Vol.5 APRIL 2010 簡訊 主題文章 2010 年 第四屆亞洲實驗動物聯盟大會 籌備進度說明 11 (The Asian Federation of Laboratory Animal Science Associations; AFLAS) 00 10 (00) 1010 11 目錄 2 主題專欄 010 5 最新動態 00 8 年度活動 10 國際交流 0 Charles River Laboratory RIKEN BRC Prof. Shigeharu WAKANA Prof. Robert Hoffman 22 技術專欄 29 活動預告 The Jackson Laboratory 30 用戶資訊 31 繽紛生活
主題專欄 2010 年台灣實驗動物科學界最大盛事 第四屆亞洲實驗動物聯盟大會 簡介 王歆婷 張嘉修 張維正 AFLAS 11 010 (The Asian Federation of Laboratory th AFLAS 00 Animal Science Associations; AFLAS) (International Council for (The Asian Mouse Mutagenesis Resource Association; AMMRA) 第四屆亞洲實驗動物聯盟大會 AFLAS 001 00 (AFLAS) (CSLAS) AFLAS AMMRA 00 1 th AFLAS Laboratory Animal Science; ICLAS) ICLAS th AFLAS (workshop) AAALAC International Charles River Laboratories Mount Sinai Hospital, Canada 1 CRO GLP 010 AFLAS Vol.5 APRIL 2010
主題專欄 010 11 1:10 1:10 1 10 10 1 1 1 1 1 1 1:10 1:10 010 11 Break time Keynote Section Break time Keynote Section Lunch Keynote Section Coffee Break Break time 010 11 10 Keynote Section CRO GLP Break time Lunch Coffee Break Break time CSLAS AFLAS Close Door Meeting AMMRA Close Door Meeting AMMRA Close Door Meeting 010..1 010..1 NTD$,000 NTD$,00 NTD$1,00 NTD$,000 m 010..1 010..1 NT$0,000 NT$0,000 NT$,000 NT$,000 www.aflas010.org 010 AFLAS 大會重要日期 : 註冊報名 :2010.311 論文摘要徵稿 :2010.35 參展報名 :2010.59 大會日期 :2010.11.911 2009 年十大服務項目全數完成 戴文玉 項 目 成 效 1.ELISAH1 ECUN ReoEDIM ECUN 1.IFA Rat Mice 1 1. hemolytic streptococcus.klebsiella oxytoca http://www.nlac.org.tw/n/n1.asp 1...CBL/ BALB/c 00 10 10 00 010 11 11 Keynote Section Mutant Mouse Phenotyping 1 Break time Lunch Coffee Break 第四屆亞洲實驗動物科學聯盟大會聯絡方式 (0) (0) AFLAS010@nlac.org.tw th AFLAS 官方網站 http://www.aflas010.org AFLAS 總會官方網站 http://www.aflasoffice.org 00 00 10 10 00 1 1 Vol.5 APRIL 2010
最新動態 新年度 新視野 組織架構新佈局 動物中心從 心 出發 陳月筵組長 00 00 10 0 010 1 1 00 1 1 010 1 1 1 Vol.5 APRIL 2010
年度活動 對外服務說明會熱烈迴響 圓滿達成雙向交流目標 1 實驗動物科普教育成果分享 1 1 林慧珍 饒心儀 林慧珍 1 10 10 10 1 R 11 1 1 10 1 Vol.5 APRIL 2010
國際交流 參加第 60 屆美國實驗動物年會 (American association for laboratory animal science, AALAS) 1 梁鍾鼎副研究員 (1) () () () AAALAC : 1.Technical Trade Presentation: 1 Mouse Phlebotomy: A Comparison of Facial Vein and RetroOrbital Sinus Collection Methods A.Sample integrity: CK ALT AST ALP LDH TBIL P Fe Mg B.Mouse comfort: corticosteroid 10 Vol.5 APRIL 2010 11
國際交流 C.Cost: What's Your Diagnosis? 10.Seminar: 1 Rodent Genetic and Health Mornitoring Programs in Latin America FELASA ELISA IFA MFIA HA IHA Parvovirus Norovirus Helicobacter Parvovirus RTV 0% in Brazilian rat colony.special Topic 1 Operant Conditioning of Exotic Animals for Veterinary Procedures G o o d L a b o r a t o r y P r a c t i c e ( G L P ) Regulations (GLP) GLP.Panel Discussion 1 Pathology Quiz Bowl 0 Institutional Approaches to the Control and Eradication of MPV MPV MPV MPV MPV 控制 :A. B. 清除 :A. B. Depopulate, decontaminate, repopulte Test and cull 考驗 : 病例討論 : MPV : grain grain Parvovirus A. B. 0 C. MPV grain MPV Denver Zoo IVC (Targeted Sentinels) 12 Vol.5 APRIL 2010 13
國際交流 第 23 屆國際哺乳動物基因體年會 靖永皓副研究員 IMGC 010 IMGC Cold Spring Harbor Mouse Genetics & Genomics: Development & Disease (International. Modeling Disease I Metabolism and Mammalian Genome Society) Physiology. Modeling Disease II Development. Modeling Disease III Development and Bioinforamtics Genomics Research Neoplasia. Scientific Approaches to Animal Husbandry Bioinforamtics Tools Resources. Population Genetics, Comparative Bench Scientist Genomics and Evolution resources (genetic manipulation) 1 La Jolla Torrey Pines La Jolla Torrey Pines PGA Tour Torrey Pines Epigenomes massively parallel sequencing Dr Karen Svenson The Jackson Laboratory, Director of The Heart, Lung, Blood and Sleep disorder center T h e S c r i p p s R e s e a r c h I n s t i t u t e Professor Bruce Beutler Cornell University Professor John Schimenti Kerry Schimenti 1. Large Scale Resources. Infectious Disease, Host Resistance and 00~0 Epigenetics. Neuroscience, Behavior, and Sensory Riken Perception Jackson Laboratory 1 McLaughlin Research Institute Professor Teresa Gunn Dr. Shigeharu Wakana IMGC University of Pennsylvania Professor Maja Bucan 14 Vol.5 APRIL 2010 15
國際交流 借鏡 Charles River Laboratory 提昇本中心診斷實驗室病原檢測能量 謝偉鈞 實驗動物科學應用計畫審議工作坊 優質科學與良好動物福利課程 王歆婷 張維正 Charles River Laboratory (CRL) CRL 1 CROs Clinical Research Organization CRL 1 00 RSPCA 10.00 1.00 1. RSPCA CRL TAF Dr. Maggy Jennings RSPCA Mr. Paul Littlefair Dr. Derek Fry Charles River Laboratory lecture C R L H M Department and Parasitology IACUC CRL 1 Bacteriology Molecular Diagnosis RSPCA 16 Vol.5 APRIL 2010 17
國際交流 駐台使節代表科學園區之旅 駐台使節團參訪國家實驗動物中心南部設施 丘一芳 11 10 IVC 1 18 Vol.5 APRIL 2010 19
國際交流 中國山東省實驗動物學會參訪 丘一芳 日本 RIKEN BRC 小鼠診所 Prof. Shigeharu WAKANA 參訪國家實驗動物中心 林慧珍 00 1 1 00 10 Prof. Shigeharu WAKANA RIKEN BRC 1 Prof. Shigeharu WAKANA RIKEN BRC 美國加州大學聖地牙哥分校 Prof. Robert Hoffman 受邀參訪動物中心 林慧珍 1 Prof. Robert Hoffman 11 1 Prof. Robert Hoffman 20 Vol.5 APRIL 2010 21
技術專欄 評估衛兵鼠於獨立換氣飼養系統中以接觸髒墊料監測病原之效力 施艾 以 RNA 干擾法產製條件式基因抑制鼠技術平台 蔣思澈 (Murine Norovirus, MNV) Helicobacter spp. (Entamoeba spp.) (Trichomonads)(Helicobacter spp.) (Sentinel Mice) IVC 11~1 MNV Entamoeba spp. Trichomonads (Individually Ventilated Cage, Swiss(outbred) IVC) IVCHelicobacter spp. 1~ 0PCR RNA interferencernai BALB/cAnN. Pkd1 CgFoxn1nu/nu(BALB/cnu/nu) BALB/cAnN. CgFoxn1nu/+(BALB/cnu/+) Swiss Webster (Swiss)MNV Entamoeba spp. Trichomonads Helicobacter spp. 1 microrna microrna (emerald GFP) coding sequence polya signal ubiquitin C promoter (DsRed) 11 1MNV ELISAEntamoeba spp. Trichomonads MIF Helicobacter spp. PCRMNV loxp Cre recombinase Cre recombinase Swiss BALB/cnu/+ Entamoeba spp. Trichomonads MIF microrna Pkd1 Cre 22 Vol.5 APRIL 2010 23
技術專欄 建立偵測小鼠肝炎病毒之反轉錄聚合酶連鎖反應技術 施艾 建立 NOD.CB17 Prkdc scid /J 小鼠胚幹細胞 秦咸靜 (Mouse Hepatitis Virus, MHV) RNA (Reverse transcription polymerase chain reaction, RTPCR) Yamada et al,. Exp.Anim.() P1+N bp 0bp bp RNA TMEV MNV RTPCR (Enzymelinked immunosobent assay, ELISA) MHV ELISA RTPCR.% RTPCR 0%0% MHV (B/c B/c nude) MHV RTPCR (B/c nude) (B/c) MHV MHV RTPCR P10+N Yamada et al,. Exp.Anim.() bp 1+ Nested PCR 1copy MHV F+MHVR F. R. Homberger et al,. J Clin Microbiol. (1) bp (Rat Sialodacryoadenitis Virus, SDAV) (Embryonic Stem cells, ES cells) (Inner Cell Mass, ICM) (Totipotency) 10 NOD SCID (Alkaline Phosphatase, AP) CD SSEA1 Oct/ Nanog R1 1 NOD SCID (NS)CD/SSEA1 NOD SCID 24 Vol.5 APRIL 2010 25
技術專欄 建立誘發性轉錄發光報導小鼠 NFKB NFKB NFKB NFKB NFKB (NFKBREluc) (in vivo image system, IVIS) NFKB FVB/NJNarl 以基因改造小鼠探討脂肪發炎反應和胰島素抗性之關聯 1. 00 0 0% 蔡曜聲 Tolllike Receptors (TLRs) TLR TLR 蔡佩珍 NFKB LPS NFKB IVIS LPS NFKB NF KB 實驗動物房舍 廠務管理及節能措施之 實務經驗與技術應用 鍾勝邦 * 陳景富 陳月筵 劉仁勗 余俊強 翁聰標 梁善居 0~ ~ Rs R RO 26 Vol.5 APRIL 2010 27
技術專欄 精彩活動預告 美國傑克森實驗室 The Jackson Laboratory 小鼠飼育管理課程即將於八月在台開課 饒心儀 ( T h e J a c k s o n What's in a Name Laboratory) () Colony Setup and Breeding Strategies () Genetic Background Effects on 1~1 0% ~ Phenotype () Reproductive Biology of the Laboratory Mouse () Using Assisted Reproductive Technologies (ARTs) to Rederive, Rescue, and Rapidly % ~1 1,1,00,1 (Mouse Colony Management: Principles and Practices) Expand Colonies () Cryopreservation: A Cool Approach to Colony Management () Practical Guidelines for Maintaining Mouse Health (10) Recovering from Disease Outbreaks (11) Guidelines for Production Colony Construction and Maintenance 0% %1 (1) New Mouse Models and How to Find Them: Electronic Resources for Mouse Geneticists 010 http://www.nlac.org.tw/workshop/ 1 (1) Back to the Future From Fancy Mice to Mendel: A Mouse Genetics Primer () Genetic Quality Control () Mouse Genetics and Strain Nomenclature: HsinYi Yao Tel Jenny Liu Tel Email workshop@nlac.org.tw 28 Vol.5 APRIL 2010 29
用戶資訊 繽紛生活 服務品質問卷客戶常見問題回覆 2009 年動物中心耶誕 party Q1 A Q A Q A Q A Q Trichomonads A 100Trichomonads PCR Q A ~% Q A 戴文玉 Q A Q A 1 Q10 A Q11 CBL/ A CBL/ CBL/ http:// www.nlac.org.tw/order/order.aspx Q1 A 1~ 林佳祺 00 party party party RAP 30 Vol.5 APRIL 2010 31
分類服務項目聯絡人 實驗動物供應一般品系陳惠芸 01 00 0 0 北部對外技術服務 0 0 0 南部設施對外技術服務 IVIS Animal Hotel 0 010 01 010 www.nlac.org.tw/ 11529 台北市南港區研究院路二段 128 號 ( 中央研究院院區 ) 臺北市南港郵政 186 號信箱 Tel:0226518900 Fax:0227895588 74147 台南縣新市鄉南科二路 17 號 ( 南科育成中心斜對面 ) Tel:065108200 Fax:065055748