中国实验动物学会团体标准 实验动物肿瘤坏死因子 α 转基因关节炎小鼠模型饲养繁育规范 编制说明 联系人 : 李舸 联系电话 :15011983691 编制单位 : 广东省实验动物监测所 时 间 : 2016 年 10 月 25 日
中国实验动物学会团体标准 人肿瘤坏死因子 α 转基因关节 炎小鼠模型饲养繁育规范 编制说明 GB/T1.1 2009 1 2011A081401005 2013B040400007 1. 2011 2011A081401005 2013B040400007 [1] 3 hutnf 3 2.8kb hutnf hutnf TgTC 100% 3 1A1B TgTC 10 1C 1D TgTC
/20 1E 1F TgTC 1 TgTC FVB A 3 C 10 E 20 TgTC 3 D 10F 20 hutnf Adalimumab 2B Infliximab 2C AT132 2A TgTC hutnf 3 hutnf TgTC
2 hutnfa TgTC A AT132 B Adalimumab C Infliximab 3 hutnfa 8 A TgTC B C AT132 1.25mg/kg 3 8 8 hutnfa D [2]
2. 2015 12 3. Rheumatoid arthritis, RA 3.55 1 0.2-0.4% 90 (Human tumor necrosis factor, hutnf ) RA RA TNF FDA SFDA CDE 2012 2013 TNF 11 TNF RA 90 hutnf Tg197 RA TNF Tg197 TNF 1 2
3 1. RA [1] RA 3 3 -UTR 3 2.8kb BamHI Sall 2.8kb TNF/ globin FVB 4 4 F4 5 100%
3 10 20 5 F4 2. SPF GB 14925-2010 SPF 3. SPF GB 2010 14925- SPF 4. GB 2010 14924.3- GB14924 2001
5. 100% PCR 6. 3 10 20 [3] 7. 7.1 7.2 [4]
-2010 7.3 7.3.1 [5] 7.3.2 [6] 8. 5 4 3-5 Jackson GENETIC STABILITY PROGRAM [7] 5 F1 9.
9.1 10 6 [6] 9.2 FVB 4-6 9.3
1. Keffer, J., et al., Transgenic mice expressing human tumour necrosis factor: a predictive genetic model of arthritis. EMBO J, 1991. 10(13): p. 4025-31. 2. Li, G., et al., Establishment and evaluation of a transgenic mouse model of arthritis induced by overexpressing human tumor necrosis factor alpha. Biol Open, 2016. 3. Williams, R.O., M. Feldmann, and R.N. Maini, Anti-tumor necrosis factor ameliorates joint disease in murine collagen-induced arthritis. Proc Natl Acad Sci U S A, 1992. 89(20): p. 9784-8. 4. Hayward, M.D., et al., An extensive phenotypic characterization of the htnfalpha transgenic mice. BMC Physiol, 2007. 7: p. 13. 5. Nakao, K., N. Nakagata, and M. Katsuki, Simple and efficient vitrification procedure for cryopreservation of mouse embryos. Exp Anim, 1997. 46(3): p. 231-4. 6. Nagy, A., Manipulating the mouse embryo : a laboratory manual. 3rd ed. 2003, Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press. x, 764 p. 7. Taft, R.A., M. Davisson, and M.V. Wiles, Know thy mouse. Trends Genet, 2006. 22(12): p. 649-53.