Microsoft Word 张志胜二校

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1 7 8 Vol. 7 No Journal of Food Safety and Quality Aug., 2016 张志胜 *, 齐文聪, 韩晴, 陈晨, 戎平 (, ) 摘要 :,,,, :, ;, ;, ;,,, 关键词 : ; ; ; Extraction and application of cod skin collagen ZHANG Zhi-Sheng *, QI Wen-Cong, HAN Qing, CHEN Chen, RONG Ping (College of food science and technology, Agricultural University of Hebei, Baoding , China) ABSTRACT: Cod skin accounts for one tenth of the total weight during the cod processing, which is rich in collagen. Collagen protein has an important function of supporting the body's organs, conservation organizations and the composition of mesenchymal cells. Extracting collagen from cod skin can not only avoid waste of resources, but also reduce environmental pollution. This paper summarized the common methods of extracting collagen from cod skin in recent years: hot water method, alkaline process, salt method and enzymatic method, and the characteristics of each method were analyzed. Extraction by hot water destroyed collagen structure to the extent that reduced the diaphragm function, stability, assist transmission, etc.; since the alkaline extraction produced a racemic mixture, it was less used; extracting by salt was not suitable for mass production by reason of lack of stability of the product; enzymatic extraction cost less with higher efficiency, so it was widely applied. Due to the unique triple helix structure, low immunogenicity, low production cost, mass production and excellent biodegradability, etc., collagen is widely used in the pharmaceutical, food, cosmetics and other areas, which has great prospects for development. KEY WORDS: cod skin; collagen; extraction method; application 1 引言, , [1] 40~50 [2], 10% [3], [4] * 通讯作者 :,, @139.com. *Corresponding author: ZHANG Zhi-Sheng, Professor, College of Food Science and Technology, Agricultural University of Hebei, Baoding , China @139.com

(College of food science and technology, Agricultural University of Hebei, Baoding 071000, China) ABSTRACT: Cod skin accounts for one tenth of the total weight during the cod processing, which is

2 8, : 3229,,,, Sripriya [5] [6] (Gadus),,, (Gadus morhua) (Gadus ogac) (Gadus macrocephalus) [7],, 80%, [8],,, Å, 300 kda [9,10] Y X Y 3 [11],, [12] 27 [13, 14],, Ⅰ Ⅱ Ⅲ,, Ⅱ ; Ⅳ, ; Ⅴ, [15] 1 [16] 2 鳕鱼皮胶原蛋白的提取工艺,,,, Said [17] 60% 2.1 热水法提取胶原蛋白, ~ ~42 [12] [18],, ph, h ph 5.0, 83.75%, SDS-PAGE Ⅰ,,, (gelatin),,,, [19] 2.2 酸法提取胶原蛋白,, [20] (Box-Behnken),,, [16] 表 1 胶原的分子组成类型及组织分布 Table 1 The composition and distribution of collagen molecule [16] Ⅰ [α1(Ⅰ)]2α2 (Ⅰ), 90%, 96% Gly-X-Y α-, 1% [α1 (Ⅰ)]3 Ⅱ [α1 ( Ⅱ)] 3 Ⅲ [α1 ( Ⅲ)] 3 Ⅳ [α1 ( Ⅳ)] 3,,, 10%,, -S-S-,,, 4 3,,

72 h ph 5.0, 83.75%, SDS-PAGE Ⅰ,,, (gelatin),,,, [19] 2.

3 : 0.47 mol/l h 37.92%, 37.36%, 1.49% Kim [21], 碱法提取胶原蛋白,,,,, DL-, D,, D L, [19,22] 2.4 盐法提取胶原蛋白 - (Tris-HCL),,,,,, [23] 2.5 酶法提取胶原蛋白,,,, [24],, (pepsin-solubilized collagen, PSC) [25],, 1%, 1 10, [26], % h 27.53%, SDS-PAGE, α1 113 kda, α2 110 kda 2.6 其他方法提取胶原蛋白,, [27], 2% (w :w) 53 h 0.70 mol/l 1 240( w:v), % [28], 4, 0.5 mol/l, (PSC) 60 h 40 ku/g 1 10(g/mL),, PSC (17.7±0.7)% (acid soluble collagen, ASC), ASC 3 鳕鱼皮胶原蛋白的应用,,, [29] 3.1 在医学领域的应用,, [30],,, [11],, [31],,, [32], B16, : B16,, 200

054% 10.43 h 27.53%, SDS-PAGE, α1 113 kda, α2 110 kda 2.6 其他方法提取胶原蛋白,, [27], 2% (w :w) 53 h 0.70 mol/l 1 240( w:v), 51. 47% [28], 4, 0.

4 8, : 在食品领域的应用 [33], ; ; ; [34], [35] Munasinghe [36] [37],, - -,,,, ; ; 3.3 在化妆品领域的应用,,, [38],,, [39],,,,, 3.4 在其他领域的应用, [40,41], 1%~3%,,, [12] 4 发展前景与展望, [42],,,,,,, 参考文献 [1]. [M]. :, Ministry of Agriculture Fisheries Fishery Authority. China Fishery Statistical Yearbook [M]. Beijing: China Agriculture Press, [2],,,. [J]., 2009, 30(10): Zhuang YL, Lin X, Lin L, et al. Preparation of series of peptides from skin of cod by compound enzyme hydrolysis [J]. Food Res Dev, 2009, 30(10): [3],,,. (Fe-FPH) [J]. :, 2010, 29(2): Wu WP, Deng SG, Xu T, et al. Preparation of Fe-FPH from cod skin and its analysis in nutrition and structure [J]. J Zhejiang Ocean Univ (Nat Sci), 2010, 29(2): [4],,,. [J]., 2012, 33(2): Cai BN, Chen Y, Pan JY, et al. Optimization of preparation process for cod skin collagen eptide-iron ( Ⅱ) chelate viaresponse surface methodology [J]. Food Sci, 2012, 33(2): [5] Sripriya R, Kumar R. A novel enzymatic method for preparation and characterization of collagen film from swim bladder of fish rohu ( Labeo rohita ) [J]. Food Nutr Sci, 2015, 06(15): [6],,. [J]., 2007, (1): Lian XJ, Lu XX, Liu QS. Progress of fish collagen [J]. Meat Res, 2007, (1): [7],,,. [J]. :, 2015, 34(5): Gu BZ, Wang JB, Teng FF et al. Process for preparing cod skin collagen peptide optimization [J]. J Zhejiang Ocean Univ (Nat Sci), 2015, 34(5): [8],. [J]., 2004, 25(2): Fu YF, Shen YX. Discussion about the use of skin collagen [J]. Food Res Dev, 2004, 25(2): [9] Lima CGA, Oliveira RSD, Figueiró SD, et al. DC conductivity and dielectric permittivity of collagen chitosan films [J]. Mater Chem Phys, 2006, 99(2 3): [10] Chi H L, Singla A, Lee Y. Biomedical applications of collagen [J]. Int J Pharm, 2001, 221(2): [11],. [J]., 2011, 33(2): Zhang Y.Xu YA. Research progress of characteristics and application about aquatic collagen [J]. J Fujian Fish, 2011, 33(2): [12],. [J]., 2010, (1):

Beijing: China Agriculture Press, 2015. [2],,,. [J]., 2009, 30(10): 15 18. Zhuang YL, Lin X, Lin L, et al. Preparation of series of peptides from skin of cod by compound enzyme hydrolysis [J].

5 Wang LN, Huang SZ. Research progress about collagen [J]. Meat Res, 2010, (1): [13],,. [J]., 2004, 25(2): Chen JT, Xu Z, Gu QJ. Progress in research and development on collagens [J]. Adv Biomed Eng, 2004, 25(2): [14] Friess W. Collagen--biomaterial for drug delivery [J]. Eu J Pharm Biopharm, 1998, 45(2): [15],,,. [J]., 2010, (1): 7 9. Cao RA, Li H, Li YL, et al. Physiological function property and application of collagen protein [J]. Meat Ind, 2010, (1): 7 9. [16]. [D]. :, Shi LH. Study on preparation and characters of cod skin collagen and collagenous oligopeptide [D]. Wuxi: Jiangnan University, [17] Said MI, Abustam E, Wahab AW, et al. Chemical characteristics of collagen extract from scapula of bali cattle (Os scapula) produced using different extractant [J]. Pakistan J Nutr, 2015, 14(3): [18],,,. [J]., 2011, 30(2): Zhao R, Wang XD, Zu GR et al. Response surface methodology' s analysis on extracting collagen from cod' s skin by hot water extraction [J]. J Dalian Polytech Univ, 2011, 30(2): [19]. [D]. :, Lin L. Studies on preparation of collagen and bioactivities of collagen polypeptide from the skins of aquatic animals[d]. Qingdao: Ocean University of China, [20],,,. [J]., 2015, 36(24): , 377. Shu Y, Ao R, Song J, et al. Optimization of extraction condition of acid-soluble collagen from cod skin by response surface methodology [J]. Sci Technol Food Ind, 2015, 36(24): , 377. [21] Kim JS, Park JW. Characterization of acid-soluble collagen from pacific whiting surimi processing byproducts [J]. J Food Sci, 2004, 69(8): C637 C642. [22]. (Stichopus japonicus) [D]. :, Bi L. Study on physicochemical characters and biological activities of collagen from the body of sea cucumber(stichopus japonicus) [D]. Qingdao: Ocean University of China, [23]. [D]. :, Ren H. Study on preparation of the collagen form the skin of the cod [D]. Baoding: Agricultural University of Hebei, [24],,. [J]., 2010, (3): Guo YH, Liu YR, Li YJ. Progress in the study of collagen and active peptides in fish [J]. China Food Addit, 2010, (3): [25],,,. [J]., 2005, 24(5): Zhao HY, Liang CC, Miao JL, et al. Preparation and composition analysis of cod skin collagen protein [J]. Chin J Mar Drugs, 2005, 24(5): [26],,,. [J]., 2010, (9): Ma L, Wang XD, Cheng H, et al. Optimization of enzymatic extraction conditions of skin collagen from cod (gadus morrhua) by response surface method [J]. China Brew, 2010, (9): [27],,,. [J]., 2014, 28(03): Mao YH, Liu JH, Yang WG, et al. Optimization of extraction conditions of collagen and its characterization from skin of jumbo flying squid ( dosidicus gigas) [J]. J Nucl Agric Sci, 2014, 28(03): [28],,,. [J]., 2015, 36(12): Xiao F, Zhu WX, Kang HB, et al. Orthogonal array design for the optimization of pepsin soluble collagen extraction from cyprinus carpio haematopterus scale [J]. Food Sci, 2015, 36(12): [29],. Ⅰ [J]., 2006, 17(2): Zhang DJ, Wang L. Structure and function of typeⅠcollagen and the situation and prospect of exploratory development [J]. Lett Biotechnol, 2006, 17(2): [30],. [J]., 2005, (3): 32. Wang JY, Dong J. Deep-sea fish skin collagen tissue engineering and preparation methods [J]. R&D Inf, 2005, (3): 32. [31],,,. [J]., 2012, 31(5): Wang ZC, Sun JS, Ni X, et al. Anti-alcoholic gastric ulcer effect of collagen peptide extracted from skin of Walleye pollock [J]. Chin J Mar Drugs, 2012, 31(5): [32],,,. B16 [J]. :, 2006, 36(S2): Dong YT, Li BF, Wang Y, et al. Preparation of collagen polypeptides from cod skin and the effects of collagen polypeptides on B16 cells [J]. Period Ocean Univ China (Nat Sci), 2006, 36(S2): [33] Darmanto YS, Agustini TW, Swastawati F, et al. The effect of fish bone collagens in improving food quality [J]. Int Food Res J, [34] Mariod A A, Adam H F. Review: gelatin, source extraction and industrial applications [J]. Acta Scientiarum Polonorum Technol Aliment, 2013, 12(2): [35] Hashim P, Mohd Ridzwan MS, Bakar J, et al. Collagen in food and beverage industries [J]. Int Food Res J, 2015, 22: 1 8. [36] Munasinghe KA, Schwarz JG, Whittiker M. Utilization of chicken by-products to form collagen films [J]. J Food Process, 2015, 2015: 1 6. [37],,,. [J]. :, 2009, 39(2): Sun LP, Wang DF, Zhang L, et al. Study on preparation of process meaty flavor by maillard reaction of hydrolyzing pollock skin protein [J]. Period Ocean Univ China (Nat Sci), 2009, 39(2): [38],,,. [J]., 2005, 35(6): Wang XC, Ren LF, Jiang TT, et al. Progress in collagen preparation and

[15],,,. [J]., 2010, (1): 7 9. Cao RA, Li H, Li YL, et al. Physiological function property and application of collagen protein [J]. Meat Ind, 2010, (1): 7 9. [16]. [D]. :, 2012. Shi LH.

6 8, : 3233 its applications in cosmetics [J]. China Surfactant Deterg Cosm, 2005, 35(6): [39]. [D]. :, Li X. Study on the water retention and skin care of cod skin collagen peptide [D]. Qingdao: Ocean University of China, [40],. [J]., 2001, 32(1): Feng XL, Xuan XJ. Development and application of hydrolyzed collagen [J]. Zhejiang Chem Ind, 2001, 32(1): [41],,,. [J]., 2005, 24(5): Leng XJ, Wang G, Yan DW, et al. The use of collagen as a partial substitute for fish meal in diets for allogynogenetic crucian Carp [J]. Fish Sci, 2005, 24(5): [42],,,. [J]., 2008, (9): Wang CY, Wang YH, Wu YJ, et al. Application and Prospects of collagen in food [J]. Food Nutr China, 2008, (9): 作者简介 ( 责任编辑 : 姚菲 ) 张志胜, 教授, 主要研究方向为畜产品加工 @139.com

[J]., 2005, 24(5): 31 33. Leng XJ, Wang G, Yan DW, et al. The use of collagen as a partial substitute for fish meal in diets for allogynogenetic crucian Carp [J]. Fish Sci, 2005, 24(5): 31 33.

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Microsoft Word 张志胜 二校

Microsoft Word 张志胜二校