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1 6 10 Vol. 6 No Journal of Food Safety and Quality Oct., 2015 佟长青 1, 翟兴月 2, 杜影 3, 李伟 (1., ; 2., ; 3., ) 1* 摘要 : 目的 方法 40 HC 50 4, g/(kg bw), 30 d 结果 0.3 g/(kg bw) 0.9 g/(kg bw) B (P<0.05), 0.9 g/(kg bw) (HC 50 )(P<0.01), 0.6 g/(kg bw) 0.9 g/(kg bw) - (P<0.05), 0.9 g/(kg bw)(p<0.05) 结论 - 关键词 : ; ; Effects of powdered Maca (Lepidium meyenii) on immune function in mice TONG Chang-Qing 1, ZHAI Xing-Yue 2, DU Ying 3, LI Wei 1* (1. College of Food Science and Engineering, Dalian Ocean University, Dalian , China; 2. Nutrition Department, the Second Hospital of Dalian Medical University, Dalian , China; 3. Dalian Center for Disease Control and Prevention, Dalian , China) ABSTRACT: Objective To evaluate the regulation effects of powdered Lepidium meyenii (Maca) on immune function in mice. Methods Forty mice were used to conduct antibody-producing cell of spleen, total hemolytic complement assay (HC 50 assay), clearance of carbon particles, and chicken red blood cell phagocytic rate and phagocytic index assay. The 40 mice were randomly divided into 4 groups (n=10): 0, 0.3, 0.6 and 0.9 g/(kg bw) powdered Maca were orally administered once daily for 30 d. Results In comparison with the control group, 0.3 g/(kg bw) and 0.9 g/(kg bw) powdered Maca significantly increased the ability of mice B cells secreting antibodies (P<0.05), 0.9 g/(kg bw) powdered Maca significantly increased HC 50 value (P<0.01), 0.6g/(kg bw) and 0.9 g/(kg bw) powdered Maca significantly increased the ability of carbon particles clearance by mice macrophages (P<0.05), and 0.9 g/(kg bw) powdered Maca significantly enhanced the phagocytic rate and phagocytic index of chicken red blood cells in mouse peritoneal macrophages (P<0.05). Conclusion Powdered Maca can enhance the function of humoral immune and monocyte macrophage in mice. KEY WORDS: powdered Lepidium meyenii; mice; immune function 基金项目 : ( ) Fund: Supported by Doctoral Project of Dalian Ocean University ( ) * 通讯作者 :,, [email protected] *Corresponding author: LI Wei, Professor, College of Food Science and Engineering, Dalian Ocean University, No.52, Heishijiao Street, Shahekou District, Dalian , China. [email protected]

2 10, : 引言 (Lepidium meyenii Walpers) [1], [2],, [3],, [4] [5] [6] [7] [8],, 2 材料与方法 2.1 实验材料,, (SRBC) Hank s Sigma ;, 18~22 g,, 12 h, 12 h, (23±2), 40%~60%, 2.2 抗体生成细胞测定 Jerne [9] 40 4 : 3, 1, g/(kg bw), 30 d, 2 ml 2%(V:V) (SRBC) 4 d 4 d,, Hank s, 2, RPM /ml Hank s 0.5 ml 10% SRBC 50 μl 20 μl ( /ml),, 2, 37 5% CO h,, 1.5 h, 2.3 半数溶血值 (HC 50 ) 测试 d, 2 ml 2%(V:V) (SRBC) 4 d 4 d min, 4 1 h, 2000 r/min 3 min, ml, 0.5 ml 10% SRBC 1 ml,, min, 2000 r/min 3 min, 1 ml, 3 ml, 0.25 ml 10% SRBC 4 ml, 10 min 540 nm, [10] (HC 50 ): HC 50 =/SRBC 2.4 小鼠碳廓清实验 d, 0.1 ml/10(g bw) 4, 2 min 10 min 20 μl, 2 ml 0.1%Na 2 CO 3, 600 nm, α [10] : K=(lgOD 2 min -lgod 10 min )/(10 min-2 min) α=/(+) K 1/3 : K ; α 2.5 吞噬功能测定 %(V:V) 1 ml 30 min,, 2 ml, 1 ml, min,,, 1:1, 4%(V:V)Ciemsa- 3 min, [10] (%)=(/ ) 100 = /

3 统计学处理 SPSS, ( x ±s) P< 结果与分析 3.1 玛咔粉对小鼠体液免疫功能的影响 d, Jerne,, 1 1, 0.3 g/(kg bw) 0.9 g/(kg bw) 0 g/(kg bw) (P<0.05) B [11] 0.3 g/(kg bw) 0.9 g/(kg bw) B (P<0.05) 表 1 Table 1 (g/(kg bw)/d) 玛咖粉对小鼠溶血空斑数的影响 ( x ± s, n=10) Effect of powdered Maca on hemolytic plaque in mice ( x ± s, n=10) ( ) ( /10 6 ) ± ± ± ± (HC 50 ) [11], HC , 0.9 g/(kg bw) (HC 50 )(P< 0.01) 1 2 P 3.2 玛咔粉对小鼠单核 - 巨噬细胞功能的影响 - [12] g/(kg bw) 0.9 g/(kg bw) - (P<0.05) 0.9 g/(kg bw) (P<0.05) -, 0.9 g/(kg bw) - (P<0.05) 表 3 玛咖粉对小鼠单核 - 巨噬细胞碳廓清能力的影响 ( x ± s, n=10) Table 3 Effect of powdered Maca on the ability of carbon particles clearance by mice macrophages ( x ± s, n=10) (g/(kg bw)/d) ( ) α P ± ± ± ± 表 4 玛咖粉对小鼠腹腔巨噬细胞鸡红细胞吞噬率及吞噬指数的影响 ( x ± s, n=10) Table 4 Effect of powdered Maca on phagocytic rate and phagocytic index of chicken red blood cells in mice peritoneal macrophages ( x ± s, n=10) (g/(kg bw)/d) ( ) (%) P P ± ± ± ± ± ± ± ± 表 2 玛咖粉对小鼠 HC 50 的影响 ( x ±s, n=10) Table 2 Effect of powdered Maca on HC 50 in mice ( x ±s, n=10) (g/(kg bw)/d) ( ) HC 50 P ± ± ± ± 讨论,, [13] [1],, C

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