2015 1 20 2010 2014 DNA CRISPR
7000 4000 ETC 93
BIG BIG 1 104 100101 010-84097548 www.big.ac.cn zhouml@big.ac.cn Tomas Lindahl
DNA K-mer PacBio DNA Tomas Lindahl Tomas Lindahl
10 26 2015
聚焦 9 8 100 30
9 2 10 30
聚焦 10 22 2015 3 16 20
11 5
聚焦 10 22 2015 3 48 30
10 23 - BIG - BIG - BIG - BIG
聚焦 12 23 -
9 11 2015 9 25 2015
聚焦 2016 10 26
10 28 BIG Olson Maynard Olson Olson 2 Olson Olson 1988 BIG YAC Olson Olson 2011 Olson
聚焦 5 science nature BIG 11 25 BIG 2 1997
12 24 11 80 2007 40
聚焦 10 12 8
2015 11 16 2015 50 11 16 2015 95 2011 4 340
聚焦 CasTech CasTech CasPoint CasPoint, 10 30
12 11 2015, 2015 2014
聚焦 Lindahl 2015 10 7 Genomics Proteomics & Bioinformatics GPB Tomas Lindahl Aziz Sancar Paul Modrich 2015 Lindahl 2009 GPB DNA 2015 Lindahl GPB Lindahl DNA Lindahl 2013 Lindahl GPB DNA My Journey to DNA Repair
12 18 2015 2015 2015 58 Web of Science SCI Genomics,Proteomics&Bioinformatics GPB 2015 SSCI 2013 2014 2015 GPB 3500 59 Web of Science GPB CI 5% GPB 175 54 CI
学术 300 2000 3.5 10 10000 100 2015 11 11
( ) 20 80 30 973
学术 PSA DNA PSA PSA 4-10ng/ml 25%-40% PSA Identification of specific DNA methylation sites on the Y-chromosome as biomarker in prostate cancer Oncotarget marker 66 Y 973 Y 6 DNA ROC 94.6% 78.3% PSA DNA ROC
Li and Durbin(2011) PSMC 2 Kingman 300 Kingman - robustness 2015 11 Molecular Biology and Evolution 1 TNSFS A Gazave, B TNSFS
学术 Differential Responses to Lithium in Hyperexcitable Neurons from Bipolar Patients induced pluripotent stem cell, ipsc BD hippocampal dentate gyrus granule cell-like neurons LR NR Bipolar disease, BD Hyperexcitability WHO BD BD ipsc BD BD BD - BD BD
UCL K-mer K-mer KGCAK K-mer Biology Direct motif Ensembl Phytozome NCBI K-mer K-mer 8000 DNA cdna CDS ncrna K 2 10 K 1 5 K-mer K-mer K-mer K-mer K-mer
学术 Web API IC4R(Information Commons for Rice) 2015 10 Nucleic Acids Research IC4R 5000 RNA-Seq Wiki Community Curation IC4R RAP-DB MSU-RGAP 863
ICGC SNV TCGA 2013 CasBreak CasTech EMBL ICGC-TCGA Dream Somatic Mutation 98.3% F1 Challenge SMC SMC SV F1 98.5% WGS ICGC 14 49 MD Anderson SMC CasTech HKU-BGI BAL CasPoint CasTech SNV SV 99.8% F1 Bina Roche Rutgers - BROAD
学术 PacBio PacBio SMRT cdna Cell 1Gb reads PacBio read 14kb N50 19kb cdna PacBio LncRNA 3. 1. de novo PacBio read de novo 5Mb DNA SMRT Cell finish 5 IPD ratio,, Contig N50 100kb de IPD ratio novo 50 PacBio m6a, PacBio 5mC 5hmC 4mC 30 PacBio scaffold 10 PacBio 2..PacBio read subread cdna PacBio DNA PacBio BAC GC DNA
Tomas Lindahl 2015 Tomas Lindahl DNA 10000 DNA DNA DNA DNA DNA DNA / DNA i - DNA -DNA 3- DNA DNA ii - nobelprize.org DNA AP 1975 1976 Walter Verly iii EB O6- - DNA MGMT Ada EB DNA DNA DNA iv DNA AlkB 20 70 80 Barbara Sedgwick DNA ErlingSeeberg DNA FTO ALKBH5 3- RNA m6a
学术 DNA DNase III DNase IV TREX1 FEN1 FEN1 5-3 DNA TREX1 DNA 3-5 TREX1 SLE Aicardi-Goutières syndrome Yanick Crow 2007 DNA DNA TREX1 Tomas Lindahl. Genomics, Proteomics & Bioinformatics, Volume 11, Issue 1, February Deborah Barnes 2013, Pages 2 7 DNA DNA Ada DNA DNA Ada 1986 ATP XPA ICRF DNA Clare Hall ATP NAD Cancer Research UK DNA DNA DNA I DNA III DNA IV Lee Johnston DNA I 1990 - Genomics, Proteomics & Bioinformatics 2013 DNA I
DNA 90 49 28 DNA DNA
科普 CRISPR/Cas9 MSTN DNA CRISPR/Cas CRISPR/Cas9 CRISPR Clustered Regularly Interspersed Short Palindromic Repeats 1. CRISPR/Cas9 DNA 2015 A CRISPR/Cas9 2015 1CRISPR CRISPR/Cas9 PAM Protospacer adjacent motifs- CRISPR/Cas9 ZFN DNA TALEN DNA PAM PAM CRISPR
2 CRISPR CRISPR Cas CRISPR CRISPR RNA Cas PAM DNA DNA DNA CRISPR/Cas9 RNA Cas9 2CRISPR/Cas9 CRISPR/Cas9 DNA RNA PAM DNA 3 RNA guide RNA, grna Cas9 RNA( RNA RNA1 RNA2) Cas9 PAM RNA NGG N PAM
科普 ips CRISPR/Cas9 ips CRISPR HIV Nature CRISPR/Cas9 3CRISPR/Cas9 Cas9 DNA CRISPR/Cas9 DNA DNA DNA CRISPR/Cas9 CRISPR CRISPR/Cas9 CRISPR/Cas9 CRISPR/Cas9 RNA DNA [1]Doudna, J.A.; Charpentier, E., CRISPR/ Genome editing. The new frontier of genome Cas9 engineering with CRISPR-Cas9. Science, Cas9 2014, 346, (6213), 1258096.[ ] CRISPR/Cas9 [2] Fang, R.; Chang, F.; Sun, Z.-L.; Li, N.; Meng, Q.-Y., New Method of Genome Editing Derived From CRISPR/Cas9. CRISPR/Cas9 ActaAgronomicaSinica, 2013, 40, (8), 691. ips
RNA U DNA RNA RNA RNA DNA U U you DNA RNA mrna DNA 5- m5c 5- hm5c mrna RNA DNA mrna 5-7- GTP DNA mrna5 - RNA T DNA U RNA A G C DNA RNA DNA A T,G C A U G C
文化 10 7 2005 9 2015 Tomas DNA Lindahl Paul Clare Hall Modrich Aziz Laboratories Sancar DNA DNA 2000 WHO DNA DNA DNA DNA DNA DNA DNA DNA DNA
2005 11 68 DNA. (Tim Hunt)(2001 ) 2007 11 30 110 20 25 10 DNA 2008 11
文化 Genomics Proteomics & Bioinformatics (GPB) 2010 2013 My Journey to DNA Repair GPB (http://www.sciencedirect.com/science/article/ pii/s1672022912000976) 2014 DNA Beverly 6 (Arne Klungland) Tomas Lindahl Conference on DNA repair DNA DNA DNA RNA RNA m6a 6- RNA RNA 2009 10 21 2008 11 20, (I still enjoy very much doing science. It is pleasure, it is very interesting and it is stimulating. It changes all the time. I would like to be here around hundred years to see how science develops.-my Journey to DNA Repair) 2009
研究员专访 编者按杨运桂博士是中国科学院 百人计划 获得者, 现任中科院北京基因组研究所研究员 1995 年毕业于复旦大学生命科学学院,2000 年获得中科院上海药物所理学博士学位 ; 后又分别在世界卫生组织国际癌症研究署 ( 法国里昂 ) 和英国癌症研究署从事博士后研究, 目前主要研究领域是 RNA 甲基化表观转录组特征 调控和功能研究 在学生眼里, 杨运桂研究员平易近人, 直率认真而又不失风趣幽默, 对学生既严格又关心 他办公室的墙上挂满了已毕业学生的照片, 而办公桌上的一角摆放着家人的照片 在与他长谈中, 笔者不仅能感受到他对科学探索的执着与坚定, 也能感受到他对对生活的积极与乐观 PI 100
文化. (Tomas Lindahl) DNA RNA DNA 1974 PNAS 1992 Nature DNA RNA RNA DNA RNA RNA RNA The Age of Wonder 6 9
Paper
文化 11 25
11 27
文化 1 1 2 2 1 2 11 2 11 2015 5 4 5 73 1 1
ready to submit under review in press 3. Manuscript received by editorial office 1. Ready to submit 4. With editor Awaiting editor assignment Editor assignment Technical check in progress 2. Submitted to journal Editor declined invitation
文化 50% Decision letter being prepared 9. Revision submitted to journal Review(s) invited 10. Accepted 5. Under review 11. Transfer copyright form decline 12. Uncorrected proof 6. Required reviews completed 13. In press, corrected proof 14. Manuscript sent to production 7. Evaluating recommendation 15. in production decision 8. Minor revision/ Major revision Minor revision Major revision
文化 :!?!
2015 12 25 Tara-cry cry