,,,, 2005 2 28-1 010-51528296/97/98
- 2 010-51528296/97/98
1. t 0 t R t R t 0 t 0 NaNO 2 NaNO 3 k t R t 0 k ' k ' t 0 k t R k K k 0.4k 2030 k 25 V R t R F C ( F C --- ml/min ) V R t R t R t R t 0 V R V R V R 0 t R F C tr(2) ' t R(1) t R(2) tr(1) () t' R(2) k' (2) ( 1.1 ) t' R(1) k' (1) - 3 010-51528296/97/98
2. t R 2 N ( ) 1/2 h 2σ σ σ W 1/2 0.607 1/2 h W 1/2 ( 2t 1/2 ) W b N t 5 R.54 W1/ 2 t 16 R N Wb 2 2 W b W 1/2 L H N L H2d P ( d P 10 H20 N5 ) ( d P 5 H10 N10 ) d P 87(V/V): W 1/2 t R N 3. T f h A B 1/10h - 4 010-51528296/97/98
B T f AB A T f 1.21.3 T f 2 SiOH NH 2 () t R(1) t R(2) 2( tr2 tr1) RS W 1/2(1) W 1/2(2) t t W1 W 2 t W1 t W2 4. K 1 K 3 HPLC W 1/2 t R K 1 t K1 W R(2) 1/ 2(2) t R(1) W 1/ 2(1) K 1 H Hh K3 H K 3 1 K 3 5. L u mm/sec L t 0 H(m) t 0 h A u1 mm/sec A u(1mm/sec) - 5 010-51528296/97/98
5 mm 1.0 ml/min 1 mm/sec 2 mm 0.2 ml/min 1 mm/sec 1 mm 50 L/min 1 mm/sec 1 mm/sec 5mm 1mm 6. lnk ablnc b cc b C b lnk acc b abc lnk ablnc b lnk lnk C b C b lnk lnk / D / C b D C b (/) D Dk lnk lnk C A B C b A A D A C b - 6 010-51528296/97/98
A B C k B A D C b C b k C b HPLC 1090/ 30min C b C b C b 10k 23-7 010-51528296/97/98
1 2 3 SFE 90% 50% ( 99.5%) 5ppm 3% FDA Food Additive Regulation 21CFR173.65 2000 2 17 [2000]56 Amberlite XAD HP - 8 010-51528296/97/98
SEC Sephadex LH-20 Toyopearl 1 () ()() 2 3 ph 4 ( )()() 11 80%- 90%- C18 C18 MCI GEL MCI MCI MCI GEL MCI GEL Amberlite FP 5-200mg TSK Toyopearl HW-40 Sephadex LH-20 Toyopearl HW-40-9 010-51528296/97/98
Petunia integrifolia 1h 5 50 C18 20mL 180mL 1 / 2 PVPhide powder 3-10 010-51528296/97/98
1 2 100200 3 1 2 35mm 3 4 5 6 1 TLC TLC 2 3 4-11 010-51528296/97/98
V ( cm / s) ( ρ ρ ) 2 2gd = 3600η g cm/s2d cmρg/cm3ρo g/cm3ηmpa.s ρρo, o 1 LiChrospher 100 2.3g/cm3LiChrospher 500 2.46g/cm3LiChrospher 1000 2.49g/cm3 2.2g/cm3 (ρ/g cm -3 ) η 3.3 29 (mpa.s 20 o C) 3.0 2.5 10 2.3 5 1.6 10 1.5 6 1.11 17 0.8 30 0.8 23 0.8 12 2 3-12 010-51528296/97/98
YMC-Pack AA12S50 C18 Millipore Vantage V2 1 50m 12bar 7bar 2 YMC S50 D40/D90 1.33 3 4 2040 5 Toyopearl Amberchrom & MCI GEL 1 2 12cm 3 4 5 6 7 8 9 10 11 12 8 13 14 1 2 100 150cm/h 810 CV 3 80/2070/30 50/50 40cm/h 2 4-13 010-51528296/97/98
Sephadex LH-20 Sephadex LH-20 G-25 Pharmadex LH-20 Sephadex LH-20 25100m 250cm Sephadex LH-20 1 2 75% 25% 3 1.5ba 0.45um 1-2% 0.45um 12cm/min 1.5ba 1-10cm/h 2-3 - 14 010-51528296/97/98
=/ Sephadex LH-20 =/ Sephadex LH-20 4-5KD 20% 1% 18-111um 70um 27-163 um 103 um 720cm/min 60 cm/min ph 2-11 2-13 ph2 121 20 4 40 4-25 4-8pH6-8 20%0.04% - 15 010-51528296/97/98
ml /g Dimethyl sulphoxide 4.44.6 Pyridine 4.24.4 Water 4.04.4 Dimethylformamide 3.84.2 Saline 3.94.1 Methanol 3.84.1 Methane dichloride 3.84.1 Chloroform 1 3.84.1 Propanol 3.74.0 Ethanol 2 3.63.9 Isobutanol 3.63.9 Formamide 3.63.9 Methylene dichloride 3.63.9 Butanol 3.53.8 Isopropanol 3.33.6 Tetrahydrofuran 3.33.6 Dioxane 3.23.5 Acetone 2.42.6 Acetonitrile 3 2.22.4 Carbon tetrachloride 1.82.2 Benzene 1.62.0 Ethyl acetate 1.61.8 Toluene 1.51.6 1 1 2 1 3 2.5mL/g - 16 010-51528296/97/98
Toyopearl Toyopearl TSK-GeL 5PW ph I 1 a Toyopearl TSK-GEL 5PW Toyopearl 0.53bar745psiTSK-GEL 5PW 310bar 45140psi Toyopearl TSK-GEL 5PW Toyopearl 5barTSK-GEL 5PW 20bar Toyopearl TSK-GEL 5PW HPLC PEEK btosoh 1 4 3 4-17 010-51528296/97/98
4 5L 78L 6570 20L 2Toyopearl TSK-GEL 5PW Toyopearl Coarse C : 100µm 15-30 Medium M : 65µm 30-45 Fine F : 45µm 45-60 Superfine S : 35µm 60-90 TSK-GEL 5PW 30µm 90-120 20µm >120 3 4 3 5 3 3-18 010-51528296/97/98
c) Toyopearl TSK-GEL TOSOH HIC I SEC Toyopearl HW-40, HW-50, HW-55,HW-65 0.1M Na 2 SO 4, NaNO 3, NaCl 50mM HW-75 Tris IEC Toyopearl DEAE, QAE, SuperQ, CM,SP 1M NaCl 50mM,Tris, Toyopearl MegaCap SP-550 TSK-GEL DEAE-5PW, SuperQ-5PW,SP-5PW HIC Toyopearl Ether, Phenyl, Butyl,Hexyl-650, 2M Na 2 SO 4, (NH 4 ) 2 SO 4 NaCl 50mM PPG-600, Super Butyl-550 TSK-GEL Ether-5PW, Phenyl-5PW AFC 0.5M NaCl 0.1M NaHCO 3 Toyopearl AF-Tresyl AF-Epoxy-650 Toyopearl AF-Formyl-650, AF-Amino-650, 1M NaCl 100mM NaHCO 3 AF-Carboxy-650 Toyopearl AF-Chelate-650, AF-Blue HC-650 0.5M NaCl 0.2M 20mM AF-Red-650 Tris d) 1 2>12 3 Toyopearl 3050 TSK-GEL 5PW 2570 4050 4-19 010-51528296/97/98
Toyopearl HW-40, HW-50, HW-55, HW-65, HW-75F Toyopearl Ether-650, Phenyl-650,Butyl-650, Super Butyl-550,Hexyl-650, PPG-600, DEAE-650,SuperQ-650, CM-650, SP-650, TSKgel DEAE-5PW, SuperQ-5PW, SP-5PW, Ether-5PW Phenyl-5PW Toyopearl QAE-550C, SP-550C, Toyopearl MegaCap 1.1 1.2 1.25 1 2 31g 1mL 4 3050-20 010-51528296/97/98
2 µ µ µ µ µ - 21 010-51528296/97/98
5L 010-5152 8297 / 1 2 12cm 3 4-22 010-51528296/97/98
5 6 7 8a 8b Toyopearl 3bar44psi 9 10 11 23cm - 23 010-51528296/97/98
12 8 13 14 15 1214 0.5cm 23 16 Toyopearl - 24 010-51528296/97/98
1 50 2 3 4 50 5 6 23cm 7 8 9 10 11 / 12-25 010-51528296/97/98
13 14 1013 0.5cm 15 3 Toyopearl 510 0.251 B12 50250cm/hr 0.8 1.4 TSK-GEL 5PW 20mm 30mm TSK-GEL 5PW Toyopearl TSK-GEL 5PW Toyopearl 650 1000 ÅTSK-GEL 5PW Toyopearl DAC 3bar TSK-GEL 5PW 310bar 15 20bar As < 0.8 As > 1.4-26 010-51528296/97/98
SEC IEC HIC AFC Toyopearl S F M I.D.(cm) (/) (/) (/) C (/) 2.2 5000 3500-3000 5.5 5000 3300 - - 10.8 5000 2500 - - 21 4000 2200-1500 31-2000 - 1200 40-1800 - 1000 2.2 6000-4000 2000 5.5 6000-4000 - 10.8 6000-4000 - 21 4000-2600 2000 31 - - 2000 1000 40 - - 1500 750 2.2 6000-4000 2000 5.5 6000-4000 - 10.8 6000-4000 - 21 4000-2600 2000 31 - - 2000 1000 40 - - 1500 750 2.2 - - 4000-5.5 - - 4000-10.8 - - 4000-21 - - 2600-31 - - 2000-40 - - 1500 20µm 30µm I.D. (cm) (/) (/) 5.5 1500 10.8 1500 IEC 15.8 1200 21 1000 DAC* ** 20-30 10000 7000 5.5 1500 HIC 10.8 1500 15.8 1200 21 1000 DAC* ** 20-30 10000 7000 *DAC = Dynamic Axial Compression >3bar ** = >0.5M NaCl - 27 010-51528296/97/98
II 1 a) SEC I510 Toyopearl HW 13 / 1020 b) IEC 510 ph 4.8-5.2 4.2-5.2 MES 5.5-6.7 6.7-7.6 HEPES 7.6-8.2 L- 5.5-6.0 6.6-7.1 7.3-7.7 Tris-Cl 7.5-8.0 8.4-8.8 ph c)hic 1M3M - 28 010-51528296/97/98
ph HIC ph 8 HIC (1.5M 25C) HIC Triton X-100 Tween 20 SDS CHAPS Emulgen 911 CTAB Lubrol PX 310 ph d) AFC Toyopearl AF RedAF Blue-HC 35 Tris 1M 2M 4M 20mM ph7.5 35-29 010-51528296/97/98
2M 3M 1 2M KCl 3M NaCl 2 1% Triton X-100 / 1M NaSCN / 75% / 4M 0.1M NaOH / 4.2M () 10mM 2 Toyopearl TSK-GEL 5PW 3 SEC 1 0.51M 0.10.5M 0.10.5M Toyopearl Toyopearl 2 1020 IEC 0.51M 0.10.5M 0.10.5M DEAE QAE 0.10.5M 0.10.5M 0.10.5M HIC 1-30 010-51528296/97/98
0.51M 0.1 0.5M Toyopearl TSK-GEL 5PW 2 1020 AFC 2 0.5M AF-Heparin 3 SECIEC HIC 20 4 25 C AFC 1M 20 410 C AF-Formyl 650M 1M 1%gluteraldehyde 410 C 4// Toyopearl TSK-GEL 5PW 121 C 20 200ppm 12 Toyopearl TSK-GEL 5PW ph212 <12 ph0.5n NaOH 0.5NNaOH 4 3 4 Toyopearl TSK-GEL 5PW 20-31 010-51528296/97/98
5-32 010-51528296/97/98
Amberchrom MCI GEL Amberchrom MCI GEL Amberchrom CG161CG300 CG1000 MCI GEL CHP20P - Amberchrom CG71 MCI GEL CHP2MGY 25mL 50L Amberchrom 010-5152 8297 1 Amberchrom 0.510bar7150psi Amberchrom 20 1020 0.1TFA 2 1 2 3 4 5 6 124 3 5065 1 2-33 010-51528296/97/98
3 4 5 /100 6 1.1 7 5065/ Amberchrom 1 23 2 3 Millipore E.Merck Amberchrom TechniKrom ProChrom Amberchrom 4 "S" 1015 mm "M" 3040 mm "C" 5070 mm 5-34 010-51528296/97/98
23 2 1Amberchrom (cm ID cm) (ml/min) (ml/min) 1.0 10 S 4.0-10 0.2-4.0 2.2 30 S 15-25 1.0-10 7.0 100 S 100-200 10-50 1.0 10 M 5.0-10 0.2-5.0 2.2 30 M 15-40 1.0-15 7.0 100 M 100-300 10-100 1.0 10 C 7.5-15 0.2-10 2.2 30 C 20-60 1.0-30 7.0 100 C 100-400 10-200 1 3 2 12cm 4-35 010-51528296/97/98
3 5 4 6 5 7 6 8 9-36 010-51528296/97/98
7 8 (cm ID x cm) 2 Amberchrom (ml/min) (ml/min) (min) 1.0 x 10 CG71c 15 5 0.5 2.2 x 30 CG161m 40 10 1 7.0 x 100 CG1000s 200 40 2 9 10 11 11 10 12 8 13 14 DAC 1 11-37 010-51528296/97/98
2 12cm 3 5 4 12 5 6 DAC 8 10bar 13 14-38 010-51528296/97/98
Amberchrom CIP 1 2 100 150cm/h 810 CV 3 45/5550/50 50/50 40cm/h 5 4 1 35 25% /75% Amberchrom CG1000s 35%/65% Amberchrom CG71 CG300 45%/55% Amberchrom CG161 1mg/ml55 40cm/h 0.51 0.81.6Amberchrom 3 3 Amberchrom (cm) S (/) M (/) C (/) 1.0 5000 2500 1000 2.2 2500 1500 750 7.0 1500 1000 500 35 Amberchrom TFA Amberchrom 26 (2~3%) 100 1 Amberchrom 0.5M - 39 010-51528296/97/98
NaOH 1218 / 10010 4 Amberchrom 2 20 4 C25 C 3 Amberchrom 15a 15b - 40 010-51528296/97/98
1 2 0.5 20 3 4 2 2BV 2BV/H 5 2.5-5BV 2BV/H 6 a 10 30 b 200400nm 250nm 7 2BV/H 8 2BV4% HCL 5BV/H 3 ph ph=7 9 2.5BV 5% NaOH 5BV/H 3 ph ph=7 10 3ml 10ml 15-41 010-51528296/97/98
XAD BV/h 1-5 5-10BV 1-5 5-10BV ph 0.5-3 1-5 0.5-1BV 0.5-3 2-10BV ph 1-5 3-4BV ph 0.5-3 3-4BV 75+ 1-5 3-4BV 1 3-42 010-51528296/97/98
1 HPSP HP-MG 2 HP (1) (Cap )SP200 >HP SP800900 >HP-MG SP200 SP200 HP-MG SP200 2 SP200>HP-MG >HP SP800900 3 HP-MG<SP200 <HP / 2030 HP-MG 10SP200 20 4 SP800900 >HP HP-MG SP800900 3 HP SP - 43 010-51528296/97/98
HP SP NaOH NaOH SV0.52 SV0.52 LV 0.2 0.7HP HP LV 5 8 SP SV0.52 SV0.52 SV15 HP SP SV0.52 [] 50+1N NaOH SV0.51 SV15 SVSpace Velocity LVLinear Velocity BVBed Volume - 44 010-51528296/97/98
(H&E Co.,Ltd.) TOSOH Develosil YMC Amersham MERCK Millipore Varian Applexion Sigma-Aldrich - 45 010-51528296/97/98
719 010-5152 829682978298 010-5152 8299 Emailsales@herbs-extract.com Web www.herbs-extract.com 102208