Microsoft Word 沈颂东_news_.doc

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1 条斑紫菜分子生物学研究现状 Molecular biology research status of Porphyra yezoensis 沈颂东 (, ) 中图分类号 : S96 文献标识码 : A 文章编号 : (2014) doi: /hykx (Porphyra yezoensis Ueda) (Rhodophyphyta), (Protoflorideophyceae), (Bangiales), (Bangiaceae), (Porphyra), 130 [1] 22 [2],,, 20, 2/3, ( 3g), 32, 1 条斑紫菜生活史 (P. yezoensis),,,,,,, , A 4 B 4 C 8, A 2 B 4 C 8, 16, A 2 B 2 C 4,,, n=3( 1) [2] 1 2 紫菜的分子生物学研究 1993 [3] DNA ;,, Stiller [3] : ; : : ( ) : (1968-),,,, :, shensongdong@suda.edu.cn Marine Sciences / Vol. 38, No. 5 /

2 P. rediviva rrna (small subunit rdna, SSU rdna) B/E- (internal transcribed spacer, ITS) RFLP, P. purpurea,,, Niwa [4] RFLP 1,, SSU rdna ITS PCR-RFLP Niwa, DNA He [5] AFLP(amplified fragment length polymorphism), 40 88,,,, 98.6%, 80.7%; 0.61, 0.71 : Shivji [6] 20 90, Reith [7] P. purpurea, 46, rbcl ITS, DNA, DNA Yang [8] Solexa ; [9] (P. yezoensis) Solexa, 220 Mbp, GC 53.08%; , , 2.22 ; bp, 319 bp; 2011 Shen [10] (SSH),, ( ) ( ), EST(Expressed sequence tag), 360 NCBI 181 EST HS HS572762, 179 EST HS HS EST BLAST, 196, 92, 104 GO (58%); DNA /, ;, DNA GO ; He [11] MicroR- NAs, 14 mirnas, mirnas, RNA,, [12],, Wang [13] 6 Wu [14] 7, 122 / 2014 / 38 / 5

3 , GAPDH [15] alternative oxidase (AOX), 1650 bp, ORF 1332 bp, 443 qpcr, AOX RNA (rdna),,, DNA 10% rrna, 18S rdna(small subunit, SSU) 5.8S rdna 28S rdna(large subunit, LSU),, (internal transcribed spacer, ITS), (intergenic spacer, IGS) rdna ( 2), ITS1 ITS2 18S 5.8S 28S 18S rdna (ETS), ITS ETS rdna, IGS, IGS, [16] 2 18S~28SrDNA rrna,,,, 18S rdna 28S rdna 3,, 18S 28S, 5.8S 160, 18S rdna 28S rdna rdna,, [17-18] 18S rdna,, 800~10000, 18S rdna DNA, 18S rdna,, 18S rdna, PCR, [19-20], 28S rdna, PCR, 28S rdna [21-22] Broom [23] 49 18S rrna,,,, Kunimoto [24] 9 18S rdna, SSU rdna,,,, rrna 4, 18S DNA(rDNA) (ITS) (IGS),, IGS rdna, IGS, IGS ITS,, [25] ITS IGS Mizukami [26] ITS, ITS, ITS Niwa [27] 7 ITS1 rdna,, Marine Sciences / Vol. 38, No. 5 /

4 7 ITS1, 7 Kunimoto [28] SSU rdna ITS, SSU rdna,,, ITS1, ITS, IGS ITS, ITS, ITS [18] IGS, ITS, IGS ITS, IGS, IGS 2009 IGS, 1100 bp, 25, Journal of Applied Phycology [29] 28S rrna, IGS 2011, Sutherland [30] 15 Journal of Phycology, 18S rdna rbcl,, 15, 8 Porphyra, Pyropia, Wildemania 5 22 Pyropia,,, 28S rdna IGS : [1] Yoshida T.Catalogus of species of Porphyra in the world, with special reference to the type locality and bibliography[j]. Nat Hist Res Special Issue, 1997, 3: 5-8. [2],,,. [M]., : 2005: [3] Stiller J W, Waaland J R. Molecular analysis reveals cryptic diversity in Porphyra (Rhodophyta)[J]. Fish Sci, 1993, 62: [4] Niwa K, Kikuchi N, Aruga Y. Morphological and molecular analysis of the endangered species Porphyratenera (Bangiales, Rhodophyta)[J].J Phycol, 2005, 41: [5] HeL, Zhu J, Lu Q, et al. Genetic similarity analysis within Pyropia yezoensis blades developed from both conchospores and blade archeospores using AFLP[J]. J Phycol, 2013, 49: [6] Shivji M S. Organization of the chloroplast genome in the red alga Porphyra yezoensis[j]. Cur Gene, 1991, 19: [7] Reith M, Munholland J. A high resolution gene ma p of the chloroplast genome of the red alga Porphyra purpurea[j]. Plant Cell, 1993, 5: [8] Yang H, Mao Y X, Kong F N, et al. Profiling of the transcriptome of Porphyra yezoensis with Solexa sequencing technology[j]. Chinese Sci Bull, 2011, 56: [9],,,. [J]., 2011, 35(6): [10] Shen S D, Zhang G C, Li Y Y, et al.comparison of RNA expression profiles on generations of Porphyra yezoensis (Rhodophyta), based on suppression subtractive hybridization (SSH)[J]. BMC Research Notes, 2011, 4: 428. [11] He L, Huang A, Shen S, et al. Comparative Analysis of micrornas between sporophyte and gametophyte of Porphyra yezoensis[j]. Comp and Func Genomics, 2012, 9(1): [12] Wang J F, Jiang P, Cui Y, et al. Gene transfer into conchospores of Porphyra haitanensis (Bangiales, Rhodophyta) by glass bead agitation[j]. Phycol, 2010, 49: [13] Wang G L, Zhao G, Feng Y B, et al. Cloning and comparative studies of seaweed trehalose-6-phosphate synthase genes[j].mar Drugs, 2010, 8: [14] Wu X, Huang A, Xu M, et al. Variation of expression levels of seven housekeeping genes at different 124 / 2014 / 38 / 5

5 life-history stages in Porphyra yezoensis[j]. PLoS ONE, 2013, 8(4): [15] Zhang B Y, He L, Jia Z, et al. Characterization of the alternative oxidase gene in Porphyra yezoensis (Rhodophyta) and cyanide-resistant respiration analysis[j]. J Phycol, 2012, 48(3): [16] Leskinen E, Pamilo P. Evolution of the ITS sequences of ribosomal DNA in Enteromorpha (Chlorophyceae)[J].Hereditas, 1997, 126: [17] Blomster J, Hoey E M, Maggs CA, et al. Species-specific oligonucleotide probes for macroalgae: molecular discrimination of two marine fouling species of Enteromorpha (Ulvophyceae)[J]. MolEcol, 2000, 9: [18] Sallares R, Brown T A. Phylogenetic analysis of complete 59 external transcribed spacers of the 18S ribosomal RNA genes of diploid Aegilops and related species (Triticeae, Poaceae)[J]. Gene Res and Crop Evol, 2004, 51: [19] Muller K M, Sheath R G, Vis M L, et al. Biogeography and systematics of Bangia (Bangiales, Rhodophyta) based on the rubisco spacer, rbcl gene and 18S rrna gene sequences and morphological analyses in North America[J]. Phycologia, 1998, 37(3): [20] Sandra V T, Lindstrom C, Saunders G W. Phylogeny of the Dumontiaceae (Gigartinales, Rhodophyta) and associated families based on SSU rdna and internal transcribed spacer sequence data[j]. J Phycol, 2001, 37: [21] Lellaert F, Rousseau F, Reviers B D, et al. Phylogeny of the Cladophorophyceae (Chlorophyta) inferred from partial LSU rrna gene sequences: is the recognition of a separate order Siphonocladales justified?[j].eur J Phycol, 2003, 38: [22],,. 28S rdna [J]., 2007, 31(4): [23] Broom J E, Nelson W A, Yarish C, et al. A reassessment of the taxomomic status of Porphyra suborbiculata, Porphyra carolinensis and Porphyra lilliputiana(bangiales, Rhodophyta) based on molecular and morphological data[j]. Eur J Phycol, 2002, 37: [24] Kunimoto M, Kito H, Cheney D P, et al. Discrimination of Porphyra species based on small subunit ribosomal RNA gene sequence[j]. J Appl Phycol, 1999, 11: [25],,,. rdna IGS [J]., 2008, 34(9): [26] Mizukami Y, Kito H, Kaminishi Y, et al. Nucleotide sequence variation in the ribosomal internal transcribed spacer regions of cultivated (cultivars) and field collected thalli of Porphyra yezoensis[j]. Fish Sci, 1999, 65: [27] Niwa K, Aruga Y. Rapid DNA extraction from conchocelis and ITS-1 rdna sequences of seven strains of cultivated Porphyra yezoensis (Bangiales, Rhodophyta)[J]. J Appl Phycol, 2003, 15: [28] Kunimoto M, Kito H, Mizukami Y, et al. Molecular features of a defined genetic marker for determination of the Porphyra tenera lineage[j]. J Appl Phycol, 2003, 15: [29] Li Y Y, Shen S D, He L H, et al.sequence analysis of rdnaintergenic spacer (IGS) of Porphyra haitanensis[j] J Appl Phycol, 2010, 22: [30] Sutherland J E, Lindstrom S C, Nelson W A, et al.a new look at an ancient order: Generic revision of Bangiales (Rhodophyta)[J]. J Phycol, 2011, 47, ( 本文编辑 : 梁德海 ) Marine Sciences / Vol. 38, No. 5 /

Microsoft Word - 11-黄惠珍_new_.doc

Microsoft Word - 11-黄惠珍_new_.doc 36 6 Vol.36, No.6 2012 6 JOURNAL OF FISHERIES OF CHINA June, 2012 文章编号 : 1000-0615(2012)06-0884-09 DOI: 10.3724/SP.J.1231.2012.27758 坛紫菜色素突变体色素基因的表达定量分析,,,, * (, 361021) : 6, PCR 4 (Cpeα Cpcα Apcβ Chl.a)