33 6 Vol.33No ActaEcologiaeAnimalisDomastici Nov.2012!"#$%,,,,,,! (,! ) [& '] "#$%&' ()*+,-./ ,89:; < & 2 F2G H,

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1 33 6 Vol.33No ActaEcologiaeAnimalisDomastici Nov.2012!"#$%,,,! (! ) [& '] "#$%&' ()*+,-./ ,89:; < & 2 F2G H,&IJKL MNO P 1.QRS TUMV,WX Y "MV, DE+,2GHQ Z [& \U]^,DE+, 1 S _`10~14mm ab,c" ; 1 `10~22mm ; P 1 `7~14mm =>? < 1 MIC MBC 31.25mg/mL, P 1 MIC MBC 62.5mg/mL, P 125mg/mL; < MIC MBC 62.5mg/mL 125mg/mL, P MIC MBC $ 125mg/mL 250 mg/ml;=>? P MIC MBC $ 125 mg/ml 250 mg/ml, MIC MBC 62.5mg/mL 125mg/mL Q Z [&2^,&@ AB 1.88g/kg < =>?3.75g/kg. 1 " 100% DE+,2GH 1 QRS Q Z c TU, TU [ ] +,;1 ;Z MV [ ()] S811.6 [*+,-.] A [*/0)] (2012) "#$%&', ()*+,-. +/ 01 0 [1] 2," ( 56789:; + : <= "#$%&'+; 78,>(?@ ABC D?@+E F;GH, +@ I JKLMNO)* [2] 1994, P Q R / S T U V W + 9 IJ E Z[\E,]^ _`D+ab c56#@ IJ ; c5 6#@IJ + \ c 1 " W 8, ( )@ I ( ) + + :D [3] W + + W ( ( / (@ +D c : / E Z W ( ( 犈狊犮犺犲狉犻犮犺犻犪犮狅犾犻 ). ( ( 犇犻犪狉狉犺犲犪犫犪犮犻犾狌狊 ) ( ( 犛狋犪狆犺狔犾狅犮狅犮犮狌狊犪狌狉犲狌狊 ) (( 犅犪犮犻犾狌狊狊狌犫狋犻犾犻狊 ) ( 8+ ( ( ZG c ( ] I J [4] " W =+ S; 5 : + V!T"#+$% $ & ' 1 ( 78)* ( + (,:",- ( 3W-.8"#1 c/ * 0 1 [ ] , : [ ] ( ); :;: (TY091007) [ ] (1956 ),<,=>:?, 5 :.3 E mial:[email protected]

2 6 : ( * (+ ( 73 1 V 5@ ; 8$&' $% 1 A B!CDE 1 $&' F"GHIJH K LMNO,8 ) P8;$% 6~8 QH #'RLM,RS,T[, P8; UV QH LM,T[, P8; F!GH,LM,T[, P8 W ("WXY ( +W (2 : A B), ( " X Y ( (2 Z: C D) [ ("\] ^ _ [ + \ ` W (2 Z: E F) F abc (NA), X *0, LB b 1 X ; ) b SW CJ 2F 6M b HH 8 ) (Olympus CX41) / W@ ( MV ZX, D "20~22g,!J 3 X MV H F (([2 2W2 )( 50μL, ( 5mLLB b W, /min, b5~6h, +(( +1mL ( ( 5mL LB b W, 37 b24h,05 ( 1.175% 0.5 ml 1% 99.5mL,- (( G+ W), 8 LB > ( B 10 7 cfu/ ml,4 P , 2G $&'50g, 10 Y ) 1.5h, W, " c min,84 S 100mL ),70 30min B50mL, +:1g/mL,pH B7.0~7.2 3 ( 5@ G ( +* ) ( / =70%), 4 24h,1500r/min 5 min S 95% B Y! 80%, 4 24h,1500r/min 5 min S ) " # B [5] ( * $ % & ' (30min,4 P IJ F +@ M + ( W, ~20min (,\ 60 + c" Y, Z ( * W, 40min,\P8 [6] S _ ( * 3 D(, )*+ 4 (37 24h + b 50μL, ( -. A /S a b c b ', 15 min,+ "4 20min,37 b18h \,1Y ( + ( 20mm : 15~20mm : 10~14mm :W 10 mm : [7] S (MIC) ( * W (+ 9 ( 6 ( Z b 2 ml,1 2mL $&'* 22 ml B21, B mL 56, , "1 ~ cfu/ ml( 50μL,5 6 Z b b24h 9:>( ; ( + 9 : 9 ( (MIC) [8] ( G (MBC) ' b 0+ W Z20.1mL b ( abc )*', b 37 b 24h, +( 8 ( 5 <: >( >( +@ 9 :=@ + 9 ((MBC) [8] >, :5?,?6>;? Z@A B10 7 cfu/ml ( C b 0.1 ml 0.2mL 0.3mL 0.4mL 0.5mL, Ub,DE 9:72h, ; 0!90%+ ( 9 Y [10] Q Z [&MV Y:1g/mL ( * Z 8 ) : 0.5g/ ml 0.25g/mL 0.125g/mL g/mL 30

3 74 a V 33 ml,4 P8 260>,< : 26?,? 10 >, Z: 8? 8?,? (4?)? (4?), W,? F ( * + ( GHZG+ Y Z$IJ@(0.3 ml/10g D), 3 DE3d 4dKL B9 Y( ME$IJ@, 3DE3d;??GH B9 Y( 1hF (* + ( GHZG+ Y$IJ@,N, 3DE3d; 8? B9 Y+( 8? B G Y + O ) 7d 8? > [8 9] 2 2.1!"# 8 1 " ( * W ((A B) [ ((E F)W ((C D) ; * W [+ (AW ; $% * W+ (W + ( [+ ( ;$&'* W + (A:W [+ ( ( P $% Q Q Q $&' G +)* ZG+ (R ZG+ ZG+ ( ZG S !45(MIC) ZG * ZG+ (9 ( ZG ( * (+ MIC S 2 2 T, * W 1 [ ( + MIC Z : 62.5 mg/ml 62.5 mg/ml 125 mg/ml; * W 1 [ + ( + MIC:125mg/mL, + 61!78 Table1 Antibacterialefectoftheextractsfrom4speciesofplantsinEscherichiacoliinvitro d/mm Plants A B C D E F 犜犪狉犪狓犪犮狌犿犿狅狀犵狅狀 犆犪狌犾犲狊犔狅狀犻犮犲狉犪犲 $% 犞犲狉犫犲狀犪狅犳犳犻犮犻狀犪犾犻狊 $&' 犘狅狉狋狌犾犪犮犲狅犾犲狉犪犮犲犪 :A B:W (,E F[ ( C D : ( G Note:A Bispig's 犈. 犮狅犾犻,E Fiscow's 犈. 犮狅犾犻,C Dispigeon's 犈. 犮狅犾犻.Thesamebelow !45(MIC) Table2 MICoftheextractsfrom4speciesofplantsonE.coliinvitro mg/ml Plants A B C D E F 犜犪狉犪狓犪犮狌犿犿狅狀犵狅狀 犆犪狌犾犲狊犔狅狀犻犮犲狉犪犲 $% 犞犲狉犫犲狀犪狅犳犳犻犮犻狀犪犾犻狊 $&' 犘狅狉狋狌犾犪犮犲狅犾犲狉犪犮犲犪 mg/ml;$ % * W 1 + ( + MIC : mg/ml [ 62.5 mg/ ml;$&'* W+ MIC :62.5 mg/ml, 1 [ + ( + MIC 125 mg/ml G( +* ZG+ (+ MIC M ZG (MBC) 3 T, * W (+ MBC A : 62.5 mg/ml, [ ( + MBC : 125mg/mL; * W 1 [ (+ MBC : 250 mg/ml, ( + MBC :125mg/mL;$% * W1 (+ MBC :31.25 mg/ml, [ (+ MBC :62.5mg/mL;$&'* W + MIC 125 mg/ml, 1 [ ( + MIC 250mg/mL 1$&'+* + 9 ( 9 (1 7, 1$% 2( * +9 (19 ( G,$% * ( :; G8W B, U, 0! 90% ' + (

4 6 : ( * (+ ( cfu/ml # ZG * ZG+ Y, G+ * ZG+ Y" + ( 8ZG 4 T, ( *?+ ( A0? " ( 2DE8@ 3d, DE8@3d,$% 2( 83(ZG+ Y, + 0!100%, 7.5g/kg13.75g/kg $&'3.75g/kg+ Y, + 0 A! 100% 3 4? ( DE8@3d,$% ~7.5g/ kg+ Y + 3 4, + 0! 100%,F 1.88g/kg+ Y ! 70% 7.5g/kg $&'3.75g/kg+ Y (MBC) Table3 MBCoftheextractsfrom4speciesofplantson 犈. 犮狅犾犻 invitro mg/ml Plants A B C D E F 犜犪狉犪狓犪犮狌犿犿狅狀犵狅狀 犆犪狌犾犲狊犔狅狀犻犮犲狉犪犲 $% 犞犲狉犫犲狀犪狅犳犳犻犮犻狀犪犾犻狊 $&' 犘狅狉狋狌犾犪犮犲狅犾犲狉犪犮犲犪 <= >?@ABC@1 # Table4 Preventionandtherapyexperimentsresultsresultsoftheextractsfrom4speciesofplantsagainst 犈. 犮狅犾犻 Items 犜犪狉犪狓犪犮狌犿犿狅狀犵狅狀 犆犪狌犾犲狊犔狅狀犻犮犲狉犪犲 $% 犞犲狉犫犲狀犪狅犳犳犻犮犻狀犪犾犻狊 $&' 犘狅狉狋狌犾犪犮犲狅犾犲狉犪犮犲犪 8? Controlgroup?Z /(g/ml) Drugconce ntration Y/(g/kg) Dose Y/> Thenum berofmice /> Death number /> Survival number ) 0.3mL/10g D !100%, 7.5g/kg+ Y + 0! 90% $ % $ & ' F 3.75g/kg + Y F7.5g/kg+ Y1 ( A! P0+ W ( 8Z G+ YA 1 (, 0

5 76 a V 33 4X Y\,$&' 3 ( Y"15g/kgH, + 0 W9 Y3.75g/kg 3( D!78 WG8+ ( # / b+,w + ( Z [ b W X b XY ( +\W1 +, [ ( Z[_[XY\]^_[+\` W +,A ]+ ( T, ( ZG+ ( ( ZG, ($% $&'W ; W (A:W ; [ ($% W $&' W (+9 ( $% < 1$&'< ; + $% < 1 <$&'; [+ $% < 1$&' 9 ( 19 (^ E\P0+c 1$% 2( * +9 (1 9 ( G _ $% ( ( ( (+ " `A [10] c56 a (b c + 8 ` c56 Z C / 31 ] a T+ab 1ab+CD + (7= + ( [9] " (2DE8@3d, DE8@3d, P0+ (, + 8 $% 2 ( ~7.5g/kg + Y + 0! 100%, 3.75~7.5g/ kg $&'3.75g/kg+ Y, + 0A! 100% " ( 78 ( * +,$% $&'F3.75g/kg+ Y + 0!100%, 1 F7.5 g/kg+ Y + 0 Z!100%190%,W ( " (A 8 4 Y\,$&' + Y 7.5g/kgH ,X c/ : "#$%&' 8$% $&' 3 4. * 0 c " + 1 ",-b W ZG+ G8Z G+ 3 ( / 3.3!78 E $% $&' 1 ( WA C 3 (3 ),R ( 8 Hernandez [11] V T $ % (1 (A $% W + 3 [9] 78$&') 6( S ( + ( ( 8 [12] 7 8 QH* ( ) 1 ( ) ( + (,2 ( :14.2mm, ( :10.2 mm. [13] 8 Z G R * (1 ( + (, + Q \ 1 + Z G, W 3 ( RC, R 3 3 c(c 3 > a> + > ) * + ab2 a ATP + [14] [15] \,3 a c ^ (ph : 6), (1 ( 8 Katarzyna [16] + M 3 ( + > a ( > H), T>(> DNA 1 RNA + + M 3T$% $&' 1 ( +* (" 13 A P0+ ( 8 ( R + /.8 b Q+ / +4 8 *+: [1]. 8[J].W #$ 2011,47(8): [2].$. ( \ W ( + [J].E #$,2009(4):54 55.

6 6 : ( * (+ ( 77 * ( ([J].W #$$@ 2011(4): [4] U.30( * (1 ( 8 [J]. E V:, 2011,32(5):55 59,65. [5]!"#, _[\]^ ( ( + P [J]. V,2009,25:(3) [6] $. [M].%!: & \ ',2003: 310. [7] # $.12(W@+ ([J]. $ 2010,31(6): [8] W,.$ & ' W ( ( 8 9 : [J]. $ 2010,31(3): [9].$& ' ( [J].W # $ 2010,46(6): [10] (,)**,+,-,.ZGW@ _[\]^ ( ( +9:[J].W P V,2009,25(14): [11] HernandezN E,Tereschuk M L,AbdalaLR.Antimicrobial activityofflavonoidsin medicinalplantsfrom TafidelVale (Tucuman,Argentina)[J].JEthnopharmacol,2000,73(1 2): [12] Q H * + ( [J] ,28(7): [13].) /0,. ZGR * ( 8+ P[J].1 P V,2010,32(1): [14]. 3 ( 8+ [J]. 2004(12): [15]. C ( [J]. /.8,1999(2): [16] Katarzyna Ulanowska,AleksandraTkaczyk,GrazynaKono pa,etal.diferentialantibacterialactivityofgenisteinarising fromglobalinhibitionofdna,rnaandproteinsynthesisin somebacterialstrains[j].archivesofmicrobiology,2006,184 (5): 犛狋狌犱狔狅狀狋犺犲犃狀狋犻犫犪犮狋犲狉犻犪犾犈犳犲犮狋狅犳狋犺犲犈狓狋狉犪犮狋狊犳狉狅犿 4 犛狆犲犮犻犲狊狅犳犘犾犪狀狋狊狅狀犈狊犮犺犲狉犻犮犺犻犪犮狅犾犻 JIN Lan mei,wu Qing lin,ma Yu,ZHOU Ling ling,ao Guo xia,liu Yin yan,zenjiang xue ( 犆狅犾犲犵犲狅犳犃狀犻犿犪犾犛犮犻犲狀犮犲, 犑犻狀犾犻狀犵犐狀狊狋犻狋狌狋犲狅犳犜犲犮犺狀狅犾狅犵狔, 犖犪狀犼犻狀犵, 犑犻犪狀犵狊狌,210038, 犆犺犻狀犪 ) 犃犫狊狋狉犪犮狋 :TopreventthespreadofEscherichiacoliinraisedanimalsandinventnewherbalmedicine, theexperimentadopted 犞犲狉犫犲狀犪狅犳犳犻犮犻狀犪犾犻狊, 犘狅狉狋狌犾犪犮犲狅犾犲狉犪犮犲犪, 犆犪狌犾犲狊犔狅狀犻犮犲狉犪犲 and 犜犪狉犪狓犪犮狌犿犿狅狀犵狅狀 toextractthemedicine.theexperimentuseddilutesolutionofthemedicineonmicetodeterminethebac teria'ssensitivity.itshowedthesensitivityofpig's 犈. 犮狅犾犻 tothedrugismoderateandtherangeofantibac terialdiameterswas10~14mm.thesensitivityofpigeon's 犈. 犮狅犾犻 tothedrugwashighandtherangeof antibacterialdiameterswas10~22mm.thesensitivityofcow's 犈. 犮狅犾犻 tothedrugwashighandtherange ofantibacterialdiameterswas7~14 mm.the 犞犲狉犫犲狀犪狅犳犳犻犮犻狀犪犾犻狊 ' 狊 MICtopig'sandcow's 犈. 犮狅犾犻 were 31.25mg/mL,62.5mg/mL.The 犜犪狉犪狓犪犮狌犿犿狅狀犵狅狀 ' 狊 MICtopig'sandcow's 犈. 犮狅犾犻 were62.5mg/ml, 125mg/mL.The 犜犪狉犪狓犪犮狌犿犿狅狀犵狅狀 ' 狊 MICtopig'sandcow's 犈. 犮狅犾犻 were62.5 mg/ml,125 mg/ml. The 犘狅狉狋狌犾犪犮犲狅犾犲狉犪犮犲犪 ' 狊 MICtopig'sandcow's 犈. 犮狅犾犻 were62.5mg/ml,125mg/ml,whilembcwere 125mg/mL,250mg/mL.The 犆犪狌犾犲狊犔狅狀犻犮犲狉犪犲 ' 狊 MICtopig'sandpigeon's 犈. 犮狅犾犻 were125mg/ml,125 mg/ml,whilembcwere125mg/ml,250mg/ml.theexperimentonmiceshowedwith 犞犲狉犫犲狀犪狅犳犳犻 犮犻狀犪犾犻狊 and 犜犪狉犪狓犪犮狌犿犿狅狀犵狅狀 1.88g/kg, 犘狅狉狋狌犾犪犮犲狅犾犲狉犪犮犲犪 and 犆犪狌犾犲狊犔狅狀犻犮犲狉犪犲 3.75g/kg,thesur vivalrateofmicewas100%.fourplantswerealefectiveto 犈. 犮狅犾犻 and 犞犲狉犫犲狀犪狅犳犳犻犮犻狀犪犾犻狊 hadthebest efect. 犓犲狔狑狅狉犱狊 :plants; 犈狊犮犺犲狉犻犮犺犻犪犮狅犾犻 ;antibacterialtest