ISSN 0255-6162 GPN 2006900031 273 92 9 20 161-2 02 26531318 http://www.nlfd.gov.tw 1
SO 2 g/kg 2.0 ppm 2
(Center of Biological Evaluation and Research, FDA) 30 1991 FDA 91 10 30 Trend of Vaccine Development in the United States 3
1. (1) T (2) r-dna (3) non-ionic block copolymer (4) 2. (Institute of Medicine, IOM) 1985 14 6 1995 IOM 20 A B B FDA 1995 1985 Respiratory infection Influenza Virus A&B Cytomegalovirus Respiratory syncytial virus Group A streptococcus S. pneumoniae * M. tuberculosis Gastro-intestinal tract infection Rotavirus Shigella Enterotoxigenic E. coli H. pylori Hepatitis C virus Sexual transmitted diseases N. gonorrhea Herpes simplex virus Chlamydia Human papilloma virus Influenza Virus A&B * Cytomegalovirus Respiratory syncytial virus Group B streptococcus - - Rotavirus - - - - N. gonorrhea Herpes simplex 1&2 - - 4
1995 1985 Autoimmune & chronic disease (Therapeutic vaccine) Rheumatoid arthritis Multiple sclerosis Melanoma Alzheimer s disease Insulin-dependent diabetes Hepatitis B virus * Hepatitis A virus * Haemophilus b * Varicella-Zoster * Acellular Pertussis * Parainfluenza C. immitis 3. (International Vaccine Institute, IVI) IVI B 4. 12 B B 4 A B 92 9 5. 5
WHO DNA heat shock protein (hsp65) antigen 85 complex (Ag85A, B, C) DNA 500 5 35 % 23 2 MSD Aventis 7 Wylth DNA pneumolysin (Ply) DNA 19A Ply 1,413 苷 471 Ply DNA Liposomes Ply DNA DNA 6
CTLs DNA B 疱 6. Edible vaccine (1) (2) HBV(HBsAg) Norwalk virus(nvcp) (3) LT-B cytokine Protective Immunity and Evaluation of Pneumococcal Conjugate Vaccines 1. 3,000 50,000 7,000,000 1,200,000 90 30 4 6B 9V 14 18C 19F 23F 7 1977 14 1983 23 2 90 b 2000 pneumolysin autolysin 7
DNA 2. 3. (1) (Antibody response) 9~10 7 ELISA IgG IgM (2) (Avidity) 0.5 M (Potassium thiocyanate) Avidity index(ai) (3) (Opsonophagocytic assay) 37 15 (PMN) 60 20 µl 50 % OP titer pneumolysin autolysin type 9V PS-Ply 64 PS-Aly 16 8 (1) in vitro ( ) Anthrone assay Lowry assay Nephelometry assay NMR ELISA (2) HPLC Chromatography (3) ELISA 8
Nephelometry assay Nephelometer 90 NMR 200 1985 14 1995 O-Acetyl group Endotoxin Endotoxin Anthrone assay Nephelometry assay NMR ELISA Nephelometry assay ELISA Lowry assay HPLC Chromatography LAL assay Immune blot Anthrone assay LAL assay 1.. 2. http://www.ivi.org. 3. http://www.cdc.gov/ncidod/hip/aresist/aresist.htm 4. http://203.65.72.83/ch/vc/publications.asp http://www.worldwidevaccines.com/pipe_line/intro.asp 9
肽 ( g) ( g) Mg 13,000 Se 1 Zn 330 Si 320 Cu 600 Sn 62 Al 140 Sr 62 As 0 Ti 7 B 37 Bi 0 Ba 31 Cd 18 Be 0 Co 0 Cr 13 Ga 0 Ge 0 Li 0 Mn 370 Pb 0 Mo 130 Sc 0 Ni 15 Tl 0 Sb 0 V 0 10
酶 酶 肽 100 100 C 2 SOD SOD 11
酶 酶 酶 苷 酶 苷 3.340 µ/ml tyrosinase mushroom 97.5% 0.1mL 3 L-tyrosine 0.08 Agar 2.0% Phenoxyethanol 0.4% 酶 100 100 Arbutin 0.3 Kojic acid 0.3 0.03% L-tyrosine 1.0mL 0.9mL McI Ivaine (ph6.8) 1.0mL 2400µ/mL tyrosinase 1.0mL mushroom 37 30 470nm O.D. 酶 12
2 (NMF) NMF (%) 40 吡 (pyrrolidone carboxylic acid, PCA) 12 12 7 NH3,,, 1.5 0.5 Na, K, Ca, Mg, PO 4, Cl 18.5,, 肽 8.5 3 (%) 8 0 0.3 (capylic acid) 10 0 1.2 (capric acid) 12 0 1.0 (lauric acid) 14 0 3.2 (myristic acid) 16 0 46.0 (palmitic acid) 16 1 0.2 18 0 3.5 (stearic acid) 18 1 14.8 (oleic acid) 18 2 28.8 (linoleic acid) 18 3 0.8 - - ( - -linolenic acid) 20 1 0.1 22 0 0.1 (docasanoic acid) Na 2 SO 4 13
酶 酶 酶 酶 酶 酶 酶 酶 酶 酶 14
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