34 3 2011 9 JOURNAL OF NANJING NORMAL UNIVERSITY Natural Science Edition Vol 34 No 3 Sept 2011 1 1 2 2 2 2 1 210046 2 215500 Porphyra katadai var hemiphylla FV MV 5 ~ 20 10 80 μmol / m 2 s 20 d Yield F v /F m 1 2 FV MV 80 μmol / m 2 s 10 μmol / m 2 s 3 MV 10 20 FV Q945 6 A 1001-4616 2011 03-0095-08 Photosynthetic Characteristics During the Formation of Reproductive Tissues of Porphyra Katadai var Hemiphylla Wang Yunxia 1 Wang Miaomiao 1 Xia Dongming 2 Zhang Tao 2 Xu Pu 2 Jiang Hongxia 2 1 School of Life Sciences Nanjing Normal University Nanjing 210046 China 2 Department of Biology and Food Engineering Changshu Institute of Technology Changshu 215500 China Abstract In this study chlorophyll fluorescence parameters Yield F v /F m and oxygen evolution were followed during the formation of reproductive tissues when female vegetative tissues FV and male vegetative tissues MV of Porphyra katadai var hemiphylla were cultured indoor 5 ~ 20 10 and 80 μmol / m 2 s for 20 days At the same time development of these tissues in culture was observed by microscope The formation of reproductive tissues was closely correlated with the decreasing of photosynthetic activities At the same temperature the tissues cultured under 80 μmol / m 2 s showed a greater extent of maturation than those under 10 μmol / m 2 s did whereas their decreasing degree of photosynthesis was also larger Under the same light intensity the extent of maturation ascended with our increasing temperature At the same time photosynthetic activity of MV culture was the highest at 10 and the lowest at 20 but the decreasing degree of photosynthesis of FV culture was smaller at higer temperature Key words Porphyra katadai var hemiphylla reproductive tissue chlorophyll fluorescence oxygen evolution Porphyra katadai Miura var hemiphylla Tseng et T J Chang Rhodophyta Protoflorideae Bangioideae Bangiales Bangiaceae Porphyra 1 2 3 4-6 2011-03-09 40906085 2010 E-mail hxjiang10@ 163 com 95
34 3 2011 7-10 11 12 PS II 13 14 15 16 17 18 12 PS II 1 1 1 2010 3 GZX-250BS - III 2 d 10 30 μmol / m 2 s 12 L /12 D FV MV 1 1 cm 2 50 ml 3 5 10 15 20 10 μmol / m 2 s 80 μmol / m 2 s LI-185B Li-COR 12 L /12 D 3 3 d 1 2 Water-PAM Walz 4 d Yield F v /F m Yield Yield = ΔF /F' m = F' m - F t /F' m 15 mins F v /F m F v /F m = F m - F o /F m 1 3 YSI 5300A 4 d 1 4 E800 Nikon 5 6 FV MV 1 5 3 6 ± n = 3 ~ 6 t- ANOVA P < 0 05 2 2 1 FV MV 1a ~ b FV 12 d 1c MV 1d 4 d 1e ~ f 20 d MV 16 d 1 96
Fig 1 1 a b c d ~ f Development of vegetative tissues of P katadai var hemiphylla in culture a initial female vegetative tissue b initial male vegetative tissue c differentiation of cells on female vegetative tissue culture d - f differentiation of cells and formation of spermatangia on male vegetative tissue culture 2 2 1 16 d Table 1 The development of vegetative tissues of P katadai 2 FV 10 μmol / m 2 s 80 μmol / m 2 s MV 80 μmol / m 2 s var hemiphylla in culture after 16 days / /μmol / m 2 s FV MV 5 10 + + + 80 + + + + Yield P < 0 05 10 μmol / m 2 s 20 d FV 5 10 15 20 39% 52% 8% 18% MV Yield 10 12 d 6% 2a ~ d 5 80 μmol / m 2 s FV MV 4 10 10 + + + 80 + + + 15 10 + + + 80 + + + + 20 10 + + + + 80 + + + + + + 10% + 10 ~ 20% d FV 4 d 20 d 37% 75% MV 10% 16% 2e 10 80 μmol / m 2 s FV 4 d 4 d + + 20 ~ 30% + + 40 ~ 60% + + + 60 ~ 75% + + + 75 ~ 90% 20 d 31% 57% MV Yield 8 d 15% 20 d 7% 2f 15 80 μmol / m 2 s FV 4 d 31% ~ 49% MV 8 d 7% ~ 10% 2g 20 80 μmol / m 2 s FV 4 d 4 d 20 d 22% 28% MV 8 d 5% ~ 19% 2h 5 10 Yield MV 8 d ~ 12 d FV 2a b e f 5 10 80 μmol / m 2 s MV Yield 8 d FV 31% 14% 20 d 23% 110% 10 10 μmol / m 2 s MV 12 d FV 36% 20 d 84% 10 80 μmol / m 2 s MV 16 d FV 33% 20 d 61% 15 20 10 μmol / m 2 s FV MV Yield 20 80 μmol / m 2 s FV MV Yield FV Yield 16 d MV 25% 20 d 53% 2h 97
34 3 2011 a - 5 10 μmol / m 2 s b - 10 10 μmol / m 2 s c - 15 10 μmol / m 2 s d - 20 10 μmol / m 2 s e - 5 80 μmol / m 2 s f - 10 80 μmol / m 2 s g - 15 80 μmol / m 2 s h - 20 80 μmol / m 2 s 2 Fig 2 Yield FV- MV- Yield of vegetative tissues of P katadai var hemiphylla in culture FV-female vegetative tissue culture MV-male vegetative tissue culture 3 10 10 μmol / m 2 s MV F v /F m FV MV F v /F m P < 0 05 5 10 μmol / m 2 s FV MV F v /F m 4 d 8 d 6% ~ 7% 20 d 23% 3a 5 80 μmol / m 2 s FV MV 4 d FV 4 d 20 d 23% 52% MV 17% 34% 3e 10 10 μmol / m 2 s FV 12 d 4% MV 3b 10 80 μmol / m 2 s 4 d FV 4 d 20 d 24% 44% MV 13% 30% 3f 15 10 μmol / m 2 s FV F v /F m 8 d 8% MV 16 d 10% ~ 16% 3c 15 80 μmol / m 2 s FV MV 4 d 17% 14% 16 d 20 d FV 43% MV 10% 3g a - 5 10 μmol m 2 s b - 10 10 μmol / m 2 s c - 15 10 μmol / m 2 s d - 20 10 μmol / m 2 s e - 5 80 μmol / m 2 s f - 10 80 μmol / m 2 s g - 15 80 μmol / m 2 s h - 20 80 μmol / m 2 s 3 Fig 3 F v /F m FV- MV- F v /F m of vegetative tissues of P katadai var hemiphylla in culture FV-female vegetative tissue culture MV-male vegetative tissue culture 98
20 10 μmol / m 2 s FV 4 d 20 d 5% 11% MV 7% 25% 3d 20 80 μmol / m 2 s FV 4 d 20 d 19% 40% MV 17% 44% 3h 10 μmol / m 2 s 5 10 15 FV MV F v /F m 20 FV MV 9% ~ 18% 3a ~ d 80 μmol / m 2 s MV F v /F m 5 8 d FV 13% ~ 38% 10 4 d 13% ~ 25% 15 20 FV MV 20 FV MV 3e ~ h 4 FV MV 15 10 μmol / m 2 s 10 10 μmol / m 2 s 15 80 μmol / m 2 s 5 10 μmol / m 2 s 16 d FV 16 d 20 d 67% 59% MV 59% 69% 4a 5 80 μmol / m 2 s 4 d FV 4 d 20 d 62% 86% MV 61% 84% 4e 10 80 μmol / m 2 s 4 d FV 4 d 20 d 64% 89% MV 64% 91% 4f 20 FV MV 10 μmol / m 2 s 12 d 80 μmol / m 2 s 20 d 1 72 4d h a - 5 10 μmol / m 2 s b - 10 10 μmol / m 2 s c - 15 10 μmol / m 2 s d - 20 10 μmol / m 2 s e - 5 80 μmol / m 2 s f - 10 80 μmol / m 2 s g - 15 80 μmol / m 2 s h - 20 80 μmol / m 2 s 4 FV- MV- Fig 4 Oxygen evolution of vegetative tissues of P katadai var hemiphylla in culture FV-female vegetative tissue culture MV-male vegetative tissue culture 10 μmol / m 2 s 5 10 15 FV MV 20 12 d FV MV 1 70 20 d MV - 596 37 μmol /g FW /h 4a ~ d 80 μmol / m 2 s 5 10 15 FV MV 20 8 d FV MV 1 85 20 d 2 84 4e ~ h 2 3 2 3 1 FV 10 μmol / m 2 s FV Yield F v /F m 5 10 15 20 10 15 5a c FV 20 d 20 10 15 5e 80 μmol / m 2 s FV Yield 20 12 d 15 > 10 > 5 5b FV F v /F m 5 15 20 5d FV 99
34 3 2011 20 5 10 15 5f 2 3 2 MV 10 μmol / m 2 s MV Yield F v /F m 10 6a c 80 μmol / m 2 s MV Yield F v /F m 20 6b d MV 20 6e f 2 4 FV Yield F v /F m 10 μmol / m 2 s 80 μmol / m 2 s 7 MV Yield F v /F m 10 μmol / m 2 s 80 Fig 7 7 a e i 5 b f j 10 c g k 15 d h l 20 FV μmol / m 2 s Photosynthetic activities of female vegetative tissue FV of P katadai var hemiphylla cultured under different light intensities 100
μmol / m 2 s 5 10 15 8 Fig 8 8 a e i 5 b f j 10 c g k 15 d h l 20 MV μmol /m 2 s Photosynthetic activities of male vegetative tissue MV of P katadai var hemiphylla cultured under different light intensities 3 5 ~ 20 45 μmol / m 2 s 5 19 20 MV 4 d 12 d FV 6 21 12 22-25 PS II 26 PS II 10 ~ 70 μmol / m 2 s 27 5 MV 20 MV 20 10 FV 5 10 μmol / m 2 s 10 ~ 15 80 μmol / m 2 s 20 MV a F v /F m 18 a 28 12 1 M 2006 1-30 101
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