,2018,38(8): 犃犮狋犪犅狅狋. 犅狅狉犲犪犾. 犗犮犮犻犱犲狀狋. 犛犻狀. : (2018) 犱狅犻 : /j.issn "#$% 犕狑犣犉犘 10 &' ( 1, 1, 1

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$@7 %AB $CD,E 443002;3E FGHI $J,E 443413) ) *:K $LMNE O 1 P $ Q R,S T < = > U V W X Y Z[\S]C^L_`645bp ( ) a b ` c D, RACE A B _ 1 犛犝犘犈犚犕犃犖 S] 犕狑犣犉犘 10(GenBank Y P MH037314) %/ *, 犕狑犣犉犘 10S] P1117bp,$

1 ,2018,38(8): 犃犮狋犪犅狅狋. 犅狅狉犲犪. 犗犮犮犱犲狀狋. 犛狀. : (2018) 犱狅 : /j.issn "#$% 犕狑犣犉犘 10 &' ( 1, 1, 1, 2, 3, 1 ( :; 45) ): <=>, ;2?@7 %AB $CD,E ;3E FGHI $J,E ) ) *:K $LMNE O 1 P $ Q R,S T < = > U V W X Y Z[\S]C^L_`645bp ( ) a b ` c D, RACE A B _ 1 犛犝犘犈犚犕犃犖 S] 犕狑犣犉犘 10(GenBank Y P MH037314) %/ *, 犕狑犣犉犘 10S] P1117bp, 726bp, 241 S, *+ P C1128H1760N322O383S14,*+ 26.4kD, 4.42, C2H2 W * ( MwZFP10 TSUPERMAN, N ` QALGGH C ` L(I)DLXLR(K)L α (Hh) (Ee) β X (Bt) MwZFP10 `S, `? ` MwZFP10 P, T c C <, 犕狑犣犉犘 10 C [ \, ` C [ \ [\ 犕狑犣犉犘 10 X S ] [ P ),, ( ) $ P, 犕狑犣犉犘 10S] `% ()C '0 +,-: ; ;S] ; ;XS]./ 0:Q785;Q786;Q789 1 :A 犆狅狀狀犵犪狀犱犉狌狀犮狋狅狀犐犱犲狀狋犳犮犪狋狅狀狅犳犕狑犣犉犘 10 犌犲狀犲犳狉狅犿犕犪犵狀狅犪狑狌犳犲狀犵犲狀狊狊 LIANGShuang 1,XIAO Aihua 1,MAJiang 1,CHENFaju 2,SANGZiyang 3,MA Lüyi 1 (1KeyLaboratoryforSilvicultureandConservationofMinistryofEducation,ColegeofForesty,BeijingForestryUniversity, Beijing100083,China;2ThreeGorgesUniversity,BiotechnologyResearchCenter,Yichang,Hubei443002,China;3Forestry ResearchInstituteofWufengCounty,Wufeng,Hubei443413,China) 犃犫狊狋狉犪犮狋 :Inthisstudy,thenewspecies 犕犪犵狀狅犪狑狌犳犲狀犵犲狀狊狊 JiaohongNo.1 wasselectedasthere searchmaterialin Wufeng,Hubei.ASUPERMANlikegene 犕狑犣犉犘 10(GenBankaccessionMH037314) wasclonedwith3 RACE method,basedonthe645bpcorefragmentrelatedwithfloraldevelopmentand thefragmentwasfilteredoutfromdiferentialyexpressedgenesbasedonpre LaboratorycDNAlibraryof 犕犪犵狀狅犪狑狌犳犲狀犵犲狀狊狊.Bioinformatics analysis of 犕狑犣犉犘 10 gene showed thatthefullength of 犕狑犣犉犘 10cDNA was1117bpwithanopenreadingframe (ORF)of726bpencoding241aminoacids. TheproteinmolecularformulaisC 1128H 1760N 322O 383S 14,molecularweightis26.4kDandisoelectricpointis 4.42.Theproteincontainsaconserveddomain:theC2H2 typezincfinger.comparativeanalysisfound thatmwzfp10belongstothesuperman likezincfingerprotein,withthe QALGGH inthentermi : ; : : ( )!: (1993-),!,"# $%,&' ()*+,- $ E mail:liangshuang@bjfu.edu.cn./01:,, #%23,&' 45) $ E mail:maluyi@bjfu.edu.cn

2 8V, : 犕狑犣犉犘 10S]` " * 1393 naland L(I)DLXLR(K)L inthecterminal.α helix(hh),extendedchain(ee)andβ turn(bt)consti tutearethebasicstructureoftheprotein.thetertiarystructureoftheproteinhasatypicalzincfinger spatialconfiguration.theproteinishydrophilicandmainlylocalizedinthenucleus.thesemi quantitative PCRandquantitativereal timepcrresultsshowedthat 犕狑犣犉犘 10wasexpressedinfloralorgans,leaf budsandbracts,andthepistilexpressionwashigherinfloralorgans. 犕狑犣犉犘 10transgenic 犃狉犪犫犱狅狆狊狊狋犺犪犪狀犪 ischaracterizedbyshortandsterileplants,curlingleaves,malformationofflowerorgans,etc. Thegene 犕狑犣犉犘 10playanimportantroleinthefloralgrowthanddevelopment. 犓犲狔狑狅狉犱狊 : 犕犪犵狀狅犪狑狌犳犲狀犵犲狀狊狊 ;zincfingerprotein;genecloning;floralorgan;transgenes C2H2 % () ` 'XY]+ [1] 犛犝犘犈犚犕犃犖 S] C2H2 FC` `S], N C * ` QALGGH L/IDLELRLG 犛犝犘犈犚犕犃犖 S] () %! " [2 4] ( 犃狉犪犫犱狅狆狊狊狋犺犪犪狀犪 )C,# U $ ( N 33 犛犝犘犈犚犕犃犖 S ] [5], C, 犛犝犘犈犚犕犃犖 S ] % ` 犛犝犘犈犚犕犃犖 S], &- 'S * `, - D ` C 0 () ()` ABC *,A S] +,- C[\,B S] +,?- C[\,. C S]/ +? 0 -[ \ [6] 犛犝犘犈犚犕犃犖 S ]. 1 - B S ]( 犃犘犈犜犃犔犃 3 犘犐犛犜犐犔犔犃犜犃 ) C ` [ \,. ` * [7] 犛犝犘犈犚犕犃犖 S]` 2C,+?- * % 3 *,. ND `(),45 D 6 N [8 9] 789 C: ; $ N 犃狋犣犉犘 1 犚犅犈犃狋犣犉犘 10 犃狋犣犉犘 11 犓犖犝犛犃犣 S ] 犃狋犣犉犘 1 S ] &- * `, [\ 犃狋犣犉犘 1S] * % < [10] ` 犚犅犈 S ] = ' S ` > V * C ' 0, 狉犫犲 2C,+,- ()?@,A B CD ` E,[ 犚犅犈 ,- ` [11 12] ` 犓犖犝 S ] &-, ` ()` [13] F G H I J K " 7 L CMNO K [5] ` 犘犺犛犝犘 1 S ] 犛犝犘犈犚犕犃犖 a, ` 狊狌狆犲狉犿犪狀 2 [ [14] 7 L ` 犌犿犣犉犘 1S] = K " + ( ) 8 V [ \ [15] :OI` 犌犣犉犘 S] C [\ P,Q `RS [16] [ \J ` 犕犱犛犝犘 3 S ],X S ] T U V S W XY [,:* [17] ( 犕犪犵狀狅犪狑狌犳犲狀犵犲狀狊狊 ) 2004 E ( `, P 7, Z,[, X\], ^ _` a,b#9(~12)= [18] c UV[8 N )<AB R $K" S ] ` ` $ [19 24] N 4 `, O ()abs]` $,Q _) % < C,W '`B 1. M ` O _ 'O ), '% H ` 5). ` $ 1 C _ 犛犝犘犈犚犕犃犖 S] 犕狑犣犉犘 10,. RACE(rap idamplificationofcdnaends) N K S ] CDS(codingsequence),Q. % / * N 犕狑犣犉犘 10 ` /, < PCR ` * 3`[\*,Q. XS] * $ N 犕狑犣犉犘 10 S ] ()C` 1 QR ~5 E < ) SO,* 1 ` ^ K" 86T -80 EASYspin RNA M (, 6) M ` RNA,Q NanoDrop2000(ThermoScientific, ) 1% RNA ` Re versetranscriptionsystem XY (Promega,

3 )W RNA XYD + cdna "# $ % 犕狑犣犉犘 10 & ' ( ) XY Z[\S]b) C$ ` 犕狑犣犉犘 10S]645bp 3 RACE 3 RACE MwZFP10 F1 3 RACE MwZFP10 F2([ 1) 3 FulRACE CoreSetwithPri mescript TM RTase (TaKaRa,7 )C ` RACE 犕狑犣犉犘 10 S]3 5 3 W 8, 犕狑犣犉犘 10S] "# $ % 犕狑犣犉犘 10 & : ( ; ( DNAMAN W 犕狑犣犉犘 10S ] cdna BLAST NCBI(htps://blast.ncbi.nlm. nih.gov/) c ` M * 犕狑犣犉犘 10S]` NCBIb) C BlastP (htps://blast.ncbi.nlm.nih.gov/ Blast.cgi) LM T M^+`12 SU PERMAN M, MEGA6.0 ` (NJ) S ` ( ) H M, BioEdit,W MwZFP10 * "# $ % 犕狑犣犉犘 10 < = > ( ExPASy(htp://web.expasy.org/protparam/) * MwZFP10 ` S ; Protscale(htp://web.expasy.org/protscale/) * MwZFP10 ; TMpredServer (htp:// PRED_form.html)* MwZFP10 ` ; SignalP4.1(htp:// services/signalp/) MwZFP10 `/ ; SOPMA (htp://npsa pbil.ibcp.fr/cgi bin/npsa_automat.pl?page=npsa_sopma.html) SWISS MODEL (htp://swissmodel.ex pasy.org/interactive)* MwZFP10 `,? ; WoLF PSORT (ht tps:// psort.html) 犕狑犣犉犘 10S]` NetPhos3.1 Server(htp:// Phos/) MwZFP "# $ % 犕狑犣犉犘 10 A B ( * M 1 ` ^ ` RNA,Q* K2μg RNA P* XYD + cdna. RT PCR 犕狑犣犉犘 10S] ^ C` 3[\ ; RT PCR 犕狑犣犉犘 10 S ] ` P RT MwZFP10 F RT MwZFP10 R,Q K 犃犮狋狀 S ] 0 P \, P RT Mwactin F RT Mwactin R([ 1),J P 2 犜犪狇 PCR StarMix(GenStar,6) < PCR?1 C 78 Table1 Primersequences Primer 3 RACE MwZFP10 F1 3 RACE MwZFP10 F2 MwZFP10BamHI F MwZFP10SacI R RT MwZFP10 F RT MwZFP10 R QMwZFP10 F QMwZFP10 R RT Mwactin F RT Mwactin R QMwactin F QMwactin R MwZFP10 F MwZFP10 R Sequence(5 3 ) CCGAAGACACTGGCAATGTTATGG ATCCATCGTCTTCATCCGCATCT CGGGATCCATGGTTGTTGAACTTGGTCTACTT CGAGCTCTTACTGCGGCGATGGCCGTTGTCCC TTCATTGACCCAACACGAGAAACTG CCGTCAACTGGGACTTGGGTTTCTG CGAAGACACTGGCAATGTTATGGGC GAAGATGCGGATGAAGACGATGGAT GCCGTGACCTGACAGATGCTCTTAT CAGACTCGTCATACTCCGCCTTTG AAGAACATCCCGTCCTCCTTACTG ACCGGAATCAAGCACAATACCTGT ATGGTTGTTGAACTTGGTCTACTT TTACTGCGGCGATGGCCGTTGTCCC

$8V, : 犕狑犣犉犘 10S]` " * 1395 AB(qRT PCR)* 犕狑犣犉犘 10 S ] M : `aw[ \ < 犕狑犣犉犘 10 S ] ` P QMwZFP10 F QMwZFP10 R,QK 犃犮狋狀 S ] 0 P \, P QMwactin F QMwactin R([1),J P FastStartUniver salsybr Green Master(Roche,$

4 8V, : 犕狑犣犉犘 10S]` " * 1395 AB(qRT PCR)* 犕狑犣犉犘 10 S ] M : `aw[ \ < 犕狑犣犉犘 10 S ] ` P QMwZFP10 F QMwZFP10 R,QK 犃犮狋狀 S ] 0 P \, P QMwactin F QMwactin R([1),J P FastStartUniver salsybr Green Master(Roche, ) P:95 5min 8,95 30s,58 30s,72 1min, 35 < b)* 2 -ΔΔ 犆 t 犕狑犣犉犘 10S] `aw[\ &?@DE': L pbi1210ps][\2 %[. PCR 犕狑犣犉犘 10S] ` * 犅犪犿犎 I 犛犪犮 I,78 1- \ 犅犪犿犎 I 犛犪犮 I(New England Biolabs, )W 犕狑犣犉犘 10S] PCR pbi121[\2 *,Q 8`#`S] pbi1212` 犡犫犪 I 犛犿犪 I, 35S MwZFP10 犕狑犣犉犘 10 S ] [ \2 J ` P MwZFP10BamHI F MwZFP10SacI R([1) 78. C 35S MwZFP10 X GV ,Q. 2 ` X 犆狅 0 % [25] T μg/mL! " #!` MS5$S ^L2%8, & ' "% V X ( : 5 $ S ` ( P XS]<) (_) 5$ IDLELRLG M ` SUPERMAN, N 2 " ` S ], V *,MwZFP10 PhSUP1 SlSUP SUP AtZFP11 AtZFP10 GZFP RBE ; 2 T *3,GmZFP1 AtZFP1 KNU ; 2 T 4 * 3 ( 3) MwZFP10 AtZFP10 2P,]5-.KS]P 犕狑犣犉犘犕狑犣犉犘 10GH4 KL ( ProtParam * MwZFP10 `* + C 1128H 1760N 322O 383S 14, *+ P26.4 kd, P 4.42, 6 (Ser) (Leu), * P12.4% 9.1%,Q/ S 9S 17 : 8 S 9S; *,; (Tyr131)* 47P2.156,* (Asn216)* 4 P , *, K P ( 4,A) TMTHMM * MwZFP10 ( 4,B) SignalP *, MwZFP10 C / ( 4, C), T* NetPhos* MwZFP10 C; 18 ` C5$( *22 16h,+, 20 8h) 5 $ 2 %8* M ^L` XS]<` DNA,Q #`S]` PCR XS] PCR ` P MwZFP10 F MwZFP10 R([1) 2 * 2.1 "#$% 犕狑犣犉犘 10 &' F78 ( RACE A B C N 犛犝犘犈犚犕犃犖 M S ],-. P 犕狑犣犉犘 10,Gen Bank Y P MH * [, ` 犕狑犣犉犘 10S]cDNA ;1117bp,/0 138bp`5 UTR 726bp` ORF 253bp`3 UTR, 241 S ( 1) 2.2 GH78IJF ( W ( 2), MwZFP10 ` N C * SUPERMAN ` ` :QALGGH < =>?@,YAB P polya CD,YAB2 =>?@P S 1 犕狑犣犉犘 10S]`c S Initiationcodon(ATG)andterminationcodon(TAA)are underlined,theboldletersarepolyatail,theamino acidconservedsequenceisunderlinedandbold Fig.1 Nucleotidesequenceanddeducedamino acidsequenceof 犕狑犣犉犘 10

$ＭＨ０３７３１４ＰｈＳＵＰ１ ôno ＡＢ１１７７４９ＲＢＥ ðñò ＯＡＯ９４８７４ＳＡＺ ðñò ＮＭ１１７８９ＳＳＵＰ ³ij ＢＡＨ５９４３２ＳＵＰ ðñò ＡＡＣ４９１１６ＳＵＰＥＲＭＡＮ ²³ \Ê Á {+ C １００ k ７５ l ５０ K ２ＭｗＺＦＰ１０ 4w ²³ ÁÂ ＧｍＺＦＰ１犌狀犲犿犪狓ＮＰ００１２３７７３２ＧＺＦＰ犌狅狊狊狔狆$

5 ! １３９６ " # $ % & ３８ＧｍＺＦＰ１ Uv ＮＰ００１２３７７３２ＧＺＦＰ Xys ＡＡＺ７９４７０２ＫＮＵ ðñò ＮＰ１９６９０５ＬＩＦ ôno ＢＡＢ５８８９７ＭｗＺＦＰ１０ <=>? ＭＨ０３７３１４ＰｈＳＵＰ１ ôno ＡＢ１１７７４９ＲＢＥ ðñò ＯＡＯ９４８７４ＳＡＺ ðñò ＮＭ１１７８９ＳＳＵＰ ³ij ＢＡＨ５９４３２ＳＵＰ ðñò ＡＡＣ４９１１６ＳＵＰＥＲＭＡＮ ²³ \Ê Á {+ C １００ k ７５ l ５０ K ２ＭｗＺＦＰ１０ 4w ²³ ÁÂ ＧｍＺＦＰ１犌狀犲犿犪狓ＮＰ００１２３７７３２ＧＺＦＰ犌狅狊狊狔狆狌犿犺狉狊狌狋狌犿ＡＡＺ７９４７０２ＫＮＵ犃狉犪犫犱狅狆狊狊狋犺犪犪狀犪狔犮ＮＰ１９６９０５ＬＩＦ犘犲狋狌狀犪犺狔犫狉犱犪ＢＡＢ５８８９７ＭｗＺＦＰ１０犕犪犵狀狅犪狑狌犳犲狀犵犲狀狊狊ＭＨ０３７３１４ＰｈＳＵＰ１犘犲狋狌狀犪ＡＢ１１７７４９ＲＢＥ犃狉犪犫犱狅狆狊狊狋犺犪犪狀犪ＯＡＯ９４８７４ＳＡＺ犃狉犪犫犱狅狆狊狊狋犺犪犪狀犪ＮＭ１１７８９ＳＳＵＰ犛犲狀犲犺狔犫狉犱犪犪狋犳狅犪ＢＡＨ５９４３２ＳＵＰ犃狉犪犫犱狅狆狊狊狋犺犪犪狀犪ＡＡＣ４９１１６ＴｈｂｘｎｄｈｎｖｄｑｕｎｆＳＵＰＥＲＭＡＮｋｚｎｆｎｇｐｎＣｎｖｄｐｎＢｋ１００Ｐｎｋ７５Ｂｕ５０Ｆ２Ｍｕｆｍｗｚｆｐ１０ｎｄｈｍｇｐｎｇｐｑｕｎｇｎｍｎＭＥＧＡ６０ Þþ²À ïü àà m BQë f １０００ n^}íî TG- K ３ＭｗＺＦＰ１０ ²³[+ #$ ²³ ± Á KMB ＭＥＧＡ６０ｐｐｄｎｕｈｂｙｎｎｎｇｍｈｄＮｕｍｂｎｂｎｈｎｄｂｐｇｈｂｊｍｆ１０００ｐｎｙＦ３Ｐｈｙｇｎｎｆｍｗｚｆｐ１０ｐｎｗｈｈｄｐｎｎｄｆｆｎｐｎｇｙ

6 ８ 1 2 <=>? 犕狑犣犉犘１０ 4;ÚB １３９７ＡＰ êåé ²³ñØ+ ＢＭｗＺＦＰ１０ òó ÀB KgCLopb １０１２ qh ã ＣＭｗＺＦＰ１０ G ôé Ｃ r%ütb Ｓ G ôb Ｙ qh r%ütb ＤＭｗＺＦＰ１０ ö±mü_é K ４ＭｗＺＦＰ１０ ²³ M+ B ＡＨｙｄｐｈｂｎｆｍｗｚｆｐ１０ｂｙｐＢＴｎｍｍｂｎｎｆｍｗｚｆｐ１０ｂｙｔｍｐｄｓｙｙＴｈｆｈｚｎｎ１０１２ｎｈｇｐｈｐｎｈｍｐｈｎｖｕＣＳｐｐｄｎｆｍｗｚｆｐ１０ｇｎｙｂｙｓＰ４１ＣｍｎｖｇＳｍｎｐｐｄＹｍｎｇｎｇｎｍｂｎｄｖｇＤＰｄｄｐｈｐｈｎｆＭｗＺＦＰ１０ｂｙＮＰｈ３１ＳｖｙＦ４Ｐｈｙｈｍｐｐｆｍｗｚｆｐ１０ｇｈＨｈ ËÌ Ｅ ÍÎÏ Ｂ Ð α β K ５ＭｗＺＦＰ１０ ²³ DÓ Àé ｈＨｈｍｎｘＥｍｎｘｎｄｄｎｄＢｍｎｂｕｎｐｈｈＦ５Ｓｎｄｕｕｐｄｎｆｍｗｚｆｐ１０ｐｎｇｙ Ü _ GH １４Ｓ Ü_ ３ＴｈÜ_Ç １Ｔｙ Ü_ K ４ＤＳＯＰＭＡ B ã α Ë Ì Ｈｈ Í Î Ï Ｅ Ç β Ð Ｂ À Ñ x < = >? ＭｗＺＦＰ１０ ² ³ Ò À B, s ２０３３２１９９ Ç ７４７ K ５ＳＷＩＳＳＭＯＤＥＬ B ã ＭｗＺＦＰ１０ ² ³ Ã Ô ¼ ½ ¾ Õ Ö À ¼

$1398 38 ( 6) MwZFP10 nucl:11;cyte:1;extr:1, c*b T " :, cc 2.4 犕狑犣犉犘 10MN?@O K ( * ( 7), 犕狑犣犉犘 10 ^ C [\,. [\&' C C,[ KS ] N `() 2.$ $(Ka namycin)^ L PCR 8,; 6 35 犛犕狑犣犉犘 10XS], C 6 V A Z`[ 8, % a, [\ 犕狑犣犉犘 10S] V, QJ ;M *KLM,:* =N, b LMO?, 7O Y2, : <( 8) A.$ quantitative Real timepcranalysis Fig.7 Tissueexpressionprofileof 犕狑犣犉犘 10gene A~C.XS] ;D.% ;E.XS] 2 ;F.

quantitative Real timepcranalysis Fig.7 Tissueexpressionprofileof 犕狑犣犉犘 10gene A~C.XS] ;D.% ;E.XS] 2 ;F.

7 ( 6) MwZFP10 nucl:11;cyte:1;extr:1, c*b T " :, cc 2.4 犕狑犣犉犘 10MN?@O K ( * ( 7), 犕狑犣犉犘 10 ^ C [\,. [\&' C C,[ KS ] N `() 2.5 犕狑犣犉犘 10 & ( PN * 犕狑犣犉犘 10 S ] ( ) `, N 犕狑犣犉犘 10S ] : [ \ 2,Q X * R%!(Ka namycin)^ L PCR 8,; 6 35 犛犕狑犣犉犘 10XS], C 6 V A Z`[ 8, % a, [\ 犕狑犣犉犘 10S] V, QJ ;M *KLM,:* =N, b LMO?, 7O Y2, : <( 8) A. PCR * ;B.< PCR * 7 犕狑犣犉犘 10S]` 3[\* * 6 MwZFP10? Fig.6 TertiarystructurepredictionofMwZFP10protein A.Thesemi quantitypcranalysis;b.quantitative Real timepcranalysis Fig.7 Tissueexpressionprofileof 犕狑犣犉犘 10gene A~C.XS] ;D.% ;E.XS] 2 ;F.% 2 8 X 犕狑犣犉犘 10S] % [ * A-Cshowthefloralorgansoftransgenicplant;Disthefloralorgansofthewild type;eistheplantofthetransgenic; Fistheplantofthewild type Fig.8 Phenotypicdiferencesbetweenthepositivetransgenic 犃狉犪犫犱狅狆狊狊 andwild type

8 8V, : 犕狑犣犉犘 10S]` " * N 4 `, O ()abs]` $Q _) % < C,W '`B 犛犝犘犈犚犕犃犖 S ] 犆 2 犎 2 F C ` `S], ()` C '0,K `;M N C * `,* QALGGH L/IDLELRLG M ` SUPERMAN, N " ` S ], V,]5 P`% O[ A 7 $ W( MwZFP10 SUPERMAN ` N C ` S :QALGGH IDLELR LG N ` C2H2 type(qalggh) " ` S #`S] D C0,C ` ` P Q&- RT PCR * 犕狑犣犉犘 10 S ] CV [\, [ KS] ` ( ) b 犛犝犘犈犚犕犃犖 S ] () %! " [2 4] 犛犝犘犈犚犕犃犖 S] F C, N 犜犃犆 1K" 犛犃犣 ], 犛犝犘犈犚犕犃犖 S ] R `() *, [\ 犕狑犣犉犘 10S] V, QJ ;M *KLM,:* =N, b LMO?, 7O Y2, : <, [ 犛犝犘 S] ` [ \ V a [ \ 犛犝犘 S ],R2S = b LM, O :?, b Y [7] 犕狑犣犉犘 10 ` C[\. [\ ` () A 2, 犕狑犣犉犘 10 () C&- `* b, 犛犝犘犈犚犕犃犖 S] CD ` `0 `a SUPERMAN X Y ] + W ( ) K " ` R %,7 T W M ` 犛犝犘犈犚犕犃犖 S] ` $, W T % ( ) `,-ORUYVW 1: [1] TAKATSUJI H.Zinc fingertranscriptionfactorsinplants [J]. 犆犲狌犪狉牔犕狅犲犮狌犪狉犔犳犲犛犮犲狀犮犲狊,1998,54(6): [2] DINKINSRD,PFLIPSENC,THOMPSON A, 犲狋犪.Ectop icexpressionofan 犃狉犪犫犱狅狆狊狊 singlezincfingergeneinto baccoresultsindwarfplants[j]. 犘犪狀狋牔犆犲犘犺狔狊狅狅犵狔, 2002,43(7):743. [3] JIANG CJ,AONO M,TAMAOKI M, 犲狋犪.SAZ,anew SUPERMAN likeprotein,negativelyregulatesasubsetof ABA responsivegenesin 犃狉犪犫犱狅狆狊狊 [J]. 犕狅犲犮狌犪狉犌犲狀犲狋犮狊牔犌犲狀狅犿犮狊,2008,279(2):183. [4] XY,,,.SUPERMAN ` $ [J].*+),2010,8(3): ZHAIXF,JIN Y S,ZHANG W, 犲狋犪.Progressesinthe SUPERMAN likesinglezincfingerprotein[j]. 犕狅犲犮狌犪狉犘犪狀狋犅狉犲犲犱狀犵,2010,8(3): [5] ENJLBRECHT CC,SCHOOF H,BOHM S.Conservation, diversificationandexpansionofc2h2zincfingerproteinsin the 犃狉犪犫犱狅狆狊狊狋犺犪犪狀犪 genome[j]. 犅犕犆犌犲狀狅犿犮狊,2004, 5(1):39. [6] COENES,MEYEROWITZE M.Thewarofthewhorls:ge neticinteractionscontrolingflowerdevelopment[j]. 犖犪狋狌狉犲, 1991,353: [7] YUNJY,WEIGELD,LEEI.EctopicexpressionofSUPER MAN suppressesdevelopmentofpetalsand stamens[j]. 犘犪狀狋牔犆犲犘犺狔狊狅狅犵 y,2002,43(1): [8] BOWMANJL,SAKAIH,JACK T, 犲狋犪.SUPERMAN,a regulatoroffloralhomeoticgenesin 犃狉犪犫犱狅狆狊狊 [J]. 犇犲狏犲狅狆犿犲狀狋,1992,114(3):599. [9] SAKAIH,MEDRANO LJ,MEYEROWITZE M.Roleof SUPERMANinmaintaining 犃狉犪犫犱狅狆狊狊 floralwhorlbound aries[j]. 犖犪狋狌狉犲,1995,378(6553): [10] TAGUE B W,GALLANT P,GOODMAN H M.Expres sionanalysisofan 犃狉犪犫犱狅狆狊狊 C2H2zincfingerproteingene [J]. 犘犪狀狋犕狅犲犮狌犪狉犅狅狅犵狔,1996,32(5): [11] TAKEDA S, MATSUMOTO N,OKADA K.RABBIT EARS,encoding a SUPERMAN likezincfinger protein, regulatespetaldevelopmentin 犃狉犪犫犱狅狆狊狊狋犺犪犪狀犪 [J]. 犇犲狏犲狅狆犿犲狀狋,2004,131(2): [12] KRIZEK B A,LEWIS M W,FLETCHERJC.RABBIT EARSisasecond whorlrepressorofagamousthatmain tainsspatialboundariesin 犃狉犪犫犱狅狆狊狊 flowers[j]. 犘犪狀狋犑狅狌狉狀犪犳狅狉犆犲牔犕狅犲犮狌犪狉犅狅狅犵狔,2006,45 (3):

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383 20186 BuletinofSoiland WaterConservation Vol.38,No.3 Jun.,2018 M A (+ 1, ' 1, 1,B # 1, 2, 1 (1.#c/ ^.(FTU^,#c ` 315100;2. `5 TU+,,#c ` 315100) -.:[# ] ( 犛狌犪犲犱犪狊犪犾狊犪 ).UP 5 6. T U. 8 9 % X L PQ 0, G.