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1 1 193 J Chin Med 14(3): , ( ) 2,2-diphenyl-1-picrylhydrazyl DPPH 78 µg/ml DPPH 0.1 µg/ml Vitamin C DPPH 8 mg/ml Vitamin E ICR [email protected]

2 % 41 % 29 % 113 % 3 17 β-estrodiol A C E Auricularia auricula Glycine max Fructus Ziziphi Jujubae ICR ± 1 C 60 ± 5 %

3 195 DPPH (2,2-diphenyl-1-picrylhydrazyl), Sigma Chemical Co., USA; 1,2-diacyl-sn-glycero-3-phosphocholine, Sigma Chemical Co., USA; 2-thiobarbituric acid (TBA), Sigma Chemical Co., USA, 0.7 % in NaOH solution; butylated hydroxytoluene (BHT), Sigma Chemical Co., USA, 2 % in ethanol; ferric chloride (FeCl 3 ), Riedl-deHaen; trichloroacetic acid (TCA), Riedl-deHaen; 17-α-ethinyl estradiol (EE), Sigma Chemical Co., USA 1,2-diacyl-sn-glycero-3-phosphocholine 10 mg/ml liposome solution 20 mm sodium phosphate buffer ph mg 17-α-ethinyl estradiol 1 ml 95 % 0.45 µg/ml 2.25 g 0.75 g 3 g 40 ml 100 C ml 1 Blois 14 DPPH 100 µl 2 Vitamin C 100 µl 0.2 mm DPPH nm DPPH scavenging effect % = 1- / ìl ddh 2 O 1 ìl 0.5 M FeCl 3 50 ìl 125 mm L-ascorbic acid 700 µl 37 C ìl 30 % TCA 50 µl 2 % BHT 250 µl 0.7 % TBA 400 ìl 95 C 50 1 mln-butanol 532 nm TBARS thiobarbituric acid reactive substances ( ) 150 ml 1 3

, USA, 2 % in ethanol; ferric chloride (FeCl 3 ), Riedl-deHaen; trichloroacetic acid (TCA), Riedl-deHaen; 17-α-ethinyl estradiol (EE), Sigma Chemical Co.

4 mg mg ICR A B 10 µg/kg 0.45 µg/ml 0.2 ml C 1 20 mg/ml 0.2 ml D mg/ml ml 10 ml/kg 15 4 / Kruskal-Wallis H test Dunnett t Test p < 0.1 ( )

5 197 DPPH scavenging effect % 100 L-Ascorbic acid C.Q. tea Concentration(mg/ml) 1 DPPH vitamin C N = 3 mean SD DPPH scavenging effect % CK tea Black bean Fructus Jujubae Wood ear Concentration (x 10-2 crude extract) 2 DPPH N = 3 mean SD TBARS Absorbance (532 nm) control Vitamin E C.Q. tea Concentration (mg/ml) 3 a-tocopherol Vitamin E vitamin E N = 3 mean SD

Black bean Fructus Jujubae Wood ear 0.0625 0.

6 198 (A) Uterus wet weight (mg) * 0 C EE 1x C.Q. 10x C.Q. (B) * * (uterus wet weight/ body weight) * C EE 1x C.Q. 10x C.Q. 4 ICR * p < 0.1 C EE 1 C.Q. 10 N = 5 mean SD 4B p < 0.1 H & E

10 0.05 0.00 C EE 1x C.Q. 10x C.Q. 4 ICR * p < 0.1 C EE 1 C.Q. 10 N = 5 mean SD 4B p < 0.

7 199 5 H & E stain 200 (A) (B) (C)1 (D)10 Ep 6 H & E stain 400 (A) (B) (C) Mit

8 A C E DPPH DPPH 517 nm DPPH DPPH DPPH DPPH 78 µg/ml % Vitamin C 171 ng/ml % DPPH DPPH Raloxifene 22 ICR

9 pp Rossouw JE, Anderson GL, Prentice RL and LaCroix AZ. Risks and benefits of estrogen plus progestin in healthy postmenopausal women: principal results from the women s health initiative randomized controlled trial. J Am Med Assoc 288: , Albertazzi P and Purdie DW. The nature and utility of phytoestrogens: a review of evidence. Maturitas 42: , Dalais FS, Rice GE and Wahlqvist ML. Effects of dietary phytoestrogens in postmenopausal women. Climacteric 1: , Kotsopoulus D, Dalais FS, Liang YL, McGrath PB and Teede HJ. The effects of soy protein contain phytoestrogens on menopausal symptoms on postmenopausal women. Climateric 316: , Anderson JW, Johnstone BM and Cook-Newell MD. Meta-analysis of the effects of soy protein intake on serum lipids. New Engl J Med 333: , Arjmandi BH, Alekel L, Hollis BW, Amin D, Stacewicz-Sapuntzakis M, Guo P and Kukreja SC. Dietary soybean prevents bone loss in an ovariectomised rat model of osteoporosis. J Nutr 126: , S, Lamartiniere CA. Protection against breast cancer with genistein: a component of soy. Am J Clin Nutr 71: 1705S Harman D. Aging: a theory based on free radical and radiation chemistry. J Gerotol 2: , Biesalski HK. Free radical theory of aging. Curr Opin Clin Nutr and Metab Care 5: 5-10, Blois MS. Antioxidant determination by the use of a stable free radical. Nature 181: , Odum J, Lefevre PA, Tittensor S, Paton D, Routledge EJ, Beresford NA, Sumpter JP and Ashby J. The rodent uterotrophic assay: critical protocol features, studies with nonyl phenols, and comparison with a yeast estrogenicity assay. Reg Toxicol Pharmacol 25: , 1997.

Albertazzi P and Purdie DW. The nature and utility of phytoestrogens: a review of evidence. Maturitas 42: 173-185, 2002. 5. Dalais FS, Rice GE and Wahlqvist ML.

10 pp Diel P, Schmidt S and Vollmer G. In vivo test systems for the quantitative and qualitive analysis of the biological activity of phytoestrogens. J Chromatogr. B. 777: , Jefferson WN, Banks EP, Clark G and Newbold RR. Assessing estrogenic activity of phytochemicals using transcriptional activation and immune mouse uterotrophic response. J Chromatogr. B. 777: , 2002.

In vivo test systems for the quantitative and qualitive analysis of the biological activity of phytoestrogens. J Chromatogr.

11 203 J Chin Med 14(3): , 2003 ANTIOXIDATIVE EFFECTS AND ESTROGENIC ACTIVITY OF CHINESE HERBS CHARMING-QUEEN TEA Shan-Yu Su 1, Chih-Hsiung Lee 2, Cheng-Wei Lai 2, Ying-Yuand Wang 2, Min-Chien Lai 4, Ming-Tsung Yu 5 and Ching-Hsaing Hsu 3 1 Department of Chinese Medicine 5 Institute for integration of Traditional Chinese and Western Medicine 3 Department of Pediatric, China Medical University Hospital, Taichung, Taiwan 4 Department of Pathology, China Medical University Taichung, Taiwan 2 GenMont Biotechnology Incorportation Tainan, Taiwan (Received 10 th June 2003, revised Ms received 28 th July 2003, accepted 14 th August 2003) Supplement of estrogens is conventional treatment for climacteric women to release postmenopausal symptoms. However, plenty of studies reviewed that traditional hormone replacement therapy induced many side effects. Our specific aim in this study is to look for Chinese herbs with estrogen-like activity. This study detected antioxidative effects and estrogenic activity of extract from Charming-Queen tea (C.Q. tea) which composed of fructus jujubea, black bean and Jew s ear. The antioxidative study showed dose dependence of C.Q. tea concentration with scavenging effect of 2,2- diphenyl-1-picrylhydrazyl (DPPH) free radicals and the protective effect of liposomes from ferric chloride. At the concentration of 78 µg/ml, C.Q. tea acted similar scavenging effect as 0.1 µg/ml Vitamin C. The DPPH scavenging activity of C.Q. tea is higher than any of the single fructus jujubea, black bean or Jew s ear. At the concentration of 8 mg/ml, C.Q. tea acts the similar liposome protective activity as Vitamin E. Estrogen-like activity was determined via uterotrophic assay. After feeding immature ICR mice with C.Q. tea, both uterine weights and body weights were measured. The results showed that ten times C.Q. tea can show an increase in uterine wet ratio. In addition, uterine biopsy showed higher concentration of C.Q. tea can increase the thickness of epithelium and the numbers of mitotic cells but lower concentration of C.Q. tea didn t. We therefore concluded that C.Q. tea have antioxidative effect and estrogen-like effect in the animal model. Key words: C.Q. tea, Antioxidation, Phytoestrogen. Correspondence to: Ching-Hsaing Hsu, Allergy, Immunology and Rheumatology section, Department of Pediatric, China Medical University Hospital, Tel: ext. 4163, Fax: , [email protected]

University Hospital, Taichung, Taiwan 4 Department of Pathology, China Medical University Taichung, Taiwan 2 GenMont Biotechnology Incorportation Tainan, Taiwan (Received 10 th June 2003, revised Ms

固本解郁法?治

固本解郁法?治 81 J Chin Med 13(2): 81-88, 2002 固本解鬱法論治中風後抑鬱症 (poststroke depression) 對照研究劉慶憲 1,2 宋永建 3 1 浙江省平湖市中醫醫院神經內科浙江, 平湖, 中國 2 上海第二醫科大學 2002 屆高級醫師班 3 上海第二醫科

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穨d193.PDF