解决小浆果扦插繁殖中生根率及成活率低等问题