海葵化学成分及生物活性研究进展 Review of sea anemones compound and bioactivity 史文军 1, 秦松 3, 张朝晖 1,2 2, 吴建平 (1., 212001; 2., 226007; 3., 264003) 中图分类号 : R931.6 文献标识码 : A 文章编号 : 1000-3096(2013)12-0122-10 (sea anemone), (Coelenterata) (Anthozoa) (actiniaria) 6 37 [1],,,, : (Anthopleura xanthogrammica) (A.pacifica) (Haliplanella luciae Hand) (Cereus sinensis Verrill), 20 70 [2-3],,, Ap-A Ap-B, G- [4], ; [5], PsTX ; Tejuca [6] 3, 3,, [7], [8] 1 蛋白肽类成分与生物活性 ( ),,, [3] 1.1 神经毒类成分与生物活性, [2,9-10] Norton [11] 3, 1 2 3 1 2 46~49, 6 3 ; 1 ApA ApB ATX II ; 2 RT I RT II 3 27~32,, 8 4 ; 3 DaI DaII ATX III 1 3, [12], 3, 1 2 3 1 35~37, 6 3, 1 (Kv) [13] ; 1 Stichodactyla helianthus : 2012-12-25; : 2013-03-12 : ( AS20120006) : (1987-),,,, :, E-mail: muzhiye080326@126.com;,, : 13951791950, E-mail: zzh6495@eyou.com 122 / 2013 / 37 / 12
ShK [14] Bunodosoma granulifera BgK [15] Anemonia erythraea AETX K [16] 2 3, 2 AsKC-1 AsKC-2 AsKC-3 [17] ; 3 Anemonia sulcata BDS-I BDS-II [18] Anthopleura elegantissima APETx1 [19] APETx2 [20] APHC1 [21],,, [22], α -,, [22-24] Cline E I [25] Urticina piscivora UpI, [26] Ap-QD1 Ap-QD2, 2 AP-A AP-B, Ap-QD1 Ap-QD2 [27] AP-Q, AP-Q I to I K I K 1, AP-Q rhk2a,,, [28] rhk2a N-, rhk2a,,, Stichodactyla helianthus ShK Kv1.3, Kv1.3 T B, ShK [29],,, : BcIV [30] AETX-II AETX-III [31], Am-I Am-II [32] BcIV Joacir Stolarz Oliveira B.caissarum, Callinectes danae, 2 000 μg/kg Tomohiro Honma [33] Stichodactyla haddoni 4, SHTX I-IV, SHTX I-III, SHTXIV ; Tomohiro Honma [34] Dofleinia armata Entacmaea ramsay 2 3, 3, Kazuo Shiomi [35-36] Hiroshi Nagai [37] 1.2 溶细胞毒类成分与生物活性,,, 4 1 5~8 kda,, ; 2 20 kda,,, ; 3 30~40 kda, ; 4 80 kda,, [38-39], [40] Gary R. Grotendorst David A. Hessinger [41] Aiptasia pallida,, Yawen Wang [42] HMgIII, Wang, 1.. 20, 37 20 min, 30%~100% Bragadeeswaran Subramanian [7] 4,, P.indicus H.magnifica P.sinensis ; S.haddoni Batista [43], EqtII 0.1 nmol/l V-79-379A,,, Jiang [44] SrcI BGC-823 BEL-7402 NSCIC, Abel Santamaria [45] Maria Cristina Pico [46] Elena V. Klyshko [47] Joacir Marine Sciences / Vol. 37, No. 12 / 2013 123
Stolarz de Oliveira [48] 1.3 其他蛋白多肽类成分与生物活性 [49] (Src), Src (VCMC),, Src, 100 mg/l (VSMC) Src [50] Ant-5,, Kazuo Shiomi [36] gigantoxin I, 31%~33%, A431 gigantoxin I A431, Ricardo B. Cunha [51] CGX,,,, 8~12 h, CGX,, : Armando Alexei Rodriguez [52] Stichodactyla helianthus Bunodosoma granulifera, cdna, 5 APETx 5 1 Armando A.Rodriguez [53] ph Phymanthus crucifer, MALDI-TOF-MS, 2.0~3.5 kd Andre Junqueira Zaharenko [54] Bunodosoma cangicum, Sephadex G-50, 3, 3 1 : CGTX-II -AITX-Bcg1a -AITX- Bcg1b, 3, [55],, 1 48 AX-1, AX-1 - -,, Steve Peigneur [56] Anthopleura elegantissima, APEKTx1, 1 63 2, Kunitz APEKTx1 Kv1.1, 1 Mikiko Maeda [57] Cryptodendrum adhaesivum Heterodactyla hemprichii Thalassianthus aster 3, 3 ;, 1 -TLTX-Ca1a 2 - TLTX-Hh1a -TLTX-Hh1c 1 -TLTX- Ta1a,, 2 Andrej Razpotnik [58] Urticina crassicornis, Sephadex G-50, UcI, 30 kd 1, N-,,,,, 124 / 2013 / 37 / 12
表 1 部分蛋白质 多肽类毒素氨基酸序列 Ap-A GVSCLCDSDGPSVRGNTLSGTLWLYPSGCPSGWHNCKAHGPTIGWCCCKQ Ap-B GVPCLCDSDGPRPRGNTLSGILWFYPSGCPSGWHNCKAHGPNIGWCCKK SHK RSCIDTIPKSRCIAICCKHSMKYRSICRKICGC BgK VCRDWFKETACRHAKSLCNCRTSCKYRANCAKTCELC AsKC-1 INKDCLLPMDVGRCRASHPRYYYNSSSKRCEKFIYGGCRGNANNFHTLEECEVCGVR AsKC-2 INKDCLLPMDVGRCRARHPRYYYNSSSRRCEKFIYGGCRGNANNFITKKECEKVCGVR AsKC-3 INGDCELPKVVGRCRARFPRYYYNLSSRRCEKFIYGGCGGNANNFHTLEECEKVCGVRS BDS-I AAPCFCSGKPGRGDLWILRGTCPGGYGYISNCYKWPNICCYPH BDS-II AAPCFCPGKPDRGDLWILRGTCPGGYGYTSNCYKWPNICCYPH APETx1 GTTCYCGKTIGIYWFGTKTCPSNRGYTGSCGYFLGICCYPVD BcIV GLPCDCCHGHTGTYWLNYYSKCPKGYGYTGRCRYLVGSCCYK APETx2 GTACSCGNSKGIYWFYRPSCPTDRGYTGSCRYFLGTCCTPAD Am-II ALLSCRCEGKTEYGDKWLFHGGCPNNYGYNYKCFMKPGAVCCYPQN CGTX-II GVACRCDSDGPTVRGDSLSGTLWLTGGCPSGWHNCRGSGPFIGYCCKK δ-aitx-bcg1a GVPCLCDSDGPSVRGNTLSGTVWVFGCPSGWHICTSDGPTIGSCCKK δ-aitx-bcg1b GVPCLCDSDGPSVRGDTLSGTVWVFGCPSGWHICTSDGPTIGSCCKK AX-1 GGVPCLCDSDGPSVRGNTLSGIIWLAGCPSGWHNCKAHGPTIGWCCKQ APEKTx1 INSICLLPKKQGFCRARFPRFYYNSSTRRCEMFYYGGCGGGNANNFNTLEECEKVCLGYGEA WKAP δ-tltx-ca1a VACKCDDDGPDVRSATFTGTVDLGSCNSGWEKCASYYTVIADCCRKPRG δ-tltx-ta1a VACKCDDDGPDIRSATLTGTVDLGSCDEGWEKCASYYTVIADCCRRPRS,,,, 1 2 3 pi 8.0, 4 pi 5.0,, [39],, 2 酰胺类成分与生物活性 [59-61], Anthcpleura pacifica,, 7 ( 1) : 2S, 3R, 4 (E), 8 (E)- - (1) 2S, 3R, 4 (E), 8 (E)- - (2) 4,5 (E), 8,9 (E)- - (3) N- -N- -(2S, 3R)- -4(E), 8(E)- (4) N- -N-(9Z- )-(2S, 3R)- -4(E), 8(E)- (5) N- - N- -(2S, 3R)- -4(E), 8(E )- (6) N- -N-(13Z- )-(2S, 3R)- - 4(E), 8(E)- (7) 2S, 3R, 4 (E), 8 (E)- - 2S, 3R, 4 (E), 8 (E)- - 4,5 (E), 8,9 (E)- - N- -N- -(2S, 3R)- - 4(E), 8(E )- [62] 4,5 (E), 8,9 (E)- - 3 甾体类成分与生物活性 [60] Anthcpleura pacifica 5α, 8 - -6, 22- -3 - (8), 2 4 糖和糖苷类成分与生物活性 [63] Marine Sciences / Vol. 37, No. 12 / 2013 125
1 [65] Anthopleura midori [66] Anthopleura xanthogrammica 1- - - (9), 3 2 Zoanthus stephensoni, 2, 2 2, I, ; II,, [64] 3 5 脂肪酸和甘油酯类成分与生物活性 [67] Anthopleura xanthogrammica 5 126 / 2013 / 37 / 12
( 4), 2 : 9,12- (10) 9,14- (11); 3 :1-o- -2-o-(9- )-3-o-(9,12- ) (12) 1-o- -3-o-(14- ) (13) 1-o-(9,12- )-2-o-(9,12- ) (14) [59] Anthcpleura pacifica : (15) (16) 1-o- (17) [62] SD, (8 mg/kg), ; (33 mg/kg), 4 6 生物碱类成分与生物活性 [68] HPLC Haliplanella luciae Hand, 3 ( 5): tryptoline(18) 1- - - (19) - (20); 1 : 2- -3- -6- -9 - (21), 5 Marine Sciences / Vol. 37, No. 12 / 2013 127
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