52 12,, ( Actinopyga mauritiana) [ 1],, [ 2],, RAMOFA- [ 3-4 ],, FIA [ 21 ] MORGAN [ 2 2], Stradbroke Island,,,,,,, [ 5-1 0] 20 80,,,, 1, 1. 1,, ( 18

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1 South China Fisheries Science Vol. 12, No. 3 Jun., 2016 doi: / j. issn ,,, ( ) : ( Holothuria scabra) ( ) ( ) ( ) ( ) ( ) 5 1) :, ;,, ;, ;, ;, 2) :, ;, ;, ;,, ;, : ; ; ; ; : S : A : ( 2016) Histological characteristics of gonadal development of Holothuria scabra from Hainan Island ZHANG Li, YANG Xueming, JIANG Linyuan, YANG Qiong, HUANG Guanghua, LI Jianbin ( Mariculture Lab. of Guangxi Academy of Fishery Sciences, Nanning , China) Abstract: The characteristics of gonadal development of Holothuria scabra from Hainan Island were investigated by histological observation. Results show that gonadal development of H. scabra from Hainan Island were divided into five stages: recovery stage ( ), growing stage ( ), mature stage ( ), partly spawned stage ( ) and spent stage ( ). The characteristics of testis development are as follows: at Stage a few spermatogonias and spermatocytes started to grow along the germinal epithelium and the germ tube wall was the thickest. At Stage the germinal epithelium became thinner and the longitudinal folds extended into the lumen. At Stage lumens packed densely with mature spermatozoas, while at Stage the germinal epithelium recovered into the original folding morphology. At Stage a few of relic spermatozoa remained in the lumen. The characteristics of ovary development are as follows: at Stage oogonias and previtellogenic oocytes started to grow along the germinal epithelium. At Stage central lumen contained numbers of vitellogenic oocytes, surrounded by oogonias and previtellogenic oocytes. At Stage the lumen densely packed with vitellogenic oocytes. At Stage the previtellogenic oocytes re-presented in germinal epithelium, and some nutritional phagocytes began to occur in lumen. At Stage a few of relic vitellogenic oocytes were still in lumen. Key words: Holothuria scabra; gonadal development; testis; ovary; histology ( Holothuria scabra),,,, : ; : : ( ) ; ( ) ; ( GXIF ) : ( ), arbily0403@ sina. com : ( ),, nnyxming@ 126. com

2 52 12,, ( Actinopyga mauritiana) [ 1],, [ 2],, RAMOFA- [ 3-4 ],, FIA [ 21 ] MORGAN [ 2 2], Stradbroke Island,,,,,,, [ 5-1 0] 20 80,,,, 1, 1. 1,, ( N, E), 1 4 m [ 11-12] 1 [ ],, 3 4 g, Bouin,, sampling month / quantity of samples 1 Tab. 1 H. scabra collected in different months / g / average body weight quantity of male / quantity of female

3 3 : Bouin H E,, 24 h,,, 0. 5 cm 3, LEI- CA RM2235, 3 m, H E,, Motic BA410 ( ) :, H E RAMOFAFIA [ 21 ] m, 21 50% 28 m,, , ( 2 - A B) ( ) :,, ( ) :,,,, m ( 2 - C) 323 m( 1 - A) ( ) :, m,, m,, m,,,, ( 1 - B C) ( ) : ( 2 - D E), ( ) :,,, m,, m,,, ( 1 - D) ( ) :, ( 2 - F) m, ( ) :, m, ( 1, - E),, m, ( 1 - F) m,, 50 m,,,, m H E 2, ( ) :,,,, ( ) :, ( 2 - G)

4 A. ( ) ; B C. ( ) ; D. ( ) ; E. ( ) ; F. ( ) ; TW. ; GE. ; SC. ; SZ. ; RS. Fig. 1 Testis of H. scabra at different stages A. recovery ( Stage ) ; B C. growing( Stage ) ; C. mature( Stage ) ; E. partly spawned( Stage ) ; F. spent( Stage ) ; TW. germ; GE. germinal epithelium; SC. spermatocytes; SZ. spermatozoa; RS. relict spermatozoa 3 ( ), 3. 1 ; ( ),,, ;, ;,,

5 3 : 55 2 A B. ( ) ; C. ( ) ; D. ( ) ; E. ; F. ( ) ; G. ( ) ; O. ; PVO. ; VO. ; GV. ; F. ; AP. ; PH. ; RO. Fig. 2 Ovary of H. scabra at different stages A B. recovery ( Stage ) ; C. growing ( Stage ) ; D. mature ( Stage ) ; E. late-vitellogenic oocyte; F. partly spawned ( Stage ) ; G. spent ( Stage ) ; O. oogonia; PVO. previtellogenic oocyte; VO. vitellogenic oocyte; GV. germinal vesicle; F. follicles; AP. animal protuberance; PH. nutritive phagocyte; RO. relict oocyte, (, ; ),,,,,, 3. 2 RAMOFAFIA [ 21 ], [ ] 3,,

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2 : 111 ( Pinctada maxima), 13, 11,,,,,, 15, 25 cm, cm, 4 5 kg 1-2],,, 3-7] 24] 25-26],,, ] min, 1, 5 m 4 m, 20 m , 9-22],,, (

2 : 111 ( Pinctada maxima), 13, 11,,,,,, 15, 25 cm, cm, 4 5 kg 1-2],,, 3-7] 24] 25-26],,, ] min, 1, 5 m 4 m, 20 m , 9-22],,, ( 12 2 2 0 1 6 4 South China Fisheries Science Vol. 12, No. 2 Apr., 2016 doi: 10. 3969/ j. issn. 2095-0780. 2016. 02. 016,,,, (,,, 510300) : ( Pinctada maxima),,,, 54. 55%, 0 0. 25 m - 2, 0 70. 31 gm - 2,