872314B002064M14

: : ( 1/3 ) : NSC 87-2314-B002-064-M14 : 86. 8. 1 87. 7. 31 : :

(OSF) T OSF OSF T T4 T8 B - 58 OSF ( 50 8 ) OSF OSF 45.2 64.2 T B 8 OSF CD4/CD8 2.17 / T T 30 OSF, OSF OSF TGF-β1, TGF-β3 OSF TGF-β2, arecoline,, OSF, arecoline,, TGF-β1 PDGF, arecoline,, OSF TGF-β1 OSF TGF-β1 OSF TGF-β1, arecoline,, OSF PDGF

(OSF) T OSF OSF T T4 T8 B - 58 OSF ( 50 8 ) OSF OSF, OSF OSF EIA OSF IL-1β, IL-6, arecoline,, OSF

1 ( ) ( ) OSF OSF 50 8 10 10 10 2-1 Anti- B IgG 1/200 Parrafin DAKO CD20 1/400 Cryostat Anti- T IgG 1/50 Parrafin Bio CD43 1/100 Cryostat Genex Anti- IgG 1/50 Parrafin DAKO CD68 1/100 Cryostat 2-2 Anti-CD4 T4 IgG 1/10 Cryostat DAKO Anti-CD8 T8 IgG 1/50 Cryostat DAKO 3 (mm) (mm) (mm) 50 0.01 0.15 0.03 50 0.08 0.51 0.27 50 0.50 3.98 1.50 4-1 Mean SD Mean SD Mean SD Mean SD T cell 48.3 24.3 97.7 51.4 190.2 52.4 271.2 107 B cell 0.1 0.1 0.1 0.2 11.2 14.0 15.3 18.4 Macrophage 0.1 0.2 0.1 0.2 13.3 14.2 17.3 19.1 Mean S.D.(standard deviation) ESL(epithelial surface length) /mm ESA(epithelial surface area) /mm 2 CTL(connective tissue length) /mm CTA(connective tissue area) /mm 2

4-2 Mean SD Mean SD Mean SD Mean SD T 96.8 64.8 402.4 302.6 604.4 412.9 859.1 422.9 B 0.6 1.85 1.9 4.6 51.3 41.8 72.6 51.0 M 2.1 5.9 7.8 14.9 45.2 43.6 64.2 53.2 4-3 Mean SD Mean SD Mean SD Mean SD T 47.0 38.1 234.0 199.0 261.9 102.6 316.5 171.4 B 0.2 0.5 1.1 2.5 41.9 37.3 56.2 37.7 M 0.8 1.1 5.6 9.2 43.4 55.6 69.1 101.9 4-4 Mean SD Mean SD Mean SD Mean SD T 19.6 11 60 38.8 255.5 155.5 294.6 81.7 B 0.4 1.3 1.4 3.5 46.0 39.2 63.4 44.3 M 1.4 4.0 6.6 11.9 45.3 50.2 66.9 83.3 4-5 Mean SD Mean SD Mean SD Mean SD T 201.8 133.5 332.8 224.3 1080.2 877.7 1785.5 1271.7 B 0.7 1.0 1.3 1.8 139 204.1 196.6 267.2 M 12.4 21.4 24.0 39.5 141.8 164.2 222.4 217.3 5 T CTL CTA CD4(+) CD8(+) CD4(+) CD8(+) CD4/CD8 1 58.6 32.3 58.6 28.7 2.04 2 129.6 54.0 112.7 48.4 2.33 3 215.2 146.6 191.3 106.6 1.79 4-1 157.8 80.2 450.9 229.3 1.97 4-2 137.8 49.7 152.6 61.1 2.49 5 216.4 100.9 216.4 89.7 2.41 6 100.5 57.1 401.7 194.1 2.07 7 33.0 15.5 122.0 54.3 2.25 131.1 67.0 213.3 101.5 2.17+0.22

6-1 Mean SD Mean SD Mean SD Mean SD N 0.1 0.2 0.1 0.2 13.3 14.2 17.3 19.1 H 0.8 1.5 2.4 4.5 33 22.8 34.3 22.6 SM 2.1 5.9 7.8 14.9 45.2 43.6 64.2 53.2 SA 0.8 1.1 5.6 9.2 45.3 55.6 69.1 101.9 D 12.4 21.4 24.0 39.5 141.8 164.2 222.4 217.3 SM moderate stage of OSF SA advanced stage of OSF N(normal mucosa) H(hyperkeratosis) S(submucous fibrosis) D(dysplasia) 6-2 T Mean SD Mean SD Mean SD Mean SD N 48.3 24.3 97.7 51.4 190.2 52.4 271.2 107.0 H 19.6 11.0 60.0 38.8 255.5 155.5 294.6 81.7 S M 96.8 64.8 402.4 302.6 604.4 412.9 859.1 422.9 S A 47.0 38.1 234.0 199.0 261.9 102.6 316.5 171.4 D 201.8 133.5 332.8 224.3 1080.2 877.7 1785.5 1271.7 6-3 B Mean SD Mean SD Mean SD Mean SD N 0.1 0.1 0.1 0.2 11.2 14 13.3 18.4 H 0.1 0.1 0.1 0.3 11.4 10.9 13.7 15.6 S M 0.6 1.85 1.9 4.6 51.3 41.8 72.6 51.0 S A 0.8 1.1 5.6 9.2 43.4 55.6 69.1 101.9 D 0.7 1.0 1.3 1.8 139 204.1 196.6 267.2 7-1 N - P < 0.05 NS NS S P < 0.05 - NS NS H NS NS - NS D NS NS NS - NS not significant

7-2 N - P < 0.05 NS NS S P < 0.05 - NS NS H NS NS - NS D NS NS NS - 7-3 N - P < 0.05 P < 0.05 P < 0.05 S P < 0.05 - NS NS H P < 0.05 NS - NS D P < 0.05 NS NS - 7-4 N - P < 0.05 NS P < 0.05 S P < 0.05 - P < 0.05 NS H NS P < 0.05 - P < 0.05 D P < 0.05 NS P < 0.05-8-1 T N - NS P < 0.05 P < 0.05 S NS - P < 0.05 P < 0.05 H P < 0.05 P < 0.05 - P < 0.05 D P < 0.05 P < 0.05 P < 0.05-8-2 T N - P < 0.05 NS P < 0.05 S P < 0.05 - P < 0.05 NS H NS P < 0.05 - P < 0.05 D P < 0.05 NS P < 0.05-8-3 T N - P < 0.05 NS P < 0.05 S P < 0.05 - NS P < 0.05 H N S P < 0.05 - P < 0.05 D P < 0.05 P < 0.05 P < 0.05 -

8-4 T N - P < 0.05 NS P < 0.05 S P < 0.05 - NS P < 0.05 H NS P < 0.05 - P < 0.05 D P < 0.05 P < 0.05 P < 0.05-9-1 B N - NS NS NS S NS - NS NS H NS NS - NS D NS NS NS - 9-2 B N - P<0.05 NS NS S P<0.05 - P<0.05 NS H NS P<0.05 - NS D NS NS NS - 9-3 B N - P<0.05 NS NS S P<0.05 - P<0.05 NS H NS P<0.05 - NS D NS NS NS - 9-4 B N - P<0.05 NS NS S P<0.05 - P<0.05 NS H NS P<0.05 - NS D NS NS NS - 10-1 ( ) E S L E S A C T L C T A SM SM SM SM N P < 0.05 P < 0.05 P < 0.05 P < 0.05 H NS NS NS P < 0.05 D NS NS NS P < 0.05

10-2 ( ) E S L E S A C T L C T A SA SA SA SA N P < 0.05 P < 0.05 P < 0.05 P < 0.05 H NS NS NS NS D NS NS NS NS 11-1 ( ) T E S L E S A C T L C T A SM SM SM SM N P < 0.05 P < 0.05 P < 0.05 P < 0.05 H P < 0.05 P < 0.05 P < 0.05 P < 0.05 D P < 0.05 NS NS P < 0.05 11-2 ( ) T E S L E S A C T L C T A SA SA SA SA N NS P < 0.05 NS NS H P < 0.05 P < 0.05 NS NS D P < 0.05 NS P < 0.05 P < 0.05 12-1 ( ) B E S L E S A C T L C T A SM SM SM SM N NS NS P < 0.05 P < 0.05 H NS NS P < 0.05 P < 0.05 D NS NS NS NS 12-2 ( ) B E S L E S A C T L C T A SA SA SA SA N NS P < 0.05 P < 0.05 P < 0.05 H NS NS P < 0.05 P < 0.05 D NS NS NS NS

13 24, TGF-β1 TGF-β1 (pg/ml) Control 167.2 + 1.2 5 µg/ml arecoline 166.4 + 7.5 15 µg/ml arecoline 179.5 + 2.5 30 µg/ml arecoline 147.3 + 7.2 50 µg/ml 133.0 + 3.4 100 µg/ml 106.3 + 3.1 150 µg/ml 129.1 + 11.4 300 µg/ml 125.8 + 6.8 500 µg/ml 112.4 + 7.6 25 µg/ml 149.8 + 3.6 50 µg/ml 122.6 + 4.4 100 µg/ml 119.4 + 2.2 14 48, TGF-β1 TGF-β1 (pg/ml) Control 250.1 + 10.2 5 µg/ml arecoline 201.4 + 5.3 15 µg/ml arecoline 221.8 +9.7 30 µg/ml arecoline 177.2 + 5.1 50 µg/ml 181.7 + 6.4 100 µg/ml 155.0 + 6.1 150 µg/ml 143.0 + 1.1 300 µg/ml 154.1 + 9.7 500 µg/ml 144.7 + 1.1 25 µg/ml 221.5 + 7.1 50 µg/ml 192.1 + 18.5 100 µg/ml 166.4 + 5.0

15 24, OSF TGF-β1 TGF-β1 (pg/ml) Control 169.8 + 3.2 5 µg/ml arecoline 176.7 + 8.5 15 µg/ml arecoline 188.5 + 4.5 30 µg/ml arecoline 155.5 + 8.1 50 µg/ml 183.0 + 9.4 100 µg/ml 149.3 + 5.3 150 µg/ml 179.8 + 13.4 300 µg/ml 185.9 + 8.8 500 µg/ml 145.7 + 9.3 25 µg/ml 159.6 + 4.7 50 µg/ml 132.5 + 5.8 100 µg/ml 122.4 + 3.2 16 48, OSF TGF-β1 TGF-β1 (pg/ml) Control 255.3 + 8.9 5 µg/ml arecoline 261.7 + 6.5 15 µg/ml arecoline 282.1 +11.4 30 µg/ml arecoline 237.2 + 7.3 50 µg/ml 264.4 + 5.4 100 µg/ml 237.5 + 12.3 150 µg/ml 228.4 + 7.9 300 µg/ml 214.5 + 11.3 500 µg/ml 197.4 + 6.9 25 µg/ml 242.7 + 8.5 50 µg/ml 227.3 + 15.7 100 µg/ml 196.1 + 14.5

17 24, PDGF PDGF (pg/ml) Control 61.0 + 9.9 5 µg/ml arecoline 54.6 + 6.1 15 µg/ml arecoline 39.1 + 4.6 30 µg/ml arecoline 31.1 + 3.7 50 µg/ml 53.9 + 4.6 100 µg/ml 47.9 + 2.7 150 µg/ml 57.3 + 10.1 300 µg/ml 48.3 + 8.2 500 µg/ml 37.2 + 9.7 25 µg/ml 58.9 + 6.7 50 µg/ml 52.6 + 4.3 100 µg/ml 49.7 + 5.8 18 24, OSF PDGF PDGF (pg/ml) Control 58.3 + 8.9 5 µg/ml arecoline 56.8 + 7.2 15 µg/ml arecoline 43.5 + 6.7 30 µg/ml arecoline 30.7 + 5.3 50 µg/ml 52.1 + 8.1 100 µg/ml 45.2 + 4.8 150 µg/ml 59.7 + 8.3 300 µg/ml 41.8 + 5.1 500 µg/ml 38.1 + 3.7 25 µg/ml 59.7 + 4.3 50 µg/ml 52.9 + 6.2 100 µg/ml 47.5 + 3.8