,2017,37(8): 犃犮狋犪犅狅狋. 犅狅狉犲犪犾. 犗犮犮犻犱犲狀狋. 犛犻狀. : (2017) 犱狅犻 : /j.issn 犠犚犓犢 "# $%&' & 1,2, 1,2, 1

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1 ,2017,37(8): 犃犅. 犅狉犲. 犗犱犲狀. 犛狀. : (2017) 犱 : /j.issn 犠犚犓犢 "# $%&' & 1,2, 1,2, 1,2, 1,2, 1,2, 1,2,! 1,2 (1 2, ; #$:, ) :; ( 犘狉狌狀狌狊狊狀 Lindli.var 狉犱 J.Y.Zhang 犲.), <=>? CDE cdna,f G H 犠犚犓犢 I J K L M 12 N E cdna O 犘狊犠犚犓犢 7 犘狊犠犚犓犢 14 犘狊犠犚犓犢 20 犘狊犠犚犓犢 21 犘狊犠犚犓犢 22 犘狊犠犚犓犢 29 犘狊犠犚犓犢 31 犘狊犠犚犓犢 32 犘狊犠犚犓犢 33 犘狊犠犚犓犢 40 犘狊犠犚犓犢 46 犘狊犠犚犓犢 75, O bp,ORF O bp,O P N Q K R S T U V W X Y,12 N 犠犚犓犢 I J K L 1.0mmol/L R,:FGM 犘狊犠犚犓犢 Z[V X\]^,Z_ 犠犚犓犢 KL`ab Rc0 M '^ L ()*: ; 犠犚犓犢 KL;STUV PCR +,&-:Q785;Q786./01:A 犐狊狀狀犱犈狓狆狉犲狊狊狀犳犠犚犓犢犎犿犵狌狊犌犲狀犲狊犳狉犿犘狉狌狀狌狊狊狀狏狉. 狉犱 CAIWeiwei 1,2,LIU Tao 1,2,YANG Huali 1,2,XU Yingyan 1,2, XU Yijin 1,2,PAN Dongming 1,2,CHEN Guixin 1,2 (1ColegeofHorticulture,FujianAgricultureandForestryUniversity,Fuzhou350002,China;2InstituteofPostharvestSci enceandtechnologyofhorticultureproducts,fujianagricultureandforestryuniversity,fuzhou350002,china) 犃犫狊狉 :Inthisstudy, 犘狉狌狀狌狊狊狀 Lindli.var. 狉犱 J.Y.Zhang 犲 waschosenasexperiment material,thedilutionpoolpcr methodwasusedtoscreenanormalizedful lengthcdnalibraryof 犘. 狊狀 var. 狉犱 leavesandtoobtain12theful lengthcdnaof 犠犚犓犢 homologousgenes,whichare named 犘狊犠犚犓犢 7, 犘狊犠犚犓犢 14, 犘狊犠犚犓犢 20, 犘狊犠犚犓犢 21, 犘狊犠犚犓犢 22, 犘狊犠犚犓犢 29, 犘狊犠犚犓犢 31, 犘狊犠犚犓犢 32, 犘狊犠犚犓犢 33, 犘狊犠犚犓犢 40, 犘狊犠犚犓犢 46and 犘狊犠犚犓犢 75,respectively.Theirlengthare 1517,1798,2098,1177,1242,1396,2135,1760,2113,1047,1258and700bp,respectively. TheirORFlengthsarerespective11095,1572,1764,1068,1071,1002,1944,1602,1770,963, 1077and672bp.Theyareencodedas364,523,587,355,357,333,647,533,589,320,358and223a minoacids,respectively.theresultsofrealtimepcrshowedthat 犘狊犠犚犓犢 expressionlevelsincreased significantlyaftersprayingwith1.0mmol/lsalicylicacid.thisresultindicatedthat 犘狊犠犚犓犢 mightbe importantregulatoryfactorforinducingplantdefenseresponsebysalicylicacid. 犓犲狔狑狉犱狊 : 犘狉狌狀狌狊狊狀 var. 狉犱 ; 犠犚犓犢 genes;realtimefluorescencequantitativepcr ( 犘狉狌狀狌狊狊狀 Lindli.var. 狉犱 J.Y. Zhang 犲.) (Rosaceae) ( 犘狉狌狀狌狊 ), b 4M M,,, M : ; : : (2013NZ002 1β )!:(1990-),,!"#$%,&' % #$ E mail: @qq.com ()*:+,(,-./,!"%01,&' % #$ E mail: @qq.com

2 [1],,&' : C C, 4V 5, M%4 [2] Rbc04% M ' ( [3], R( a 4% M, a N 4 % F G [4] WRKY L b Rc0 KL M^ L 74 N 犠犚犓犢, M 49N,! R, Z[ "#%X\ $%, Ra c0 M 犃犠犚犓犢 6 犃犠犚犓犢 7 Z [ V &' [5] ; 犃犠犚犓犢 70 R 0M (, ( R 0 M ( [6 9] ; 犃 犠犚犓犢 18b R c 0 Z [ M L K L [9] ) WRKY b R 0 KL M* ^ L [10 11],, 犃犠犚犓犢 48 * ^ Rc0M 犘犚 Z [,+, -. / 0 4 % [12] ; Rc 0 M 犃犠犚犓犢 33 1 犘犚 Z [, _ 犃犠犚犓犢 33 * ^ R c 0 M K L M Z [ [13] ; 犃犠犚犓犢 25 ^ R ( 0, 犃犠犚犓犢 25 Z[, 犘犚 Z [ [14] 犠犚犓犢 KL 135KL+ #%6 [15] 78 犠犚犓犢 KL9 9#$ Z_,: 犞狏犠犚犓犢 25;, 犞狏犠犚犓犢 R,Z[ V X \ ] ^[16] ] # $ Z _, ) WRKY L `a Rc0 KL M( 0 #$ 犠犚犓犢 IJKL<=>?@AB, < = >? A B C D E cdna,fg 犠犚犓犢 IJKLE cdna,+ KL C% ( D O E, 犠犚犓犢 I J K L M% a; <STUV PCR, U 犠犚犓犢 I JKL R MZ[V$%, R c0 KL Z[M 5F, %4] R c0 GH IJ K 1 L@ 1.1 CDE cdna M M bno [2] A8-80 M P DH10B 4 Q2015R3S]T, UVWXY + _, 100 1R%Z[\,] ^,_`ab C,c C R, C; M :B M1R% Z[\12,Q25, 1.0 mmol/l R,O Q h, 4 )1N%, 3N%, 50.5g,<,,] < 犠犚犓犢 "# $23 犇犖犃 %45 = >? 犠犚犓犢 IJKLE cdna M : M 犠犚犓犢 I J K L >? 1), M=> <O [2] M C 犠犚犓犢 "# $23 犇犖犃 %6789 :& ABFGM ] % <, < ncbi(htp:// C < = +; < DNAMAN F R Q K R 9 O E M I J O E; < F ProtParam(htp://web.expasy.org/protparam/) MOE; < ClustalX8.1 F MEGA4.0 C OE 犠犚犓犢 " # $ ; % (1) CD 5 RNA G : R C D 5 RNA < RNA G (Cat # RE 0502T)G (2) C D 5 S T UV PCR : 犠犚犓犢 M <W,>? STUV PCR B(Z1), TaKaRa G H M M PrimeScript TM RT reagent Kit (Cat # RR037A) cdna, = > 20 ) STUV PCR M ; S T U V PCR M KL 犜狌犫狌狀,B<= Nai TubuinF Nai TubuinR(Z1);STUV PCR Light Cycler480ST U V PCR ] C, 犠犚犓犢 M +Z[V <2 - C?, L@ [17] ML@ C 2 W OE 2.1 犠犚犓犢 "# $%&' ; WRKYG1 F WRKYG1 R WRKYG2 F WRKYG2 R WRKYG3 F WRKYG3 R 犠犚犓犢 M/, < <B M13F M13R

3 8, : 犠犚犓犢 IJKLMO Z[OE <=7>? Table1 Primersequencesusedinthisstudy B Primer <= Sequence(5 3 ) B Primer <=(5 3 ) Sequence WRKYG1 F GATGGKTAYAAYTGGAGAAARTAYGG PsWRKY29 qf GCAACCCTCAACCCCACCAATCATTC WRKYG1 R CCDMATCCMAGGAGCTACTACAGRTG PsWRKY29 qr CCCATATTTACGCCACGCCCACAC WRKYG2 F GGMGDAARTAYGGNCAAAAACCNATC PsWRKY31 qf AGCCCAGATCAGCCATCACAAAG WRKYG2 R GAHCCRAANATGBTCATNGTNACNTAC PsWRKY31 qr CGAAACTCTTGCCTTCCTCATGGTA WRKYG3 F TGGAGRAAATATGGVCAAAARGARATTC PsWRKY32 qf GTGTTATCTATGGCTGTTTCAATGTGCT WRKYG3 R TAYMKAGGAAACCACACGTGTARC PsWRKY32 qr AACCAGAACCCAAACGAAGAGTGAAG PsWRKY7 qf CCTGCCTCACAATCCCCATCTGAAA PsWRKY33 qf CCTCTTCTTACTTCGCAATC PsWRKY7 qr GCACAGAACCCTCAGCATCCCC PsWRKY33 qr GTTACTCCATCCCTGTTCTT PsWRKY14 qf ACCAGACACCGCCTTACTAATTCCC PsWRKY40 qf TCTTAATACCAAGCCTCTCCGACTTTTC PsWRKY14 qr TCCTCCACCTCATCACCACCATCTT PsWRKY40 qr GCTCTCACCCATCACCGTCAACAT PsWRKY20 qf GGCATCCCATGATCCAAAAGCAGTTAT PsWRKY46 qf GCCATCATCAAGACTCCCATCTAGC PsWRKY20 qr CAGCAGAGTTGATTCCGACACCAAGA PsWRKY46 qr CACTCTCACTGCTCAAAAGGGATGTT PsWRKY21 qf CCCTTGATTGGCTTCTGTCCATACTTC PsWRKY70 qf AGTCGTCTTGTCCCCTGAGCGG PsWRKY21 qr GGTAATTCCTTCCATTTGATTGGGGCTC PsWRKY70 qr ACTCGGGCTTTTCTCTTCCACCTC PsWRKY22 qf CCCCAACTCACCGCAACTCGC Nai tubuinf TCGGATGATGATGACCTTCTCTGTG PsWRKY22 qr GACCCTCCAAACCCACAAAGAAATC Nai tubuinr CATACACTCGTCTGCGTTCTCCA / D ( 1) < FG12 5 IMKL<=, KL9 +, # :FGKL<= Q WRKY L 12 5K L < = O 犘狊犠犚犓犢 7 犘狊犠犚犓犢 14 犘狊犠犚犓犢 20 犘狊犠犚犓犢 21 犘狊犠犚犓犢 22 犘狊犠犚犓犢 29 犘狊犠犚犓犢 31 犘狊犠犚犓犢 32 犘狊犠犚犓犢 33 犘狊犠犚犓犢 40 犘狊犠犚犓犢 46 犘狊犠犚犓犢 75 犠犚犓犢 I J K L M cd NA E PQKRN O V Z2 2.2 犠犚犓犢 "# $%@ABCD& F G M C D WRKY L WRKY L ) OE, AtWRKY MO, F G M C D WRKY L & ' O 3 (GroupⅠ~Ⅲ, 2 Z3) GroupⅠ 2 N WRKY 8 C 2H 2 W (C X 4 C X H X 1 H ), PsWRKY20 PsWRKY32 PsWRKY33 Q GroupⅠ GroupⅡ WRKY L /N WRKY 8 C 2H 2 W (C X 5 C X 23 H X 1 H), & ' M Q K R < =,GroupⅡ`; O a b c d e PsWRKY40 Q GroupⅡa,PsWRKY31 Q GroupⅡb,PsWRKY75 Q GroupⅡc,PsWRKY21 PsWRKY7 Q GroupⅡd, PsWRKY14 PsWRKY22 PsWRKY29 Q Group Ⅱe PsWRKY46 PsWRKY70 Q GroupⅢ M.DL5000;1. 犘狊犠犚犓犢 7;2. 犘狊犠犚犓犢 20;3. 犘狊犠犚犓犢 22; 4. 犘狊犠犚犓犢 32;5. 犘狊犠犚犓犢 40;6. 犘狊犠犚犓犢 46;7. 犘狊犠犚犓犢 75; 8. 犘狊犠犚犓犢 14;9. 犘狊犠犚犓犢 21;10. 犘狊犠犚犓犢 29; 11. 犘狊犠犚犓犢 31;12. 犘狊犠犚犓犢 33 1 WRKY PCR 4M M.DL5000;1. 犘狊犠犚犓犢 7;2. 犘狊犠犚犓犢 20;3. 犘狊犠犚犓犢 22; 4. 犘狊犠犚犓犢 32;5. 犘狊犠犚犓犢 40;6. 犘狊犠犚犓犢 46;7. 犘狊犠犚犓犢 75; 8. 犘狊犠犚犓犢 14;9. 犘狊犠犚犓犢 21;10. 犘狊犠犚犓犢 29; 11. 犘狊犠犚犓犢 31;12. 犘狊犠犚犓犢 33 Fig.1 Detectionbyagaroseelectrophoresisof WRKYpositiveclones PsWRKY20 AtWRKY20,PsWRKY22 AtWRKY22, PsWRKY29 AtWRKY29,PsWRKY32 AtWRKY32, PsWRKY33 AtWRKY33,PsWRKY40 At WRKY40,PsWRKY46 AtWRKY46,PsWRKY75 AtWRKY75, + ( 2,Z3) 2.3 犠犚犓犢 "# $%E & OG WRKY L 8 M WRKYGQK M DNA W W

4 ! １４９６ " ;２ # $ % & ３７ ê 犠犚犓犢 lm6n Ｔｂ ２ ＷＲＫＹ ｎｓ ｏｎ ｏ ｓ 狀ｖ 狉犱 ｐ uv Ｇｎ ＤＮＡ ij Ｆｕ ｎｇ ｈｄｎａ ｂｐ ʱËÌÍ ＯＲＦ ｂｐ u Ａｍ ｎｏ ｄ y ＭＷ ｋｄ Ýñò 狆犐 stuv Ｈｏｍｏ ｏｇｏｕｓｇｎ Vö { ｏｎｓ ｖｄｍｏ 犘狊犠犚犓犢７ １５１７ １０９５ ３６４ ３９ ６６ ９ ３２ 犃犠犚犓犢７ 犘狊犠犚犓犢１４ １７９８ １５７２ ５２３ ５ ５５ ０７ 犃犠犚犓犢１４ 犘狊犠犚犓犢１５ １０２０ ９０６ ３０１ ３３ ７３ ９ ９３ 犃犠犚犓犢１５ 犘狊犠犚犓犢２０ ２０９８ １７６４ ５８７ ６４ １２ ００ 犃犠犚犓犢２０ ｘ２ 犘狊犠犚犓犢２１ １１７７ １０６８ ３５５ ４０ ０６ ９ ６１ 犃犠犚犓犢２１ 犘狊犠犚犓犢２２ １２４２ １０７１ ３５７ ３９ ２８ ８３ 犃犠犚犓犢２２ 犘狊犠犚犓犢２９ １３９６ １００２ ３３３ ３７ ６４ ２８ 犃犠犚犓犢２９ 犘狊犠犚犓犢３１ ２１３５ １９４４ ６４７ ７０ ３２ ３９ 犃犠犚犓犢３１ 犘狊犠犚犓犢３２ １７６０ １６０２ ５３３ ５８ ８１ ５４ 犃犠犚犓犢３２ ｘ２ 犘狊犠犚犓犢３３ ２１１３ １７７０ ５８９ ６４ ５９ ７４ 犃犠犚犓犢３３ ｘ２ 犘狊犠犚犓犢４０ １０４７ ９６３ ３２０ ３ ３９ ８ ７２ 犃犠犚犓犢４０ 犘狊犠犚犓犢４６ １２５８ １０７７ ３５８ ３９ ７６ １１ 犃犠犚犓犢４６ 犘狊犠犚犓犢７０ １１８１ １０１１ ３３６ ３７ ２５ ９６ 犃犠犚犓犢７０ 犘狊犠犚犓犢７５ ７００ ６７２ ２２３ ２ ４９ ８ ９７ 犃犠犚犓犢７５ %«ví ì２ 犠犚犓 stuv.'() ＷＲＫＹ %&v w ø;m= 犘狊犠犚犓犢 ｈｏｍｏ ｏｇｏｕｓｇｎ ｓ ｂｏｘｄ Ｆ ２ Ｐｈｙ ｏｇｎ ｏ ＷＲＫＹ ｎｓ ｏｎ ｏ ｓ 狀ｖ 狉犱 ｎｄ犃狉犫 犱狆狊 狊 ｇ ｐ

5 ８$ 011 Ý 犠犚犓犢 stuvwy!. yi ;３ 犠犚犓犢 lm6n78e Ｔｂ ３ ｓ ｓ ｏｎｏ ＷＲＫＹ ｎｓ ｏｎ ｐ ｏ ｓ 狀ｖ 狉犱 uv Ｇｎ Wz Ｇ ｏｕｐ { Þ& Ｄｏｍ ｎｐ ｎ ùú { Ｚ ｎ ｎｇ 犘狊犠犚犓犢７ Ⅱｄ Ｘ５ ２Ｈ２ 犘狊犠犚犓犢１４ １４９７ W * w ＷＲＫＹ V ö ì ３ Ｇ ｏｕｐⅠ ＰｓＷＲＫＹ２０ ＰｓＷＲＫＹ３２ } ＰｓＷＲＫＹ３３ w Ｎ ' } '( １ x w ＤＮＡ Ú Ｎ ' ２Ｈ２ ù ú { Ｘ４ Ｘ２２ Ｈ Ｘ１ Ｈ ＰｓＷＲＫＹ３２ } ＰｓＷＲＫＹ３３ w ' ２Ｈ２ ùú { Ｘ４ Ｘ２３ Ｈ Ｘ１ Ｈ ) ＰｓＷＲＫＹ２０ w ' Ⅱ Ｘ５ ２Ｈ２ 犘狊犠犚犓犢１５ Ⅱｄ Ｘ５ ２Ｈ２ 犘狊犠犚犓犢２０ Ⅰ Ｘ４ ２Ｈ２ １ x * ù ú { Ｘ４ Ｙ Ｘ２２ Ｈ Ｘ１ Ｈ Ｇ ｏｕｐ Ⅱ ＰｓＷＲＫＹ７ ＰｓＷＲＫＹ１４ ＰｓＷＲＫＹ２１ 犘狊犠犚犓犢２１ Ⅱｄ Ｘ５ ２Ｈ２ ＰｓＷＲＫＹ２２ ＰｓＷＲＫＹ２９ ＰｓＷＲＫＹ３１ ＰｓＷＲＫＹ４０ 犘狊犠犚犓犢２２ Ⅱ Ｘ５ ２Ｈ２ 犘狊犠犚犓犢２９ Ⅱ Ｘ５ ２Ｈ２ } ＰｓＷＲＫＹ７５ １ x w ＤＮＡ Ú １ x ２Ｈ２ ù ú { Ｘ５ Ｘ２３ Ｈ Ｘ１ 犘狊犠犚犓犢３１ Ⅱｂ Ｘ５ ２Ｈ２ 犘狊犠犚犓犢３２ Ⅰ Ｘ４ ２Ｈ２ 犘狊犠犚犓犢３３ Ⅰ Ｘ４ Ｘ２３ Ｈ Ｘ１ Ｈ ２Ｈ２ 犘狊犠犚犓犢４０ Ⅱ Ｘ５ ２Ｈ２ 犘狊犠犚犓犢４６ Ⅲ Ｘ４ Ｘ２３ Ｈ Ｘ１ ２Ｈ 犘狊犠犚犓犢７０ Ⅲ Ｘ４ Ｘ２３ Ｈ Ｘ１ ２Ｈ 犠犚犓犢 45,67;<8= Ｌ ù s Ç+ gh １ ０ ｍｍｏ Ⅱ Ｘ５ ２Ｈ２ þ 犠犚犓犢 uv w67 < ì ４ 犘狊犠犚犓犢７５ ì３ Ｈ Ｇ ｏｕｐⅢ ＰｓＷＲＫＹ４６ １ x w Ú ２Ｈ ù ú { ＤＮＡ Ｘ７ Ｘ２３ Ｈ Ｘ１ Ｈ ì ３ ２ ４ ＷＲＫＹ Vö { ÑÑ`aÇC Ｆ ３ Ａ ｏ ｏｎｓ ｖｄｍｏ ｓｏ ＷＲＫＹ 狀ｖ 狉犱 ｇ ｇｎｍｎ

6 R(1.0mmol/L) 12N 犠犚犓犢 IJKL MZ[ Fig.4 Relativequantitiesof 犠犚犓犢 homologousgenesin 犘. 狊狀 var. 狉犱 underthetreatmentof 1.0mmol/Lsalicylicacid 犠犚犓犢 K L 犘狊犠犚犓犢 20 犘狊犠犚犓犢 32 犘狊犠犚犓犢 33 犘狊犠犚犓犢 7 犘狊犠犚犓犢 21 犘狊犠犚犓犢 29 犘狊犠犚犓犢 31 犘狊犠犚犓犢 46 R M Z [ I, ], 30h Z [ V [, ; 犘狊犠犚犓犢 22 ], R 12h Z [ V [ ; 犘狊犠犚犓犢 40 R,6!, 1h Z[V"[H, #$]^, +Z[V, 30h +Z[V ; 犘狊犠犚犓犢 14Z[ ]^ ^ ]^M, 30hZ[V[ 3 % I 3.1 犠犚犓犢 $ %E &- WRKY &' W M V W M & ' O 3, O M WRKY 1N8 W Q Group Ⅱ,Group Ⅲ + [18],WRKY Z 'M ^ L, U ( + ^ KL ' (OM WRKY L U ( <=(NLS)U Q 0 OFGM125 WRKY L M WRKY W 7) WRKYGQK *O8, 0U ( <=,+ WRKY L 犠犚犓犢 F#GHIJKL% MN WRKY L b R c 0 K L M^ L 6 #$Z_, 49N 犠犚犓犢 MZ[, R M ^ [5] 犠犚犓犢 KL 13 5KL+ R # %6 [15] 7 8 WRKY K L 9 9 # $ Z _,: 犞狏犠犚犓犢 25;, 犞狏犠犚犓犢 R,Z[V X \ ] ^[16] ] # $ Z _, ) WRKY L `a R L-, STUV PCR,+ WRKY L R M +Z[V COE,#$ F GM WRKY L R M, R a c 0 犠犚犓犢 7 犠犚犓犢 33 犠犚犓犢 40 犠犚犓犢 46 犠犚犓犢 75 Z [ V M & '. # $ M 犘狊犠犚犓犢 33 犘狊犠犚犓犢 7 犘狊犠犚犓犢 40 犘狊犠犚犓犢 46 R c 0 Z [ V & ' M W, WRKY7b, Z [ 犠犚犓犢 7 / H KLMZ[,0 犘犚 1 [19] ; 犃犠犚犓犢 33, Rc 0Z[V & ', b 狑狉犽狔 33 1 M#$,Z _ 犃犠犚犓犢 33 R ( R M 2,+ R 0M 3* ^ M ) <, ( R [20], 7 8 犞狏犠犚犓犢 33 # $,4 犞狏犠犚犓犢 33 # R 0M,& [21] 犅狀犠犚犓犢 33 Z [,a 7 8 R (R 0M, 9 : M ;, % U M [22],WRKY40 ),* ^ K L ^ L M Z [,0 犈犇犛 1 犘犃犇 4 [23] " 犠犚犓犢 40, RM5 c0 [24] 犠犚犓犢 46 Z [`&5 + M,A+#$Z_ 犠犚犓犢 46 R( [25] Z[ 犠犚犓犢 75 ` / P M %,A+< # $ Z _ 犃犠犚犓犢 75 ^ R 06 P ( [26] ] # $ Z_ ) WRKY L `a R 0 KLM #$W Z_, R I 8c0H WRKY L MZ[, b= R 0 ( >?#$

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