33 2 2014 4 CHINESE JOURNAL OF MARINE DRUGS Vol.33 No.2 April2014 * * ( 266003) : 从罗非鱼皮中提取了明胶 并以驴皮中提取的阿胶为阳性对照 在对比研究两者理化性质的基础上 研究了两者对缺铁性贫血的改善效果 采用饲喂低铁饲料辅助尾静脉放血的方法制备大鼠缺铁 性贫血模型 灌胃不同剂量的阿胶和罗非鱼皮明胶 罗非鱼明胶和阿胶的紫外最大吸收峰都在 232nm 左右 ;2 种明胶的氨基酸组成相似 均符合胶原蛋白 ( 明胶 ) 的氨基酸组成特点 ; 罗非鱼皮明胶中的 K Mg 含量 显著高于阿胶 而阿胶中的 Cu Fe Na Cd Cr 含量明显高于罗非鱼皮明胶 ; 罗非鱼皮明胶能够促进大鼠体重 的恢复 提高血红蛋白 (Hb) 含量 红细胞数 (RBC) 等外周血指标 显著促进大鼠贫血症状的改善效果 罗非鱼皮明胶与阿胶都具有较好的抗贫血活性 : 明胶 ; 缺铁性贫血 ; 罗非鱼皮 ; 驴皮 ; 理化性质 :R965 :A :1002-3461(2014)02-045-06 DOI:10.13400/j.cnki.cjmd.2014.02.008 Studyonthephysicochemicalpropertiesandantianemiaactivity ofgelatinfromtilapiaskin YANG XiaHOU HuGUO MingCHANG Yu-feiBU LinNIU Hui-naLIBa-fang * (Colegeof Food Scienceand EngineeringOcean Universityof ChinaQingdao266003China) Abstract:ObjectiveTostudytheefectonimprovingIrondeficiencyanemia(IDA)ofgelatinfromtila- piaskinthegelatinfromtilapiaskinanddonkeyskinwasextracted.usingthedonkeyskingelatinas positivecontrolthephysicochemicalpropertieswerecompared.methodstheida modelratswerees- tablishedbybloodletingviatailveinandfeeding withiron-deprivedfoodassisted.diferentdosesof twokindsofgelatinweregiventothemodelratsviaintragastricadministration.resultstheultraviolet absorptionmaximumpeakoftilapiaskingelatinanddonkeyskingelatinwerebothatabout232nm;the twokindsofgelatinhadsimilaraminoacidcompositionbothconformingtothecolagen(gelatin)ami- noacidcompositioncharacteristics.theresultsofmineralanalysisshowedthatthereweremorekand MginthetilapiathanthoseinthedonkeyskingelatinwhilethecontentsofCuFeNaCdandCrof donkeyskingelatinwerenotablyhigherthanthoseofthetilapiaskingelatin.itwasevidentthattheti- lapiaskingelatincouldsignificantlypromoteweightrecoveryofidaratsaswelasthecontentofhb andrbc.itturnedouttilapiaskingelatincouldevidentlyimprovethesymptomsofanemiaofida. ConclusionBoththegelatinfromtilapiaskinanddonkeyskinexhibitedthepromisinganemiaactivity. Keywords:gelatin;Irondeficiencyanemia(IDA);tilapiaskin;donkeyskin;physicochemicalproperty * : (201205027); (20100508); (201313002); : (1988-) * : Tel.:0532-82031852 E-mail:bfli@ouc.edu.cn :2013-10-08
46 33 (1 6 )40 min ; 20.1% 0.2mol/L 20 min(1 4 ) ; 75 5h6000r/min 10min 2.2 阿胶的提取 1 10 5 d 2 121 10 min 121 30 min(1 6 ) 121 2 70% 2.3 低铁饲料的配制 AIN-93M ICP-AES 4.58mg/kg( 135.23mg/kg) 2.4 明胶的紫外光谱分析 0.5 mol/l 1 2.5 明胶的氨基酸组成分析 SPF 4 Wistar 70~90g 5mL Hitachi835-50 2.6 矿物质成分分析 ; ICP- (HH-4 ) AES ; (Alpha1-4LD ) Christ 2.7 动物造模方法 ; (LDZX-50KBS ) ; (Hitachi835-50 ) ; 1mL 1 7 (ICP-AESHK-2000 ) (Hb) (BC3000) 100g/L ; (1100 ) 2.8 动物分组与实验 2 2.1 罗非鱼皮明胶的提取 5cm 6% 0.5g/L UV-2550 200~400nm 100nm/min 0.5nm 6 mol/l 110 24h [1] NaCl 10h(1 10 ) 30d 20μL 0.2% NaOH 50μL [2-3] 4 1 6 7 100mg kg -1 500 mg kg -1
2 : 47 ) 2.9 大鼠体重测量 3d 2.10 外周血象指标 20μL 3 (Hb) (RBC) (PLT) (MCV)) 2.11 网织红细胞计数 [4] C=O n π* 10g/L 50μL 50μL 283nm 251nm 2 283nm 5 min 252nm 2 1000 2.12 血清铁测定 ( 30 min 3.1 明胶的紫外光谱分析 1 233nm 231nm Fig.1 1 (a: ;b: ) Ultravioletabsorptionspectroscopyofgelatin (a:donkeyskin;b:tilapiaskin) 3.2 明胶的氨基酸组成分析 1 2 19.97% [6] 2 ( + ) 3.3 明胶的矿物成分分析 : 25.54% 2 2 10 20.65% K Mg Ca Mn [5] Zn Cu Fe Na Cd Cr
48 33 1 Table1 Aminoacidcompositionofgelatinfromdonkeyskinandtilapiaskin (Aminoacidresidues/100totalaminoacidresidues) Aminoacidcomposition Donkey-hidegelatin Tilapiaskingelatin ASP 5.71 5.49 THR 2.40 2.62 SER 4.70 5.16 GLU 10.34 10.67 GLY 19.97 23.09 ALA 7.93 10.96 CYS / / VAL 2.98 2.26 MET 0.84 1.42 ILE 1.61 0.98 LEU 3.23 2.54 TYR 1.06 0.51 PHE 1.85 1.98 LYS 3.02 3.4 HYP 9.32 7.4 HIS 0.97 0.75 ARG 7.83 8.50 TRP / / PRO 16.22 12.25 2 Table2 Mineralsofgelatinfromdonkeyskinandtilapiaskin /ng g -1 Ca Cd Cr Cu Fe K Mg Mn Na Zn Donkey-hidegelatin Tilapiaskingelatin 10198.42 3.36 73.06 107.1 642.15 2060.66 119.34 19.48 14829.6 239.88 10414.62 0.97 50.72 94.61 507.31 2780.28 815.82 19.67 11591.4 231.67 3.4 大鼠缺铁性贫血 (IDA) 模型的建立 3 (Hb) (RBC) (HCT) (MCV) (PLT) (Hb) 3 100g/L Hb RBC HCT MCV PLT 3 (MCV) Table3 ComparisonoftheindicatorsofcontrolgroupandIDA modelgroup Hb/g L -1 RBC(10 12 /L) HCT/% MCV(fl) PLT 154.5±10.95 8.17±0.48 41.67±2.07 48.97±1.3 669.5±22.4 Controlgroup Modelgroup 91.03±6.74* * 4.84±0.87 * * 18.78±2.85 * * 40.43±0.69 * 447.3±22.1 **
2 : 49 3.5 明胶对 IDA 大鼠体重的影响 (Hb) 2 (RBC) MCV (PLT) 4 MCH (GDL) (P<0.05)Hb RBC MCV PLT (P<0.05) (P<0.01) (GTH) (GDH) GDL GDH GTH (P<0.01) Hb (P<0.01) 420g 2 GTL Hb (P <0.05) GDH RBC MCV MCH (P<0.01) IDA GDH RBC PLT (P<0.01)MCV MCH (P<0.05)GHL RBC MCV (P< 0.05) IDA 2 IDA Fig.2 Efectofgelatinfromdonkeyskinandtilapiaskin onbodyweight(bw)ofida modelrats (500mg/kg) 3.6 IDA 大鼠外周血象分析 (100mg/kg) 4 4 IDA Table4 Efectofgelatinfromdonkeyskinandtilapiaskinonthechangeof peripheralhemogramtargetsofida modelrats Targets Hb RBC MCV MCH PLT Controlgroup 156.5±12.21 7.78±0.65 48.96±1.28 23.23±1.63 658.5±19.4 Modelgroup 102.17±6.74 # # 5.64±0.87 # # 40.43±0.68 # # 19.86±1.27 # 477.3±22.1 # # GDL 123.67±5.82 * * 6.16±0.44 * 40.75±0.97 21.01±1.34 471.2±37.2 GDH 134.33±9.16 * * 7.27±0.26 * * 45.31±1.92 * * 22.60±1.47 * 689±42.8 * * GTL 117.50±2.81 * 6.90±0.35 * 43.58±1.32 * 20.92±0.46 482±32.4 GTH 138.00±2.61 * * 7.45±0.32 * * 43.13±1.73 * 21.77±0.70 * 605.6±47.9 * * 3.7 网织红细胞计数 IDA : 33.8±5.55 36.9±8.81
50 33 GDL35.5±9.08 GDH34.1±5.52GTL36.2±8.71GTH34.1± 8.89 3.8 血清铁含量测定 [9] IDA 3 IDA SI (P<0.01) GDL GDH [1] 3 IDA [6]. [J]. 2009122(4):79-84. Fig.3 Efectofgelatinfromdonkeyskinand tilapiaskinonthechangeofsiofida modelrats 4 [9] (1):43-46. [7] [8] 2 (23.09%) 2. [D]. : 2009. [2].ICP AES.[J] 201131(4):1119-1121. [3].ICP-AES [J]. 200623(4):723-726. [4]. [J]. 201233(2):260-261. [5] LiHLiuBLGaoLZetal.Studiesonbulfrogskincol- lagen[j].foodchemistry200484(1):65-69. [7].. [J]. 19891(6):27. [8]. [J]. 199924(1):54-56.. [J]. 1991