,,,,,
t
Aspiring (cyclooxygenase), (prostaglandin endoperoxide),pgg PGH Aspirin E2 (preoptic hypothalamic region),,,,
, ( ), ( ), ( ),, (27~49) ;,,, 2.0 0.2kg Wistar 200 20g ICR 20 2g, 25 1 55 5 07
00 19 00 SPF Fig. 1~3 Fig. 4 250mg/kg 500mg/kg 1000mg/kg Aspirin 500mg/kg, 1. Pyrogen (C.M.C) 2. Acetylsalicylic.acid.(Aspirin) (Sigma) 3. Sodium.pentobarbital (Sigma) 4. PICB7 (Waters) 5. N-butanol,n-methanol,n-heptane (Sigma) 6. Distilled water
1. 2. Hall open-field(fig.5) 60cm 50cm 80cm 19 80cm 100W 3. High-performance-liquid-chromatography: (HPLC,Waters)Model440 Absorbance Detector Data Module Model 746 Model 717 Autosampler(Fig.6). Detector UV 280nm;Column Phenyl, 30 4. Digital Plethysmometer LE7500 :(Letica-scientific instruments) (Fig.7) 5. Tail-Flick Analgesia Meter :(Columbus Instruments Columbus, Ohio, U.S.A)( Fig.8) 1. : open-field test (47)
20 2g ICR 10, 13 24 Hall open-field 3 (ambulation ) 5ml/kg( ), Aspirin 500mg/kg( ) ( ) 250 mg/kg 500mg/kg 1000mg/kg 15 30 60 90 120 150 180 240 300 360 24 2. 200 20g Wistar 10, 13 24 ( Fig.7)Digital plethysmometer LE7500 5ml/kg( ), Aspirin 500mg/kg( )
( ) 250 mg/kg 500mg/kg 1000mg/kg 30 60 90 120 150 180 240 300 360 24 30 1 (W/L)Carrageenin 0.1 100 100 3. 2.0 0.2kg 10 13 24
5ml/kg( ), Asiprin 500mg/kg ( ),, ( ) 250mg/kg, 500mg/kg, 1000mg/kg. 1 0.2ml/kg pyrogen, 30 60 90 120 150 180 240 300 360 24 4. 200 20g Wistar 10 13 24 Tail-Flick Analgesia Meter ( Fig.8 ) 5ml/kg( ) Aspirin 500mg/kg( ) ( ) 250 mg/kg 500mg/kg 1000mg/kg 30 30 45 60 90 120 180 240
- ( % )= 100% 5. 200 20g Wistar 10 13 24 5ml/kg( ) Aspirin 500mg/kg( ) ( ) 250 mg/kg 500mg/kg 1000mg/kg 30 (51) (cortex) (hypothalamus) ( Fig.9) Waters( ) HPLC Model 440 Absorbance Detector, Data.Model 746 Model 717 Autosampler (monoamine DA, 5-HT, NE ) 100
(Mean S.E.) One Way Anova P 0.05, P 0.01, P 0.001, Fig.10~Fig.12 Aspirin 500mg/kg (ambulation) 24 0~150 180~300 240 Fig.10 1000mg/kg 60 90 120 (P 0.05) 150 (P 0.01) 180 (P 0.001) Fig.11 1000mg/kg 240 (P 0.05) Fig.12
250mg/kg 30,90,120,150,180 (p<0.05),240,300 (p<0.01) 500mg/kg 180 (P 0.05) 1000mg/kg 360 (P 0.001) Fig.13~Fig.15 Aspirin 500mg/kg Fig.13 500mg/kg 180 (P 0.05) 1000mg/kg 240 (P 0.01) Fig.14 1000mg/kg 30 90 180 240 360 24 (P 0.05) 300 0.01) Fig.15 500mg/kg 30 (P 0.01) 1000mg/kg 30 (P 0.001) 300
(P 0.01) Fig.16~Fig.18 39.3 ~39.5 pyrogen 60~180 (40.6 ~41 ) 300 360 Fig.16 1000mg/kg 60 300 (P 0.05) Aspirin 500mg/kg Fig.18 1000mg/kg 60,90 150 180 (P 0.05) Aspirin 500mg/kg Fig.19~Fig.21 Aspirin 500mg/kg
30~45 (P 0.05) 60 ~240 (P 0.01) Fig.19 250mg/kg 45 60 90 120 (P 0.05) 500mg/kg 120 180 (P 0.05) 45 ~24 (P 0.01) Fig.20 250mg/kg 30~120 500mg/kg 30 ~24 (P 0.05) 1000mg/kg 30 180 24 (P 0.05) 45~120 (P 0.01) Aspirin 500mg/kg Fig.21 250mg/kg 30~180 500mg/kg 30 90 120 1000mg/kg 30 120 (P 0.05) 500mg/kg 45 60 1000mg/kg 45 60 (P 0.01) Aspirin 500mg/kg
. 1970 Milton Wendlande (PG) (pyrogen) (10,11,49,50) 1963 Feldberg Meyer norepinephrine(ne) serotonin (5 HT) (17,19,20) NE 5 HT NE 5 HT (16,17.,51,52) (14,15,18,,20) PGE 2 (18) noradrenergic locus coeruleus neuron serotonergic raphe nucleus neuron
(12~20,53) (14,18) NE 4ug 4ug (20) DA (20,54) (5 HT) (15,16) (19,20) PG (25~46) : (1),,, (2), (3), (4),
. E2, PGH2, (26,27,35,45,46,) (26,27), (1), (45,55,56) (2), (26),,, 1000mg/kg 500 1000mg/kg 1000mg/kg,
1. 250mg/kg 500mg/kg 1000mg/kg 2. 1000mg/kg 1000mg/kg Aspirin500mg/kg 3. 1000mg/kg 4. Aspirin500mg/kg Aspirin 5.. 1000mg/kg
1000mg/kg. ( ). Aspirin 6.