3 3 ( ) Vol.3No.3 200$9 JournalofYangzhouUniversity(AgriculturalandLifeScienceEdition) Sep.200 犈 2 犘 4,,, 2, 2,, (. /, 24062;2.!", 24062)! ":#$%&' 3 (A B C) ( ) * +, 72 -.,/ 0 2! 3 4 5 6 7 8 9 (TE2) :;(P4), <=>?@AB CDE : ( F )B C 45 P4 G HIJ 2 ( F K$ )( 犘 <0.05);2 A 45 P4 IJ C ( 犘 <0.05); 45 P4 IJ 2 ( 犘 =0.00),LMN @ TE2 O ( 犘 >0.05) A B C @ -., 45 TE2 @PQR HS68 80 80pg ml -,P4 HS.76.85.38ng ml - ; 2 4 5 TE2 @PQR HS80 60 60pg ml -,P4 H S.55 0.95 0.76ng ml - T U -. P 4 5 TE2 P4 @PQR HS76pg ml -.57ng ml -,2 HS64pg ml -.3ng ml - #$TU -.45 TE2 P4 @PQR HS72pg ml -.46ng ml - E ()*+V WX 45 P4 Y O, F I;-. 45 TE2 UN Z #$:*+;789;:;; 2;<= %&' :Q579.;TS252.2 (: )* :67 4652(200)03 0025 05 犜犺犲狉犲犾犪狋犻狅狀狊犺犻狆犫犲狋狑犲犲狀狋犺犲犮狅狀犮犲狀狋狉犪狋犻狅狀狊狅犳犲狊狋狉犪犱犻狅犾犪狀犱狆狉狅犵犲狊狋犲狉狅狀犲犻狀犮狅犿犿犲狉犮犻犪犾狑犺狅犾犲犿犻犾犽犪狀犱狊犲犪狊狅狀狊犪犾狋犲狉狀犪狋犻狅狀 犕犃犢犪 狆犻狀犵, 犡犝犣犺狌犪狀犵 犼犻犪狀, 犠犃犖犌犙犻狀犵, 犎犝犢狌 2, 犣犎犃犗犑犻犪狀 狑犲犻 2, 犣犎犃犖犌犠犲狀 犼犻犲, 犠犃犖犌犡犻 狆犻狀犵 (. 犜犺犲 4 狋犺犆犾犻狀犆狅犾, 犛狅狅犮犺狅狑犝狀犻狏 / 犇犲狆狋狅犳犘犲犱, 犠狌狓犻 4 狋犺犘犲狅犎狅狊狆,Wuxi24062,China; 2. 犇犲狆狋狅犳犈狀犱狅犔犪犫, 犠狌狓犻 4 狋犺犘犲狅犎狅狊狆,Wuxi24062,China) 犃犅犛犜犚犃犆犜 :Seventytwosamplesfromthreebrands(A,BandC)ofcommercialwholemilkwereinvestigatedinayear. Asampleinalotnumberwascolectedfromeverybrandonceeveryhalfamonth.Totalestradiol(TE2)andprogesterone (P4)inwheywereassayedbyimmunochemiluminometricassaystoobservetherelationshipbetweenTE2 orp4incom mercialwholemilkandseasonsalternation.theresultsshowedthat:p4concentrationsinbrandbandcatthefirststage (fromspringequinoxtoearlyautumn)wererespectivelyhigherthanthoseatthesecondstage (fromearlyautumnto SpringEquinoxinthenextyear)( 犘 <0.05inboth).Atthesecondstage,P4concentrationinthebrandA washigher thanthatinthebrandc ( 犘 <0.05).P4concentrationforthealofthelotnumbersof3brandsatthefirststagewashigh erthanthatatthesecondstage( 犘 =0.00),andnostatisticalysignificantdiferenceinTE2 wasfoundbetweenthetwo stages.atthefirststage,medianforte2amonglotnumbersaboutbranda,bandc wererespectively68,80and 80pg ml -,andforp4 wererespectively.76,.85and.38ng ml -.Atthesecondstage,medianforTE2 amonglotnumbersaboutbranda,bandcwererespectively80,60and60pg ml -,andforp4 wererespectively.55,0.95 and0.76 ng ml -. Medianfor TE2 and P4 inallotnumbersatthefirststage wererespectively 76pg ml - and.57ng ml -,andatthesecondstagewererespectively64pg ml - and.3ng ml -.Me dianforte2andp4inallotnumbersinayearwererespectively72pg ml - and.46ng ml -.Itwasconcluded thatthereweresignificantdiferencesinp4 ofmilkwheyamongthesamebrandsaswelasdiferentbrands,andp4 of milkwheyfromspringequinoxtoearlyautumnwashigherthanthatinotherperiodinayear,andtherewasalsofluctu ationinte2ofmilkwheyamonglotnumbers. 犓犈犢犠犗犚犇犛 :milk;estradiol;progesterone;immunochemiluminometricassays;seasons +,-.:2009 05 27 /0 : [\]^_`a(2007 2) :bc (980- ),,,, B,E mail:xxx959xzj@26.com
26 J c( 8 ) 32!"#$%& [] ' [2] ()6* +,-. "/0# (E 2 ) (P 4 ) 234,5 4*+"#%&!67, 2*89:; ;E 2 7 "4.8,P 4 7 "2.5 <= [,3] 5 ">?8@AB%&CD$E "5 "CDD () "FG$HI C D<=JKL MN O [4] [5] [6] & P [7] QRSTU,D #%&VWX THISY, #%&!" < Z[\,]8^_"CD$`abc '<=G Q L3*+,-. 72*, 5 (ICMA)34 # (TE 2 ) P 4!, M<=]8^_ "D, #%&FG$<= ( ) Sigma );ACCESSG N 5 ( ) Beck man ) 2 W, -"' "., 3*(A B C)+, 2007 3 6 ~2008 3 5, + L*,G 72 3!"# ICMA 34,-. TE 2 P 4!,34 8% M/0 2 " E 2 ] / 0 T ' TE 2! " 3 4 [8] T : 2mL,2000r min - 0 90min, ;34P 4 ; 0.25mL, 0.7mL 0.05mL ( 50U), 37 2h,3 4 E 2 TE 2 = 4 /0.25 4 $% &' G 2*,( ) 2007 3 6 ~8 3,2( ) 2007 9 ~2008 3 5 +*, 33* ;2 +3*, 39* 5 SPSS3.0 : $3 (Shapiro wilk ) T $3 (Levene ) T $" Z,K!6" #$&T, %%!6 Student Newman Keuls,&% " '!6 ' ' 狋 3 ; T," 3,K ' "!6 Kruskal WalisH 3,] ' "%%!6 Nemenyi,&% ' "!6 Mann WhitneyU 3 2 ( 2 )$ 2*&'+,-./ 0 ( b,)^ + + ^_ " TE 2 P 4 W * 3*+ TE 2 P 4! + (7.2 ± 39.2)pg ml - (.380 ± 0.588)ng ml - + + " TE 2 *,-., P 4 8 TE 2,M +, B C + P 4 7 ]2, 犘 + 0.032 0.06;2 A + P 4 7 C +;]/ + + P 4 *,- 2,3*+ TE 2 + (75.5 ± 37.6) (67.5 ± 40.5)pg ml -,% W,- *;3*+ P 4 + (.62 ± 0.548) (.75 ± 0.546)ng ml -, 0,- 7 0( 犘 <0.0)
3 Q:,-. E2 P4!8^_ "D 27 )$ 2*&'+,-./ 0 犜犪犫. 犜犺犲犮狅狀犮犲狀狋狉犪狋犻狅狀狊狅犳犳犲犿犪犾犲狊犲狓犺狅狉犿狅狀犲狊犻狀狑犺犲狔犳狅狉狋犺犲犫狉犪狀犱狊狊狅犾犱犻狀狋犺犲犮犻狋狔犻狀犳狅狌狉狊犲犪狊狅狀狊犪狀犱狋狑狅狊狋犪犵犲狊 ^_ 狀 #$%& A + B+ C+ ^ 6 TE2!/pg ml - 86.0 ± 7. 59.3 ± 29.2 69.3 ± 36.6 P4!/ng ml -.387 ± 0.539.657±.05 0.95 ± 0.44 3^ 6 TE2!/pg ml - 63.3 ± 35.5 89.3± 35.5 63.3 ± 4.6 P4!/ng ml -.707 ± 0.33.65 ± 0.569.225 ± 0.420 ^ 6 TE2!/pg ml - 72.0± 50.6 63.3 ± 39.3 70.7 ± 5.5 P4!/ng ml -.657 ± 0.97.343 ± 0.527.58 ± 0.584 4^ 6 TE2!/pg ml - 83.3 ± 45.9 67.3± 45.6 66.7 ± 53.0 P4!/ng ml -.558 ± 0.242.25 ± 0.842 0.758 ± 0.250 TE2!/pg ml - 77.8 ± 37.6 8.8 ± 30.0 66.9 ± 45.6 P4!/ng ml -.70± 0.359.807 ± 0.690.355 ± 0.483 2 3 TE2!/pg ml - 74.8 ± 39.7 59.7 ± 40.8 68.0 ± 42.8 P4!/ng ml -.472 ± 0.35.62 ± 0.686 0.892 ± 0.439 82 B+C! *,-( 犘 <0.05); 82 C+C! *,-( 犘 <0.05); 82 C+C! *,-( 犘 <0.05) 2 2 2*&'+,-./ 0 2345 2*,-+. #%&!5 67b(2 ):A B C + ", TE 2 " 8 + 68 80 80pg ml -, P 4 +.76.85.38ng ml - ; TE 2 P 4 " 8 + 76pg ml -.57ng ml - +!65,C + TE 2 5 67 J, J "2.6,& B + P 4 5 J, J "5.33 +!65,A B + TE 2 J 7( 252pg ml - ), C + (92pg ml - )"2.74,& P 4 9 B+5 J 2)2 :A B C + ", TE 2 " 8 + 80 60 60pg ml -,P 4 +.55 0.95 0.76ng ml - ; TE 2 P 4 " 8 + 64pg ml -.3ng ml - + TE 2 P 4 5 J" B+, J 8! + 2 2*&'+,-./ 0 2345 犜犪犫.2 犜犺犲狉犪狀犵犲狊狅犳犳犲犿犪犾犲狊犲狓犺狅狉犿狅狀犲狊犻狀狑犺犲狔犻狀犮狅犿犿犲狉犮犻犪犾狑犺狅犾犲犿犻犾犽犻狀狋犺犲犮犻狋狔犳狅狉狋犺犲犫狉犪狀犱狊犻狀狋狑狅狊狋犪犵犲狊 狀 + TE2!/ P4!/ pg ml - ng ml - A 24 ~ 252.03 ~ 2.4 B 36 ~ 252 0.55 ~ 2.93 C 92 ~ 240 0.64 ~ 2.05 2 3 A 08 ~ 260 0.67 ~.98 B 92 ~ 236 0.42 ~ 2.37 C 20 ~ 268 0.38 ~ 2.2 2.57 5.64 +,C + TE 2 " J 7(268pg ml - ), B+ "2.9,& B+ P 4 " J 7(2.37ng ml - ), C + "6.24 3)G,-+. TE 2 P 4! :;:G TE 2 P 4 " 8 + 72 pg ml -.46 ng ml -,] + (7.2±39.2)pg ml - (.380± 0.588)ng ml - b,g,-+. P 4!7 2,& TE 2!% W * 3 67 %&",D<=$%&8>?D " 0 @AK B>?",-.#%& C6]00 9:(D )E,A7 ] $ T [3] [9], 8 FD GHMI*J KLM NA C,O] F, "#%& A [] J 75%",-D= F [8]
28 J c( 8 ) 32 8 9:+,-./ 0 犜犈 2 犘 4 ; 犉犻犵. 犜犺犲犮犺犪狀犵犲狊狅犳狋狅狋犪犾犲狊狋狉犪犱犻狅犾犪狀犱狆狉狅犵犲狊狋犲狉狅狀犲犻狀犿犻犾犽狑犺犲狔犳狉狅犿犕犪狉 6 狋犺,2007 狋狅犕犪狉 5 狋犺,2008 FaustiniQ [0] c "PQRJ " ( S )P 4 TQ 0.259ng ml - '<= ICMA 34"72* P 4 " + 0.38.38ng ml -, + P 4 W,- *,U34T,.V W XY4, P 4 C M F67 # TE 2 #! Z 7 2 F 4~60 [ # 5pg ml - [8] Y4, F4~60[ TE 2 43pg ml -,M <=" TE 2 + 92 7pg ml -,\` ]] C M F67,$^ ' <"+ G F,_K WJ 75% ", - D = F " ` a '<= TE 2! "5, 8 ' F!Z ] b F D '<= X(E,,-.+ + P 4 M * c C ^_,$^,^ J b F '<=( ) P 4 7 2, 8^ JK b F D;2( )A + P 4 7 C +, 8 + F D,#%& 8 %& Q M [ 2],+ P 4 * 8^$&D,-.8>?"<= b [,3] TU"c,D] O"<=6\, M GanmaaQ [8] <=Va5 J E,",. Z,3Z * Q [9,3] <=`a5 #%& O # J E," ' <=,], F N F ",-. # $ 24h # P 4 AK [4 5] ; $ "5 ", 5 T 4! [6] ; $ [7] 7#$ 7! [8],-. "#%& W V CD O <= <: [] GanmaaD,SatoA.Thepossibleroleoffemalesexhormonesin milkfrom pregnantcowsinthedevelopmentof breast,ovarianandcorpusutericancers[j].medhypotheses,2005,65(6):028 037. [2],,,Q. # " C [J]. ),2007,9(2):39 4. [3] QinLQ,WangPY,KanekoT,etal.Estrogen:oneoftheriskfactorsinmilkforprostatecancer[J].MedHy potheses,2004,62():33 42. [4], F,,Q. PCR 34# A "<= [J]. J c: 8,2003,24(3): 4. [5],, 7. >? " [J].,2007,28(7):47 49. [6],,,Q.C 0 = 9 P [J]. 4 c,2007,26(3):33 334. [7],.! 34 & P"<= [J]. ) ",2008,36(3):5 53.
3 Q:,-. E2 P4!8^_ "D 29 [8] GanmaaD,QinLQ,WangPY,etal.Atwo generationreproductionstudytoassesstheefectsofcows milkon reproductivedevelopmentinmaleandfemalerats[j].fertilsteril,2004,82(suppl3):06 4. [9],#$%. #$%& O $J " [J]. &I,2003,7(2):97 00. [0] FaustiniM,Batocchio M,VigoD,etal.Pregnancydiagnosisindairycowsbywheyprogesteroneanalysis:an ROCapproach [J].Theriogenology,2007,67(8):386 392. [] BalS,Polson K,EmenyJ,etal.Inducedlactationinprepubertalholsteinheifers [J].JDairySci,2000, 83():2459 2463. [2],',#,Q. M @" M [J]. ()*+,2008(6):29 30. [3],, -,. / 0,Q. # % & O J " [J]. & I,2003, 7(4):27 29. [4],,,Q. F #$ # [J]. ),2007,23(0): 225 227. [5],,,Q. #$%& O # $ # " [J]. 2 +, 2007,34(23):4498 450. [6],,,Q. F $ M 3 4 5 " [J].&I,2009, 23(5):395 397. [7] 567,,,Q. # $ % & $ 3 " [J].8 9,2009, 24(20):60 603. [8] 567. F #$ SD 3" [D].: :: J,2009. (; ) (9) ` b < = 7 > J? $ @AB\,? C 7 D B\, E X ( E, < = L 4 J ] F G H, < = 7 HDL C! I JK?L 3 _ 2 H MNO? $Q (E, < = @ 2 AS",C &,D= P Q6 RS &T UN FG 8 7 < = 7 J ",-, V <=] WX Y "2 ] <: [] J. : ) H " Z[ [J]. \],2006,2(2):77 8. [2] $^. WX Y <= _ [J]. ) + `,2008,5(5):394 396. [3] a. <"<= _ [J].) + : + b,2004,26(6):335 338. [4] c,,. -,Q. < " @ <= [J].,2007,38(0):455 457. [5],,.,Q. ) J " <= [J]. J c: 8,2005,26(2):5 7. [6],,.C "<= [J]. J c: 8,2009,30():22 25. [7],. 7 > " < = [J]. J c: 8, 2009,30(3):64 66. [8],!.7 M W X Y 5 " [J].+ 8 :,2008, 29(7):46 48. [9] a, J,.,Q. < O E " < = [J]. ),2006,8(3): 54 56. [0] JuliusS,JamersonK,MejiaA,etal.Theassociationofborderlinehypertensionwithtargetorganchangesand highercoronaryrisk:tecumsehbloodpressurestudy[j].jama,990,264:354 358. (; )