12 6 2 0 1 6 1 2 South China Fisheries Science Vol. 12, No. 6 Dec., 2016 doi: 10. 3969/ j. issn. 2095-0780. 2016. 06. 003 1, 1, 1, 1, 2, 3 ( 1.,, 510300; 2., 116013; 3.,, 116023) : ( ARS) ( CAL) 2 ( Acanthopagrus schlegelii), 2 2 ;, 2 ARS CAL, ARS CAL 2, ARS, CAL, ARS,, ; CAL, ARS : ; ; ; ; : S 931. 9 : A : 2095-0780 - ( 2016) 06-0017 - 08 Comparative study on immersion marking with alizarin red S and calcein for black porgy Acanthopagrus schlegelii of different sizes LIU Yan 1, SUN Dianrong 1, GENG Qian 1, YANG Changping 1, ZHAO Jing 2, DUAN Yan 3 ( 1. Key Lab. of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; 2. Dalian Fisheries Research Institute, Dalian 116013, China; 3. Key Lab. of Marine Biological Resources and Ecology, Liaoning Ocean Fishery Science Research Institute, Dalian 116023, China) Abstract: We used alizarin red S ( ARS) and calcein ( CAL) to dip black porgy ( Acanthopagrus schlegelii) of two sizes by fluorescent markers technique. Acute staining results show that ARS and CAL had no significant effects on the survival rate of the juveniles of both sizes. Subsequent breeding test shows no significant effects on juveniles of both sizes. The markers effects of ARS and CAL on hard tissues of the juveniles increased with increasing dye concentration, but the markers effects of ARS were better than those of CAL. No significant difference was observed for ARS, but the marker effect of CAL of small size was better than that of big size. The marker effect of ARS of fin spines was the best, followed by scales and fins, and the worst was that of otolith. CAL marker effects were at the same level for spines, fins and scales, followed by otolith. ARS marking technology has more application and research value. Key words: Acanthopagrus schlegelii; alizarin red S; calcein; fluoresent markers technique; hard tissues : 2015-07-17; : 2016-01 -18 : ( ) ( 201303048) ; ( ) ( 2015 TS07 ) ; ( 2130135020 ) : ( 1988 - ),,,, E-mail: 365830796@ qq. com : ( 1973 - ),,,, E-mail: drsun73@ 163. com
18 12 ( Acanthopagrus schlegelii), 1. 2 [ 1 ],, ARS CAL 2, 40 60 m, ARS 3 ( 100 mg L - 1, 300 mg L - 1 500 mg L - 1 ), CAL, ;, 3 ( 50 mg L - 1 150 mg L - 1 250 mg ;, L - 1 ) 105 ( 3. 5 L - 1 ), [ 2-3 ] 24 h, 12 2 14 ( 140 cm 70 cm 120 [ 4-5 ], 2007 2008 cm) 48 h, 450 10 4 [ 6 ] ; [ 7 ] 1997 11 986 1. 3 90 3 [ 8] ( n = 30), 3 ( 140 cm 70 : 1) cm 120 cm, 80 cm,, 2), 3) 0. 038 L - 1 ), [ 9], 4), 5) ; 24. 7 28. 2, ( 20. 0 2. 0), 5. 16 5. 42 mg L - 1, ph 7. 5 8. 3, [ 10-11] 14 10, [ 12 ],, 25%,,, 0. 1 cm 0. 1 g, 60 d [ 13] ( ARS) 20 40 60 ( CAL) 2, 2 1. 4,, 10 1 1. 1 2013 ) AMG,, 2 :, ( 9. 78 0. 12) cm;, ) EVOS-xl( ) AMG ( 7. 13 0. 13) cm, LIU [ 12 ] 0 5 1 000 ( 0. 15 L - 1 ) 5 m 3 ( 250 cm 250 cm 150 cm, 80 cm), 10 d ( 1 # ), 1, 3 23. 8 25. 4, ( 20 2. 0), ( 105 ) ( 30 ),,, EVOS-fl( ( 6 ( 1) 2, [ 14 ], 2, ( 1 ) ( 10 ) ( 3 5 ) 5. 23 5. 47 mg L - 1, ph 7. 6 8. 2, ( 3 5 ) [ 1 5] 14 10, 25%,
6 : 19 Tab. 1 marker definition level 1 Marker definition level and division standard division standard 0 level 0 1 level 1 2 level 2 3 level 3 4 level 4 5 level 5 1. 5 Excel 2010 SPSS 19. 0, > 0. 05) 2. 2. 2 2 2. 1 ( 2 3) ARS 24 h ARS, 300 mg L - 1 500 mg L - 1 2 1 3 ARS 500 mg L - 1, ; CAL 48 h, 2. 2. 3 60 d ARS, 2 1. 11% ( 7 /630), CAL ; ARS CAL 2, 0( 0 /630), CAL, 2 ( P >, ARS CAL, 0. 05), ARS CAL 3 2 0 d 20 d 40 d 60 d ( P > 0. 05 ) 3. 1 2 ( 1) 2. 2, ( 3 ( 0. 63% ), ), 2, [ 9 ] [ 17] MORAN [ 18 ] [ 1 9] ( 3), ARS,, CAL [ 2 0] 2. 2. 1, ARS ; CAL 3. 2 2, CAL 50 mg L - 1 ( P <0. 01) ; CAL < 0. 05) ; ( P, ARS CAL,,, ARS 300 mg L - 1 [ 16 ] 2, CAL, 150 mg L - 1 ( P < 0. 01), 250 mg L - 1 ( P,
20 12 1 2 0 d 20 d 40 d 60 d Fig. 1 Mean body length and weight of black porgy of two sizes after ARS and CAL treatments Data were collected on the 0, 20 th, 40 th and 60 th day during the growth experiment.
6 : 21 2 24 h, 2 60 d ( ) Fig. 2 Marker effects of black porgy of two sizes detected in scale, fin ray, fin spine and otolith by batches of ARS and CAL treatments No markers were detected in the control ( vertical bars show standard error). [ 21-22 ],,, CAL( 250, mg L - 1 ), [ 23 ],, 3. 3 2 ARS,,, [ 24] [ 25 ] [ 26 ] 3. 4 2 ; CAL, [ 1 5] [ 29 ],,,, ARS [ 9 ] CAL,, :, BAER R ESCH [ 27 ] ARS, < ph, ARS 150 mg L - 1 250 mg L - 1 ARS CAL, ( 40 d ) [ 28 ],, < 2
22 12
6 : 23 Fig. 3 3 Photomicrographs of black porgy of two sizes in scale, fin ray, fin spine and otolith by batches of ARS and CAL treatments, ECKMANN [ 2 1] BAER R ESCH [ 27 ] BASHEY [ 30 ] ( Salmo trutta) ( Coregonus albula) ( Poe-, cilia reticulata),, ARS 24 h [ 31 ] ARS CAL,, : ARS,, [ 1]. [ M]. :, 1962: 499-501.
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