梽 / 8 min min doi:.8/wst... R8. [ ] [ ] [] [6] [7] [8] [9~] [ ] [] min t /. min [] 元元元 --9 --7 ZX9-- BLP. ICR ~8 g SCXK -. nanas comosus L. Bolo Leaf Phenols BLP p - coumaric cid.% 巿 <.6% Glu 原 cose 796
绎 Vol.6 No. 7 97 BIO-RD US... 6 min h 6 mg kg -. ml/ g %. ml/ g [6 7] 6h rpm.. 6 +BLP +BLP. ml/ g BLP mg kg - BLP min rpm -8 益 Lipase U/L = min - min / stand 伊 滋 mol L - 伊 / min /mg U mg -. Microsoft Office 7 Excel 依 x 依 s One-way NOV t-test P<.. h h 6 h 6 mg kg - h h P<. 6 h mmol L - rea Under the Curve UC ~6 h UC P<. ~6 h P<. P<. B 8 6 6 6 6 BLP BLP BLP6 BLP BLP BLP6 BLP BLP BLP6 BLP P<. 797
6 6 BLP BLP BLP6 B 8 6 # B ## 6 6 # P<. P<., P<. ## P<. P<.. ~ h P<. h UC P<. BLP P<. B. min h P <. BLP min BLP min P<. P<. BLP P<. B [6 7] BLP h BLP BLP UC BLP UC 798
绎 Vol.6 No. C Normal Normal BLP BLP Normal Normal BLP BLP B Lipase D 8 6 Normal Normal BLP BLP Normal Normal BLP BLP BLP P<. h ~ h UC BLP [] BLP min min h BLP min min BLP BLP BLP BLP BLP [8] BLP BLP BLP BLP BLP 799
BLP,,..,,() 颐 ~.,,,. : RC.,,(9) 颐 8~...,,() 颐 ~.,,..,,() 颐 ~.,,,..,,() 颐 ~. 6,,,..,9,(9) 颐 ~. 7,,..,,() 颐 9~. 8. ( ). :, 999 颐 77~78. 9,,,.., 6,() 颐 ~. Ma C, Xiao S Y, Li Z G, et al. Characterization of active phenolic components in the ethanolic extract of nanas comosus L. leaves using high -performance liquid chromatography with diode array de 原 tection and tandem mass spectrometry. JChrom, 7, 6 颐 9~.,,,., HPLC.,7,(9) 颐 8~8. Xie W D, Wang W, Su H, et al. Effect of ethanolic extracts of nanas comosus L. leaves on insulin sensitivity in rats and HepG. Comp Biochem Physiol Part C Toxicol Pharmacol, 6, () 颐 9 ~. Xie W D, Wang W, Su H, et al. Hypolipidemic mechanisms of nanas comosus L. leaves in mice: Different from fibrates but simi 原 lar to statins. J Pharmacol Sci, 7, () 颐 67~7. HuJ,LinL,ShenJ,et al. Developmental toxicity of orally admin 原 istered pineapple leaf extract in rats. Food Chem Toxicol,, 9 (6) 颐 ~6. Meng Z, Wang W, Xing D M, et al. Pharmacokinetic study of p - coumaric acid in mouse after oral administration of extract of nanas comosus L. leaves. Biomed Chromatogr, 6, (9) 颐 9~ 9. 6,,,. E.,,() 颐 ~8. 7,,,. B.,9,(6) 颐 98~ 9,997. 8 Brockman H L. Lipases. Encyclopedia Biol Chem,, 颐 79 ~ 7. Effect of Phenols of Pineapple Leave on Lipid and bsorption and Intestinal Lipase ctivity after cute Consumption of Lipid and Yan Xiaojin, Chai Yushuang, Yuan Zhiyi, Lu Xi, Lei Fan, Xing Dongming, Du Lijun (Ministry of Education Key Laboratory of Protein Sciences, Laboratory of Molecular Pharmacology and Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 8, China) bstract: This article was aimed to study the antioxidant and antihyperlipidemic effect of total phenolic composition prepared from pineapple ( nanas comosus L.) leave. It had a rapid absorption after intragastric administration and the principle ingredient, p -coumaric acid, reached the peak concentration after minutes of administration. The mice model with single intragastric administration of lipid and glucose were used in the observation of changes on postprandial glucose, lipid and intestinal lipase at different time points after drug administration. The results showed that phenols of pineapple leave can inhibit triglyceride and glucose absorption in certain extent. No significant effect was observed on inhibition at minutes after the phenol administration. However, the intestinal lipase activity was obviously inhibited in normal mice and the intestinal lipase activity decline caused by acute lipid consumption can be reversed. It was concluded that the phenols pineapple leave may inhibit the absorption of lipids with correlation to lipase activity. It had certain regulation effect on the high postprandial glucose and fat absorption. Keywords: Phenols of pineapple leave, hyperlipidemia, hyperglycemia, lipase 8