,2018,38(7): 犃犮狋犪犅狅狋. 犅狅狉犲犪犾. 犗犮犮犻犱犲狀狋. 犛犻狀. : (2018) 犱狅犻 : /j.issn "#$%&' ()*+,-./ 01 1,2, 1,

Transcription

1 ,2018,38(7): 犃犮狋犪犅狅狋. 犅狅狉犲犪犾. 犗犮犮犻犱犲狀狋. 犛犻狀. : (2018) 犱狅犻 : /j.issn "#$%&' ()*+,-./ 01 1,2, 1,2 (1 < 5-.AB,>550025) :CDE ( 犚狅狊犪狉狅狓犫狌狉犵犺犻 Trat.) F/G HIJKLMNO/, PQ RS / TU,V-.WXY Y ' 4 Z [ \,I ] ^ _ ` a F / G b c b A (APase) (NR) ba I (SAPase) ba I T ILJK A H JK :(1)_ `a Q 45mg L -1 U F /G, Q F/K b G a L b ;)5mg L -1 Q `a, F b b G <, _ `a 3 Q (2)b APase Lb SAPase _ `a <,b A NR 45 mg L -1 _ ` a, < 45mg L -1 `a NR ;ba Y JK _ `a < Q (3) FA P Ca MgJK _ `a <,ZnJK _ `a < ;)45mg L -1 _ `a FA N P K Mn Cu BJK, Q _ `a J K Q -. C,_ `a Q F / G H ] ;b G < ba APaseLbA SAPase ba I T JK FP Q RS W 1 P /23: ; `a;/g;hijk;/ 45)6:Q :A 犈犳犲犮狋狅犳犘犺狅狊狆犺狅狉狌狊犛狌狆狆犾狔狅狀犌狉狅狑狋犺, 犖狌狋狉犻犲狀狋犆狅狀狋犲狀狋犪狀犱犚犲犾犪狋犲犱犘犺狔狊犻狅犾狅犵狔犐狀犱犲狓犲狊狅犳犚狅狊犪狉狅狓犫狌狉犵犺犻犛犲犲犱犾犻狀犵狊 GUANJiyuan 1,2,FAN Weiguo 1,2 (1ColegeofAgriculture,Guizhou University,Guiyang550025,China;2 Guizhou FruitEngineering Technology Research Centre,Guiyang550025,China) 犃犫狊狋狉犪犮狋 :Efectofphosphorussupplyongrowth,nutrientcontentandrelatedphysiologyof 犚狅狊犪狉狅狓 犫狌狉犵犺犻 seedlingswerestudied,whichwashelpfultounderstandthecharacteristicsofadaptationtolow phosphorusstressof 犚. 狉狅狓犫狌狉犵犺犻 Trat.seedlings.Weused 犚. 狉狅狓犫狌狀犵犺犻 seedlingsof Guinong5 as materialsandusedcultivatingquartzsandandvermiculitemixedmatrixascultivatingmethod.theefect ofdiferentphosphorussupplylevelsongrowth,rootmorphologicalcharacteristics,theactivityofacid phosphatase(apase),nitratereductase(nr)inrootsandleavesandsecretedacidphosphatase(sapase) : ; : : ( NY );!"#$#%&' (% )!: (1993-),(,)*+,-./,01 / / E mail: @qq.com 6789::;,,,/,01 / / E mail:[email protected]

2 T inroots,thespeciesandcontentofsmalmolecularorganicacidsinroots,andthecontentofnutrientele mentsinplantsof 犚. 狉狅狓犫狌狉犵犺犻 seedlingswerestudied.theresultsshowedthat:(1)thegrowthof 犚. 狉狅狓犫狌狀犵犺犻 seedlingswasinhibitedwhenthesupplyinglevelofphosphoruswaslowerorabove45 mg L -1 than,withasignificantlyreducedbiomass,totalrootlength,totalrootsurfacearea,totalrootvol ume,averagediameter,totalroottipnumberof 犚. 狉狅狓犫狌狀犵犺犻 seedlings.theroot shootratio,theroot hairdensityandlengthof 犚. 狉狅狓犫狌狀犵犺犻 seedlingsreachedthemaximumat5mg L -1 phosphoruslevel, those3indexeswasdecreasedwiththeincreasingofphosphorussupplylevel.(2)theactivityofapasein rootsandleavesandsapaseinrootsof 犚. 狉狅狓犫狌狀犵犺犻 seedlingsdecreasedwiththeincreaseofphosphorus level.thecontentsofoxalicacid,tartaricacid,citricacid,malicacid,aceticacidandsuccinicacidinroots of 犚. 狉狅狓犫狌狀犵犺犻 seedlingsdecreasedwiththeincreaseofphosphoruslevel.theactivitynrinleavesand rootsof 犚. 狉狅狓犫狌狀犵犺犻 seedlingsreachedthemaximumat45 mg L -1 phosphoruslevel,theactivityof NRinleavesandrootsdecreasedobviouslywhenthephosphoruslevelwaslessthanorgreaterthanthat. (3)ThecontentsofP,Caand Mgincreasedof 犚. 狉狅狓犫狌狀犵犺犻 seedlingswiththeincreaseofphosphorus level,thecontentsofzndecreasedwiththeincreaseofphosphoruslevel.thecontentsofn,p,k,mn, CuandBof 犚. 狉狅狓犫狌狀犵犺犻 seedlingsreachedthemaximumat45mg L -1 phosphoruslevel,thecontentof theseelementsdecreasedobviously whenthephosphoruslevelwaslessthanorgreaterthanthat.the studiedshowedthattoolowortoohighphosphorussupplyhadadverseefectsongrowthandnutrientab sorptionof 犚. 狉狅狓犫狌狉犵犺犻 seedlings.theincreasedofroothairlengthanddensity,theenhancedactivity ofapaseinleavesandrootsandsapaseinroots,theincreasedcontentsofsmalmolecularorganicacidswerethe importantadaptationstrategiesforthe 犚. 狉狅狓犫狌狉犵犺犻 seedlings. 犓犲狔狑狅狉犱狊 : 犚狅狊犪狉狅狓犫狌狉犵犺犻 ;phosphoruslevel;growth;nutrientcontent;physiologicalefect ( 犚狅狊犪狉狅狓犫狌狉犵犺犻 Trat.) $ A!, ) Y < Y A JK Q [1 2],, HIRS -.]^_ `a F/G HIJKL NO/, D PQ RS TU 34A HI 1 Q RS PTU ), K!/G,_ " # /G $/ U [3 7] Q RS P$ %,$&6 bc a ' blb K G b <bc 2, A [6 11] ) /,$ & 6 ( (APase) bci (SAPase) bc I T SAPaseI K PQ RS,APase $&! ( T )*C+T _,- X [12 14],I.b/A I T SAPase$&!b/ A01 *,,!! [15] ] ^ b c I I T J K L $ [15 18], ) ba T M b I 1 ' M2 H 1,) 3 ( 犖犻犮狅狋犻犪狀犪狋犪犫犪犮狌犿 ) [19] < 4 ( 犌犾狔犮犻狀犲犿犪狓 ) [20] 5 4 ( 犞犻犵狀犪狌狀犵狌犻犮狌犾犪狋犪 ) [21] ( 犎犲狏犲犪犫狉犪狊犻犾犻犲狀狊犻狊 ) [22] 9 -. :,; ; _ `a, K, N 8 X 2 $ &! b c A (NR) NO [23] Q _ ` a (HI <, K U = >L?9 [2],)Q ( 犆犻狋狉狌狊狉犲狋犻犮狌犾犪狋犪 ) A B C D E 9 JK # [4], : R S P F I G H [24], OQ RS /G bc HI LNO/ I]D, PQ RS TUJ]KL C,MNOWXY Y ' 4H [\,-.D]^_ `a "5 /F /G bc b A APase NR LbA SAPase b A I T ILJK A H JK, )C PQ RS TU 34 HI _ P 1 QR [\ 1.1 :; NO 2016S 2017S)?@

3 7,9:_ `a F/G HIJKLMNO/ AB!U NQC "5 /F X NQ F4Z "5 V 1 W WX,W W )Y X + Z[\!U ]^_, `a ],bk QNQ F c 4 H' 2 1 Y Y, X 0.3 % HCl1 7d X ` X 4H C 30cm 45cm R H U X * C I,` X _ ` ba T ILJK X VC!, a Sigma 1.2 ( 0 NO 7 ]^_ `a, H A (P 2O 5 )J K I C L125mg L -1 3, 4, F10 S 3 10 " 5 ) Y' F ZF, FG.2 1B 3)4H' A, _ ` P1, 1/2 ]^ H P 1!U]^_ `a 4H NO4H H X Hongland Arnon [ [25] 8C'M [, bp H [26], H A X NO - 3 N NH + 4 N _, C 3 1 H A ]^,M2 H 2 HonglandL Arnon H [ X0.1 mol L -1 NaOH H 2SO 4 H ph.6.0 H A 7μmol L -1 * U, NH 4+ * H A NO - 3 N NH + 4 N 4 H c ^ 7d H 1, H XpH6.0 _ ` 4H'!U, )' A X M, X]^ H!U, " H 4H `, ' A H QR4H < 1 => 1.3.1?@AB1 )I ]^ ', B F, 12 (4, 3 ) L bc K, X _ `,)105 10min, 55, Lbc K, b,) 4 A 1 X EpsonPerfection4990Photobc I WinRHIZO (Express1000XL1.0 ) b c G a L b!u I ]^ 4H' X`, B + bc, ]^ b 0~3cm b /Gb,) OlympusBX 63 b G LW G K, b a G b,b 1cm G /Gbb b CD#CE C E D A / G b 0.2g,W X Bacber \ [27] b A APase ;W X Mclanchlan\ [28] bc SAPase ;WX_ \ [29] b A NR ^ / 4!U ?@4 CF6 *+ ]^ /Gb 4, 1.5g,! -"A, 3mL ` ##Y bc-$ %&, 10mL _B, X1mL ` '-" 2 ) _B, 3 U/, C5mL 45 ` A 15min, )(20000r min -1 _ B 20 min, K 0.45 μm )* X HPLC!U4! + :WondasilC 18,! -,-.30, / N A C mol L -1 KH 2PO 4 1 (ph 2.2),/ N B C01(! ),! K C 20μL, GC210nm, T C9, / ( 0.7 ml min -1,/ N B 3.0% T 2 3 U: U 10 mg ml -1 Y 4 1mg ml -1 4,I Y 1 5 L 2 M 2 4 T 1 10 T 4,bP O 1 U ] ^ K C 7 8,9 C : 8, GHIJKL*+ F -$]!, ;, I 4 V 0.5g,X N P K Ca Mg Fe Mn Cu Zn L B JK,!U4 N J K X \,PJK X!\, K J K X < = \,Ca Mg Fe Mn Cu ZnJK X I \,B JK

4 T X>!\ [30] 1.4 MNO ) X NO I," AWX Duncan sb \ CA P C2S2 NO a 2 2I 2.1 #$%&',?@AB -., D _ `a! F /G, Q _ `a F /G$/ U 1 1, F)]^ `a c /G MA,)45mg L -1 _ `a, F GE, ' / K <; _ `a Q, F /G. U, ' / K ;) 65mg L -1 `a 3 A, /G 5mg L -1 Q `a 1, _ `a F/G U Q RS J ^, 1, F b ) 5 mg L -1 _ `a <,; <_ `a : E 2,)45 mg L -1 _ `a, Fb G a b <, 2M2_ 犘 <0.05), Q _ G L *)]^_ `a AF ]^,MA,b 1 ]^ `a F/GG Fig.1 Growthstatusof 犚狅狊犪狉狅狓犫狌狉犵犺犻 seedlingsunderdiferentsupplylevelsofphosphorus P1 #$%Q' HR S T+,?UV WX Table1 Theplantheight,basediameter,biomassandroot shootratioof 犚. 狉狅狓犫狌狉犵犺犻 seedlings underdiferentlevelsofphosphorussupply _ `a LevelsofP /(mg L -1 ) Plantheight/cm ' Basediameter/cm /K Biomass/(g plant -1 ) Shoot bc Root Plant b Root shootratio ±0.55e 0.24±0.02d 0.26±0.01e 0.23±0.01e 0.49±0.02e 0.88±0.02a ±0.50c 0.35±0.01c 0.70±0.04c 0.43±0.01c 1.13±0.04c 0.61±0.04c ±0.36a 0.46±0.01a 2.44±0.06a 0.99±0.04a 3.43±0.09a 0.41±0.30g ±0.80b 0.39±0.01b 1.20±0.06b 0.56±0.03b 1.76±0.07b 0.47±0.01f ±0.42d 0.22±0.02d 0.50±0.02d 0.26±0.02d 0.76±0.03d 0.52±0.02e ±0.61f 0.20±0.01e 0.31±0.01e 0.18±0.01f 0.48±0.02e 0.58±0.02d ±0.42g 0.17±0.01f 0.19±0.01f 0.12±0.01g 0.31±0.01f 0.63±0.02b H:)^ IA]^ JK ^ Note:Diferentnormalletersinsamecolumnmeansignificantdiferenceat0.05level.Thesameasbelow.

5 7,9:_ `a F/G HIJKLMNO/ 1319 P2 WX Table2 Therootmorphologicalcharacteristicsof 犚. 狉狅狓犫狌狉犵犺犻 seedlings underdiferentlevelsofphosphorussupply _ `a LevelsofP /(mg L -1 ) G Totallength /cm Totalsurface area/cm 2 Totalvolume /cm 3 a Average diameter/mm b Totalroottipamount /(No plant -1 ) b G Roothairs length/mm b Roothairsdensity /(No cm -1 ) ±8.94e 36.94±1.83e 0.58±0.01e 0.57±0.02d ±18.62f 0.69±0.09a ±13.65a ±9.12c ±5.02c 1.63±0.02c 0.65±0.01c ±22.50d 0.61±0.08b ±22.81b ±23.07a ±12.76a 4.29±0.01a 0.94±0.02a ±38.43a 0.52±0.09c ±10.50c ±18.31b ±7.38b 2.45±0.02b 0.75±0.02b ±46.80b 0.43±0.06d ±12.22d ±7.99d 55.64±1.68d 0.82±0.02d 0.58±0.01d ±58.48c 0.33±0.03e 70.33±7.09e ±21.26e 37.20±0.99e 0.51±0.01f 0.52±0.02e ±31.02e 0.21±0.03f 56.18±2.83f ±8.10f 22.32±1.86f 0.26±0.01g 0.43±0.02f ±26.26g 0.17±0.01g 43.33±1.45g P3 #$%Q' Y?4 犃犘犪狊犲 犖犚 犛犃犘犪狊犲 D Table3 TheactivityofAPase,SAPaseandNRinleavesandrootsof 犚. 狉狅狓犫狌狉犵犺犻 seedlings underdiferentlevelsofphosphorussupply _ `a LevelsofP /(mg L -1 ) APaseactivity/( μ g g -1 min -1 ) Leaf bc Root bci SAPaseactivityinroot /( μ g g -1 min -1 ) NRactivity/( μ g g -1 min -1 ) Leaf bc Root ±0.12a 8.66±0.12a 13.90± 0.23a 0.10±0.01c 0.06±0.01f ±0.08b 7.47±0.08b 12.42±0.12b 0.16±0.01b 0.11±0.01c ±0.07c 6.69±0.07c 11.30±0.14c 0.19±0.02a 0.16±0.01a ±0.01d 5.58±0.01d 9.94±0.20d 0.17±0.01b 0.15±0.01b ±0.01e 4.59±0.01e 9.03±0.13e 0.09±0.02d 0.09±0.01d ±0.01f 3.32±0.01f 7.85±0.18f 0.07±0.01e 0.08±0.01e ±0.01g 2.15±0.01g 7.01±0.10g 0.05±0.01f 0.04±0.01g G )5 mg L -1 Q `a. <, _ `a b G 5 mg L -1 Q ` a b G I 125 mg L -1 `a 4L 7L M, Q `a b G <$ FP Q RS W P, NQ RS ao ] 1 8X 2.2 # $ % & ' 4 犃犘犪狊犲, 犖犚 犛犃犘犪狊犲 D 3,)5mg L -1 _ `a, F b A APaseL b A M2_ `a; _ `a!, Lb A APase b A SAPase, ` a ( 犘 <0. 05), Q RS F LbA APase ba SAPase, P Q RS W 1/ P ^, F ba NR 45 mg L -1 _ `a C, Q _ `a NR ( 3),_ ` a Q $ 2^*$/] 2.3 #$%&'?4 ) CF6,*+ MNO, ]^ `a F ba. Y 96 I T ( 4) MA,)5mg L -1 `a FbA, 6 I T JK., _ `a,ba 6 I T LM JK Q, Q RS F b A I T 8 X, / P2 F Q RS bc I T $ 1 c 2.4 #$%&' 4 (KL*+ )]^ `a FA H JK 5, FA N K

6 T 4!"# Table4 Thevarietiesandcontentsoforganicacidsinrootsof 犚. 狉狅狓犫狌狉犵犺犻 seedlingsunderdiferentlevelsofphosphorussupply LevelofP /(mg L -1 ) Oxalicacid Tartaricacid Citricacid Malicacid Aceticacid Succinicacid! Totalcontentoforganic acid ±20.24a ±11.18a ±14.11a ±2.05a 60.32±3.03a ±12.15a ±0.35a ±26.05b ±15.02b ±16.05b ±1.04b 47.46±2.01b ±13.06b ±0.10b ±31.09c ±10.10c ±13.09c ±1.09c 36.41±1.01c ±11.03c ±0.11c ±34.06d ±5.08d ±12.03d ±3.05d 23.37±1.01d ±9.06d ±0.03d ±27.05e ±6.06e ±15.10e ±2.05e 14.34±0.71e ±7.06e ±0.12e ±18.03f ±5.06f ±10.06f 40.56±1.02f 10.31±0.51f 70.15±3.04f ±0.22f ±21.03g ±4.04g ±9.02g 20.90±1.04g 7.28±0.30g 38.04±0.94g ±0.14g 5 $% &' "# Table5 Elementcontentsof 犚. 狉狅狓犫狌狉犵犺犻 seedlingsunderdiferentlevelsofphosphorussupply LevelofP /(mg L -1 ) "!#$%! Macroelementcontent/% &!#$%! Microelementscontent/(mg kg -1 ) N P K Ca Mg Fe Mn Cu Zn B ±0.01cd 0.15±0.01e 1.24±0.02de 1.21±0.02g 0.08±0.01e ±32.76b 48.40±7.59d 11.44±0.20e 16.69±0.26a 46.30±3.07c ±0.01b 0.19±0.02d 1.28±0.01bc 1.27±0.01f 0.11±0.01d ±37.74bc 58.87±6.53c 13.47±0.32c 15.22±0.21b 57.95±1.50ab ±0.04a 0.24±0.02c 1.32±0.02a 1.33±0.02e 0.13±0.00cd ±21.29a 85.98±4.11a 15.32±0.31a 14.32±0.31c 60.64±1.53a ±0.05b 0.27±0.02bc 1.30±0.02ab 1.38±0.01d 0.14±0.02bc ±21.54ab 68.91±4.34b 14.75±0.23b 13.76±0.28d 52.30±2.12bc ±0.01cd 0.31±0.03bc 1.26±0.01cd 1.43±0.03c 0.15±0.02abc ±19.90cd 60.93±2.01bc 12.45±0.32d 13.11±0.10e 47.29±2.95c ±0.02d 0.36±0.02a 1.22±0.02ef 1.49±0.02b 0.16±0.00ab ±28.80cd 53.79±2.80cd 10.49±0.26f 12.67±0.21f 37.10±7.38d ±0.14e 0.38±0.03a 1.20±0.03f 1.56±0.02a 0.17±0.01a ±20.23d 39.23±2.63e 9.04±0.38g 10.50±0.25g 34.44±1.04d

7 7,9:_ `a F/G HIJKLMNO/ 1321 Fe Mn Cu B,MJK 45mg L -1 `a C, Q _ `a #;;_ `a.85 mg L -1, JK #J ^, FA P Ca Mg JK _ `a <,Zn JK P _ `a < Q, FA K Fe JK)45 65 mg L -1 Q `a c FA B JK)25 45mg L -1 _ ` a c 2 _ `a c FA N P K Ca Mg Fe Mn Cu ZnL B J ` a ( 犘 <0.05) :_ " N K Fe Mn Cu B Zn J ],S M P Ca Mg!8X 3 T P Q RS : bc * bci 9 PTU,)] ^ PTU [15] b < P Q RS P,)Q RS 6 bc <,6 /G Q UV, P P 2A" bci O [31] )Q `a, Fb <, W )X" Y [4,9,32 33] $ & 6 b c * PQ RS,6 : b 'b b G L K, bc2, $% [11] b HI 1 8X, b 1~10 L [34] )M-.A,)5mg L -1 Q `a Fb G b R,Sb G F L D, bc H I M 1! 8 X, F P Q R S W 1 P ^,)]^_ `a+, Fb A Apase b A SAPase * ^, :C _ `a Q,Q RS Apase SAPase., C APase, `Q RS7 ( Apase',! Apase M,! ( T )*C+T _/G,- X [35 37],Q R S b A Apase NaO _ ] Z ] 8 X ) Q + F b A SAPase, [ b c < I K,!b/-\A T I, ] _^ Q RS F LbA APase ba SAPase, / FP Q RSW 1/ P b I NO _ [32] Q R S Q F b A NR, 2 ` a ] NR b(, NO 3-! c, NR $/, M [38],! NO 3- NH 3 L [39] NADPH,NADP + /NADPH 8 C NR, FAD ). NO 3-, M C [40] NO 2-, NH 3,, F 2^* U8X, 8X2Q RS F ( NR O -., NO - 3 N [41],_ ` a Q Q NO - 3 N [42] MNO_ C3 1, )45mg L -1 `a FA JK,[ NO 3- K N P <, ba NR _ Q, FA NR NO 3- K # ) -\A, bc I T I K, 6 T X A J K [43 46],) M -. A,Q RS ba6 I T JK D, Q RS$& ba I T, bc b I I T ', PQ R S 1/ ) A M 2 H N 8 X,M A P 2 Zn 8X, P P " Q Zn [47] )@A [4] = ( 犑狌犵犾犪狀狊狊犻犵犻犾犪狋犪 ) [9],Q R S _ " N K Fe Zn B 9 H J K K Q )M-.A,)_ `a.45 mg L -1, F / K <,N K Fe Mn Cu B JK, D _ ` a F /G H, Q _ `a H $ / ], W ) ( 犆犪犿犲犾犻犪狅犾犲犻犳犲狉犪 ) [48]

8 T ( 犃犿狔犵犱犪犾狌狊犮狅犿犿狌狀犻狊 ) [49] ( 犣犻狕狔狆犺狌狊犼狌犼狌 犫犪 ) [50] 9 Y F A Ca Mg JK _ ` a <, $ 2 P 2 Ca Mg c N 8X O [2,51], )@ A [4] = [9] 9 WY,_ `a Q Q F /K b G a b, F /G. U F P Q RS P,)Q + F b b G <,b A APaseL b A SAPase, ba I T JK, M P 1 D_ `a F H,) 45 mg L -1 _ +, FA N P K Mn Cu BJK,! Q _ `a FA JK Q8X Q RS FA N J K Q 2 NR O F A P Ca Mg JK _ `a <,Zn JK _ `a < P -.Q R S b / * T Apase I TU, bca I T 2I PQ RS 8X,^ NO! E. A JK"# /G $VLV,C PQ R S T / $ H I _ P 7: [1].-\RS2 H[M]. :" #,1993: [7] :;,.]^ `a /GG bc / [J].A "#,2015,48(3): [2],. H [M]. :, 2003: [3],,.] ^ _ ` a / G L b c [J]. #,2014,31(9): ZHANGD M,SONG X,ZHANG LJ.Efectsofphosphorus fertilizingongrowthandrootmorphologyof 犃犿狅狉狆犺犪犳狉狌狋犻 犮狅狊 a[j]. 犘狉犪狋犪犮狌犾狋狌狉犪犾犛犮犻犲狀犮犲,2014,31(9): [4] :;, B.]^_ `a /GL H J K [J].A " #,2012,45(4): FAN W G,WANGLX.Efectofdiferentphosphoruslevels ongrowthandleafelementcontentsofyoungnewhalnavel orangetrees[j]. 犛犮犻犲狀狋犻犪犃犵狉犻犮狌犾狋狌狉犪犛犻狀犻犮犪,2012,45(4): [5],,,9.Q RS Fbc FAN W G,LUO Y.Growth status,root morphology and physiologicalcharacteristicsoffourcitrusrootstocksunderdif ferentphosphoruslevels[j]. 犛犮犻犲狀狋犻犪犃犵狉犻犮狌犾狋狌狉犪犛犻狀犻犮犪, 2015,48(3): [8],,,9.Q RS Fbc/G [J].A "6,2013,29(34): WU Y,LIP,LIXT, 犲狋犪犾.Efectsoflowphosphorusstresson rootgrowthandacidphosphataseactivityofstrawberryseed lings[j]. 犆犺犻狀犲狊犲犃犵狉犻犮狌犾狋狌狉犪犾犛犮犻犲狀犮犲犅狌犾犲狋犻狀,2013,29 (34): [9],:;,._ `a = /F/G L H JK [J].A "#,2016,45 (4): ZANGCF,FAN W G,PAN XJ.Efectofphosphoruslevels ongrowth,morphologicalcharacteristicsandleafelementcon / [J]. =,2010,27(2): tentsof 犑狌犵犾犪狀狊狊犻犵犻犾犪狋犪 Dode.seedlings[J]. 犛犮犻犲狀狋犻犪犃犵狉犻 犮狌犾狋狌狉犪犛犻狀犻犮犪,2016,45(4): LIYF,JINSH,YEZQ, 犲狋犪犾.Rootmorphologyandphysio logicalcharacteristicsin 犆犪狉狔犪犮犪狋犺犪狔犲狀狊犻狊 seedlingswithlow phosphorusstress[j]. 犑狅狌狉狀犪犾狅犳犣犺犲犼犻犪狀犵犉狅狉犲狊狋狉狔犆狅犾犲犵犲, 2010,27(2): [6],,,9.]^_ `a F b /LM X[J].A / "#,2010,18(4): YUE H,LIG H,CHEN L L, 犲狋犪犾.Clusterrootproduction andputilizationofmacadamiaseedlingunderdiferentptreat ments[j]. 犆犺犻狀犲狊犲犑狅狌狉狀犪犾狅犳犈犮狅 犃犵狉犻犮狌犾狋狌狉犲,2010,18(4): [10],,,9.Q RS F/G 2( Oc[J]. #,2016,52(2): CHENZY,WUPF,ZOU X H, 犲狋犪犾.Relationshipbetween growthandendogenoushormonesofchinesefirseedlingsun derlowphosphorusstress[j]. 犛犮犻犲狀狋犻犪犛犻犾犪狏犲犛犻狀犻犮犪犲,2016, 52(2): [11],,,9. / G 4 ( 犔狌狆犻狀狌狊犪犾犫狌狊 L.)b / [J].A :/,2013,43(3):

9 7,9:_ `a F/G HIJKLMNO/ 1323 TANG H L,SHEN J B,ZHANG F S, 犲狋犪犾.Interactive efectsofphosphorusdeficiencyandexogenousauxinonroot morphologicalandphysiologicaltraitsinwhitelupin ( 犔狌狆犻 狀狌狊犪犾犫狌狊 L.)[J]. 犛犮犻. 犆犺犻狀犪 : 犔犻犳犲犛犮犻.,2013,43(3): [12] LEFEBVERDD,DUFFS M G,FILEC.Responsetophos phatedeprivationinbrassicanigrasuspenioncels[j]. 犘犾犪狀狋犘犺狔狊犻狅犾狅犵狔,1990,93(2): [13], A,,9. 9 a I /TU[J]./ 56,1999,21(3):1 8 LIYJ,LIUJZ,LIB, 犲狋犪犾.Molecularmechanismsofself rescueofhigherplantsforphosphorusstarvation[j]. 犅犻狅 狋犲犮犺狀狅犾狅犵狔犐狀犳狅狉犿犪狋犻狅狀,1999,21(3):1 8. [14] :,,. + bc *[J].A "#,2001,3(2): FAN M S,XU B,WANG Y.Acidphosphataseactivitiesof intactrootsandgroundroottissuesofmaizegrowninhighp orlowpnutrientsolution[j]. 犚犲狏犻犲狑狅犳犆犺犻狀犪犃犵狉犻犮狌犾狋狌狉犪犾犛犮犻犲狀犮犲犪狀犱犜犲犮犺狀狅犾狅犵狔,2001,3(2): [15],,.b / 2 [M]. :A "#<,2009: [16] SHINR,BERGR H,SCHACHTMANDP.Reactiveoxygen speciesandroothairsin 犃狉犪犫犻犱狅狆狊犻狊 rootresponsetonitro gen,phosphorusandpotassiumdeficiency[j]. 犘犾犪狀狋犪狀犱犆犲犾犘犺狔狊犻狅犾狅犵狔,2005,46(8): [17] TEWARIR K,KUMARP,SHARMAPN.Oxidativestress andantioxidantresponsesinyoungleavesofmulberryplants grownundernitrogen,phosphorusorpotassium deficiency [J]. 犑狅狌狉狀犪犾狅犳犐狀狋犲犵狉犪狋犻狏犲犘犾犪狀狋犅犻狅犾狅犵狔,2007,49 (3): [18] LISC,NING T,HUAN XJ, 犲狋犪犾.Changesinorganicacid metabolismdiferbetweenrootsandleavesofcitrusgrandis inresponseto phosphorusandaluminum [J]. 犑狅狌狉狀犪犾狅犳犘犾犪狀狋犘犺狔狊犻狅犾狅犵狔,2009,166(18): [19],,,9. 3/G/ L b QIBL,CAO C L,WANG F, 犲狋犪犾.Influenceoflow phos phorusonnitratereductiveactivityand NO3 N contentin cowpeaseedling[j]. 犃犵狉犻犮狌犾狋狌狉犪犾犚犲狊犲犪狉犮犺犻狀狋犺犲犃狉犻犱犃狉犲 犪狊,2010,28(1): [22] *, <,+,#,9.Q RS 678 F - / `a [J].. / 8,2012,33(11): HEP,WANGDP,WEIJS, 犲狋犪犾.Lowphosphorusstresson thephysiologicalmetabolism of 犺犲狏犲犪 seedling[j]. 犆犺犻狀犲狊犲犑狅狌狉狀犪犾狅犳犜狉狅狆犻犮犪犾犆狉狅狆狊,2012,33(11): [23] 0. H 2 R [M]. :A " #, 1999: [24] :;,!a. RS P[J]. " =,1997,38(3):43 47 FAN W G,LIUJP.Responseofelementsdeficiencystress in 犚狅狊犪狉狅狓犫狌狉犵犺犻 Trat.[J]. 犑狅狌狉狀犪犾狅犳犌狌犻狕犺狅狌犃犵狉犻犮狌犾 狋狌狉犪犾犆狅犾犲犵犲,1997,38(3): [25], 1. H-.[\[M]. :A "#<,2011: [26] :;,!a,,9.]^ /G Z [J].,1998,25(1):27 32 FAN W G,LIU JP,XIANG L, 犲狋犪犾.Efectsofnitrogen formonthegrowthanddevelopmentof 犚狅狊犪狉狅狓犫狌狉犵犺犻 [J]. 犃犮狋犪犎狅狉狋犻犮狌犾狋狌狉犪犲犛犻狀犻犮犪,1998,25(1): [27] BARR S A.Soilnutrientbioavailability:a mechanisticap proach[j]. 犙狌犪狉狋犲狉犾狔犚犲狏犻犲狑狅犳犅犻狅犾狅犵狔,1995,161(2): [28],.PX!U23 4 5[J]. ",1991,6(2): LIN Q M,HUANG D M.Evaluationofanacidphosphatase assayfordetectionofphosphorusdeficiencyintomatoleaves [J]. 犃犮狋犪犃犲犵狉犻犮狌犾狋狌狉犪犲犅狅狉犲犪犾犻犛犻狀犻犮犪,1991,6(2): [29] 6./ O [M]. :A " #, 2000: [30] 7,8. "* I [M]. :A " #,2000: c, [J].,2011,43 (3): JIAZ H,YIJH,FUJG, 犲狋犪犾.Efectsofphosphorustreat mentonflue curedtobaccogrowthphysiologicalcharacteris ticsandrootconfiguration[j]. 犛狅犻犾狊,2011,43 (3): [20]!"#,$%&,'. `a ]^',<4V [J].<4,2007,26(3): CAIBY,GEJP,ZU W.Theafectionofphosphoruslevelto diferentgenotypesoybeannitratereductase(nr)activities [J]. 犛狅狔犫犲犪狀犛犮犻犲狀犮犲,2007,26(3): [21],, (,9. RS 54 F J K [J]. ) " # -.,2010,28 (1): [31] ERICSSON T.Growthandshoot:rootratioofseedlingsin relationtonutrientavailability[j]. 犘犾犪狀狋犛狅犻犾,1995,169(1): [32] 9:. 2 H -. B [M]. : " # <,2001: [33] ;. Q R S -. [J].. / " #,2004,24(2): WANG Y.Strategyofgeneticsresearchonplantstolerantto lowphosphorus[j]. 犆犺犻狀犲狊犲犑狅狌狉狀犪犾狅犳犜狉狅狆犻犮犪犾犃犵狉犻犮狌犾 狋狌狉犲,2004,24(2): [34]. H [M]. : 5,

10 T 1989: [35],, <. -.!=[J]. A"#<,2008,27(1): HUANG Y,ZHANG H W,XU FS.Researchprogresson plantacidphosphatase[j]. 犑狅狌狉狀犪犾狅犳犎狌犪狕犺狅狀犵犃犵狉犻犮狌犾 狋狌狉犪犾犝狀犻狏犲狉狊犻狋狔,2008,27(1): [36] >?,. I b c b c F / G L b c [J]. /,2000,26(2): PANG X,ZHANGFS.EfectsofthepartofP supplyroots oncucumberseedlinggrowth,pconcentrationinshootand rootandsecretedacidphosphataseactivitybyroot[j]. 犘犾犪狀狋犘犺狔狊犻狅犾狅犵狔犑狅狌狉狀犪犾,2000,26(2): [37] AB, C, DE,9. F 01 L Mbc Q RS P[J].,2014,34(5): WENGCF,TANGJ,WULJ, 犲狋犪犾.Sparinglysolublephos phorusabsorptionandrootresponsetophosphorusdeficiency stressin 犈狌犮犪犾狔狆狋狌狊 seedlings[j]. 犃犮狋犪犅狅狋犅狅狉犲犪犾 犗犮犮犻 犱犲狀狋. 犛犻狀.,2014,34(5): [38] DATTA R,SHARMA R.Temporalandspatialregulationof nitratereductaseandnitritereductaseingreeningmaizeleav es[j]. 犘犾犪狀狋犛犮犻犲狀犮犲,1999,144(2):77 83 [39],,. `a `at U -.!=[J].,2004,24(7): LIU L,GAN ZJ,WANG X Z.Advancesofstudiesonthe regulationofnitratemetabolismofplantsatnitratereductase level[j]. 犃犮狋犪犅狅狋犪狀犻犮犪犅狅狉犲犪犾犻 犗犮犮犻犱犲狀狋犪犾犻犪犛犻狀犻犮犪,2004, 24(7): [40] CAMPBELL W H,REDINBAUGH M G.Ferric citratere ductaseactivityofnitratereductaseandit sroleinironas similationbyplants[j]. 犑狅狌狉狀犪犾狅犳犘犾犪狀狋犖狌狋狉犻狋犻狅狀,1984,7 (15): [41] D,:;. /F 2 X I [J].,2017,34(6): WANG M L,FAN W G.Diferencesinabsorptionandutili zationofnitratenitrogenandammoniumnitrogenintheseed lingof 犚狅狊犪狉狅狓犫狌狉犵犺犻 [J]. 犑狅狌狉狀犪犾狅犳犉狉狌犻狋犛犮犻犲狀犮犲,2017, 34(6): [42] D. 2 X [D].>: <,2017. [43] JONESD L,DENNISP G,OWEN A G.Organicacidsbe haviorinsoils misconceptionsandknowledgegaps[j]. 犘犾犪狀狋犛狅犻犾,2003,248(1): [44] DING YZ,LIZA,ZHOUB.Low molecularweightorganic acidsandtheirecologicalfunctions[j]. 犛狅犻犾狊,2005,37 (3): [45] WEILL,CHENCR,XUZH.Citricacidenhancesthemo bilizationoforganicphosphorusinsubtropicalandtropical forestsoils[j]. 犅犻狅犾狅犵狔犪狀犱犉犲狉狋犻犾犻狋狔狅犳犛狅犻犾狊,2010,46(7): [46] WANGS,MULLIGAN C N.Efectsofthreelow molecular weightorganicacids(lmwoas)andphonthemobilization ofarsenicandheavymetals(cu,pb,andzn)from minetail ings[j]. 犈狀狏犻狉狅狀犿犲狀狋犪犾犌犲狅犮犺犲犿犻狊狋狉狔牔犎犲犪犾狋犺,2013,35 (1): [47] F. / [M]. : " # <, 1990: [48] G.`H + / G H I * L H [D]. : #<,2013. [49] I,J, K,9. N P K L H J K [J].A " 6,2014,30(4): YANGB,CHEY H,XU YT, 犲狋犪犾.Theinfluenceofmineral nutrientelementscontentofalmond sleavesonexogenous fertilizerof N,Pand K[J]. 犆犺犻狀犲狊犲犃犵狉犻犮狌犾狋狌狉犪犾犛犮犻犲狀犮犲犅狌犾犲狋犻狀,2014,30(4): [50],,M,9.X B N/G 2 HIO [J]. H 2 R,2012, 18(1): ZHANG T T,XU FL,WANG Y K, 犲狋犪犾.Efectsoffertili zationongrowthandseasonaldynamicofleafnutrientsof closeplantingpear jujubetrees[j]. 犘犾犪狀狋犖狌狋狉犻狋犻狅狀犪狀犱犉犲狉 狋犻犾犻狕犲狉犛犮犻犲狀犮犲,2012,18(1): [51]. V HI L F -.[D]. : A"#<,2008. (:)