<4D F736F F D DD6DCF6A9D2C120C6EB2020F6CE2DBACBBAEC2DC1F52E646F63>

Size: px

Start display at page:

Download "<4D F736F F D DD6DCF6A9D2C120C6EB2020F6CE2DBACBBAEC2DC1F52E646F63>"

他俞
7 years ago
Views:

1 热带海洋学报 JOURNAL OF TROPICAL OCEANOGRAPHY 2010 年第 29 卷第 6 期 : 海洋生物学齐鑫 1 1,, 黄海 2, 周雯伊 1, 尹绍武 1, 张勇 2, 刘晓春 2, 陈国华 1, 林浩然 (1., ; 2., ) 1, 2 : (HCG), 7d ( 500U ( )kg 1 ), 6 (GtH) (T 11-KT E 2 ),, Ⅰ Ⅰ, GSI Gonadosome Index 0.17±0.06%, GtH T 11-KT, T 0.1ng( ng ml 1 ); 3,, GSI 7.0±0.03%, GtH T 11-KT ; GSI 9.57±2.1%, GtH T 11-KT 3, ;, E 2, 2.36ng ml ng ml 1, GtH,,,,,, : Anguilla marmorata; ; ; ; ; GtH ; 中图分类号 : Q492.4 文献标识码 : A 文章编号 : (2010) Variations in sex steroid hormone level and changes in ultrastructure of related cells during the artificial maturation of spermary of Anguilla marmorata QI Xin 1, HUANG Hai 1,2, ZHOU Wen-yi 1, YIN Shao-wu 1, ZHANG Yong 2, LIU Xiao-chun 2, CHEN Guo-hua 1, LIN Hao-ran 1,2 (1. College of Ocean, Hainan University, Key Laboratory of Tropical Biology Resources, Ministry of Education, Haikou , China; 2. Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University, Guangzhou , China) Abstract: Serum levels of gonadotropin (GtH), testosterone (T), 11-ketotestosterone (11-KT), and estradiol-17β (E2) were assessed by radioimmunoassay during induction of sexual maturation of male marbled eel (Anguilla marmorata) by Human Chorionic Gonadotropin (HCG) treatment. Morphological alterations of the liver and GtH cell of Anguilla marmorata were investigated by means of light and electron microscopy. The testis Gonadosome Index (GSI) was 0.17±0.06% in the control group and their serum GtH and 11-KT levels were low, the T level was lower than 0.1 ng (the range of kit was ng ml 1 ); Serum GtH, T, 11-KT levels increased rapidly in the operated group after the third injection of HCG, when the GSI of testis was 7.0±0.03%. After the spermiation, the GtH, T and 11-KT levels decreased a little, but still higher than the levels of the control group. Serum E2 levels decreased gradually as HCG treatment progressed, from 2.36 ng/ml of the control group to 0.83 ng/ml of the spermiation eels. In the hepat of the control group, abundant glycongen and little lipid droplets were obversived, the nuclear was in the middle of the cell. In contrast, in the hepat of the spermiation eel, the lipid droplets accu- 收稿日期 ; 修订日期基金项目 ( ); (2007BAD29B03); (Hjkj ); ( ) (B200701A06) 作者简介 (1983 ),,,, forever_qixin@yahoo.com.cn 通信作者 : lsslhr@mail.sysu.edu.cn

2 : 131 mulated, which lead to the closing of nuclear to the cell membrane. Key words: Anguilla marmorata; artifical reproduction; sex steroid hormone; gonadotropin; sperm; GtH cell; hepatic cells Anguilla marmorata (Osteichthyes) (Anguilliformes) (Anguillidae) (Anguilla),,, ;,,, 20 40, [1 6],,, [7 12],, GtH, GtH GnRH,,, HCG, GtH, , kg, 55 66cm,,, 100cm 60cm 50cm, 1, 22 26, 24h, GtH 1.2 (8 ) (15 ),, HCG, 500U ( )kg 1, 6 3 6, HCG 24h 1mL, 4 4h, 12000r min 1, 80,, 6, 4% Bouins, 主要性激素 (GtH E 2 T 11-KT) 水平的测定方法 GtH [10], GtH, T GtH 125 I β-, γ GtH ng ml 1, (E 2 ) (T) (RIA), E 2 2 pg ml 1 (CV) 6.5%(n=10); 8.3% (n=10) E 2, 101.5% 114% T : ng ml 1, 0.1ng ml 1 (CV) 10% (n=10); 15% (n=10) 107% 110% 11- (11-KT) (EIA), Cayman, 11-KT 8 pg ml 1, (CV) 11.4%, 27% 精子脑垂体及肝脏超显微结构的观察 :,,,, 1.5mL (, )

3 132 Vol. 29, No. 6 / Nov., 2010 : Bouin s,,, 6 8μm, HE : 0.2mol L 1 (ph7.2), 2.5% 1%,,, EP812 LKB-1 1μm, 1% nm, Reynolds, JEM-100B 1.4 ± (Mean±SD), SPSS Duncan s, P< , Ⅰ Ⅰ, Gonadosome Index GSI 0.17±0.06%( 1),, 2 4.5μm,,,, ( 2a) 3, GSI 7.0±0.03%,,,, ( 2b) 6 Ⅴ, 1 Fig. 1 Variations of GSI during artificial maturation GSI 9.57±2.1%,,, ( 2c) 2.2 GtH GtH 3, GtH, 19.41±4.23ng ml 1 (n=5); 3, GtH, 24.64± 7.63ng ml 1 (n=5); GtH, 20.83±6.02ng ml 1 (n=6), 3, 2.3 T 11-KT T 11-KT 4, T 0.1ng ml 1, 11-KT 0.097±0.15ng ml 1 (n=5); 3 T 11-KT, T 3.63±0.59ng ml 1 (n=5), 11-KT 46.85±15.43ng ml 1 (n=5); T 11-KT, T 2.16±0.42ng ml 1 (n=6), 11-KT 14.67± 12.01ng ml 1 (n=6), 2.4 E 2 E 2 5, E ±994.54pg ml 1 (n=6), 3 E ±699.48pg ml 1 (n=5),, 6 E 2, ±330.80pg ml 1 (n=6), 2.5,,,, ( 2d e f),, 9, 2, 9+2 ; 9,, 9+0,, ( 2g h) 2.6 (GtH) GtH, ;,,,,,, (

4 : a, 6 Ⅰ-Ⅰ ; b, 6 HCG ; c,, ; d, ; e ; f, ; g, 9+2 ; h, 9+0 Fig. 2 Microstructure and ultrastructure observation of both testis and sperm of marbled eel

5 134 Vol. 29, No. 6 / Nov., GtH Fig. 3 Variations of serum GtH level during artificial maturation 5 E 2 Fig. 5 Variations of serum E 2 level during artificial maturation,,, ( 6e);, ( 6f) 3 4 T 11-KT Fig. 4 Variations of serum T and 11-KT level during artificial maturation 6a) 6,, GtH,,, ( 6b) 2.7,,,,, ( 6c),,, ( 6d), HCG, Ⅰ Ⅰ, ; 3,, GtH,,,,, GtH, ;,, GtH, [13] HCG GtH, GtH, GtH, [14], T <0.1ng, 11-KT 0.097±0.15ng ml 1 (n=5); 3 T 11-KT, GtH,, GtH, GtH

6 : GtH a GtH, =1μm; b GtH, GtH ; c,, ; d,, ; e, ; f, Fig. 6 Microstructure and ultrastructure observation of both GtH cell and liver of marbled eel [15 19],, T 11-KT, ;, (T 11-KT) ; T 11-KT,, T 11-KT, T 11-KT, E 2,, 11-KT, E 2 11-KT, 11-KT E 2 GtH, GtH, GtH,,

7 136 Vol. 29, No. 6 / Nov., 2010 GtH, ; 6 GtH, GtH GtH GtH :, ; 6,,, [1] YAMAMOTO K, YAMAUCHI K. Sexual maturation of Japanese eel and production of eel larvae in the aquarium [J]. Nature 1974, 251: [2],,,. [J], 1980, 4(2): [3],,,. [J], 1980, (3): [4] OKAMURA A, YAMADA Y, HORITA T. et al. Rearing eel leptocephali (Anguilla japonica Temminck Schlegel) in a planktonkreisel [J]. Aquaculture Research, 2009, 40: [5] PEDERSEN B H. Fertilisation of eggs, rate of embryonic development and hatching following induced maturation of the European eel Anguilla anguilla [J]. Aquaculture, 2004, 237: [6],,,. [J]., 2009, 33: [7] DUFOUR S, NADINE LE-BELLE, FONTAINE Y A. Effects of steroid hormones on pituitary immunoreactive gonadotropin in European freshwater eel, Anguilla anguilla [J]. L. Gen Comp Endocrinol. 1983, 52(2): [8] DUFOUR S, NADINE LE-BELLE, BALOCHE S. Positive feedback control by the gonads on gonadotropin (GtH) and gonadoliberin (GnRH) levels in experimentally matured female silver eels, Anguilla anguilla [J]. Fish Physiol and Biochem, 1989, 7: [9],,,. IV. GtH GtH [J]., 1987, 11(4): [10],. Ⅴ: (GtH) [J]., 1994(3): [11],. : 17α, 20β-. [J], 1999, 45(3): [12],,. [J]., 2001, 22(2): [13],,. [J]., 2000, 24(2): [14]. [M]. :, 1999, [15] WOODS L C, SULLIVAN C V. Reproduction of striped bass, Morone saxatilis (Walbaum), broodstock: monitoring maturation and hormonal induction of spawning [J]. Aquaculture and Fisheries Management. 1993, 21(2): [16] MYLONAS C C, ZOHAR Y, WOODS L C, et al. Hormone profiles of captive striped bass Morone saxatilisduring spermiation and long-term enhancement of milt production [J]. J World Aquacult Soc, 1998, 29(4): [17] JACKSON L F, SULLIVAN C V. Reproduction of white perch: the annual gametogenic cycle [J]. Transactions of the American Fisheries Society, 1995, 124(4): [18] PART F S, ZANUY M, CARILLO A, et al. Seasonal changes in plasma levels of gonadal steroids of sea bass, Dicentrarchus labrax L [J]. Gen Comp Endocrinol, 1990, 78(3): [19] BERLINSKY D L, JACKSON L F, SMITH T I J, et al. The annual reproductive cycle of the white bass Morone chrysops [J]. J World Aquacult Soc, 1995, 26(3):

32期

32期中国农学通报 2014,30(32):38-42 Chinese Agricultural Science Bulletin 中草药饲料添加剂对黄鳝性激素含量的影响 1,2 1,2 1,2 2 赵庆凯, 丁炜东, 邴旭文, 王建新 ( 1 南京农业大学无锡渔业学院, 江苏无锡 214081; 2 中国水产