10384 9626012 UDC 1999 7 1999 7 16 1999 1999 7 1
Kluyveromyces sp. Y-85 Saccharomyces cerevisiae E-15, E-15 10 2.98g/100ml Y-85 E-15 DNA Y-85 45 YEPD MM E-15 45 30 E-15 Y-85 Y-85 12h E-15 10 h 0.1%EDTA-, 2 w/v 30 E-15 40min,Y 85 30min 90 25 30 (w/v)peg 10mmol/L CaCl 2 30min 10 6 45 MM 227 2
4 DNA F27 10 1.0 2.5%, MnSO 4 0.1% F27 ph 35 72 h 10, ph F27 4.87g/100ml F27, 15, 1 2 3 3.1 3.2 3.3 3
3.4 3.5 3.6 F27 3.7 F27 4 5 6 7 8 4
, 1 2 3 4 5 6 C 6 H 14 O 6 182.17, CH 2 OH 1.489 / 3 H-C-OH 93-97.5 ( ),110-112 HO-C-H,,H-C-OH, H-C-OH, CH 2 OH [1,2] 60 70 CO 2 H 2 O 10 [2,3], C 2 3 25 30 5
1.2 1937 Atils Powder ICI 100 1947 [1,2] [5] 140 160 40 50Mpa [2] 1.2.1 1982 Nobe 1984 Blazer [6] [7] 1993 1994 [8] [9,10,11] 95 1.2.2 1984 Viikari Zymomonas mobilis 7g/L [12,13] Glucose-Fructose Oxioreductase, GFOR [4] 6
[14,15,16] GFOR [17,18] [19,20,21,22] Jang [23,24] 50g/L 72h, 82 [25] 1988 Chun 100g/L CSTR 0.085h -1, 29.8g/L,125h 80-85g/L [26] Kim 1992 20 0.23h -1 21.3g/L/h19.2g/L/h 150h RPBR 0.35h -1 20 900ml/h 26.0g/L/h23.4g/L/h 250h 1991 Duvnjak [27,28] (Saccharomyces cerevisiae ATCC 36859) 12% 72h 4.6% 1.3 (Helianthus tuberosus L) (Ciohorium intybus) (Taraxacum afficinethus) Dahlia pinnata) (Arctium) 70% 5,000kg [29] 2,1 (D P) 25 30 3 000 5 000 [30] [29] [31] 7
[26] [32,33,34] 1.4 60 1960 Barski [35] Okada [33] 1974 Ferenczy [36] (Geotrichum Candidum) NaCl KClCa(NO 3 ) 2 Kao [35] 1972 PEG Ca 2+ PEG 1979 Pesti [37,38] [39,40,41,42] [43,44] [33] 1953 Weibull 1957 Eddy Williomson 1958 Emerson Neurospora Crassa [45] 8
PEG 30 40 PEG 10-100mmol L CaCl 2 10 5 [33,46] 10-3 PEG [47] [48,49] [50,51] [52] [33] [55,56] 9
1.5 2000 20 [57,58] 1 3 10
2.1 2.1.1 : Kluyveromyces sp 2 Y-85 Saccharomyces cerevisiae 10 E15 203 369 2300 1 E32 M53 H68 M4 Candida utilis Schizosaccharomyces sp, 2.1.2 a b c d e f 1.0% 1.0% 2.0% 2.0% ph 0.5 1.0% KH 2 PO 4 0.15%, MgSO4 7H 2 O 0.05% ph5.0 1.0% 0.5% 0.5% KH 2 PO 4 0.2%, MgSO 4 7H 2 O 0.1% (NH 4 ) 2 SO 4 0.1% ph 2.0% 2.0% 1.0% ph 4.0% 1.0% ph g h i j 4.0% 1.0% 2.0% ph YEPD): 2.0%, 2.0%, 1.0%, 2.0%, ph (MM): 2.0%, KH 2 PO 4 0.1%, MgSO 4 7H 2 O 0.05% (NH 4 ) 2 SO 4 0.1%, 2.0%, ph6.0. (YEPDS):YEPD 17% w/v. (MMS):MM 17% w/v. 11
k l m [59] : 2.0%, 1.0%, 1.5%, NaCl 0.3%, 3.0%, 2.0%, ph. (SPM): 0.1%, 0.75% KCl 0.18%,NaAc0.8% 2.0% ph6.0 (ISM): 2.0%, 1.0%, 2.0%, ph 250ml 25ml 30 12h 500ml 50ml 30 60h 2.1.3 a b c d 0.2mol/L (PB): 0.2mol/LNaH 2 PO 4 92ml 0.2 mol/l Na 2 HPO 4 8ml 200ml ph5.8 PBS 100ml PB 17 100mlPB 0.1g EDTA 0.1ml 30 PEG MW6000,10mmol/L CaCl 2 17 115 15min e 2 2g 100mlPB 17g f g 0.45 m DNS 500ml 10gDNS (3 5 ) 20g NaOH 200g 2g 0.5g, 1000ml, 7-10 DNA A 1.5g, 100ml, 1.5ml B1.6 [60], C200mlA 0.1mlB Sigma Sigma, DNA Sigma PEG 2.1.4 THZ 82,722 800 12
2.2 2.2.1 a b c 3 5 [61] [61] Grootwassink [62] 1.0ml, 1.5ml 6.6% NaIO 4,, 15min, 0 1.5ml KNO 3, 0 10min, ml, 2.0ml 1.0% 1.0ml 2.0%, 0 15min, 2.0ml 2.0ml, 15min 520nm OD 6 100 g/ml 0.00, 0.20, 0.40, 0.60, 0.80, 1.00ml 1.00ml OD 520 g ml [63] DNA [60] :1mlDNA 2.5mlC, 30 DNA 2 0.5N 0 d 0.4mg/ml DNA 0.5mmol/L NaOH f 30 OD 650 [64] A C A 100 B C A C 100 A B C 13
g h i [55] 100 4 a b V m 3 = m m 3 2 2 [65] [66,67] 0.1ml 0.9ml (5%, ph4.0 )55 15min, DNS, (u) 1 mol/l u u OD 650 2.3 Kluyveromyces sp OD 650 Y-85 13 30 72h 2.4 [59] DNA :,,, 2.5 [64], PEG CaCl 2 2.6 3 5 2.7 24h DNA YEPD 4 14
30 12h 2.8 2.8.1 2.8.2 ph 2.9 1 4 7 10 13 15 3 3.1 1984 Liisa Viikari [12] (Zymomonas mobilis),, GFOR 1991 Z Duvnjak (Saccharomyces cerevisiae) ATCC36859 Y 85 10 16h 5 30 60h 1 15
1 Table 1. Result of sorbitol fermentation Y 85 M 4 E15 369 2300 203 1 M53 E32 H68 0.156 0.183 0.215 0.487 0.175 0.275 0.362 0.427 0.197 0.191 0.282 0.152 0.182 (g/100ml) / / / / 0.75 / / / / / / 0.41 / g/100ml E-15, 0.75g/100ml,E32 0.41g/100ml E 15 10 (w/v) 3 (w/v) 10 (v/v) 32 60h 2.98g/100ml 5ml 4000rpm 5min 3 1ml 1ml 1 2ml Y-85 E 15 3.2 [59] 3.2.1, Y-85 E-15,28 2, (SPM)21 10., 2%,32 3h, 58 8 YEPD 28 3 5 1 DNA DNA DNA Schneider DNA DNA Y-85 DNA 8.3 10-8 g/cell 4.4 10-8 g/cell E-15 DNA 6.8 10-8 g/cell, 3.5 10-8 g/cell Y-85 E-15 3.2.2 16
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