33 2 2014 4 CHINESE JOURNAL OF MARINE DRUGS Vol.33 No.2 April2014 * 1 1* 1 2 1 1 3 3 (1. 266021;2. 266021; 3. 116100) : 本文旨在探讨海参精囊提取物对环磷酰胺导致的雄激素部分缺乏 生殖系统受损小鼠的保护 作用 昆明种小白鼠随机分组 除正常组用生理盐水外其他各组均腹腔注射环磷酰胺 (CP28mg/kg) 每天 1 次 连续 5d 复制生殖系统受损 雄激素部分缺乏模型 ; 同时治疗组每天灌胃不同剂量的药物 1 次 连 续 4 周 记录小鼠的体重并观察其精神状态计算脏器指数 检测血清中睾酮含量 观察睾丸组织切片中生精 上皮的厚度和生精细胞的数量 并与模型组进行比较 相比于模型组小鼠 虽海参精囊提取物高低剂量 间无明显差异 (P>0.05) 但治疗组小鼠的脏器指数 血清睾酮含量 生精上皮厚度和生精细胞数量均有明显 的改善 (P<0.01) 甚至接近或高于正常水平 海参精囊提取物能显著提高环磷酰胺导致的生殖系统受 损小鼠的血清睾酮含量 并且对损伤的生殖系统具有一定的保护作用 : 海参精囊 ; 环磷酰胺 ; 雄激素部分缺乏 ; 生精细胞 :R965 :A :1002-3461(2014)02-051-06 DOI:10.13400/j.cnki.cjmd.2014.02.009 Effectsofholothurianspermatophoreextractsonmicegenital systeminjuredbycyclophosphamide GUO Xi-chun 1 GAO Hua 1* LIU Kun 1 LIU Xiao-ping 2 LIUZhan-tao 1 ZHANGJie 1 LIU Chun-bao 3 WU Yan-qiang 3 (1.Schoolof PharmaceuticalScienceQingdao UniversityQingdao266021China; 2.Schoolof MedicineQingdao UniversityQingdao266021China; 3.Dalian BangchuiIsland Seafood Co.LtdDalian116100China) Abstract:ObjectiveToinvestigatetheprotectiveefectsofholothurianspermatophoreextractsonpar- tialandrogendeficiencyofthe micegenitalsysteminjuredbycyclophosphamide(cp).methods Male Kunmingmicewereequalyalotedtogroupsandthey wereintraperitonealyinjected with CP (28 mg/kg)onceadayfor5daystoconstructinjuredgenitalsystemandpartialandrogendeficiencymodel exceptthattheregulargroupwasintraperitonealyinjectedwithnormalsaline.miceinthetreatment groupsoralyreceivedextractsonceadayfor4weeks.thechangesofbodyweightandspiritstatewere observed.visceralindextestosteroneconcentrationinserumthicknessofspermatogenicepithelium andspermatogeniccelsamount weredeterminedandcompared withthoseof modelgroup.results Comparedwiththatinmodelgroupthoughtherewasnoobviousdiferencebetweenthedosesofholo- * : (20123702002525) : (1988-) : E-mail:gxch3803@163.com * : Tel:0532-82991203E-mail:gaohuaqy@126.com :2013-09-25
52 33 thurianspermatophoreextracts(p>0.05)thevisceralindextestosteronelevelsinserumthickness ofspermatogenicepitheliumandnumberofspermatogeniccelswerealsignificantlyimproved (P< 0.01)evenclosertoorhigherthanthenormallevel.Conclusion Holothurianspermatophoreextracts canimprovetestosteronelevelsinserumcontentofthemicewithdamagedgenitalsystemcausedbycp andhaveaprotectiveefectonthedamagedgenitalsystem. Keywords:holothurianspermatophore;cyclophosphamide;partialandrogendeficiency;spermatogenic cels (PAD- :12031425) AM) AR5120 ( ( ) (T) );DK-98-1 ( [1] 1 );SK3200H ( ); ( [2] ); B1 (TST) ( );LD4-2A ( );DFM-96 ( [3-4] ); ( ) 1.3 样品制备 : 1 3 [5] 30 25min 30 2h 4000r/min 5 min 1 2 0.09 MPa 1 1.1 实验动物与原料 96 (25~35)g SPF 1 5 :SLXK 20080002 : 1 10 30 3h; 1.4 动物分组及模型 96 8 1.2 试剂与仪器设备 0.9% ( 28 mg kg -1 d -1 5d :1207215121); (5 mg/ :110802); 5 ( : (300 600mg kg -1 d -1 ) 130220); (0.2g/ (300 600mg kg -1 d -1 )
2 : 53 (600mg kg -1 d -1 ) 1.5mg kg -1 d -1 1 4 1.5 指标检测 1.6.4-80 珡 X±S SPSS17.0 ; 10% LSD 24h 1.6 观察指标及方法 1.6.1 2.1 一般状态观察 ; = / 100% 1.6.2 1.6.3 SNK 2 HE ( 1) 1 2.2 海参精囊提取物对小鼠脏器指数的影响 ( 见表 1) Fig.1 Thesurvivalcurveofmice (P<0.05); (P<0.01) (P<0.01) 2.3 海参精囊提取物对小鼠血清睾酮含量的影响 ( 见表 1) (P<0.01)
54 33 1 (n=10 X±s) 珡 Table1 Efectsofholothurianspermatophoreextractsonvisceralindexandtestosterone concentrationinserum(n=10 X±s) 珡 /Group (SI) /% (TI) /% /Serum testosteroneconcentration)/pg ml -1 4.1617±0.8005 1.2917±0.1011 158.50±14.79 3.2650±0.4460 0.9367±0.1879 67.67±19.31 5.4683±0.7853 1.2317±0.1825 139.50±12.57 5.3700±0.6206 1.1200±0.1420 146.67±28.32 5.7517±0.8519 1.3083±0.0995 159.17±29.29 5.8433±0.9470 1.5000±0.1702 147.33±17.59 6.0250±0.9137 1.4133±0.1490 416.17±47.38 8.8633±0.4274 1.5650±0.1364 512.83±59.60 : P<0.05 P<0.01 ; P<0.01 Note: P<0.05 P<0.01vscontrolgroup; P<0.01vsmodelgroup. 2.4 海参精囊提取物对睾丸组织形态的影响 2.4.1 ( 22) ( 23~7) 2 ( H.E. 100) Fig.2 Thehistologicalstructuresinthetestisofmice(parafinsectionH.E. 100) 1 ;2 ;3~4 ;5~6 ;7 ;8 1Controlgroup;2Modelgroup;3~4Lowandhighdosesofwaterextractsgroup;5 ~ 6Lowandhighdosesofalcoholextracts group;7freeze-driedpowdergroup;8positivedruggroup. 2.4.2 (P<0.01); ( 2) 0.01) (P<
2 : 55 2 (n=10 X±s) 珡 Table2 Efectsofholothurianspermatophoreextractsonthethicknessofspermatogenicepithelium andspermatogeniccelsamountofmice(n=10 X±s) 珡 /Group /Spermatogenic epitheliumthickness/mm /Spermatogenic celsamount/mm -2 0.8306±0.0534 3.6888±0.0814 0.4611±0.0218 2.2028±0.0772 0.6555±0.0164 3.1697±0.1288 0.6621±0.0306 3.2360±0.1157 0.6426±0.0236 3.0195±0.1178 0.6908±0.0290 3.1500±0.0458 0.7307±0.0194 3.2750±0.0875 0.7547±0.0190 3.4473±0.0890 : P<0.01 ; P<0.01 Note: P<0.01vscontrolgroup; P<0.01vsmodelgroup. 3 (cyclophosphamidecp) [6] ; DNA [7] [9] CP :(1)CP CP CP c-kit p53 Fas/FasL (2) [8] CP A CP CP (SCFm) Ser- toli A (3)CP PADAM TST Sertoli (4)CP 3β-HSD 17β-HSD TST
56 33 [2] TST CP 1932-1933. CP [4] [10] [5] [6] CP CP PADAM [J]. 20126(15):4397-4399. [1]. [J]. 200410(8):563-566.. [J]. 200813(3):157-159. [3]. [J]. 200622(16):. -1 [J]. : 200728(3S):134-135.. [J]. 200931(8):1263-1269.. [J]. 200921(4):42-44. [7] EmersonREUlbrightT M.Intratubulargermcelneopla- siaofthetestisanditsassociatedcancers:theuseofnovelbi- omarkers.[j].pathology201042(4):344-355. [8] Yan WSouminenJToppariJ.Stem celfactorprotects germcelsfromapoptosisinvitro[j].j CelSci2000113 (6):161-168. [9]. [J]. 200626(10):1389-1391. [10].