,2017,37(6): 犃犮狋犪犅狅狋. 犅狅狉犲犪犾. 犗犮犮犻犱犲狀狋. 犛犻狀. : (2017) 犱狅犻 : /j.issn "#$%& 6 ' ()*+ 1,2, 1, 1,

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$712100;2 @AB =,CD@A 663000), -:E +, 6 FGHIJKL( 犆犿犜犘犛 )M N1O,P QR N ST, UVDW 犜犘犛 MKL9+,KLXY O,)+,KLX Z[7\ 6 FGHIJK L,]^_`abc E 犆犿犜犘犛 1~7 3, 犆犿犜犘犛 4 犆犿犜犘犛 7E 1, 16\, _ 6 FGHIJ(TPS) ;_ 5\ 犆犿犜犘犛 KL E 2,$

1 ,2017,37(6): 犃犮狋犪犅狅狋. 犅狅狉犲犪犾. 犗犮犮犻犱犲狀狋. 犛犻狀. : (2017) 犱狅犻 : /j.issn "#$%& 6 ' ()*+ 1,2, 1, 1, 1, 1, 1 (1 <=,>$? =,CD@A ), -:E +, 6 FGHIJKL( 犆犿犜犘犛 )M N1O,P QR N ST, UVDW 犜犘犛 MKL9+,KLXY O,)+,KLX Z[7\ 6 FGHIJK L,]^_`abc E 犆犿犜犘犛 1~7 3, 犆犿犜犘犛 4 犆犿犜犘犛 7E 1, 16\, _ 6 FGHIJ(TPS) ;_ 5\ 犆犿犜犘犛 KL E 2, 2~4\ ;` 7\+, TPS, CmTPS3 TPS,_ CmTPS TPS TPP1 UDP forming ; O 3,+, TPSM I (15.90% ~57.31%); Z,CmTPS1 CmTPS2 CmTPS6Z `,_ 4\ CmTPSZ ` 6 qrt PCR, +, 犆犿犜犘犛 4E ( TPS KL; 犆犿犜犘犛 KLMO E, ` ABA R E +, 犜犘犛 KLM Z K./0:+,; 6 FGHIJ;KLM; 1 :Q786;S652 2:A 犈狓狆狉犲狊犻狅狀犃狀犪犾狔狊犻狊犪狀犱犌犲狀狅犿犲犠犻犱犲犐犱犲狀狋犻犳犻犮犪狋犻狅狀狅犳犜狉犲犺犪犾狅狊犲 6 犘犺狅狊狆犺犪狋犲犛狔狀狋犺犪狊犲犌犲狀犲犉犪犿犻犾狔犻狀犕犲犾狅狀 ( 犆狌犮狌犿犻狊犿犲犾狅犔.) YANG Yongchao 1,2,ZHANG Haifei 1,YANG Xiaozhen 1, WANGZhongyuan 1,WEIChunhua 1,ZHANG Xian 1 (1ColegeofHorticulture,NorthwestA &FUniversity,Yangling,Shaanxi712100,China;2WenshanAcademyofAgricultur alsciences,wenshan,yunnan663000,china) 犃犫狊狋狉犪犮狋 :Inordertoclarifytheinformationof 犆犿犜犘犛 genefamilyandthepaternsofresponsetoenviron mentcues,weidentifiedseven 犜犘犛 sin melongenomeinthiswork.these 犜犘犛狊 distributedonseven chromosomesanddesignated 犆犿犜犘犛 1-7.Phylogeneticanalysisindicatedthat 犆犿犜犘犛 geneswouldbe classifiedintoclassⅠandclassⅡ. 犆犿犜犘犛 4and 犆犿犜犘犛 7belongtoclassⅠwith16intronsandtheirpro teinsprobablypossesstpsactivity.theotherfive 犆犿犜犘犛 genes,containingtwotofourintrons,were classifiedintoclass Ⅱ.CmTPS3consistsofonly TPSdomain,whereastheremaining TPSscomprise TPS,TPPandUDP formingdomains.cmtpsproteinshavelowsimilarity (15.90% %)within familymembers.subcelularlocalizationpredictionindicatedthatcmtps1,cmtps2andcmtps6 were localizedtothenucleusandtheothersincytoplasm.theexpressionof 犆犿犜犘犛 genesundervariousabiotic stressesshowedthat 犆犿犜犘犛 4 mightbemaingeneencodingtpsinmelonleavesunderlowtemperature stress,whilemelon 犜犘犛 familygenesweremoresensitivetosaltstressandmightbeinvolvedinregula tionofabasignalpathway.ourresultslaythefoundationforfunctionalcharacterizationofmelon 犜犘犛 familygenesinthefuture. : ; : :!"#(CARS 26 18)!:$%&(1982-),',, ()*+,-./0 1 E mail:yangyongchaos@foxmail.com :2 3,,45, ()*+, 6789: ; E mail:zhangxian098@126.com

2 6 $%&,G:+, 6 FGHIJKL Z 犓犲狔狑狅狉犱狊 :melon;tps;genefamily;expressionanalysis Uα,α 1,1 I `, HI J : 6 FGHI J(TPS) ) UDP 6 FG I 6 FG(T6P), 6 FGFGJ (TPP) F G K I [1] O 6 R,` ( R [2 3],_ T6P 9 H R I 1!"HI,# $ %9 & (, TPS T6P H I U ' \ ( H I J, TPS [4] U 犗狊犜犘犛 1 KL ()O *.1 +0,-, -./01 3; [5] ` KL U VDW 犜犘犛 KL, 3,2 K L, [6] 6,78 9: 9 犜犘犛 KL ;, KL - [7 9] 犜犘犛 KLO - ( R1` ; 5 TPS TPS TPP,< TPS./ TPP [10 11] 犜犘犛 \M K L,= _ 犜犘犛 I Y > =?,@VD W K L X 11 \ 犜犘犛 K L [10], E 9 \ [11],$ A E 12 \ [4],< +, 犜犘犛 K L M BC D +, (,, E, E F 3GHI,J_`KL Mc E NO =, [12] N 1+,KLX PI [13],E+, 1 Q R ST U TR N S T,R V D W 犜犘犛 M K L ` +, KLXY V O, Z[7\+, 犜犘犛 KL,. 7 \ 犜犘犛 K L K L W M 1`= XY, E+,- / 1- Q0 K 1 9ST ,Z[ NantE \ ], ^ 3 ~7 _ 2 `, a XYE b28 /20,16h/8h( c/ ) ` () 7 ) K 6. Q R Hoag land a V ` +, ) V (4 ) (250mmol L -1 NaCl)1 ABA (100μmol L -1 )+ 0, ^ S T [14] + 0 0(CK) h 3 8, ;.` "# 犜犘犛 78 ()9: ; V DW TPS (AtTPS1 11)E,`+,K L X Y (htps://melonomics.net/) V Blastp O(E=0.0001), TR Pfam (htp://pfam.xfam.org/)1 NCBI CDD(htp:// W J R ExPASy(htp://web.expasy.org/ protparam/)o+, TPS V K G Y 0 G I V JRSubcelularlo calizationpredictor (htp://celo.life.nctu.edu. tw/)` V Z +, 犜犘犛 KLI `abc V,T R MapInspect V a b Z J R MEME (htp://meme suite.org/) V. 2\XY 8KL *Y [15] :(1) O &b & 75% c;(2) O 270% J R Clustal W V O, J R MEGA6.0 YQ R T A, ] Y BootstrapE1000 VDW TPS (AtTPS) ) TAIR(htps:// TPS (OsTPS)) K L X Y (htp://rice.plantbiology.msu.edu/) "# 犜犘犛 *+ ; RNA J R RNAPlantPlusReagent (c ), Q R FastQuantRT Kit (with gdaase),. ] J R Primer Premier6.0! \ Z, HI C 1 QR ABIStepOnePlus\ Z V+, TPSKL, JR '( ) SYBRPremix Ex 犜犪狆 Ⅱ E SYBRmix10μL,cDNA2μL,c 0.8μL,

3 *1 ( 犘犆犚 <=> Table1 PrimersforqRT PCR KL Gene c Forwardprimer(5 3 ) Reverseprimer(5 3 ) 犆犿犜犘犛 1 GCACATTGCCGAGCCTGTTATG TTGCCTGGGATGATCCGAAACC 犆犿犜犘犛 2 GTGGTGCGGTCAGGGTAAATCC TGCGAGCCCAGTAAGCAACATC 犆犿犜犘犛 3 CTGTGGCTGGTGTCTTGTCTGAG CAGTCTCCATTCTCGTTCCGATTCG 犆犿犜犘犛 4 GCCAATAGGTTGCCCGTCTCTG ACACCAGCCCAACCAATCCATC 犆犿犜犘犛 5 CAAGTGACGCTGGCTGGTGATT AGAAATCCAAGACGCTGACGGTTC 犆犿犜犘犛 6 AAGACACCGAGCCCTGAAGTTATCT TCACAAGAGACGAACCACTGACCTA 犆犿犜犘犛 7 GGTTGCCTGTGTCTGCTGTTAGAA CCAATCCATCTTGCCTCAAACTCCT β 犃犮狋犻狀 TGCCCAGAAGTTCTATTCCAGC CATAGTTGAACCACCACTGAGGAC ddh 2O6.4μL, +, 犃犮狋犻狀 E KL X Y:95 3min,94 30s,60 30 s,72 10s, 40\, ]^ ' QR2 -ΔΔCt T VY "# 犜犘犛 78?)@AB C U O1W,`+,KLXY Z[7\ 犜犘犛 KL,]^`abc E 犆犿犜犘犛 1~7,_ ` abc( 1) CmTPS1~7 442~935\ KG,0 G I5.64~6.46, 49.94~105.54kD Z 3 \Z `,4\Z ` 6 ( 2) 2.2 "# 犜犘犛 78?DE ) O7\+, TPS V M O. A( 2), A3 +, 犜犘犛 KLM I E 2, 1 犆犿犜犘犛 4 犆犿犜犘犛 7, 16\ _ I E 2, 2~4 \ CmTPS3 TPS,_ 6\ CmTPS TPS TPP UDP forming,_ UDP forming TPS TPP ( 3) O +, TPSKLMI V O,_ 3 I (15.90%~57.31%), 8KL *Y Z,+, TPSKLM ` U' *Y E +, 犜犘犛 KLM 犜犘犛 K LM 8,JR+, VDW TPS M A( 3) _ 3 CmTPS3 9 AtTPS5,CmTPS1 9 AtTPS7,CmTPS4 9 AtTPS1 ` VDW,OsTPS1 1 AtTPS1 TPS, CmTPS4 CmTPS79 OsTPS1 AtTPS1` \ c, CmTPS4 CmTPS7 TPS 1 +, 犜犘犛 KL`abc Fig.1 Distributionof 犆犿犜犘犛狊 onchromosomes *2 "# 犜犘犛 F Table2 BasiccharacteristicsofCmTPSproteins KL Genename Accessionnumber KGY Amino acids(aa) 0 G I Theoretical isoelectricpoint Molecular weight/kd Z Predictedsubcelularlocalization 犆犿犜犘犛 1 MELO3C Nuclear 犆犿犜犘犛 2 MELO3C Nuclear 犆犿犜犘犛 3 MELO3C Cytoplasmic 犆犿犜犘犛 4 MELO3C Cytoplasmic 犆犿犜犘犛 5 MELO3C Cytoplasmic 犆犿犜犘犛 6 MELO3C Nuclear 犆犿犜犘犛 7 MELO3C Cytoplasmic

4 6 $%&,G:+, 6 FGHIJKL Z , TPSM (A)1 KL (B) Fig.2 PhylogeneticanalysisofCmTPSproteins(A)andgenestructure(B)of 犆犿犜犘犛 s *3 "# 犜犘犛 GH I ; Table3 DomainanalysisofCmTPSproteins +, TPS CmTPSprotein CmTPS1 Domain Locationofdomain (aa) e e value Accessionofdomain TPS 60~530 0 cd03788 TPP 580~ E 79 pfam02358 UDP forming 1~832 0 PLN02205 TPS 56~539 0 cd03788 CmTPS2 TPP 589~ E 86 pfam02358 UDP forming 25~841 0 PLN02205 CmTPS3 TPS 63~ E 148 cd03788 TPS 46~ E 94 COG0380 TPS 95~560 0 cd03788 CmTPS4 TPP 595~ E 64 pfam02358 UDP forming 1~922 0 PLN03064 TPS 60~543 0 cd03788 CmTPS5 TPP 593~ E 85 pfam02358 UDP forming 1~846 0 PLN02205 TPS 61~544 0 cd03788 CmTPS6 TPP 594~ E 91 pfam02358 UDP forming 1~842 0 PLN02205 TPS 95~560 0 cd03788 CmTPS7 TPP 619~ E 61 pfam02358 UDP forming 1~932 0 PLN "# 犜犘犛 78*+JK 4 3,` 250 mmol L -1 NaCl + 0,+, 犜犘犛 MKL 0 犆犿犜犘犛 1 犆犿犜犘犛 2 犆犿犜犘犛 6, 0~12h,, 犆犿犜犘犛 1 犆犿犜犘犛 2 犆犿犜犘犛 6O E,`+012h O^c TPS J K L 犆犿犜犘犛 4 犆犿犜犘犛 7 _, `1~6hc, 5,` 4 + 0, 犆犿犜犘犛 1 犆犿犜犘犛 4 犆犿犜犘犛 5 0 c,. c c,.`24h _ O 犆犿犜犘犛 7`+01hc, ; 犆犿犜犘犛 6`0~6h, c CmTPS4 CmTPS7 TPS, ` 犆犿犜犘犛 7E ( TPS K L, 犆犿犜犘犛 4E ( TPS KL 6 3,` ABA + 0 1h, 犆犿犜犘犛 3,+, 犜犘犛 MKL = 'b c, _ 犆犿犜犘犛 1 犆犿犜犘犛 2 犆犿犜犘犛 5 犆犿犜犘犛 6 犆犿犜犘犛 7 `+01h, 犆犿犜犘犛 1 犆犿犜犘犛 2 c O TPS 犆犿犜犘犛 4 犆犿犜犘犛 7, 2 \ K L = 犆犿犜犘犛 4`+03 6h,`+01 12

5 h c 犆犿犜犘犛 7`1 12h c,_ 3\ I 3 A +Y P b,y,, AtTPS1.VDW(At1g78580);AtTPS2.VDW(At1g16980); AtTPS3.VDW(At1g17000);AtTPS4.VDW(At4g27550); AtTPS5.VDW(At4g17770);AtTPS6.VDW(At1g68020); AtTPS7.VDW(At1g06410);AtTPS8.VDW(At1g70290); AtTPS9.VDW(At1g23870);AtTPS10.VDW(At1g60140); AtTPS11.VDW(At2g18700);OsTPS1. (HM050424); OsTPS2. (HM050425);OsTPS3. (HM050426); OsTPS4. (HM050427);OsTPS5. (HM050428); OsTPS6. (HM050434);OsTPS7. (HM050429); OsTPS8. (HM050430);OsTPS9. (HM050431); OsTPS10. (HM050432);OsTPS11. (HM050433) CmTPS1~CmTPS7,U Z[ 7\ TPS 3 +, VDW TPSKLM Thenumbersinthephylogenetictreeindicatetheconfidencevalues ofthebreach.attps1. 犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪 (At1g78580); AtTPS2. 犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪 (At1g16980);AtTPS3. 犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪 (At1g17000);AtTPS4. 犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪 (At4g27550); AtTPS5. 犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪 (At4g17770);AtTPS6. 犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪 (At1g68020);AtTPS7. 犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪 (At1g06410); AtTPS8. 犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪 (At1g70290);AtTPS9. 犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪 (At1g23870);AtTPS10. 犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪 (At1g60140); AtTPS11. 犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪 (At2g18700).OsTPS1. 犗狉狔狕犪狊犪狋犻狏犪 (HM050424);OsTPS2. 犗狉狔狕犪狊犪狋犻狏犪 (HM050425);OsTPS3. 犗狉狔狕犪狊犪狋犻狏犪 (HM050426);OsTPS4. 犗狉狔狕犪狊犪狋犻狏犪 (HM050427);OsTPS5. 犗狉狔狕犪狊犪狋犻狏犪 (HM050428);OsTPS6. 犗狉狔狕犪狊犪狋犻狏犪 (HM050434); OsTPS7. 犗狉狔狕犪狊犪狋犻狏犪 (HM050429);OsTPS8. 犗狉狔狕犪狊犪狋犻狏犪 (HM050430);OsTPS9. 犗狉狔狕犪狊犪狋犻狏犪 (HM050431);OsTPS10. 犗狉狔狕犪狊犪狋犻狏犪 (HM050432);OsTPS11. 犗狉狔狕犪狊犪狋犻狏犪 (HM050433);CmTPS1 -CmTPS7,7CmTPSproteinswereindentifiedinthiswork. Fig.3 PhylogeneticanalysisoftheTPSgenesfrom melon, 犃狉犪犫犻犱狅狆狊犻狊 andrice TPS 1 T6PHI (KL,` % ( R, 犜犘犛 K L ` ; ( R ` VDW $A, 犜犘犛 KLM Z KL 1 [14,16] < `+,, 犜犘犛 K L M Z 1 B C D U TR NST,`+,KLXY Z[ 7 \ 犜犘犛 KL ` VDW 9\ 11 \ 犜犘犛 KLI, U, 3\ 犜犘犛 KL U S, `VDW 1\ 犜犘犛 KL 2KKLX, 犃狋犜犘犛 2 犃狋犜犘犛 3 K L U 犃狋犜犘犛 1 [16] ` +, K L X U'./ KKLX *Y [13], U, 犆犿犜犘犛 K L M / $ : *Y, 7\ 犆犿犜犘犛 7\ KL, ` `7\ 犜犘犛 KL [16] W,7 \ CmTPS TPS, CmTPS3,_ TPP 1 UDP forming ` U ' CmTPS3 TPP, ` TPS M [14] ` VDW,R [ S OsTPS1 AtTPS1 TPS [11,17] ) A!,CmTPS4 CmTPS7 E 1 TPS M,9 OsTPS1 AtTPS1` \ c, CmTPS4 CmTPS7 TPS 9 " # 犜犘犛 K L M 2 \ K L ( 犣犿犜犘犛 1, 犣犿犜犘犛 3) TPS [18] U,+, 犜犘犛 K L M O = +0 = ` +0, TPS KL 犆犿犜犘犛 4 犆犿犜犘犛 7`1 ~6h c, 01 T6P c `,T6P E ( $ % J 1(SnRK1), SnRK1 &H 9' % (%J [19] 2 01,( H R,< \ U' 01 ) H O * [20], T6P 1 +b ` U', % R O V,_ TPS

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6 ６l 678 q CDmno ６ pqrstuv IÎÐ １０７１ aýâeø1î ００５ >FQGµHI ÔÝ ４ CD ＴＰＳ (wuv ÚÒÓÔ}ÎÐ Ｄｎｎｏｍｕｄｃｄｃｎｄｎｃ００５ｖｈｍｂｏｗｇｎＦ４Ｅｘｐｏｎｐｏｏ犆犿犜犘犛ｎｐｏｎｏｇ５ CD 犜犘犛 uv ÉÑÒÓÔ}ÎÐ Ｆ５Ｅｘｐｏｎｐｏｏ犆犿犜犘犛ｕｎｄｏｗｍｐｕｇ６ CD 犜犘犛 uv ＡＢＡ ÒÓÔ}ÎÐ Ｆ６Ｅｘｐｏｎｐｏｏ犆犿犜犘犛ｎｐｏｎｏａｂａｍｎｇ µ}犞狏犜犘犛１ Fa;ÉÑéH ２１ ñcd ÉÑ $K } a Ý < ＡＢＡ C H " 犆犿犜犘犛７３６ ÒÓ õ l 犆犿犜犘犛７ â Î Ð 犆犿犜犘犛４ " l Î [ ２４ｈ Þ ã ñ １ [ １２ｈ û ü â 犆犿犜犘犛４ Ð â ２４ｈ Ð ÿ ５５ î K Î Ð H I Ø J ÎÐK a k Q Î Ð 犃狋犜犘犛１ ý u v h

7 O ABA =, 犃狋犜犘犛 1` ABA ( R [22],U 犆犿犜犘犛 4 I = TPS _ TPSM I ( H I H,# T6P 1 HI [11] `U = TPS K L ` 3 +0 [=, 9# `= +0 = R U TR N ST Z[ 7\+, 犜犘犛 MKL,.OKL 1` V,E +, 犜犘犛 KLM Z K LM : [1] GODDIJN OJ M,VAN DUN K.Trehalose metabolismin plants[j]. 犜狉犲狀犱狊犻狀犘犾犪狀狋犛犮犻犲狀犮犲,1999,4(8): [2] 234,5 6, 7,G. %1 6 F G [J]. 0,2014,50(3): CHENSL,PENGY,ZHOU H, 犲狋犪犾.Researchadvancesin trehalose metabolism andtrehalose 6 phosphatesignalingin plants[j]. 犘犾犪狀狋犘犺狔狊犻狅犾狅犵狔犑狅狌狉狀犪犾,2014,50(3): [3] 8 9,: ;. H I K L [J]. KLX9 R,2014,33(2): LIUJ,JIANG D G.Researchadvanceontrehalosesynthesis relatedgenesincrops[j]. 犌犲狀狅犿犻犮狊犪狀犱犃狆狆犾犻犲犱犅犻狅犾狅犵狔, 2014,33(2): [4] LUNNJE,DELORGEI,FIGUEROA C M, 犲狋犪犾.Trehalose metabolisminplants[j]. 犜犺犲犘犾犪狀狋犑狅狌狉狀犪犾,2014,79: [5] LIH W,ZANGBS,DENG X W, 犲狋犪犾.Overexpressionofthe trehalose 6 phosphatesynthase gene 犗狊犜犘犛 1 enhances abiotic stresstoleranceinrice[j]. 犘犾犪狀狋犪,2011,234(5): [6],,,G. 6 FGHIJ KL,4 [J].,2008,25(1): GUO B,HU L,HE X, 犲狋犪犾.Trehalose 6 phosphatesynthase transgenictobaccoenhancedtolerancetosaltstress[j]. 犆犺犻狀犲狊犲犅狌犾犲狋犻狀狅犳犅狅狋犪狀狔,2008,25(1): [7] AB9 HIJKLO, - [J].,2001,41(4): DAIX Y,WANG Y Q,YANGB, 犲狋犪犾.Expressionof 狅狋狊犃 geneintobaccoandimprovementstresstolerance[j]. 犃犮狋犪犕犻犮狉狅犫犻狅犾狅犵犻犮犪犛犻狀犻犮犪,2001,41(4): [8] =ZC,2AD,$UE,G. $ B9 0 HJKL - F 5 G 3 [J].,2003,36(2): WANGZZ,ZHANGSZ,YANGBP, 犲狋犪犾.Trehalosesyn thasegenetransfer mediatedby 犃犵狉狅犫犪犮狋犲狉犻狌犿狋狌犿犲犳犪犮犻犲狀狊 enhancesresistancetoosmoticstressinsugarcane[j]. 犛犮犻犲狀狋犻犪犃犵狉犻犮狌犾狋狌狉犪犛犻狀犻犮犪,2003,36(2): [9] HIJ,2$K, LM,G.*. 0 NO O 6 FGHJKL 1 KL 4. [J].,2000,42 (6): ZHAO H W,CHENYJ,HU YL, 犲狋犪犾.Constructionofatre halose 6 phosphatesynthase gene driven by drought responsive promoterandexpressionofdrought resistanceintransgenictobacco [J]. 犃犮狋犪犅狅狋犪狀犻犮犪犛犻狀犻犮犪,2000,42(6): [10] VANDESTEENEL,RAMON M, 犲狋犪犾.Asingleactivetre halose 6 Psynthase(TPS)andafamilyofputativeregulato rytps likeproteinsin 犃狉犪犫犻犱狅狆狊犻狊 [J]. 犕狅犾犲犮狌犾犪狉犘犾犪狀狋, 2010,3(2): [11] ZANGB,LIH,LIW, 犲狋犪犾.Analysisoftrehalose 6 phosphate synthase(tps)genefamilysuggeststheformationoftpscom plexesinrice[j]. 犘犾犪狀狋犕狅犾. 犅犻狅犾.,2011,6: [12] =. +, [D]. : =,2014. [13] GARCIA MASAJ,BENJAK A,SANSEVERINO W, 犲狋犪犾. Thegenomeofmelon( 犆狌犮狌犿犻狊犿犲犾狅 L.)[J]. 犘狉狅犮犲犲犱犻狀犵狊狅犳狋犺犲犖犪狋犻狅狀犪犾犃犮犪犱犲犿狔狅犳犛犮犻犲狀犮犲狊,2012,109(29): [14], <,PQ7,G., bhlh L M K L Z1 _ ` [J]. <, 2016,43(2): HEJ,GU X R,WEIC H, 犲狋犪犾.Identificationandexpres sionanalysisunderabioticstressesofthebhlhtranscription factorgenefamilyin watermelon[j]. 犃犮狋犪犎狅狉狋犻犮狌犾狋狌狉犪犲犛犻狀犻犮犪,2016,43(2): [15] MU M,LUXK, 犲狋犪犾.Genome wideidentificationandanal ysisofthestress resistancefunctionofthe 犜犘犛 (Trehalose 6 PhosphateSynthase)genefamilyincoton[J]. 犅犕犆犌犲狀犲狋犻犮狊,2016,17:54. [16] YANG H,LIU Y,WANG C, 犲狋犪犾.Molecularevolutionof trehalose 6 phosphatesynthase(tps)genefamilyin 犘狅狆狌犾狌狊, 犃狉犪犫犻犱狅狆狊犻狊 andrice[j]. 犘犔狅犛犗狀犲,2012,8:e [17] BL?ZQUEZ M A,SANTOSE,FLORESCL, 犲狋犪犾.Isola tionandmolecularcharacterizationofthe 犃狉犪犫犻犱狅狆狊犻狊犜犘犛 1 gene,encoding trehalose 6 phosphate synthase[j]. 犘犾犪狀狋犑狅狌狉狀犪犾,1998,13(5): [18] R S."# 6 FGHIJKLM ] 0 1 [D]. TUV: T,2010. [19] ZHANG Y,PRIMAVESILF,JHURREEA D, 犲狋犪犾.Inhi bitionofsnf1 relatedproteinkinase1activityandregulation ofmetabolicpathwaysbytrehalose 6 phosphate[j]. 犘犾犪狀狋犘犺狔狊犻狅犾狅犵狔,2009,149(4): [20] MULLER B,PANTIN F, 犲狋犪犾.Waterdeficitsuncouple growthfromphotosynthesis,increaseccontent,andmodify therelationshipsbetweencandgrowthinsinkorgans[j]. 犑狅狌狉狀犪犾狅犳犈狓狆犲狉犻犿犲狀狋犪犾犅狅狋犪狀狔,2011,62(6): [21] FERNANDEZO,VANDESTEENEL,FEILR, 犲狋犪犾.Tre halosemetabolismisactivateduponchilingingrapevineand might participate in 犅狌狉犽犺狅犾犱犲狉犻犪狆犺狔狋狅犳犻狉犿犪狀狊 induced chilingtolerance[j]. 犘犾犪狀狋犪,2012,236(2): [22] AVONCE N,LEYMAN B, 犲狋犪犾.The Arabidopsistreha lose 6 Psynthase 犃狋犜犘犛 1geneisaregulatorofglucose,ab scisicacid,andstresssignaling[j]. 犘犾犪狀狋犘犺狔狊犻狅犾狅犵狔,2004, 136(3): (:)

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