2013 35 2 307-312 http / /xuebao. jxau. edu. cn Acta Agriculturae Universitatis Jiangxiensis E - mail ndxb7775@ sina. com SSR SCAR 310024 RAPD EST - SSR SCAR 26 24 22 18 81. 8% 7 23 S668. 4 Q943. 2 A 1000-2286 2013 02-0307 - 06 Establishment of DNA Fingerprinting and Analysis of Genetic Relationship among Main Strawberry Fragaria ananassa Cultivars in Southern China XIN Ya RUAN Song-lin MA Hua-sheng WANG Shu-zhen FANG Xian-ping XIAO Wen-fei WU Gen-liang Hangzhou Academy of Agricultural Sciences Hangzhou 310024 China Abstract RAPD EST - SSR and SCAR markers were used to establish DNA fingerprinting and to analyze the genetic diversity among 26 main strawberry cultivars in Southern China. 22 primer pairs showed amplification among 24 published primer pairs of which 18 primer pairs which accounted for 81. 8% showed polymorphism. 7 pairs of primers were selected as the core markers and used to establish DNA fingerprinting among 23 strawberry cultivars which could distinguish them clearly. Moreover the clustering analysis and the dendrogram of main strawberry cultivars in southern China were investigated according to the amplification pattern. The results provide effective technology support for the identification of chaotic varieties of main strawberry cultivars in southern China. Key words strawberry cultivars fingerprinting molecular markers relationship Fragaria ananassa Duch. 2012-11 - 13 2012-12 - 17 2008BADA6B06 1979 E - mail xinya0@ yeah. net
308 35 1 20 90 DNA 2 RAPD 3-5 AFLP 6 GISH RAPD 7 RAPD SCAR 32 8 RAPD SSR 9-10 1 1. 1 17 1 03-6 - 2 14 9-80 DNA CTAB 11 1. 2 1. 2. 1 引物合成 8 RAPD 8 6 SCAR 8 10 EST - SSR 9 R1 ~ 8 S1 ~ 6 E1 ~ 10 1. 2. 2 PCR 扩增和产物检测 PCR MG96 + PCR 8-9 RAPD SCAR DNA marker DL2000 EST - SSR GeneRuler 100 bp 15 g /L 8. 0% Bio - Rad Gel Doc XR Bio - Rad GS - 800 1. 3 1 0 NTSYS - pc UPGMA 2 2. 1 24 Tenira DNA PCR 26 DNA 24 S4 S522 Tenira DNA Fig. 1 1 Gento 2 Tenira 3 Rimona 4 Aroma 5 Bella - rosse 6 Elvira 7 Kletter 8 stugarta9 Ferma 21 22 23 24 1 Gento 2 Tenira 3 Rimona 4 Aroma 5 Bella - rosse 6 Elvira 7 Kletter 8 stugarta9 Ferma 21 Fengxiang 22 Qianxi 23 Liyinǚ 24 Shimeisihao. 1 RAPD 13 Amplification products obtained from isolates using marker R6 in 13 cultivars.
2 SSR SCAR 309 91. 7% R1 R2 R5 E1 18 81. 8% 1 2 3 RAPD R6 SCAR S1 S3 EST - SSR E2 E3 E4 E7 E8 1 Gento 2 Tenira 3 Rimona 4 Aroma 5 Bella - rosse 6 Elvira 7 Kletter 8 stugarta9 Ferma 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 03-6 - 2 26 1 Gento 2 Tenira 3 Rimona 4 Aroma 5 Bella - rosse 6 Elvira 7 Kletter 8 stugarta9 Ferma 10 Zuoheqingxiang 11 Baojiaozaosheng 12 Tongziyihao 13 Dasai 14 Falandi 15 Fujieniya 16 Huaji 17 Hongshimei 18 Guinugan 19 Zhangji 20 Hongjia 21 Fengxiang 22 Qianxi 23 Liyinǚ 24 Shimeisihao 25 03-6 - 2 26 Haiyu. M DNA marker DL2000 M DNA marker DL2000 Fig. 2 2 SCAR 26 Amplification products obtained from isolates using SCAR markers in 26 cultivars. 1 Gento 2 Tenira 3 Rimona 4 Aroma 5 Bella - rosse 6 Elvira 7 Kletter 8 stugarta9 Ferma 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 03-6 -2 26 1 Gento 2 Tenira 3 Rimona 4 Aroma 5 Bella - rosse 6 Elvira 7 Kletter 8 stugarta9 Ferma 10 Zuoheqingxiang 11 Baojiaozaosheng 12 Tongziyihao 13 Dasai 14 Falandi 15 Fujieniya 16 Huaji 17 Hongshimei 18 Guinugan 19 Zhangji 20 Hongjia 21 Fengxiang 22 Qianxi 23 Liyinǚ 24 Shimeisihao 25 03-6 -2 26 Haiyu. M DNA marker DL2000 M DNA marker DL2000 Fig. 3 3 EST - SSR 26 Amplification products obtained from isolates using EST - SSR markers in 26 cultivars.
310 35 2. 2 RAPD 7 26 A - G 4 11 5 7 4 2 2 4 M DNA marker DL2000 1-11 M DNA marker DL2000 1-11 Nunber of Polymorphic band types. Fig. 4 4 7 26 Polymorphic band types obtained from isolates using 7 core markers in 26 cultivars 26 1 1 7 Gento Tenira Rimona aroma bellarosse Kletter Ferma 03-6 -2 21 03-6 -2 1 Elvira Stugarta 7 Tab. 1 1 26 Name origin and fingerprinting code of 26 strawberry Germplasm DNA Code Cultivars Origin DNA fingerprinting Code Cultivars DNA Origin DNA fingerprinting 1 Gento A2B3C1D1E2F1G1 14 Falandi A2B8C1D6E4F1G2 2 Tenira A1B1C2D2E2F1G2 15 ** Fujieniya A2B2C2D6E2F1G2 3 Rimona A3B3C1D3E1F2G2 16 Huaji 4 Aroma A1B2C3D2E2F1G2 17 Hongshimei 5 Bella - rosse A3B3C2D3E1F2G2 18 ** Guinugan 6 Elvira * A3B4C2D4E2F1G1 19 Zhangji 7 Kletter A2B5C4D3E2F1G2 20 Hongjia 8 stugarta * A3B4C2D4E2F1G1 21 Fengxiang 9 Ferma A3B6C5D5E2F2G2 22 Qianxi A2B9C1D6E2F1G2 A2B9C1D6E4F1G2 A2B2C2D6E2F1G2 A2B2C1D6E2F1G2 A2B9C2D6E2F1G2 A4B10C1D6E4F1G1 A4B7C2D6E3F1G2 10 A4B7C1D6E3F1G2 23 Liyinǚ Zuoheqingxiang A4B9C1D6E4F1G2 11 12 Baojiaozaosheng A2B2C1D6E4F1G1 24 A2B11C1D4E4F1G1 Shimeisihao A1B7C1D6E3F1G2 25 03-6 - 2 A2B6C1D7E2F1G2 Tongziyihao 13 ** Dasai A2B2C2D6E2F1G2 26 Haiyu A4B10C1D4E4F1G2 * ** * and ** indicate the varieties that can t distinguish each other. 2. 3 1 0 Excel NTSYS - pc
2 SSR SCAR 311 UPGMA 5 5 Fig. 5 26 Dendrogram of 26 strawberry cultivars 5 26 0. 63 4 Gento 3 8 a- roma bellarosse 0. 32 Elvira Stugarta 1 0. 58 03-6 - 2 0. 59 1 0. 79 0. 86 0. 96 0. 82 0. 96 Elvira Stugarta 0. 86 1 Gento 03-6 - 2 0. 86 0. 68 3 DNA 14 7 26
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