serum acclimation

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1 血清驯化在PRRS防控中的应用 Application of serum acclimation/therapy in controlling and preventing PRRS 回顾和分析 Review and analysis 广西农垦永新伍少钦 Shaoqin Wu 广西农垦永新畜牧集团良圻原种猪场

2 蓝耳病控制与净化 Control and Eliminate PRRS Biosecurity methods 生物安全措施 ( 空气过滤 ) All in all out 全进全出 Gilt acclimation( serum/modified live vaccine/field isolated live vaccine) 后备猪驯化 ( 血清 / 活疫苗 / 田间分离活病毒 ) Herd Closure+ serum therapy(inoculation) 封群 + 驯化 Depopulation(Total or Partial esp. nursey ) 清群 ( 全部 / 部分 ) P1 site /P2 and P2+ site 分胎次饲养

3 提纲 Outline 血清驯化方法 the methods of serum acclimation/therapy 全群血清驯化 ( 血清治疗 ) 与母猪流产 serum therapy and sow abortions 后备猪的血清驯化 serum acclimation in gilt replacement 成功净化场的案例分析 one successful case of eliminating the PRRSV 小结 summary

4 血清驯化方法 Methods of Serum Acclimation/Therapy 血清制备 prepare the serum for acclimating 血清驯化过程 the process of serum acclimation 驯化后指标跟踪 Evaluate the acclimation

5 血清制备 Prepare the serum 寻找 PRRS 疑似猪, 通过群体选择和个体选择确定合适的猪只, 采血并标识, 用 ORF5 引物, 确定相应猪只, 并送测序公司测序, 比对相互间序列差异 Bleed the PRRS-like clinic pigs, get PRRS infection pigs using RT- PCR(ORF-5), get the sequence of the field virus and compare with the old one. 通过荧光定量 PCR 确定病毒载量, 确定驯化用的血清量 Using real-time PCR, make sure the dose of PRRS virus

6 血清驯化过程 Process of Serum Acclimation 血清驯化前 2 天对种猪群进行饲料保健, 采用肌肉注射或喷鼻等方式对猪群进行驯化, 驯化过程中个别不吃料的猪只, 要进行相应的个体治疗 Add some antibiotic before PRRS serum acclimation by I.M. or a nasal spray. Treat some anorexia pigs with antibiotic.

7 驯化后跟踪 Tracing some index after acclimating 驯化后第 2 天, 随机采血 30 头猪 PCR 检测 (5 头 / 管 ) 监测 PRRSV, 评估驯化是否成功 Bleeding after acclimating 2 days, random 30 pigs, detect PRRSV by RT-PCR(5 pigs/pose), evaluate the results 母猪全群驯化, 驯化后跟踪抗原 / 抗体和生产指标变化, 固定 30 头母猪, 每个月监测抗体, 跟踪一年 PRRS 抗体的变化 If acclimating the whole farm, tracing the change of Ag/Ab and production performance, fixed 30 sows, monitor the PRRSV-Ab monthly, lasting more than 1 year.

8 Time 次数血清驯化汇总表 Serum Therapy 事件时间 Date of happening 天数 days SI * 1 date SI2 date AT # start AT peak AT end start peak duration 1 7/14/20077/16/20077/16/20077/20/20077/27/ /30/2013 7/2/2013 6/30/2013 7/6/2013 7/12/ /26/20112/28/20112/26/2011 3/4/2011 3/11/ /7/2007 \ 8/7/2007 8/14/20078/18/ /8/2011 3/10/20113/12/20113/17/20113/21/ /12/2007 \ 9/12/20079/17/20079/23/ /14/2007 \ 9/15/20079/20/20079/23/ /18/20141/20/20141/19/20141/22/20141/24/ /9/2014 4/11/20144/12/20144/15/20144/17/ /3/2015 5/5/2015 5/5/2015 5/9/2015 5/13/ /26/20163/30/20163/26/2016 4/1/2016 4/2/ /30/2016 4/2/2016 3/30/2016 4/6/2016 4/10/ # * AT=abortion Average/ SI=serum inoculation 平均

9 2011 ZXZ sow farm Ab rate: 0.95%

10 ZXZ 繁殖指标 Reproduction Performance May 2015 PRRS unstable Time/ year Sow bred Conception Rate Farroing Rate Born alive Total born Born alive percent % 80.97% % % 82.61% % % 86.05% % % 87.05% % % 89.12% % % 89.28% % % 83.61% % % 89.34% % 2011/3/8 和 3/10 血清驯化, 期间流产数无明显增加活仔由 2011 年 9.96 头上升到头配种分娩率同 86.05% 上升到 89.28%; 由上数据分析血清驯化,PRRS 的稳定期 3 年 Herds inoculated with serum at Mar.8 th and Mar. 10 th. Born alive from 9.96 to 10.58, FR increased from 86.05% to 89.28%; in this farm PRRS stable status 3 years

11 ZXZ PRRS-ORF5 sequence 2015 年 11 月测序的 ORF 5 序列, 与 11 年的相似度只有 96.5% The sequence PRRS ORF-5 of Nov only 96.5% similarity compare to 2011 s.

12 ZXZ PRRS-ORF5 sequence 采用血清驯化的猪场, 猪场内部的 PRRS-ORF5 的序列整体比较接近 The sequence of PRRS ORF-5 is similar when using serum acclimation.

13 2011 HP sow farm Ab rate: 2.87%

14 繁殖指标 Reproduction Performance Time/ year Sow bred Conception Rate Farroing Rate Born alive Total born Born alive percent % 73.85% % % 77.52% % % 81.70% % % 90.32% % % 86.83% % 2011 年 1 月 17 头母猪流产,2 月 9 头流产, 决定全群驯化,2 月 26/28 号 2 次全群血清驯化, 期间 15 头母猪流产,3 月配种分娩率略有下降 ( 由 79.89% 降至 75.50%),2013 年分娩率为 86.83%; 活仔由 9.24 升至 Herds inoculated with serum at Feb.26 th and Feb.28 th. Born alive from 9.24 to 10.17, FR increased from 81.70% to 86.83%; in this farm PRRS stable status 3 years.

15 YZ sow farm 5.56%

16 繁殖指标 Reproduction Performance Time/ year Sow bred Conception Rate Farroing Rate Born alive Total born Born alive percent % 83.74% % % 84.93% % % 86.39% % % 83.26% % % 85.04% % % 87.38% % % 90.49% % % 81.68% % YZ 猪场 2013 年 6 月 30/7-2 两次血清驯化, 分娩率由 13 年的升到 15 年的 90.49%, 胎产总仔由 9.70 上升至年由于 PRRS 不稳定, 又有所下降

17 后备猪血清驯化 Serum Acclimation in Gilts 实施 PRRS 阳性血清驯化后备猪, 可提高母猪群配种分娩率总仔 / 活仔 2009 年对部分场实施 PRRS 血清驯化,2010 年开始, 所有猪场实施, 血清驯化主要针对公斤选留后备种猪从 EZ 猪场生产数据表明 ( 见下表 ), 实施血清驯化是有效的 Utilize serum acclimation for PRRS, can improve sow farrowing rate, total born and born alive. Beginning in one sow farm in 2009, all farms of YX started serum acclimation for gilts in the serum acclimation is very effect as to the data of EZ sow farm.

18 EZ 生产指标 Reproduction Performance Time/ year Sow bred Conception Rate Farroing Rate Born alive Total born Born alive percent % 76.63% % % 80.38% % % 83.36% % % 89.37% % % 86.07% % % 88.84% % % 79.02% % % 88.40% % % 91.16% %

19 成功净化 PRRS 的案例 Successful case of eliminating PRRSV in a sow farm

20 血清驯化过程 Serum Inoculation 时间 Time 说明 comments 全群母猪 + 后备,1101 头基础母 all sows + gilts 驯化期间 (2 周 ) 流产 10 头, 死淘 13 头 abortion 10, cull 13 during serum therapy( in 2 weeks) 血清驯化 + 封群是控制或净化 PRRS 有效的方法 2013 年冬天猪场变得 PRRS 不稳定,2014 年 1 月决定血清驯化 ( 包括 6 个月以上后备猪 ) 和封群 Serum inoculation (therapy) with herd closure is a good method for control or eliminating PRRS in sow farm. The farm had 1200 sows in The status of PRRS became unstable in 2013 winter. According with clinical signs, we decided to inoculate PRRSV serum to sows and gilts, and then closed the herd.

21 Sow abortion of acclimation 驯化前后流产情况

22 母猪跟踪检测 ELISA Ab( IDEXX) Sow

23 公猪跟踪检测 ELISA Ab( IDEXX) Boar

24 公猪跟踪检测 ELISA Ab( IDEXX) Boar

每年常规检测 ELISA PRRS-Ab (IDEXX) 2014 PRRS boar sow gilt piglet nursey finish sample 266 471 75 52 264 530 >0.4 60% 52% 45% 19% 14% 13% >2.5 3% 2% 0% 2% 0% 0% >1.5 14% 10% 5% 4% 2% 8% Avg 0.71 0.62 0.

25 每年常规检测 ELISA PRRS-Ab (IDEXX) 2014 PRRS boar sow gilt piglet nursey finish sample >0.4 60% 52% 45% 19% 14% 13% >2.5 3% 2% 0% 2% 0% 0% >1.5 14% 10% 5% 4% 2% 8% Avg CV Std PRRS boar sow gilt piglet nursey finish sample ,002 >0.4 3% 13% 1% 1% 0% 0% >2.5 0% 0% 0% 0% 0% 0% >1.5 0% 0% 0% 0% 0% 0% Avg CV Std PRRS boar sow gilt piglet nursey finish sample >0.4 2% 24% 1% 5% 0% 0% >2.5 0% 0% 0% 0% 0% 0% >1.5 0% 1% 0% 0% 0% 0% Avg CV Std

26 母猪场 PRRS 抗体检测结果 ( ) 项目年份公猪母猪后备猪仔猪保育育肥样本 % 52% 45% 19% 14% 13% s/p> % 24% 1% 5% 0% 0% 阳性 % 12% 1% 0% 0% 0% % 5% 1% 0% 0% 2% s/p 平均值

27 生产数据 Reproduction data Time/ year Sow bred Conception Rate Farroing Rate Born alive Total born Born alive percent % 90.82% % % 84.26% % % 89.14% % % 90.07% % % 89.66% %

28 Farm code 血清驯化小结 Serum therapy summary Date of happening days SI * 1 date SI * 2 date AT # start AT # peak AT # end start peak duration F1 7/14/2007 7/16/2007 7/16/2007 7/20/2007 7/27/ F1 6/30/2013 7/2/2013 6/30/2013 7/6/2013 7/12/ F5 2/26/2011 2/28/2011 2/26/2011 3/4/2011 3/11/ F4 8/7/2007 \ 8/7/2007 8/14/2007 8/18/ F4 3/8/2011 3/10/2011 3/12/2011 3/17/2011 3/21/ F3 9/12/2007 \ 9/12/2007 9/17/2007 9/23/ F2 9/14/2007 \ 9/15/2007 9/20/2007 9/23/ F8 1/18/2014 1/20/2014 1/19/2014 1/22/2014 1/24/ F6 4/9/2014 4/11/2014 4/12/2014 4/15/2014 4/17/ F4 5/3/2015 5/5/2015 5/5/2015 5/9/2015 5/13/ F6 3/26/2016 3/30/2016 3/26/2016 4/1/2016 4/2/ F7 3/30/2016 4/2/2016 3/30/2016 4/6/2016 4/10/ # AT=abortion * SI=serum inoculation average

29 结论 Summary 后备猪的血清驯化值得去做, 尤其不免疫蓝耳苗的猪场 ; Serum acclimation useful tool for gilts, esp. for Non-vaccine farm 整个母猪群血清驯化, 可尝试尤其是疫苗无效时, 但有风险 ; Serum inoculation (therapy) also is a tool, esp. no effect with vaccine, but it has risk. 血清驯化 + 封群 + 一次引够后备, 加强生物安全, 可做到 PRRS 净化 Serum therapy + herd closure + 6 months gilt(acclimated the same time) + strengthen farm biosecurity >>> eliminate PRRS

30 推广价值 Extension value 2007 年良圻原种猪场开始在母猪群中应用血清驯化控制蓝耳病, 2009 年开始在后备猪中用血清驯化稳定蓝耳病在国内养猪企业中, 良圻原种猪场是最早应用血清驯化控制 PRRS 的猪场之一通过 11 年的血清驯化工作, 加上兽医技术中心的监测 ( 抗体检测, 抗原检测测序和序列分析荣光定量 PCR 等 ), 良圻原种猪在蓝耳病控制和净化方面积累了大量的第一手资料 Liangqi Farm applied serum therapy to control PRRS in sows during 2007, applied with serum acclimation in gilt during Liangqi Farm is one of the first farms applied serum acclimation for control PRRS. We has lots of experiences in controlling and eliminating PRRS

31 推广价值 Extension value 血清驯化的时机非常关键, 血清驯化不一定能保证 100% 成功, 其中有一些不可预料因素, 是否决定全群血清驯化, 有时是一个艰难的抉择 ; 但是只要充分了解本场的健康状况, 同时加上周密计划, 血清驯化的成功率还是很高的良圻原种猪场在 PRRS 控制与净化方面的多年实践, 为国内蓝耳病的控制与净化提供了宝贵的经验, 值得国内外的养猪同行参考与借鉴 The timing for serum acclimation is very critical, we can t guarantee all successful when apply serum acclimation/therapy, many uncertain factors. Sometime it is a dilemma to do or not do serum acclimation. But if we know much more the farm and prepare in advance, we can get good results from it. What we did in past 11yeats provide some important references for swine producers.

32 致谢 Acknowledgment 吴志君总经理 Mr. Zhijun Wu 闫之春博士 Dr. Jason Yan 朱汉守教授 Dr. HanSoo Joo 莫里森教授 Dr. Bob Morrision 谢体三博士 Dr. Tishan Xie 曾容愚博士 Dr. Rongyu Zeng 马玲博士 Dr. Ling Ma 良圻兽医技术中心 Vet diagnose Lab of Yongxin Husbandry company 农垦永新良圻全体人员 Employees of State Farms Yongxin Husbandry company

33 Critiques welcome!

PowerPoint Presentation

PowerPoint Presentation An Update on the Most Costly Pig Disease PRRS 最昂贵猪病 - 蓝耳病的最新情况 S Professor Morrison DVM, MBA, PhD 莫教授 College of Veterinary Medicine University of Minnesota Outline 提纲 We re not decreasing incidence. 我们没有降低蓝耳病发生率