Chinese Journal of Oil Crop Sciences 2014,36( 3) : 343-348 doi: 10. 7505 /j. issn. 1007-9084. 2014. 03. 008 张勇, 叶芝兰, 杨峰 * *, 张丽君, 聂邵仙, 杨文钰 611130 / / 0. 8 0. 6 1 3 / / / / P < 0. 01 / P > 0. 01 / Pn GsPSII ΦPSⅡ / NPQ CO 2 Ci Tr PSⅡ Fv /Fm PSⅡ Fv /Fo S565. 101 A 1007-9084 2014 03-0343 - 06 Effects of different light qualities on morphological and photosynthetic physiological parameters of soybean seedlings ZHANG Yong YE Zhi - lan YANG Feng * ZHANG Li - jun NIE Shao - xian YANG Wen - yu * College of Agronomy Sichuan Agricultural University / Key Laboratory of Crop Ecophysiology and Farming System in Southwest Ministry of Agriculture Chengdu 611130 China Abstract In this study three treatments of white light red /blue ratio = 0. 8 and red /far - red ratio = 0. 6 were set for simulating effects of different light qualities on the physiology and photosynthetic characteristics of soybean seedlings in maize / soybean relay intercropping conditions. Gongxuan 1 and Guixia 3 of soybean varieties were chosen as material. The morphology and photosynthetic physiological parameters of soybean seedlings under different light quality were analyzed. The results showed that soybean seedlings height were decreased and increased in red / blue treatment and red / far - red treatment compared with respectively. And the opposite result was observed in stem diameter. Root length above ground biomass below ground biomass and shoot ratio of soybean seedlings in the red / far - red treatment were decreased significantly compared with the white and red / blue treatment P < 0. 01 but there was no difference of these paraments in white and red / blue treatment. The values of leaf area and photosynthetic pigment concentration in red / far red treatment appeared minimum and maximum respectively. In addition The maximum values of net photosynthetic rates Pn stomatal conductance Gs and actual photochemical quantum yield of PSII ΦPS Ⅱ of two soybean varieties appeared in the blue / red treatment. However there were no obvious regulation of intercellular CO 2 concentration Ci transpiration rate Tr PS Ⅱ photochemical transformation efficiency Fv / Fm PS Ⅱ potential activity Fv /Fo among different treatments. Key words Soybean Red light Far - red light Blue light Light quality Growth 2013-09-22 201203096 04310624 4060562 00109253 1991 - E - mail zhangyong7630@ sina. cn * 1958 - E - mail wenyu. yang@ 263. net 1981-3S E - mail f. yang@ sicau. edu. cn
344 2014 36 3 d - 1 / = 1. 2 / = 1. 1 1. 2 1. 2. 1 形态指标测定 1 ~ 3 25d 10 4 / 105 30 min 75 IAA 1. 2. 2 叶面积的测定 Gao 10 10 5 6 7 0. 75 1. 2. 3 光合色素测定 8 / 8 2 0. 05g Li = 4. 5 4. 5 1 3 1 663nm 645nm 470nm 3 9 13 14 1. 2. 4 光合参数测定 10 00-12 00 LI - 6400 Pn Tr Gs CO 2 Ci - 1 000mol m - 2 s - 1 CO 2 450μmol mol - 1 25 15 10 3 1. 2. 5 荧光参数测定 11 12 PAM - 2100 Walz Germany F / Fm' 1. 5h LED Fm Fo / 0. 8 0. 6 NPQ Fm / Fm' - 1 PSⅡ Fv /Fm PSⅡ Fv /Fo PSⅡ ΦPS Ⅱ = Fm' - F / Fm' 16 6 1 1. 3 1. 1 Excel2003 SPSS 17. 0 1 3 2 635nm 460nm 735nm 2. 1 / 1 / 0. 8 0. 6 12 / 12cm10cm 3 1 3 12 3 12h 92%
张 勇等 : 不同光质配比对大豆幼苗形态及光合生理参数的影响 345 104% / 3 3 / P < 0. 01 P < 0. 01 / Table 1 1 Effect of different light quality on morphology of soybean seedlings 1 Gongxuan 1 3 Guixia 3 / / Height /cm 25. 75 ± 1. 09B 21. 23 ± 0. 45C 49. 49 ± 1. 13 A 27. 07 ± 0. 33B 22. 74 ± 0. 71C 55. 20 ± 1. 32A Stem diameter /mm 2. 57 ± 0. 05A 2. 69 ± 0. 052A 2. 55 ± 0. 053 A 2. 48 ± 0. 058B 2. 77 ± 0. 057A 2. 32 ± 0. 037C Root length /cm 20. 33 ± 0. 46A 20. 00 ± 0. 79A 15. 58 ± 0. 62B 20. 17 ± 0. 49A 18. 60 ± 0. 62A 13. 70 ± 0. 55B 0. 44 ± 0. 015A Above ground biomass /g 0. 47 ± 0. 027A 0. 36 ± 0. 024B 0. 48 ± 0. 031A 0. 48 ± 0. 04A 0. 24 ± 0. 015B 0. 13 ± 0. 039A Under ground biomass /g 0. 12 ± 0. 009A 0. 034 ± 0. 004B 0. 10 ± 0. 011A 0. 11 ± 0. 01A 0. 024 ± 0. 001B Root - shoot ratio 0. 30 ± 0. 10A 0. 25 ± 0. 026A 0. 095 ± 0. 006B 0. 22 ± 0. 027A 0. 24 ± 0. 032A 0. 10 ± 0. 005B 0. 01 Note Different uppercase letters indicated significant difference at 0. 01 probability level. Same as below 2. 2 / 1 2 3 18% 8% 10% 1 / / Table 2 2 Effects of different light quality on leaf area of soybean seedlings 1 Gongxuan 1 3 Guixia 3 / / Length /cm 4. 32 ± 0. 12A 4. 23 ± 0. 15A 3. 08 ± 0. 15B 5. 08 ± 0. 10A 4. 58 ± 0. 12B 3. 38 ± 0. 15C Width /cm 3. 21 ± 0. 11A 3. 20 ± 0. 12A 3. 53 ± 0. 16A 3. 76 ± 0. 07A 3. 07 ± 0. 09B 3. 57 ± 0. 14A Leaf area /cm 2 10. 34 ± 0. 91A 10. 32 ± 0. 98A 8. 80 ± 1. 24A 14. 07 ± 0. 68A 10. 49 ± 0. 87B 9. 03 ± 0. 93B 2. 3 21. 1% 23. 9% / P > 0. 01 17 3 1 3 1 3 / 1 / Table 3 / mg g - 1 Photosynthetic pigment 3 Effects of different light quality on photosynthetic pigment contents of soybean seedlings 1 Gongxuan 1 3 Guixia 3 / / a Chla 1. 34 ± 0. 078A 1. 37 ± 0. 096A 1. 66 ± 0. 33A 1. 51 ± 0. 092B 1. 25 ± 0. 091B 2. 64 ± 0. 089A b Chlb 0. 47 ± 0. 034B 0. 44 ± 0. 070B 1. 08 ± 0. 15A 0. 62 ± 0. 038B 0. 52 ± 0. 038B 1. 06 ± 0. 034A Chl 1. 80 ± 0. 12B 1. 79 ± 0. 18B 2. 97 ± 0. 11A 2. 21 ± 0. 13B 1. 86 ± 0. 13B 3. 62 ± 0. 11A Car 0. 26 ± 0. 017A 0. 28 ± 0. 021A 0. 26 ± 0. 052A 0. 31 ± 0. 017B 0. 27 ± 0. 020B 0. 44 ± 0. 013A 2. 4 1 P > 0. 01 3 CO 2 4 CO 2
346 2014 36 3 / 3 / / 88% 100% Table 4 4 Effects of different light quality on photosynthetic parameters of soybean seedling leaves 1 Gongxuan 1 3 Guixia 3 / / Pn / μmol m - 2 s - 1 4. 63 ± 0. 74A 5. 91 ± 0. 93A 4. 55 ± 0. 29A 4. 06 ± 0. 37B 7. 15 ± 0. 5A 3. 57 ± 0. 21B Gs / μmol m - 2 s - 1 0. 085 ± 0. 011A 0. 090 ± 0. 014A 0. 084 ± 0. 004A 0. 058 ± 0. 004B 0. 12 ± 0. 025A 0. 076 ± 0. 006B CO 2 Ci / μmol mol - 1 285. 88 ± 14. 36A 288. 54 ± 3. 89A 291. 83 ± 5. 25A 261. 94 ± 4. 69B 270. 20 ± 15. 46AB 296. 93 ± 5. 34A Tr / μmol m - 2 s - 1 1. 88 ± 0. 22A 1. 61 ± 0. 25A 1. 72 ± 0. 047A 1. 44 ± 0. 097B 2. 22 ± 0. 36A 1. 69 ± 0. 121AB 2. 5 3 PSⅡ Fv /Fo PSⅡ 5 1 PSⅡ Fv /Fm ΦPSⅡ Fv /FoPSⅡ Fv /Fm / NPQ P < 0. 01 ΦPSⅡ / Table 5 5 Effects of different light quality on fluorescence parameter of soybean seedling leaves 1 Gongxuan 1 3 Guixia 3 / / Fv /Fm 0. 80 ± 0. 004B 0. 80 ± 0. 002B 0. 81 ± 0. 002A 0. 80 ± 0. 001A 0. 80 ± 0. 009A 0. 80 ± 0. 009A Fv /Fo 3. 95 ± 0. 088B 3. 96 ± 0. 054B 4. 26 ± 0. 067A 4. 12 ± 0. 037A 3. 96 ± 0. 065A 3. 95 ± 0. 061A ΦPSⅡ 0. 76 ± 0. 005A 0. 79 ± 0. 001A 0. 67 ± 0. 027B 0. 75 ± 0. 008A 0. 78 ± 0. 002A 0. 73 ± 0. 006AB NPQ 0. 14 ± 0. 021A 0. 046 ± 0. 013B 0. 11 ± 0. 031A 0. 17 ± 0. 016A 0. 041 ± 0. 010B 0. 060 ± 0. 034B 3 / 0. 6 3. 1 22 23 18 19 / Li 9 20 5 3. 2 / 0. 6 0. 8 / / / IAA 24 Bach 25 21 / 0. 8 3
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