% & ' 犑狅狌狉狀犪犾狅犳犘犪狋犺狅犵犲狀犅犻狅犾狅犵狔 Feb.2019, Vol.14,No DOI: /j.cjpb HQR)OST + 1,2,c 2,3,c 1,3, 1,3,! " 1,3 (1. /,-+, / V 8

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1 165 DOI: /j.cjpb HQR)OST + 1,2,c 2,3,c 1,3, 1,3,! " 1,3 (1. /,-+, / V ;2. /,-+ %&';3. / ( #$%&') C DE" X NM NCBI EmTspanFG?X H I, 2 ProtParam ProtScale SOSUI DNASTAR SignalP Cel PLoc SOMPA NetPhos MotifScan Phyre2 TMHMM E FGI?H BI Y6[ M X D ; M$ N 2 ABCpred IEDB SYFPEITHI B 6 [ T 6 [? N M,M MEGA X 56@ Tspan? Q EmTspanFG 236 X HI, ^S C1163H1853N291 O315S21,] S 32.77,S F G; 4 X, M 6 [ ;N c 46.19%, β 20.34%, β 8.05%, 25.42%;EmTspanFG V? B 6 [ TCL 6 [ Th6 [? N M S ; Q 7 DE "? TspanD \ P '? TspanO a EmTspanFG,- -? B6[ T 6 [ N M, B, S D E "? ( U V A H > DE"; X FG; 34; NM R383.33!"#$ A %& (2019) [.2019Feb;14(2): ,177.] 犅犻狅犻狀犳狅狉犿犪狋犻犮犪狀犪犾狔狊犻狊狅犳狋犲狋狉犪狊狆犪狀犻狀狊犳狉狅犿犈犮犺犻狀狅犮狅犮犮狌狊犿狌犾狋犻犾狅犮狌犾犪狉犻狊 YANG Yu 1,2,ZHANG Yao gang 2,3,ZHANGLing qiang 1,3,REN Li 1,3,FAN Hai ning 1,3 (1. 犙犻狀犵 犺犪犻犝狀犻狏犲狉狊犻狋狔犎狅狊狆犻狋犪犾, 犡犻狀犻狀犵, 犆犺犻狀犪 ;2. 犔犪犫狅狉犪狋狅狉狔, 犙犻狀犵犺犪犻犝狀犻狏犲狉狊犻狋狔犎狅狊狆犻狋犪犾 ;3. 犓犲狔犔犪犫狅狉犪狋狅狉狔狅犳犈犮犺犻狀狅犮狅犮犮狌狊犻狀犙犻狀犵犺犪犻犘狉狅狏犻狀犮犲 ) 犃犫狊狋狉犪犮狋 犗犫犼犲犮狋犻狏犲 Tousebioinformaticsoftwaretopredictandanalyzethestructureandepitopesoftetraspanin proteinsfrom 犈犮犺犻狀狅犮狅犮犮狌狊犿狌犾狋犻犾狅犮狌犾犪狉犻狊 (EmTspan). 犕犲狋犺狅犱狊 AminoacidsequencesofEmTspanproteins were downloadedfromthencbidatabase.protparam,protscale,sosui,dnastar,signalp,cel PLoc,SOMPA,Net Phos,MotifScan,Phyre2,TMHMM,andotherbioinformaticsoftwarewereusedtopredictandanalyzethephysico chemicalproperties,subcelularlocalization,transmembraneregions,phosphorylationandpost translationalmodification sites,andsecondaryandtertiarystructuresoftheprotein.b andt celepitopeswereanalyzedandpredictedusingthe softwareabcpred,iedb,andsyfpeithi.a molecularevolutionarytreewasconstructedfortspanusing MEGA X. 犚犲狊狌犾狋狊 EmTspanproteinconsistedof236aminoacids,itsmolecularformulawasC1163H1853N291O315S21,anditsinsta bilityindexwas32.77,soitisastableprotein.theproteinhasfourtransmembraneregionslocatedinthecelmem brane.α helicesaccountfor46.19% oftheprotein'ssecondarystructure, β sheetsaccountfor20.34%, β turnsaccount for8.05%,andrandomcoilsaccountfor25.42%.emtspanproteincontainsmanyphosphorylationandpost translation almodificationsites.theproteinhas7b celepitopes,15ctl celepitopes,and8th celepitopes.thetspanof 犈. 犿狌犾狋犻犾狅犮狌犾犪狉犻狊 iscloselyrelatedtothetspanofhymenolepismicrostoma. 犆狅狀犮犾狌狊犻狅狀 Accordingtobioinformatics, EmTspanproteinispredictedtohaveseveralB andt celepitopesandgoodantigenicity.thesefindingscanprovidea theoreticalbasisforimmunodiagnosisofechinococcosisandthedevelopmentofvaccineagainst 犈. 犿狌犾狋犻犾狅犮狌犾犪狉犻狊. 犓犲狔狑狅狉犱狊 犈犮犺犻狀狅犮狅犮犮狌狊犿狌犾狋犻犾狅犮狌犾犪狉犻狊 ;tetraspanin;bioinformatics;epitope % D E " ( 犈犮犺犻狀狅犮狅犮犮狌狊 犿狌犾狋犻犾狅犮狌犾犪狉犻狊 )?- >R?% K [1]?>R Y V W / UE [2], "#!N(? `? [3],b %? ^[4], ;, U b,^ ' 2017 # $ ( " ( " (No.2017YFC ); / 2 ( (No. 2014ZJ719); / (No.2016 wjzd 04) D,E mail:[email protected] () P (1992- ),, %,- \ ( ( ScFG E mail: @qq.com

2 166 8?HI, D E" /, J [5-7], $,?, ?, b [8] ( D E " X F G ( 犈犮犺犻狀狅犮狅犮犮狌狊犿狌犾狋犻犾狅犮狌犾犪狉犻狊 tetraspanin,emtspan)?h BI,N B 6[ T 6[ NM *+, 1 犈犿犜狊狆犪狀 2'-./[;'./ NCBI K DE" Tspan? HIDX HI,FG J S LN D CDS 犈犿犜狊狆犪狀 EXPASY(htps:// ProtParam(htps://web.expasy.org/ protparam/) 7 EmTspanFG?H BI( ^ I T M E _ $ E );C 2 ProtScale (htps://web.expasy.org/protscale/)5 6 7 EmTspanFG?O B ; 2 SOSUI(htp:// harrier.nagahama i bio.ac.jp/sosui/)8 9 EmTspan FG? ; 2 DNASTAR EmTs panfg?o B bb ab? N ( B a b? ;C 2 SignalP4.1 Server(htp:// - _ H EmTspan F G? 3 J K; 2 Cel PLoc2.0,Euk mploc2.0(htp:// sjtu.edu.cn/bioinf/euk multi 2/)_H 7 EmTspan FG?Y6[ M;M SOMPA(htps://npsa pra bi.ibcp.fr/cgi bin/npsa_automat.pl?page=npsa_ sopma.html) NetPhos3.1 Server(htp:// services/netphos/)8 9 EmTspan F G? M $;C 2 MotifScan(htps://myhits.isb sib.ch/cgi bin/motif_scan) 7 EmTspan ;? M $; C 2 ABCpred(htp://crdd.osdd.net/raghava/ab cpred/)diedb,mini(htp://tools.iedb.org/bcel/) NM EmTspanFG? B 6[ NM; 2 SYFPEITHI(htp:// peithi.de/bin/mhcserver.dl/epitopeprediction.htm) 7 HLA A D HLA DRB 0401 M $? T 6[NM; 2 Phyre2(htp:// ic.ac.uk/phyre2/html/page.cgi?id=index)- _ H@ FG? ; 2 TMHMM Serverv. 2.0(htp:// 56 EmTspanFG?X QR89; NCBI 6 E " ( 犈犮犺犻狀狅犮狅犮犮狌狊犵狉犪狀狌犾狅狊狌狊, CDS ) L M ; ( 犆犪犲狀狅狉犺犪犫犱犻狋犻狊犲犾犲 犵犪狀狊,CTQ ) \ P ' ( 犎狔犿犲狀狅犾犲狆犻狊 犿犻犮狉狅狊狋狅犿犪,CUU ) ;1' ( 犜犪犲狀犻犪狊狅犾犻 狌犿,AQQ ) c ` ( 犉犪狊犮犻狅犾犪犺犲狆犪狋犻犮犪, PIS )D % N ` ( 犃狀犮狔犾狅狊狋狅犿犪犱狌狅犱犲 狀犪犾犲,KIH )Tspan X H I, 2 MEGA X 56@ Q,QR) B 7 1 犈犿犜狊狆犪狀 2;'./ M NCBI K, EmTspan F G? S EmuJ_ (LN ), 3967bp, V6,5 V, 236 X H I EmTspan(CDS )?X HIS:mvhftnpdn srvivtrrmalscggkclkflv fnaivfigggivagygvflivktkaags vsvtlpafitfglfligflgcfgacynntcmlktfavivailvaeivcgiv lvyrheemqreikdltahgrnasdplksiyklqeeleccggvgptdws kpypasccksgkenctqpyqqgcavamyeqikdsslafglilivcliqig avicacclakkvneyekv 2 犈犿犜狊狆犪狀 289:3 M ProtParam 56 7 EmTspanFG? ^S C 1163H 1853N 291O 315S 21 ; ITS ,H E _ $ (TheoreticalpI)S 8.44; : 30h(mammalian),>20h(yeast),>10h(Escherichi a);] 32.77,S F G;8 B S ,Z[ S 犈犿犜狊狆犪狀 2 ( ) : 2 ProtScale EmTspan F G? O ( ) B, 1 EmTspan F G N, O SOSUI 7 EmTspanFG? S0.630, S FG 4 犈犿犜狊狆犪狀 2 :[ :ca6 [ 6: 2 DNASTAR EmTspaF G? O B S:3-14,57-61,89-96, , , , ;bB S:5-11,23-25, 42-43,55-61, , , , , , ; a b? :5-14,16-19,22-26,55-62,89-94, , , , ;N( Bab? S:5-10,55-58, , , , , , , ( 2)

3 167 `: O NX?M, O NO( ) B(7 N B, NO B) 1 犈犿犜狊狆犪狀 2 ( ) : Notes:Thehorizontalaxisstandsforthepositionofaminoacidsinthisproteinandverticalaxisstandsforthehydrophicity.Thepositivevalue ofverticalaxisrepresenthydrophobicityandthenegativevaluerepresenthydrophilicity. 犉犻犵.1 犎狔犱狉狅狆犺犻犾犻犮犪狀犱犺狔犱狉狅狆犺狅犫犻犮犿犪狆狅犳犈犿犜狊狆犪狀狆狉狅狋犲犻狀 2 犈犿犜狊狆犪狀 2 :[ :ca6 [ 6:<= 犉犻犵.2 犎狔犱狉狅狆犺犻犾犻犮, 犳犾犲狓犻犫犾犲狉犲犵犻狅狀, 犪狀狋犻犵犲狀犻犮犻狀犱犲狓犪狀犱狊狌狉犳犪犮犲狆狉狅犫犪犫犻犾犻狋狔狅犳犈犿犜狊狆犪狀狆狉狅狋犲犻狀 5 犈犿犜狊狆犪狀 2 B 6CDE SigalP 7 EmTspan F G a 3 J K, S X FG( 3) M Cel PLoc2.0D Euk mploc2.0 7 EmTspanFG M 6[ 25.42%( 4) FG V Tab, / 4 犈犿犜狊狆犪狀 2 F@<= 犉犻犵.4 犛犲犮狅狀犱犪狉狔狊狋狉狌犮狋狌狉犲狅犳犈犿犜狊狆犪狀狆狉狅狋犲犻狀 3 犈犿犜狊狆犪狀 2 B 犉犻犵.3 犛犻犵狀犪犾狆犲狆狋犻犱犲狅犳犈犿犜狊狆犪狀狆狉狅狋犲犻狀犪狀犪犾狔狊犲犱 6 犈犿犜狊狆犪狀 2 F@ M SOMPA 7 c 46.19%, β 20.34%, β 8.05%, 7 犈犿犜狊狆犪狀 2 9[ M NetPhos3.1Server 7,EmTspanFGV 8 X M $ M MX ;7 X M$ M MX ;1 X M $ M 233 M X ( 5) Motif Scan 7,EmTspan F G 13 ; M $, 3 L M$(92-95, , ),2 F G > Ⅱ M $(5-8, ),5 N-. M $(42-47,85-90, , , ) 3 F G > C M$(15-17,56-58, )

4 168 5 犈犿犜狊狆犪狀 2 9 <= 犉犻犵.5 犘狉犲犱犻犮狋犻狅狀狅犳狆犺狅狊狆犺狅狉狔犾犪狋犻狅狀狊犻狋犲狅犳犈犿犜狊狆犪狀狆狉狅狋犲犻狀 8 犅 DE: <= ABCpred5689? B 6 [ N M L a b (S S0.80)?HI :3-16(0.87),27-40(0. 82),43-56(0.86),79-93(0.91),89-98(0.82),94-103(0.81), (0.80), (0.82), (0.89), (0.84), (0.84), (0.83);IEDB EmTspan F G? B NMS:5-10, , , , , , % B FG?O B ab? bb B E1 7, L 7 B 6[ NM(N1) 9 犜 DE <= 2 SYFPEITHI- _H? HLA A ,23MS,EmTspanFG 15 CTL 6[NM; 2 SYFPEITHI- _ H? HLA DRB , 22 M S,EmTspan F G 8 Th 6 [ N M (N 2) N EmTspan HLA A 02-01,^ / HLA DRB 0401 F H Order 1 犈犿犜狊狆犪狀 2 犅 DE <= 犜犪犫犾犲 1 犘狉犲犱犻犮狋犲犱犅犮犲犾犲狆犻狋狅狆犲狊狅犳犈犿犜狊狆犪狀狆狉狅狋犲犻狀 X M Positionofaminoacid NMHI Sequencesofepitope TNPDNS YRHEEMQREIK HGRNAS SIYKLQEE TDWSKPY SGKENCTQPYQ EQIKDS X M HJ Positionof Order aminoacid HLA A ? NMHI EpitoperecognizedbyHLA A 犈犿犜狊狆犪狀 2 犜 DE <= 犜犪犫犾犲 2 犘狉犲犱犻犮狋犲犱犜犮犲犾犲狆犻狋狅狆犲狊狅犳犈犿犜狊狆犪狀狆狉狅狋犲犻狀 Score X M HJ Positionof Order aminoacid HLA DRB ? NMHI EpitoperecognizedbyHLA DRB GLILIVCL YGVFLIVKTTKAAGS SLAFGLIL LKFLVFFFNAIVFIG LLLFLIGFL ILIVCLIQIGAVIC PLLKSIYKL MVHFTNPDNSRVIVT ILIVCLIQI CLKFLVFFFNAIVFI VAILLVAEI VAGYGVFLIVKTTKA SIYKLQEEL LKTFAVIVAILLVAE LILIVCLI VAMYEQIKDSSLAFG ALSCGGKCL IVAGYGVFL TTFGLLLFL AVIVAILLV LVAEIVCGI EIVCGIVLL IVCGIVLLV 23 Score 10 犈犿犜狊狆犪狀 2KF@6>?cA<= 2 Phyre2 - _H@? EmTspanFG? 6 2 TMHMM 5689 EmTs pan 4 X, N C S:32-54, 67-89,99-121, ( 7) 11 RS9@I, M MEGA TspanFG? N JQ 8 DE"? TspanFGD\P '?

5 169 TspanFG b`),- a, c ` TspanFG) B a 6 犈犿犜狊狆犪狀 2KF@<= 犉犻犵.6 犜犲狉狋犻犪狉狔狊狋狉狌犮狋狌狉犲狅犳犈犿犜狊狆犪狀狆狉狅狋犲犻狀 7 犈犿犜狊狆犪狀 2>?cA<= 犉犻犵.7 犘狉犲犱犻犮狋犻狅狀狅犳狋狉犪狀狊犿犲犿犫狉犪狀犲犻狀犈犿犜狊狆犪狀狆狉狅狋犲犻狀 8 ' 犈犿犜狊狆犪狀 ;'./ RS9 犉犻犵.8 犆狅狀狊狋狉狌犮狋犻狅狀狅犳犿狅犾犲犮狌犾犪狉犲狏狅犾狌狋犻狅狀狋狉犲犲犫犪狊犲犱狅狀犈犿犜狊狆犪狀犪犿犻狀狅犪犮犻犱狊犲狇狌犲狀犮犲 V TspanFG 4) X F G K? % L, N. 6[ [9] ChenE [10-13] E(,TspanFG-?N T,^? E Tspan F G F G 2, 6[N( /D3J [14-15], T 6 [ ",! D E _ Tspan FG- DE" 3 _ I.? 2D] C FGI P S? AFGI 5 )?; QE [16] (, ;?FGIT 3J N 6[ E " ( M NetPhos 3.1 Server 7 EmTspanFGV 8 X M$,7 X M $,1 X M $ Motif Scan 7,EmTspan F G V 13 ; M $, 3 L M$;2 FG >Ⅱ M$;5 N. M$;3 FG > C M$ N EmTspanFG- DE"'? 3 _ #? 2 NM AMB?AJ, M NM R16[? /,

6 170 R16[, [17] -? _,T 6[? TCR D B 6[? BCR 8 NM /RS B6[NMD T 6[NM B6[NM% 5~15 X ZL M N( [17] αc D M FG? ",] NM, β T,,- F GN(, NM [18] C2 SOMPA 56 7 β D 8.05% D 25.42% B 6[ NM O B b B a b N ( B a b [19] O B O B X, X X X E?, NM; bb FGI `a?, ; U? X S B?\,% S ab? -? NM; B FGI X V? B?\, NM? ( 6 ( IEDB ABCpred EmTspanFGV 7 B6[ B NM T 6[ N M % 10~20 B 6[ NM])?,T F6[?] / T 6 [ 8 T 6 [ N M S CTL 6[NM( HLAⅠ 8? NM)D Th6[NM( HLAⅡ 8? NM)? HLAⅠ HLA A [20] M SYFPEITHI56? 7 EmTspanFG V 15 HLA A 02-01? N M HLA DRB HLAⅡ,/ % [21-22] SYFPEITHI56? 7 EmTspanFG V8 HLA DRB 0401? NM X αc β as, # [23] ( 89 EmTspanFG V 4 X, S 7 8 M $ B "M@A " > 7 EmTspan F G 3 J EmTspan F G S F G M Cel PLoc 2.0D Euk mploc2.0 7 EmTspanFG M 6 [,@ FG-6[.3J? _ # 2 % " EmTspan? F S"c FGI?AB?( 2M$E@A"H> Q 7 DE"? Tspan F G \ P '? Tspan FGO a 1.,EmTspan DE"? 3 #? 2,^ B 6[ T 6[ N M, B, S DE"??( Z! [1] SchurerJM,GesyKM,ElkinBT,etal. 犈犮犺犻狀狅犮狅犮犮狌狊犿狌犾狋犻犾狅犮狌 犾犪狉犻狊 and 犈犮犺犻狀狅犮狅犮犮狌狊犮犪狀犪犱犲狀狊犻狊 inwolvesfrom westerncanada [J].Parasitology,2014,141(2): [2] TorgersonPR,MacphersonCNL.Thesocioeconomicburdenof parasiticzoonoses:globaltrends[j].vetparasit,2011,182(1): [3] JenkinsDJ.WHO/OIEmanualonEchinococcosis,inhumansand animals:apublichealthproblemofglobalconcern[j].intjpar asit,2001,31(14): [4] W*,XY, C * H,E.& B & B b c % [J]. A Z 9 :,2011,10 (4): [5] KouguchiH,MatsumotoJ,KatohY,etal.Thevaccinationpo tentialofemy162antigenagainst 犈犮犺犻狀狅犮狅犮犮狌狊犿狌犾狋犻犾狅犮狌犾犪狉犻狊 in fection[j].biochemlbiophysrescommun,2007,363(4): [6] V,;. DE' Em18( Q [J].,2009,25(1):67-9. [7] P, V. DE' # Bb EmⅡ/3 Em \ 6 [? ( [J].6 [ 9 :, 2008,24(8): [8] QinC,SunY,DongY.A New MethodforIdentifyingessential proteinsbasedonnetworktopologypropertiesandproteincom plexes[j].plosone,2016,11(8):e [9] JiangX,ZhangJ,HuangY.Tetraspaninsincelmigration[J]. CelAdhesMigr,2015,9(5): [10] ChenL,YuanD,ZhaoR,etal.SuppressionofTSPAN1by RNAinterferenceinhibitsproliferationandinvasionofcoloncanc ercels 犻狀狏犻狋狉狅 [J].TumoriJ,2010,96(5): [11] HoltersS,AnackerJ,JansenL,etal.Tetraspanin1promotes invasivenessofcervicalcancercels[j].intjoncol,2013,43 (2): [12] ChenY,Peng W,LuY,etal.MiR 200aenhancesthemigra tionsofa549andsk MES 1celsbyregulatingtheexpressionof TSPAN1[J].JBiosci,2013,38(3): [13] ChenL,ZhuY,LiH,etal.KnockdownofTSPAN1byRNA silencingandantisensetechniqueinhibitsproliferationandinfiltra tionofhumanskinsquamouscarcinomacels[j].tumorij, 2010,96(2): [14] Yanez MoM,Mitelbrunn M,Sanchez MadridF.Tetraspanins andintercelularinteractions[j].microcirculation,2001,8(3): [15] Levy S,Shoham T.Protein proteininteractionsinthetet raspaninweb[j].physiology,2005,20(4): [16] Q,,,E.FGI ; ( Q [J]. A4 9:,2014,16(9): [17]. [M]. Z,2016: (()177*)

7 ChineseHanpeople[J].GenoProtBioinfo,2003,1(4):304- [22] SaidiS,NabianS,EbrahimzadeE,etal.Identificationand 9. characterizationofcathepsinl likecysteineproteasefrom 犚犺犻狆犻 犮犲狆犺犪犾狌狊 (Boophilus) 犪狀狀狌犾犪狋狌狊 [J].ExpApplAcarol,2016,68 (2): [23] IdreesS,Ashfaq UA,KhaliqS.HCVenvelopeprotein2se quencecomparisonofpakistaniisolateand 犻狀 狊犻犾犻犮狅 predictionof conservedepitopesforvaccinedevelopment [J].JTranslMed, 2013(11):105. [24] AmeghiA,BaradaranB,AghaiypourK,etal.Generationof new M2e HA2fusionchimericpeptidetodevelopmentofarecom binantfusionproteinvaccine[j].advpharmbul,2015,5(sup pl1): [25] TongXL,GuoM,JinM,etal.Insilicoepitopeprediction,ex pressionandfunctionalanalysisofpera10alergenfromthea mericancockroach [J].IntJMolMed,2016,38(6): [26] BaiY,HeSY,ZhaoGH,etal. 犜狅狓狅狆犾犪狊犿犪犵狅狀犱犻 :Bioinfor maticsanalysis,cloningandexpressionofanovelproteintgimp1 [J].ExpParasitol,2012,132(4): [27] WuQ,ZhouP,QianSY,etal.Cloning,expression,identifica [31] FlowerDR.Designingimmunogenicpeptides[J].NatChemBi ol,2013(9): [32] MendezD,Linares M,DiezA,etal.Stressresponseandcy toskeletalproteinsinvolvedinerythrocytemembraneremodeling upon 犘犾犪狊犿狅犱犻狌犿犳犪犾犮犻狆犪狉狌犿 invasionarediferentialycarbony latedin G6PD dificiency [J].Free RadicBiol Med,2011,50 (10): [33] GomezdeLeonCT,Diaz MartinRD,HernandezGM,etal. Proteomiccharacterization ofthesubpelicularcytoskeleton of 犜狅狓狅狆犾犪狊犿犪犵狅狀犱犻 tachyzoites [J].J of Proteomics,2014 (111): [34] PortmanN,GulK.Identificationofparalogouslife cyclestage specificcytoskeletalproteinsintheparasite 犜狉狔狆犪狀狅狊狅犿犪犫狉狌犮犲犻 [J].PLoSOne,2014,9(9):e [35] MelnikowE,XuSL,LiuJ,etal.Apotentialrolefortheinter actionofwolbachiasurfaceproteinswiththe 犅狉狌犵犻犪犿犪犾犪狔犻 gly colyticenzymesandcytoskeletoninmaintenanceofendosymbiosis [J].PLoSNeglTropDis,2013,7(4):e2151. tionandbioinformaticsanalysisofrv3265cgenefrom 犕狔犮狅犫犪犮狋犲 [36] Marquez Navarro A,Perez ReyesA,Zepeda Rodriguez A,et 狉犻狌犿狋狌犫犲狉犮狌犾狅狊犻狊 inescherichiacoli[j].asianpacjtrop Med, 2011,4(4): [28] SeteA,SidneyJ.NinemajorHLAclass Isupertypesaccount forthevastpreponderanceofhla Aand Bpolymorphism [J]. Immunogenet,1999,50(3-4): [29] SouthwoodS,SidneyJ,KondoA,etal.SeveralcommonHLA DR typessharelargelyoverlappingpeptidebindingrepertoires [J].JImmunol,1998,160(7): al.rcb20,anexperimentalbenzimidazolederivative,afectstu bulinexpressionandinducesgrossanatomicalchangesin Taenia crassicepscysticerci[j].parasitolres,2013,112(6): [37] ChenXQ,LiS,HeL,etal.Molecularcharacterizationofsev erinfrom 犆犾狅狀狅狉犮犺犻狊狊犻狀犲狀狊犻狊 excretory/secretoryproductsandits potentialanti apoptoticroleinhepatocarcinomaplccels [J]. PLoSNeglTropDis,2013,7(12):e2606. [30] YanC,WangR,LiJ,etal.HLA A genepolymorphism de finedbyhigh resolutionsequence basedtypingin161 Northern 檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼檼 ( 170*) [18] HoppTP,WoodsKR.Predictionofproteinantigenicdetermi nantsfromaminoacidsequences[j].pnas,1981,78(6): [21] Taneja V,Giphart MJ,Verduijn W,etal.Polymorphism of HLA DRB, DQA1,and DQB1inrheumatoidarthritisin Asian Indians:association with DRB1 0405and DRB1 1001[J]. [19] WangF,YeB.InsilicocloningandB/Tcelepitopeprediction oftriosephosphateisomerasefrom 犈犮犺犻狀狅犮狅犮犮狌狊犵狉犪狀狌犾狅狊狌狊 [J]. ParasitolRes,2016,115(10): [20] TestaJS,VivekanandaS,JulieH,etal.MHCclassI presented HumanImmunol,1996,46(1): [22] WeyandCM,XieC,GoronzyJJ.Homozygosityforthe HLA DRB1aleleselectsforextraarticularmanifestationsinrheuma toidarthritis[j].jclininvest,1992,89(6): Tcelepitopesidentifiedbyimmunoproteomicsanalysisaretar [23], L,,E.6 E' > 34 getsforacrossreactiveinfluenza specifictcelresponse[j]. 7[J]. 9:,2017,12(3): PlosOne,2012,7(11):e