热 带 海 洋 学 报 JOURNAL OF TROPICAL OCEANOGRAPHY 2011 年 第 30 卷 第 1 期 :107 112 海 洋 生 物 学 http://jto.scsio.ac.cn; http://www.jto.ac.cn 鱾 肖 云 朴 1, 徐 善 良 2, 孙 敏 3, 吕 慧 明 3 (1., 325400; 2., 315211; 3., 315211) : Girella leonina : 1) 6 2), 10.00μm 6.40μm, 8.80μm 5.80μm,, 3.20μm 2.60μm, 2.80μm 2.50μm;,,,, 3),,,, 4,, 4),,,, 9+2 : Girella leonina; ; ; 中 图 分 类 号 : Q959.212 文 献 标 识 码 : A 文 章 编 号 : 1009-5470(2011)01-0107-06 Ultrastructural changes in Girella leonina during spermatogenesis XIAO Yun-pu 1, XU Shan-liang 2, SUN Min 3, LÜ Hui-ming 3 (1. Ocean and Fisheries Bureau of Pingyang County, Pingyang 325400, China; 2. Key Laboratory of Marine Biotechnology of Zhejiang, Ningbo 315211, China; 3.College of Life Sciences, Ningbo University, Ministry of Education, Ningbo 315211, China) Abstract: The ultrastructural changes of the germ cells and sperm during spermatogenesis of Girella leonina were observed by transmission electronic microscope. The results are as follows. (1) The spermatogenesis of Girella leonina included six stages: primary spermatogonium, secondary spermatogonium, primary spermatocyte, secondary spermatocyte, spermatid, and sperm. These stages can be divided into three periods (before, during, and after) when the spermatid differentiated into sperm. (2) A primary spermatogonium was the largest of various stages of germ cells, and its cell was about 10.00μm 6.40μm and the nuclear counted 8.80μm 5.80μm. The spermatid was the smallest, and its cell was about 3.20μm 2.60μm and the nuclear counted 2.80μm 2.50μm. With spermatogenic cells continued to divide and mature, their size gradually reduced, the nuclear chromatin tended to concentrate, electron density increased, extent of staining deepened, and nuclear position, nucleolus, mitochondria also correspondingly changed. (3) Mitochondria were the main organelles in the germ cell, which gradually reduced the number in the process of spermatogenesis. At last, there were only four mitochondria in the mature sperm. On the contrary, the volume of mitochondria had kept increasing, and its cristae became clearer and longer. (4) As most kinds of teleostean, each sperm of Girella leonine consisted of a head without an acrosome, a midpiece, and a long tail that contains the lateral fins and a conventional 9 + 2 axoneme pattern. Key words: Girella leonina; spermatogenesis; ultrastructural; mitochondria 收 稿 日 期 2009-01-16; 修 订 日 期 2010-05-14 基 金 项 目 (2007C22044) 作 者 简 介 (1975 ),,,, E-mail: xiaoyunpu@21cn.com 通 信 作 者 : Tel: 13705749890, E-mail: xushanliang@nbu.edu.cn
108 Vol. 30, No. 1 / Jan., 2011 Girella leonina,, 鱾,, [1],,,, [2] 鱾 [3] [4] [5], [6 7],,, 1 1.1 2008 3 2008 4 16, 21.77 25.09cm, 414.33 482.83g 1.2,, 1mm 3, 0.1mol L -1 ph 7.4 2.5% (4 ), 1 1 2h,, Epon 812, POWERTOME XL,, H-7650 1.3 =( )/( ) [8] 2 2.1 1,,, ;,, 6 2.2 2.2.1 初 级 精 原 细 胞, 10.00μm 6.40μm,, 8.80μm 5.80μm( 1a), 0.798,,,,, ( 1a), ( 1b) ( 1c) 2.2.2 次 级 精 原 细 胞, 9.00μm 6.20μm, 6.40μm 5.60μm, 0.642,, ( 1d 1e),,,,,,,, 2.2.3 初 级 精 母 细 胞 ( 1f),, 7.50μm 3.20μm,, 3.80μm 2.60μm, 0.412,,, ( 1g),,, ( 1h),, ( 1h 1i),,,,,,,,
: 109 1 a., ; b., ; c. ; d., ; e. ; f. ; g., ; h., ; i. ; j.,, ; k. ; l., ; m., ; n. ; o., G1: ; G2: ; ER: ; M: ; N: ; NU: ; SC: ; SE: ; SG1: ; SG2: Fig. 1 Ultrastructural observation in Girella leonina during early spermatogenic cell
110 Vol. 30, No. 1 / Jan., 2011, 3 4 ( 1i),,,,, ( 1j),,,, ( 1k),,,,,, ( 1l) 2.2.4 次 级 精 母 细 胞, ( 1n), 4.50μm 3.50μm, 3.30μm 2.70μm,,, ( 1m),,,, ( 1o) 2.2.5 精 子 细 胞 ( 2a),, 3.2μm 2.6μm, 2.80μm 2.5μm( 2b),, 0.841,, ( 2c),,, ( 2d) 2.3 2.3.1 精 子 形 成 早 期,, ( 2c),, ( 2e),, ( 2f 2g) ( 2d),,,, ( 2h, 2i) 2.3.2 精 子 形 成 中 期,, ( 2j 2k), ( 2j),, ( 2k), ( 2j) 2.3.3 精 子 形 成 晚 期,, ( 2m),,,, ( 2o) 2.3.4 成 熟 精 子 结 构 3, 1.0 1.5μm,,,,, 1/2 ( 2m 2n), ( 2m), 9+2 ( 2l),, ( 2o) 3 3.1,,,, Sparus macrocephalus Rhabolosargus sarba [9],,, [9 11],,,, 魢,
: 111 2 a. ; b., ; c., ; d., ; ; e., ; f., ; g. 2e, ; h.,, ; i., ; j., ; ; k., ; ; l., ; m., 4 ; n., ; o., ; Ax: ; DC: ; ER: ; F: ; G: ; G1: ; G2: ; H: ; IF: ; LF: ; M: ; MP: ; MT: ; N: ; NU: ; NV: ; PC: ; PD: ; PS: ; PP: ; S: ; SC: ; SD: ; SE: ; SG1: ; SG2: ; SP: ; V: Fig. 2 Ultrastructural changes in Girella leonina during spermatogenesis
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