チャ害虫総合管理のための薬剤抵抗性ケナガカブリダニ Amblyseius womersleyi の利用に関する研究 誌名 野菜茶業研究所研究報告 ISSN 13466984 著者 巻 / 号 望月, 雅俊 2 号 掲載ページ p. 93-138 発行年月 23 年 3 月 農林水産省農林水産技術会議事務局筑波産学連携支援センター Tsukuba Business-Academia Cooperation Support Center, Agriculture, Forestry and Fisheries Research Council Secretariat
Pesticide application to A. womersleyi by rotary spray tower (see, photo) A kidny bean leaf infested with T. urticae Water sokaed cottons A. womersleyi Rotary spray tower (manufac. by Mizuho Scientific Co. LTD.) Water Agar gel (.5%) A sprayed leaf was transferred onto the fresh leaf inested with spider mite. The survivors on both leaves were checked at 48hr after application
No. of adult females of T. kanzawai /leaf 2.5 2 1.5 1.5 Mortality(%) with Fenpropathrin(1% EC) May Jun. Jul. Aug. Sep. Oct. 1 5 (63) Synthetic pyrethroid application T. kanzawai A. womersleyi 1ppm 5ppm (82) (75) Apr. May Jun. Jul. Aug. 1991 1992 91/7 91/9 91/1 92/7 (64) (78) (47) (66).25.2.15.1.5 No. of adult females of A. womersleyi / leaf
Mean survival rate (%) 1 5 Permethrin 2% WP 4 ppm 1 2 3 4 5 6 7 8 9 1 Selection Permethrin 2% EC 5 ppm
LC 5 (ppm ) 1 1 1 1 2 3 4 5 Months
% Mortality 1 8 6 4 2 1 8 6 4 2 1 8 6 4 2 a) Hiranuma-2 5 1 15 2 25 3 b) Kanaya94 5 1 15 2 25 3 c) Kikugawa 5 1 15 2 25 3 Days after collection from the tea fields Cypermethrin 6ppm Fenvalarate 1ppm Fluvalinate 2ppm Permethin 1ppm Fenpropathirn 5ppm Permethrin 1pm Fenpropathrin 1ppm Permethrin 4ppm
Control plot (C) 3.3m N Released plot (R) Tea bush 1.5m 5m.5m Screen house Screen house 5m Sampling areas for new leaves Observation areas for mites
No. of adult females of T. kanzawai Resistant strain 3 R-1 permethrin (WP) 2 T. kanzawai 1 A. womersleyi May June July 3 R-2 permethrin (WP) 2 T. kanzawai A. womersleyi 1 May June July 1 R-3 permethrin (WP) 3 3 2 2 1 1 Susceptible strain S-1 3 permethrin (WP) 2 T. kanzawai A. womersleyi 1 May June July 3 3 S-2 3 permethrin (WP) 2 2 2 T. kanzawai A. womersleyi 1 1 1 May June July 1 1 S-3 1 permethrin (WP) No. of adult females of A. womersleyi Released plot (R) No. of adult females of T.kanzawai 5 4 3 2 1 5 4 3 2 1 permethrin A. womersleyi 2 2 * * * * * * * * * * June July Control plot (C) permethrin.2.1.2.1 No. of adult females and nymphs of A. wormersleyi / leaf 5 T. kanzawai A. womersleyi 5 5 T. kanzawai A. womersleyi 5 May June July May June July June July
Covered area Uncovered area N SP Control Conventional control SP Control Conventional Control L1 H2 Tea hedge Observation area 2m H1 2.7m.75m 2m Damage of new leaves -1% 1-2% 2-4% 4-6% 6%-
No. of adult females of T. kanzawai / leaf or shoot (a) 1) Covered area Synthetic pyrethroid control Matured leaves 2.5 * 2 1.5 1.5 2) Uncovered area New shoots.2 (b) Conventinal control (a) Synthetic pyrethroid control.5.5 (b) Conventinal control 1.1 1.8 6/16 6/21.8.8.6.4.2 May 15 * May 15 * * May 3 * May 3 *.1 6/16 6/21 June14.1 * June14 June29.1.25 June29 *.2.1.2.15.1.5.6.4.2 2.5 2 1.5 1.5.6.4.2 May 15 May 15 May 3 May 3.2.1.2.1 6/16 6/21 June14 6/16 6/21 June14.2.1.25.2 June29.15.1.5.2.1.1.8 June29.6.4.2 No. of Adult females of A. womersleyi / leaf or shoot
Number of adult females of T.kanzawai / leaf 5 4 3 2 1 5 4 3 2 1 May19 June8 June3 5 4 3 2 1 5 4 3 2 1 5 4 3 2 1 Synthetic pyrethroids application May19 June8 June3 May19 June8 June3 May19 June8 June3 May19 June8 June3.5.4.3.2.1.5.4.3.2.1.5.4.3.2.1.5.4.3.2.1 May19 June8 June3 (a) Covered area % of damaged new leaves 1 8 6 4 2.1 (b) Uncovered area % of damaged new leaves.2 1 3 4 5 2 Peak density of A. womersleyi 2 15 1 5.3.2.4.6.8 Peak density of A. womersleyi.4.5.4.3.2.1 Days J J J 1.2 5 4 3 2 1 5 4 3 2 1 5 4 3 2 1 5 4 3 2 1 May19 June8 June3 May19 June8 June3 May19 June8 June3 5 4 3 2 1 6 7 8 9 1 May19 June8 June3 6 5 4 3 2 1 1 1 8 6 4 2 Peak density of T. kanzawai.8 J.6.4.2 2 15 1 5 Peak density of T.kanzawai % of damaged new leaves % of damaged new leaves.5.4.3.2.1.5.4.3.2.1.5.4.3.2.1.5.4.3.2.1.5.4.3.2.1 Number of adult famales of A. womersleyi / leaf Damage rof new leaves (%) 9 8 7 6 5 4 3 2 1 r=-.52, p<.1.1.2.3.4.5.6.7.8.9 Predator / prey density ratio
Number of individuals captured by beating method 5 4 3 2 1 5 4 3 2 1 Covered area (a) Yellow tea thrips (S. dorsalis ) (b) Tea green leafhopper (E. onukii ) 1 Insecticide application Insecticid applicatin May 2 May 3 June 9 June 19 Days Uncovered area (a) Yellow tea thrips (S. dorsalis ) Insecticide application June29 5 May 2 May 3 June 9 June 19 1 5 Days (b) Tea green leafhopper (E. onukii ) Insecticide application May 2 May 3 June 9 June 19 June29 May 2 May 3 June 9 June 19 June29 June29 Days Days
Yield(g) within quadrat (2 x 2cm) (a) Covered area 25 2 15 1 5 1 2 3 4 (b) Uncovered area 25 2 15 1 r=-.3, p>.5 r=-.14, p>.5 5 1 2 3 4 Damage degree index of new leaves % of new leaves damaged on June22 1 8 6 4 2.3.6 Density of A. womersleyi on May 31.9.12 1 6 5 4 3 2 1 8 6 4 2 Density of T. kanzawai on May 31 % of new leaves damaged on June22
Damage of new leaves (%) 9 8 7 6 5 4 3 2 1 5 Infestation rate 1% 2% 4% r=.69, p<.1 4% 2% Damage 1% Damage of new leaves (%) 7 6 5 4 3 2 1 5 r=-.56, p<.1 5 1 2 3 4 5 6 7 8 9.5.1.2.3.4.5.6.7 Infested rate (%) of mature leaves (%) Proportion of predator / prey