2012 13 2 201-206 Journal of Plant Genetic Resources / 071001 41 SSR 21 SSR 41 52 2 ~ 4 2. 5 Shannon 0. 21 ~ 1. 40 0. 73 5. 54 3 7 9 13. 16% ~ 186. 49% 5. 48 6 11 SSR Genetic Diversity of Peanut Arachis hypogaea L. Varieties Bred in Hebei Shandong and Henan Provinces GENG Jian LIU Li-feng CUI Shun-li CHEN Huan-ying WEI Xin-yan Key Laboratory of Crop Germplasm Resources of Hebei Province / College of Agronomy Agricultural University of Hebei Baoding 071001 Abstract In order to assess the genetic diversity 41 peanut varieties bred in Hebei Henan and Shandong provinces were used with methods of SSR markers phenotypic identification and cluster analysis. Using 21 pairs of SSR primers 41 peanut varieties were screened and 52 alleles were detected with 2-4 alleles per locus and average of 2. 5. Shannon information index of varieties ranged from 0. 21 to 1. 40 with an average was 0. 73 for each pair of primers. Cluster analysis based on SSR markers showed that 3 groups and 7 subclasses were clustered while threshold was 5. 54. The coefficient of variation for nine kinds of agronomic traits were at range of 13. 16% - 186. 49%. And the result of cluster analysis based on agronomic traits showed that these varieties were divided into 6 categories and 11 subclasses when threshold was 5. 48. Varieties in each category have their own characters. Key words Peanut Varieties SSR Genetic diversity Arachis hypogaea L. 48% 56% 1 2 3 24 SSR 16 0. 09 ~ 0. 82 2011-01-02 2011-12-08 10960120D C2009000591 E-mail xtreegj@ hotmail. com E-mail liulifeng@ hebau. edu. cn
202 12 0. 59 4 AFLP 15 1 42. 03% 1. 1 DNA 5 RAPD 41 1 12 1. 2 1. 2. 1 DNA 0. 1g 73. 33% 0. 1128 ~ 0. 3665 ph 6 10 DNA 9 40μl TE SSR STS 20ng /L 7 1. 2. 2 SSR SSR 167 8 75 15μl PCR 40ng DNA 1 PCR Buffer 0. 5U Taq 4mM dntp 41 95 5min 95 30s SSR 1min 72 1. 5min 35 72 10min 5% 80W 40min Sanguinetti 10 1 Table 1 The peanut varieties Code Name Code Name 1 9742 15 5 29 11 2 9814 16 1 30 14 3 9818 17 2 31 2000 4 2 18 8258 32 9 5 4 19 9515 33 9819 6 5 20 21 34 18 7 6 21 22 35 20 8 8 22 23 36 22 9 4 23 25 37 23 10 08-4211 24 9519 38 37 11 9806 25 1 39 08 12 10 26 58 40 1 13 1 27 9633 41 8 14 4 28 9847 Code Name Shannon 11 I = - P i lnp i 1 0 P i i SSR DPS Version7. 05 12 / 100%
2 203 1. 2. 3 2009 2010 2. 1 1. 5m 0. 4m 41 9 2 3 9 13 20% 5 2 Table 2 Performance and variance of agronomical characters 2 % Trait Max Min Mean s V R CV cm Stem length 69. 46 39. 48 54. 09 7. 12 50. 71 29. 98 13. 16 Total of branch 8. 40 4. 80 6. 18 0. 85 0. 71 3. 60 13. 67 g Biomass / Plant No. of three pods 91. 02 34. 17 56. 33 14. 10 198. 81 56. 85 25. 03 2. 20 0. 00 0. 22 0. 42 0. 18 2. 20 186. 49 Total of pods 40. 80 14. 47 23. 46 6. 50 42. 28 26. 33 27. 72 g Pod mass /Plant Hollow pod number / Plant g 100-pod weight g 100-seed weight 3. 00 0. 63 1. 47 0. 64 0. 41 2. 37 43. 40 63. 68 18. 94 33. 01 10. 38 107. 66 44. 74 31. 43 239. 74 123. 26 188. 92 29. 65 879. 40 116. 48 15. 70 98. 46 49. 62 77. 15 12. 38 153. 29 48. 84 16. 05 19. 12% Ⅱ 1 1 5. 48 41 6 Ⅰ 8% Ⅲ 8 2 6 39. 53% Ⅱ 18 3 Ⅳ 4 1 6 5 2. 2 / 5 Ⅲ 6 2 888. 46% Ⅳ 4 1 Ⅴ Ⅴ 3 9742 2 9 Ⅵ 5 14. 56% 27. 88% 38. 06% 48. 81% Ⅵ 1 3 50. 22% 22% Ⅰ
204 12 Fig. 1 1 Dendrogram of cluster analysis based on agronomic trait 2. 2 SSR 167 15 21 41 AFLP 52 2 3 12. 57% 2 ~ 4 2. 5 16 17 AFLP Fig. 2 2 PMc468 41 SSR The pattern of 41 cultivars amplified by PMc468 2. 3 SSR SSR 21 SSR Shannon Shannon 0. 21 ~ 1. 40 0. 73 3 SSR 4 Shannon 0. 7 2. 4 SSR SSR 3 3 41 5. 54 3 I 3 9 4 4 II 10 2 3 III 9 4 3 2 II I 3 3. 1 14 RAPD
2 205 3 Table 3 SSR 41 Shannon SSR SSR Markers Numbers of alleles and Shannon information index for each SSR marker No. of alleles Shannon I Shannon information index PM42 4 4 4 4 1. 31 1. 19 1. 20 1. 39 PM93 1 1 1 1 0. 30 0. 30 0. 37 0. 33 PM137 2 2 2 2 0. 54 0. 46 0. 50 0. 48 PMc468 3 3 3 3 0. 98 0. 95 0. 82 0. 90 PMc660 3 3 3 3 0. 60 0. 96 1. 05 0. 88 PM188 2 2 2 2 0. 50 0. 50 0. 56 0. 49 PM106 1 1 1 1 0. 10 0. 33 0. 25 0. 21 PM145 1 1 1 1 0. 37 0. 33 0. 31 0. 34 PM346 3 3 2 3 0. 71 0. 92 0. 63 0. 73 PM179 3 3 3 3 0. 92 1. 01 1. 00 1. 00 S112 2 2 2 2 0. 65 0. 67 0. 73 0. 71 PM36 4 4 4 4 1. 33 1. 32 1. 47 1. 40 PM50 2 2 2 2 0. 71 0. 47 0. 65 0. 63 PM375 2 2 2 2 0. 39 0. 61 0. 60 0. 50 PM3 3 3 2 3 1. 01 0. 87 0. 69 0. 96 PM377 2 2 2 2 0. 60 0. 61 0. 61 0. 59 PM343 3 3 3 3 1. 07 0. 97 0. 79 0. 95 PM305 2 2 2 2 0. 56 0. 56 0. 51 0. 54 PM325 3 3 3 3 0. 91 0. 96 1. 04 0. 98 PM296 2 3 3 3 0. 67 0. 80 0. 52 0. 60 PM15 3 3 3 3 0. 97 0. 80 0. 91 0. 91 4 Table 4 Genetic diversity of varieties bred in different provinces 3. 2 Shannon I No. Shannon information index No. of alleles Hebei 18 0. 71 51 Henan 10 0. 74 52 Shandong 13 0. 74 50 Total 41 0. 73 52 DNA Shannon Nei' 18 19 20 21 22 Jiang 23 Tang 24 167 SSR 21 8 Shannon Nei'
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