JournalofNingxiaMedicalUniversity : - ( ) - - OA 杨勇, 吴世栋, 张小钰, 金群华 (., ;., ) : 目的 (OA), 方法 SD ( ),,,, Mankin MMP- 结果 Mankin MMP-, (P<. ), (P<. ), (P<. ) 结论 OA, : ; ; ; :R :A (osteoarthritis, OA) OA, OA OA,, OA. (matrix metaloproteinase, MMP- ) Santa cruz, EnVision (PeriodicAcidSchif) (Alcian Blue, AB-PAS) SD. SD,, ~ g, :,. % mg/ kg, :. cm,,,,,, :,,,,,,, h : - - : ( -), ( ),, E-mail:yangyong @.com :, ( ),,, E-mail:jinqunhua@sina.com., cm,, %, h,, PBS, h, % EDTA, h,,,,, μm HE AB-PAS MMP-.. HE AB-PAS,, ; Mankin [ ],. HE Olympus ( ) Olympus, imageproplus (Version., MediaCybernetics, SilverSpring, MD) (BV/TV),, / ( + ) %,. MMP-,, (ph. ), MMP- ( Santacruz ),, PBS, EnVision, min, PBS, DAB,,,
,. OA, PBS :,,, =( / ) % ~ % (-), % ~ % (±), % (+), % (+ +), % (+++). ±, SPSS.,,, P<.. :,,,,, :,,,,,,,,,,,,,,,,, (, )., Mankin :, :,, ; ;,, ; ;AB-PAS :,,,,,,,, AB-PAS, (, )., (P<. ); (P<.. ); (P<. ); (P<. ),, (BV/TV, %). ±.. ±.. ±.. ±. *. ±. **. ±. ** *P<., * *P<. ; P<. ; P<. (P<.. ). MMP- ( ) :, Mankin :,. ±.. ±.. ±. (P>. );. ±. *. ±. **. ±. * *, *P<., **P<. HE,,,, (P<. );, (P<. ),, ;AB-PAS, MMP- - ± + ++ +++ (ACLT) ( ~ OA [ ],,, OA g), OA [ ],,, ( )
; ( ),,,, ;,,,,,, DaubsBM [ ] OA OA, (MMPs), MMPs-TIMP( ),, [ ] MMP-, Ⅱ % ~ % Ⅱ, MMP- OA [ ] MMP-,, MMP - OA, OA OA [ ] OA OA BoterSM [ ] micro-ct,,, HayamiT [ ] OA,,,,, OA,,,, OA,,,,, OA, OA, OA [ ], OA,,,, : [ ] MankinHJ, DorfmanH, LippieloL, etal.biochemical andmetabolicabnormalitiesinarticularcartilagefrom osteo-arthritichumanhips. I.Corelationofmorphologywithbiochemicalandmetabolicdata[ J].JBone JointSurgAm,, ( ): -. [ ] RoachH, TileyS.ThePathogenesisofOsteoarthritis [ A].In:BronnerF, MaryC(Eds).BoneandOsteoarthritis[ M].London:British LibraryCataloguingin PublicationData, : -. [ ] AmeyeLG, YoungMF.Animalmodelsofosteoarthritis:lesonslearnedwhileseekingthe HolyGrail [ J]. CurOpinRheumatol,, ( ): -. [ ] DaubsBM, MarkelMD, ManleyPA.Histomorphometricanalysisofarticularcartilage, zoneofcalcifiedcartilage, andsubchondralboneplateinfemoralheadsfrom clinicalynormaldogsanddogswithmoderateorsevere osteoarthritis[ J].AmJVetRes,, ( ): -. [ ] KevorkianL, YoungDA, DarahC, etal.expresion profilingofmetaloproteinasesand theirinhibitorsin cartilage[ J].ArthritisRheum,, ( ): -. [ ] JohnsonAR, PavlovskyAG, OrtwineDF, etal.discoveryandcharacterizationofanovelinhibitorofmatrix metaloprotease- thatreducescartilagedamageinvivowithoutjointfibroplasia sideefects[ J]. JBiol Chem,, ( ): -. [ ] KarsdalMA, LeemingDJ, etal.shouldsubchondral boneturnoverbetargetedwhentreatingosteoarthritis [ J].OsteoarthritisCartilage,, ( ): - [ ] BoterSM, vanog, WaarsingJH, etal.quantification ofsubchondralbonechangesinamurineosteoarthritis modelusingmicro-ct[ J].Biorheology,, ( - ): -. [ ] HayamiT, PickarskiM, ZhuoY, etal.characterizationofarticularcartilageandsubchondralbonechanges intheratanteriorcruciateligamenttransectionandmeniscectomizedmodelsofosteoarthritis[ J].Bone,, ( ): -. [ ] HayamiT, PickarskiM, WesolowskiGA, etal.the roleofsubchondralboneremodelinginosteoarthritis: reductionofcartilagedegenerationandpreventionof osteophyteformationbyalendronateintheratanterior cruciate ligamenttransection model[ J]. Arthritis Rheum,, ( ): -. ( : )
,. OA CharacteristicsofArticularCartilageandSubchondralBoneChanges inratanteriorcruciateligamenttransaction YANGYong, WUShi-dong, ZHANGXiao-yu, JINQun-hua (Dept.ofOrtherpeadics, theafiliatedhospitalofningxiamed.univ., Yinchuan ) Abstract:Objective Toestablishanosteoarthritismodelinratkneesbytransactinganteriorcruciateligament, andcharacterizingsubchondralboneremodeling, cartilagedamageduringthediseaseprogresioninthe modelsofsurgicalyinducedoa.methods ratsweredividedintothreegroup( weeks weksand wekspost-surgerygroup).rats'rightkneejointsweresubjectedtoanteriorcruciateligamenttransection (ACLT), whiletheleftkneeservedascontrol.ratsweresacrificedandthetibilplateswereharvestedwhich werestudiedinbothgros-morphologicalandpathohistological, atthesametimecoimmunostainingformatrix metaloproteinase (MMP- )wasperformedtoinvestigatetheprogresionofcartilagedegradetion.results Thedegenerationofarticularcartilageofthesurgeryknesgotworsebytime.surgeryknesMankin's scoresandmmp- expresionsweresignificantlyhigherthanthoseinthesham knees(p<. ).Histomorphometricanalysisrevealedsubchondralboneloswithin wekspostsurgery(p<. )folowedbysignificantincreasesinsubchondralbonevolumerelativetosham upto weekspost-surgery(p<. ). Conclusion WACLTonratscaninduceOsteoarthriticchangeswhichinvolvecartilageandsubcondralbone. Thismodelhavethemeritsofeconomical, convenientandshort-terming. Keywords:osteoarthritisOA;animalmodel;subcondralbone;matrixmetaloproteinase ( 上接第 页 ) EfectofChronicUnpredictableMildStresonNeurokinin- ReceptorinCaudatePutamenofRats MAJiang-bo, HEZhong-yi, LIJun-ping, etal (NingxiaMed.Univ., Yinchuan ) Abstract:Objective ToexploretheasociationofthemorphologicchangeofNK receptorincpuofratsand themechanismofdepresionusingcumsmodel.methods Sixmaleratswereexposedtochronicunpredictablemildstres(CUMS)toinducedepresionfor weks.exposuretocumssignificantlyreducedopenfieldexploration, rearing, sucroseconsumptionin hours, immovabilitytimeofforceswimmingandles weightgainwithage.results Thebehaviorrepresenteddepresionafterstresof daysinthemodegroup andtheindexesincludedinopen-fieldexploration(. ±. ), rearing(. ±. ), sucroseconsumptionin hours(. ±. )ml, immovabilitytimeofforceswimming(. ±. )sand weightincreasing(. ±. )g, andalabovethevaluesofindexesweresignificantlylowerinthemode groupthantheminthecontrolgroup(p<. orp<. ).Additionaly, thenumbersofnkipositivereceptorincpuinmodegroup(. ±. )weresignificantlymorethantheseinthecontrolgroup(. ±. )(P<. ).Someneuronbodybecamebigerandtheiraxonordendronbecamewider.Conclusion NK receptorincpuwasprobablyrelatedwiththemechanismofdepresion. Keywords:caudateputamen;chronicunpredictablemildstres;neurokinin- receptor;immunohistochemistry