Microsoft Word _排版二校

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1 8 2 Vol. 8 No Journal of Food Safety and Quality Feb., 2017 谭正林 1*, 金国梁 1, 吴梦婷 2, 刘军 (1., ; 2., ) 2* 摘要 :,,,, 关键词 : ; ; Overview on detection methods of artificial pigments in cream TAN Zheng-Lin 1*, JIN Guo-Liang 1, WU Meng-Ting 2, LIU Jun 2* (1. Department of Cuisine and Nutrition Education, Hubei University of Economics, Wuhan , China; 2. Hubei Key Laboratory of Intelligent Robot, Wuhan Institute of Technology, Wuhan , China) ABSTRACT: As the wide use of cream in cookies, cakes, cold drinks, jelly and other food, the detection of artificial pigments in cream has become an urgent problem. This paper simply introduced the classification of cream and edible artificial pigments and the using standard in China, expounded the harm of synthetic pigments to people, and then introduced the conventional detection methods and rapid detection methods of artificial pigments. Conventional analysis methods for artificial pigments mainly included high performance liquid chromatography (HPLC), thin layer chromatography (TLC), polarographic method, spectrophotometry and capillary electrophoresis method, and rapid detection methods mainly included enzyme linked immunosorbent assay (ELISA), ultra high performance liquid chromatography method (UPLC), synthetic pigment fast detector method, rapid detection reagent kit technique, Raman spectrometry and near infrared spectral analysis technology. Finally the rapid detection techniques of artificial pigments in cream in future were prospected. KEY WORDS: cream; artificial pigments; rapid detection methods 基金项目 : ( ,) ( HBIR ) Fund: Supported by Hubei Food and Drug Administration Project ( ,) and the Foundation of Hubei Provincial Key Laboratory of Intelligent Robot (HBIR ) * 通讯作者 :,, tanzhenglin@hbue.edu.cn,, liujun@wit.edu.cn *Corresponding author: TAN Zheng-Lin, Associate Professor, Department of Cuisine and Nutrition Education, Hubei University of Economics, Wuhan , China. tanzhenglin@hbue.edu.cn LIU Jun, Associate Professor, School of Computer Science and Engineering, Wuhan Institute of Technology, Wuhan , China. liujun@wit.edu.cn

2 2, : 引言,, 80%,,,,,,,,,, 2 [1] [2], ph,, [3],,,,,,,, 2 我国列入卫生使用标准的 8 种人工色素 8 : [4], : 0.1 g/kg, 0.05 g/kg, g/kg, 0.05 g/kg, 0.05 g/kg, 0.05 g/kg, 0.05 g/kg, 0.15 g/kg 3 人工色素的危害,,,,,,, [5,6] 3.1 对儿童的危害,,,,, [7],, 7 5 [8],,,,,, 3.2 人工色素的致癌性,,, [9],, [5] 4 人工色素的常规检测方法, ; ; -,, ;, ;, ; [5] 4.1 高效液相色谱法 (high performance liquid chromatography, HPLC), [10],, 3 : (1) :,, - -,,, ph, 0.45 μm [11] ; (2) :,,,,, ph, 0.45 μm

3 470 8 [12] ; (3) :,, ph 6, 0.45 μm [13],, ng [13] - (50:50, V:V),,, [12] mg/l, 86.9%~105.7%, [14], [15] XDB-C 18, 3 ( ), 86.3%~98.8% [16] 12 ( 2R ), 12, ~0.9999, 89.1%~100.4%, RSD 1.6%~8.2%, 0.58~3.0 mg/kg, 12 Chen [17] 7, 1.51~5.0 μg/l, 7 Zhang [18] 7 Olgun [19],, 4.2 薄层色谱法,,, [20] [21],, Zahra [22], 85.7% 4.3 极谱法,, [23], [24], [25], 0.27 μg/ml, 87.5%~109.6%, [26] 23,, 4.4 分光光度法 [27],, [20] [28] 2, mg/ml mg/ml, [29],, 毛细血管电泳法,,, [30] 5, 98.5%~102.6%, [31] 92 10, 95.8%~108.7%,, 5 人工色素的快速检测方法,,, [32], -,,

4 2, : 471, 5.1 免疫学检测法,,, [33],, 5.2 超高效液相色谱法, : (1) ; (2) ; (3) ; (4), ; (5) :,, [3], 0.02 mol/l -, 254 nm 629 nm 5, 0.03~0.10 mg/l, 97.4%~107.3%, RSD 0.22%~3.20%, [34] 7, 1~200 μg/ml, 1~200 μg/ml, 1~200 μg/ml, 2~200 μg/ml, 1~200 μg/ml, 2~200 μg/ml, 2~200 μg/ml,, ~0.9999, 87%~105%, 0.5%~3.1%, 0.10 ~0.30 mg/kg, 7 [35] 9, 9 18 min, , 80.1%~98.6%, 1.8%~5.2%, 1~2 ng 5.3 合成色素快速检测仪,, [33], 92%~105%, 1%, 0.5 mg/kg, 1.0 mg/kg,,, 5.4 快速检测试剂盒技术 ph - [33] 5~10 min,, 5.5 拉曼光谱技术,,, [36-38] C=O N-H O-H, C=C C-C N-N S-S [37], [39], IR, [2] (surface enhanced Raman spectroscopy, SERS), SERS 15 min, 1 mg/l,, 近红外光谱分析技术 20 90, [40] [41]

5 472 8 [42] C-H O-H N-H S-H,,, [43], ;, ;,, Karl [44],, [45], [46] [47] ; [37] ;, [48] Errington [49],,, 6 结语,,,,,,,,,, 参考文献 [1]. [J]., 2012, 1: Zhao F. Studies on edible synthetic pigment and its detection technology [J]. Food Saf Guide, 2012, 1: [2],,,. [J]. ( ), 2016, 55(5): Chen QZ, Zeng YM, Lin HZ, et a1. Developing on-site, quick screening platform for artificial pigments in food using surface-enhanced Raman spectroscopy [J]. J Xiamen Univ (Nat Sci), 2016, 55(5): [3],,,. 5 [J]., 2011, 32(4): Zhang W, Wang T, Du N, et al. Determination of five synthetic pigmentsin beverage by ultra performance liquid chromatography [J]. Food Sci, 2011, 32(4): [4]. GB [Z]. National Food Safety Risk Assessment Center. The implementation of guidelines GB National Standard for Food Safety-Standards for the use of food additives [Z]. [5],,. [J]., 2014, 27: 31. Zhang XW, Zhong X, Yi WK. Application of food coloring and its harm [J]. Chem Ind Manag, 2014, 27: 31. [6] Gautam N. Food colorants and their toxicology: an overview [J]. Sunsari Tech Coll J, 2016, 2(1): [7],. [J]., 2009, 7(6): 124. Xu YX, Xiao HB. Food additives artificial dyes harm analysis [J]. Chin Foreign Med Res, 2009, 7(6): 124. [8]. [J]., 2013, 7: 31. Zhang Q. Excessive intake of artificial coloring affects children's development [J]. Home Sci Technol, 2013, 7: 31. [9] Martins N, Roriz C L, Morales P, et al. Food colorants: challenges, opportunities and current desires of agro-industries to ensure consumer expectations and regulatory practices [J]. Trends Food Sci Technol, 2016, 52: [10] GB/T [S]. GB/T Determination of synthetic colorants in foods [S]. [11]. [J]., 2010, (03): Chen L. Discussion on the method of determination of synthetic coloring

6 2, : 473 in halogen products [J]. J Profess Tech Coll, 2010, (03): [12],,,. -HPLC 8 [J]., 2010, (02): Liu YQ, Wang H, Yang HM, et al. Determination of 8 synthetic colours in cake by solid-phase extraction couple with high performance liquid chromatography [J]. Food Res Dev, 2010, (2): [13],,,. HPLC [J]., 2010, 27(12): Guo R, Bao BH, Shao X, et al. Determination of the content of lycopene in the extract of lycopene by HPLC [J]. Jiangsu Sci Technol Inf, 2010, 27(12): [14],,. [J]., 2007, 09: Chen Y, Liu SH. Determination of synthetic colour in jelly by high performance liquid chromatography [J]. Food Sci, 2007, 09: [15],,,. [J]. ( ), 2011, 47(2): Shen J, Fu X, Pan X, et al. HPLC determination of synthetic colorants in meat food [J]. Phys Chem Anal Part B: Chem Anal, 2011, 47(2): [16],,, [J]., 2012, 24(3): Xi XL, Shao SP, Xu J, et al. Simultaneous determination of 12 synthetic colorants in foods with solid-phase extracted high performance liquid chromatography [J]. Chin J Food Hyg, 2012, 24(3): [17] Chen X, Zhao Y, Shen H, et al. Fast determination of seven synthetic pigments from wine and soft drinks using magnetic dispersive solid-phase extraction followed by liquid chromatography tandem mass spectrometry [J]. J Chromatogr A, 2014, 1346: [18] Zhang Y, Zhou H, Wang Y, et al. Simultaneous determination of seven synthetic colorants in wine by dispersive micro-solid-phase extraction coupled with reversed-phase high-performance liquid chromatography [J]. J Chromatogr Sci, 2014, 53(2): [19] Olgun FO, Ozturk BD, Apak R. Determination of synthetic food colorants in powder beverage samples by on-line hplc cupric reducing antioxidant capacity (CUPRAC) assay with post-column detection [J]. Chromatographia, 2016, 79(3 4): [20]. [J]., 2006, 22(2): Qiu HD. Recent progress of food compound colorants determination [J]. Food Mach, 2006, 22(2): [21],,,. Ⅱ 2R B[J]., 2010, 29(6): Xia LY, Han YY, Kuang LH, et al. Simultaneous determination of basic orange, soap yellow, lime yellow and sunset yellow in soybean meal by thin layer chromatography scanning and simultaneous determination of acid orange II, ponceau 2R and rhodamine B [J]. Anal Lab, 2010, 29(6): [22] Zahra N, Alim-Un-Nisa I K, Fatima S, et al. Identification of synthetic food dyes in various candies [J]. Pak J Biochem Mol Biol, 2016, 49(1): [23],,,. [J]., 2009, 34(4): 3 5. Wang HQ, Zheng DW, Lin TF, et al. Research advances of application and detection methods of edible pigments in foods [J]. Food Eng, 2009, 34(4): 3 5. [24]. [J]., 2005, 15(9): Zhng YC. Single-sweep polarographic determination of edible pigments in health care products and pharmaceutical capsules [J]. Chin J Health Lab Technol, 2005, 15(9): [25],. [J]., 2011, 39(21): Li AN, He CX. Determination of lemon yellow with differential pulse polarographic [J]. Guangzhou Chem Ind, 2011, 39(21): [26],,. [J]., 2004, 25(6): Li JD, Zhao MZ, Luan GJ. Discussion of determining artificial synthesis discussion of pimgment in the food [J]. Food Res Dev, 2004, 25(6): [27],,,. [J]., 2009, 19(4): Zhou HX, Han XD, Yu JQ, et al. Simultaneous determination of lemon yellow and sunset yellow by UV spectrophotometry [J]. Inspect Quarant Sci, 2009, 19(4): [28],,,. [J]., 2007, 24(3): Liu L, Li JQ, Guo F, et al. Ultraviolet spectrophotometry for simultaneous measurements of tartrazine and sunset yellow [J]. Chin J Spectrosc Lab, 2007, 24(3): [29],,,. [J]., 2011, 33(1): Du JZ, Li QM, Lu DM, et al. Research on determination of pigment content in food by spectrophotometry [J]. Food Sci Technol, 2011, 33(1): [30],,,. 5 [J]., 2008, (4): Zhao XY, Jia L, Zhou XJ, et al. Determination of synthetic colorant food additives in sweet by capillary zone electrophoresis [J]. Mod Instrum, 2008, (4): [31],,,. 16 [J]., 2012, (07): Long WR, Cen YH, Wang XY, et al. Determination of sixteen food additives in beverage by capillary zone electrophoresis [J]. Chin J Chromatogr, 2012, (07): [32],,,. [J]., 2011, 39(2): Zhuo J, Wang J, Chen XX, et al. Apparatus and approach for rapid determination of synthetic edible pigment in food [J]. Chin J Anal Chem, 2011, 39(2): [33]. [J]., 2015, 42(3): 4 5. Chen SP. Research and applicationof rapid detection of synthetic coloring matter in food [J]. Meas Test Technol, 2015, 42(3): 4 8. [34],,,. 7 [J]., 2014, (2):

7 474 8 Yan B, Hu J, Zhang YW, et al. Rapid determination of seven synthetic pigments in puffed foods by ultra performance liquid chromatography [J]. Chin J Health Lab Technol, 2014, (2): [35],,,. - 9 [J]., 2016, 7(2): Lin F, Li T, Wang YX, et al. Simultaneous determination of 9 kinds of synthetic pigments in preserved fruit by solid phase extraction-ultra high performance liquid chromatography [J]. J Food Saf Qual, 2016, 7(2): [36],,. [J]., 2005, 17(2): Wu L, Ouyang ZH, Cao SC, et al. Research development and application oframan scattering technology [J]. Chin J Light Scatter, 2005, 17(2): [37] Meurens M, Li WJ, Foulon M, et al. Cerevisia [J]. Health Care Manag Rev, 1995, 20(3): 33. [38],,,. [J]., 2010, (11): Liu YD, Liu T, Sun XD, et al. Application of raman spectroscopy in food quality and safety detection [J]. Spectrosc Spect Anal, 2010, (11): [39],,. [M]. :, Deng QY, Liu L, Deng HM. Spectrum analysis tutorial [M]. Beijing: Science Press, applications [J]. Spectrosc Spect Anal, 2000, (2): [44],. [J]., 2006, (02): Chen LJ, Liu W. Near infrared spectroscopy and development prospects [J]. Mod Manufact Technol Equip, 2006, (2): [45],,. [J]., 2012, (04): Tan ZL,Wu MC, Fu TD. Application review of near infrared spectroscopy technology on qualityanalyzing of agricultural products [J]. Chin J Oil Crop Sci, 2012, (4): [46] Davies AMC, Williams P. Near infrared spectroscopy: the future waves 7th proc int conf near infrared spectroscopy [C]. Davies Williams Phil, 1996: [47] Puchades JJPM, Phil DAMC. Near infrared spectroscopy: the future waves, 7th proc int conf near infrared spectroscopy [J]. Davies Williams Phil, 1996: [48] Zahn T. Near infrared transmittance spectroscopy, a powerful tool for fast process and product control in breweries and malthouses [J]. Symposium Instrum Meas, 1993: [49] Errington B, Lawson G, Lewis SW, et al. Micronised egyptian blue pigment: a novel near-infrared luminescent fingerprint dusting powder [J]. Dyes Pigm, 2016, 132: ( 责任编辑 : 姚菲 ) [40] Mcclure WF, Cox WR, Lu J. Chemometrics sunny-side up [J]. Anal Chem, 1994, (10): 584A 585A. [41],,,. [J]., 2006, (3): 作者简介 Li YL, Zhuang J, Wang WR, et al. Application of NIRS analytical method to rape quality breeding [J]. Acta Agric Shanghai, 2006, (3): [42],,. [J]., 2010, 16(2): 谭正林, 副教授, 主要研究方向为食品安全快速检测 tanzhenglin@hbue.edu.cn Mu TN, Wu YT, Sun T. Application of near-infrared spectroscopy analytical technique in safety analysis on food quality and safety testing analysis [J]. Mod Instrum, 2010, 16(2): [43],,. [J]., 2000, (2): 刘军, 副教授, 主要研究方向为食品安全快速检测 liujun@wit.edu.cn Xu GF,Yuan HF, Lu WZ. Modern near-infrared spectroscopy and its

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