52D 2 () Vol.52 No.2 2013E3 JournalofXiamenUniversity (NaturalScience) Mar.2013 doi:10.6043/j.issn.0438 0479.2013.02.023 234 56)*+`a 1,2,,@ 2, 1, 1 (1. % *77 :; GHI/$JK, 361005;2., 352100) -.: L [ Q! R, N " 0 a D!. :;7 (10,100,1000ng/L)! D)*+( 犛犲犫犪狊狋犻狊犮狌狊犿犪狉犿狅狉犪狋狌狊 )A 50d! F (,GH D)*+,Z 6-. '!.8 <,) * +, Z! (GSH) S C / (GST) 6 (GSHPx) 3c (MDA) Na + K + ATPase]7 ' 4Q : 7! 6, T 6.! (D)*+! 6-.! U.$J!8 <C, ] *! QD ] F:; U '. /01:)*+; ; 6-. : X174 234: A 5# : 0438 0479 (2013)02 0267 06 ]1970 E. U! (triazophos) S 6 [1], * & E!. ", a J,1 Q C +! 4 Q B a 4 ) [ R ] a Q [2].1! (pa clobutrazol) 1 F ; R < Q < [3 4]., *! Q! : ;, 3 : (propiconazole) 4 F (ketoconazole) $! 9 = W W ` [5], 3 : ` R $ 0! J $! 0.15~13μg/L [6].D R, 78$ G H a! 103 J *, *N 12 J,1! a 119.6 ng/l [7].!", 6-. (antioxidant system)4 I ^! _ > ' N \ [8 9], ( 1 6-.!7 678:2012 10 08 9: ; := ] / " (20100121110005); ] (2012D114); 9 7 7 I / (2011H309) :cgwang@xmu.edu.cn ' [= b23>f! 6 U>F [10 11]. F 6 5! 6(!>F 6 : _, 6-. LX!7 5H! ' [12].N *D ]! 4 Q,Li] [13 14]!" 3:! ( ^ 6 -. ethoxyresorufin O deethylase (EROD)] 6 '! 6.Q 0.2,50 500 μg/l!3: D ( 犗狀犮狅狉犺狔狀犮犺狌狊犿狔犽犻狊 )A (,1 Z 6 RNA DNA! I 0! 6 ': a [15]. D*L ( 犇犪狀犻狅狉犲狉犻狅 )96h! ^ ( 犔犆 50)L 20.55mg/L, *L 6 (CAT) 6 6 (SOD)!5 a! [16].!",H )*+( 犛犲犫犪狊狋犻狊犮狌狊犿犪狉犿狅狉犪狋狌狊 ) & 50d :;7! (10,100, 1000ng/L) (,1 >! a2", U >, 6 % R,T!@G( L >!^,T ( T F $ [17], N D 6-.! " \.$J G* 78 Q!& )*+ DZ, :;7! A (, D 6 '!, D! Q( U!! 0! 9 Q.
268 % (] ) 2013E 1 1.1 7 Y 1.1.1 7 ( S :76738 62 0),/][7 N, * K % 95%. Q Q c (DMSO) 5,50 500 μg/ml!.1 6 *SaG!_ K. 1.1.2 Y (GSH) S C / (GST) 6 (GSHPx) 3 c (MDA) Na + K + ATPaseV a/] U " ; QL` G250 [7 N S. 1.2 < 8 <+' )*+/],OQF 25~35g,F 10~15cm! b F F ] A ( $ J. ($J,)*+ UV Q" 23~ 24!$ 7 A 7d! 6. ($J $JQ _4 (# D,10,100,1000ng/L ),9 ( F @50L, > )*+25((.a9( 2L F),PQ" 23~24!$ 7 A ($J;10,100 1000ng/L _ R V 100μL 5,50,500μg/mL!, D RV]! DMSO, _ N M. ( $ J 9 I!7 (25L), 23I!* ;9 2h! 9E (E /] ), UV & E L2.9 7d I 7 *. ( 50d. ( $ J 8 T Z 1, Z, # Q 9! -80 O 5Q. 6 ( aa 3,$ 7 Q&3_! R ". 1.3 < >: G Sigma S, 3 18K; (Q_ `:#, UV2550. 1.4 < GSH GSHPx GST MDA Na + K + ATPase H V! 4A. H 7! ', Z6. ( 狀 =6)A 7 '!H. 1.5 *+ $J8<Q.a ±' (mean±se), $JD Q Spss17.0 A `a _, ID FPQb _ Duncan s4 _ D!, 狆 0.05. 2 H 2 1 234 犌犛犎 + $J8< 1, (,)*+, Z GSH b!c, GSH D* b.1 ;!10ng/L D F b ( 狆 >0.05),!100ng/L =!1000ng/L GSH afd b ( 狆 <0.05).D GSH (3.281±0.226)mg/g,10 ng/l GSH (2.955±0.478)mg/g,100 ng/l GSH (1.887±0.186)mg/g,1000ng/L GSH (1.654±0.317)mg/g. 1 (D)*+,Z GSH! Fig.1 Efectsofpaclobutrazolexposure ongshcontentinthespleenof 犛. 犿犪狉犿狅狉犪狋狌狊 2.2 犌犛犜 + )*,Z GST 5! 6 c GSH 7 5 ( 2), GST D* b.1,d GST 5 (9.31±3.34)U/mg,; GST 5 (8.71±2.21)U/mg, GST 5 (7.39±2.60)U/mg, ; GST 5 D F a b ( 狆 >0.05), = GST 5 (5.35±1.90)U/mg, D F b( 狆 <0.05). 2.3 犌犛犎犘狓 +! (D)*+,Z GSHPx5! # Z.$J8<,D GSHPx5 (117.86±30.12)U/mg,; GSHPx5
2 A ]: (D)*+,Z 6-.! 269 2 (D)*+,Z GST 5! Fig.2 EfectsofpaclobutrazolexposureonGST activitiesinthespleenof 犛. 犿犪狉犿狅狉犪狋狌狊 4 (D)*+,Z MDA! Fig.4 Efectsofpaclobutrazolexposureon MDAcontentinthespleenof 犛. 犿犪狉犿狅狉犪狋狌狊 (89.16±10.18)U/mg, D F GSHPx 5 b ( 狆 >0.05); = GSHPx 5 _ (68.23±8.83) (59.13±15.84)U/mg,aFD b ( 狆 <0.05).( 3) 2 5 犖犪 + 犓 + 犃犜犘犪狊犲 + ( )*,Z Na + K + ATPase5 6 5. $J8<(, ( )*,Z Na + K + ATPase5 #! c. 1 D Na + K + ATPase 5 (1.82±0.59) U/mg,;!10ng/L Na + K + ATPase5 (1.21±0.29)U/mg;!100ng/L =!1000ng/L! Na + K + ATPase5 _ (1.29±0.36) (0.96±0.39)U/mg, afd b( 狆 <0.05). 3 (D)*+,Z GSHPx5! Fig.3 EfectsofpaclobutrazolexposureonGSHPx activitiesinthespleenof 犛. 犿犪狉犿狅狉犪狋狌狊 2.4 犕犇犃 + 4L (,) *, Z MDA! 6 c. (,)*+,Z MDA 6 [=!c, MDA D* [=.1 D MDA (3.19±1.30)nmol/mg,;! 10 ng/l MDA (4.26±1.42) nmol/mg, D F MDA = = ( 狆 >0.05), 100ng/L 1000ng/L! MDA _ a(6.12±1.89) (6.16±2.24)nmol/mg, a = D ( 狆 <0.05). 5 (D)*+,Z Na + K + ATPase5! Fig.5 Efectsofpaclobutrazolexposureon Na + K + ATPaseactivitiesinthespleenof 犛. 犿犪狉犿狅狉犪狋狌狊 3 GSH L 6 W!A
270 % (] ) 2013E 6,(R F ]!.W, 6 5 ]! 3 I^4Q;GST J6 GSH `& 89, 6! `, )! [18].DiGi ulio] [19] \,GSH (! ( A, (! 4Q. T ] [10]! ",) * (TBT) (7d 1 Z GSH, ( 50d #, T J # TBT b 7,TI8< b F GSH A #!%/. $J,* )*,Z GSH #,C )*,Z GSH &! ( A, L Z, *!==,GSH A I - #.TL * 6! 4Q, 6 9 U! GSH * ^! GSH, ^ 1 b.$ J 8 <, GSH 7! 6 GST GSHPx5! 6 b! c, * b.gst!^ Y I B L J 6 GSH! ( SH)8 9 a 69 [! 69 U N [20]. GSHPx ( GST!J64Q, 0! F89, b;,r 4 6, ) 5 6 (H 2O 2 ) 0 ] ( OH)! I ^ 4 Q [21].,GST GSHPx5! b! 4Q )* +DF!N!b b.!",) * +! (,1 Z T GSH GST GSHPx! 6 N 5! 6 c [22],C! (D)*+T! 6-. a U. MDA L] b.!,(!; 4Q,23 _> _ >, ^ 6 [23], 1! = ; / >F 6 a] b! [11].N! 1 ZM, MDA! 6 N \, Z c] [24]! \,1A ( 犗狉犲狅犮犺狉狅犿犻狊狀犻犾狅狋犻犮狌狊 ) & 60d! (,1 Z MDA [=c,t ] [10]! \,)* TBT & 50d! (, Z MDA : [=c. $J,)*,Z MDA! 6 [O8< 5.MDA 4 6 6!,1! 6 / >F 6 ] b! [25], F a,> F MDA,T C! ( D ) *,Z 6 U. Na + K + ATPaseL U Na + /K + 5!^ _, J b! M,;R L.!U_,.[1 ]U_4 /! $ Na + K + ATPase!5 [26]. Na + K + ATPaseD!, U : ; ( >F >7!I^ YI [27]. $J, * )*,Z Na + K + ATPase #! Z. = ] [28]! ", D*L ( 犇犪狀犻狅狉犲狉犻狅 ) Na + K + ATPase! 5 ; : 5 = #! Z,TL ;! 4 Q F(A ], Na + K + ATPase5 ; =, 4Q!, F 6, 6,>F,Na + K + ATPase5.Ob;, (! R,5 ;.N! " J Na + K + ATPaseD R a N, : N I! [9,29 31]. $J, )* & (,1>F &! 4Q,,, Z Na + K + ATPase5 Bb;,TI Z!. 4 H D ( )*+,Z 6-. ^ '!GH,! (D)*+! ^ 6 'MN! #4Q,! (D)*+! 6-. U, TJ!.> F 6 5! 6 >F 5 % R, " >F 6-.!b [32],! ( $ I 2 " H ) * +! >,R 4 0 6'! γ C 5 b [17]. [N" D K; * [33], TJ ; * F:;!,D ] : ; U!. IJ : [1] Hilmar M,OtoS.Phosphoricacidesters:US,3686200
2 A ]: (D)*+,Z 6-.! 271 [P].1972 08 22. [2]!,M,],]. *" A B[J]. % :],2003,20(1):91 96. [3] Singh V K,Bhatacherjee A K.Genotypicresponseof mangoyieldtopersistenceofpaclobutrazolinsoil[j].sci Hortic,2005,106:53 59. [4] Srivastav M,RamS,SharmaR R,etal.Residualefectof paclobutrazolonyieldandfruitqualityofmangocultivars [J].IndianJPlantPhysiol,2003(SpecialIssue):483 488. [5] vandesteenejc,lambert W E.Validationofasolid phaseextractionandliquidchromatography electrospray tandem massspectrometricmethodforthedetermination ofninebasicpharmaceuticalsin wastewaterandsurface watersamples[j].jchromatogra,2008,1182:153 160. [6] CastiloL E,MartinezE,RuepertC,etal.Waterquality and macroinvertebrate community response folowing pesticideapplicationsinabananaplantation,limon[j]. CostaRicaSciTotalEnviron,2006,367:418 432. [7] %. 0 7 8 : ; 103 J * >701 -X" ([D]. : %,2009. [8] WangCG,ChenYX,LiY,etal.Efectsoflowdosetrib utyltinonactivitiesofhepaticantioxidantandphase I enzymesin 犛犲犫犪狊狋犻狊犮狌狊犿犪狉犿狅狉犪狋狌狊 [J].Bul Environ Contam Toxicol,2005,74(1):114 119. [9],X,S 7._ > " A B 0 1 :;O! -[J].,1995,19(2): 172 184. [10] T,, b ',]. (TBT)D ) * Z 6! [J]. % :], 2008,47(5):748 751. [11],,S,]. J * D 1 A Z C / (GST) 3 c (MDA)! [J]. :;,2010,19(5):1014 1019. [12] VanderOostR,BeyerJ,VermeulenNPE.Fishbioaccu mulationandbiomarkersinenvironmentalriskassess ment:areview[j].environmentaltoxicolgyandphar macology,2003,13(2):57 149. [13] LiZ H,ZlabekV,GrabicR,etal.Efectsofexposureto sublethalpropiconazoleontheantioxidantdefensesys temand Na + K + ATPaseactivityinbrainofrainbow trout, 犗狀犮狅狉犺狔狀犮犺狌狊犿狔犽犻狊 [J].Aquat Toxicol,2010, 98:297 303. [14] LiZ H,ZlabekV,VelisekJ,etal.Modulationofgluta thione relatedantioxidantdefensesystemoffishchroni calytreatedbythefungicidepropiconazole[j].comp BiochemandPhysiol:PartC,2010,152:392 398. [15] LiZ H,VelisekJ,GrabicR,etal.Useofhematological andplasmabiochemicalparameterstoassessthechronic efectsofafungicidepropiconazoleonafreshwatertele ost[j].chemosphere,2011,83:572 578. [16] DingF,Song W H,GuoJ,etal.Oxidativestressand structure activityrelationshipinthezebrafish ( 犇犪狀犻狅狉犲狉犻狅 )underexposuretopaclobutrazol[j].jenvironsci Health:PartB,2009,44:44 50. [17] LiJ S,Sun L B,Zuo Z H,etal.Exposureto pa clobutrazoldisruptsspermatogenesisinmale 犛犲犫犪狊狋犻狊犮狌狊犿犪狉犿狅狉犪狋狌狊 [J].AquatToxicol,2012,122/123:120 124. [18],E,,].E D # Z 6-.! [J]. : ;,2009,29 (5):833 838. [19] DiGiulioR T,WashburnPC,WenningRJ,etal.Bio chemicalresponsesinaquaticanimals:areviewofdeter minantsofoxidativestress[j].environmentaltoxicolo gyandchemistry,1989,8(12):1103 1123. [20] HayesJ D,Pulford D J.Theglutathione S transferase supergenefamily:regulationofgstsandthecontribu tionoftheisoenzymestocancerchemoprotectionand drugresistance[j].criticalreviewsinbiochem istryand MolecularBiology,1995,30(6):445 600. [21] I,S,,]. (a)!n9 (D, Z 6 -.! [J].7, 2003,25(Sup.2):135 139. [22] LiJS,SunLB,ZuoZ H,etal.Efectsofpaclobutrazol exposureonantioxidantdefensesystem in 犛犲犫犪狊狋犻狊犮狌狊犿犪狉犿狅狉犪狋狌狊 [J].BulEnvironContam Toxicol,2012,89: 723 726. [23] Papadim IE,Loumbourdis N S.Exposureofthefrog Ranaridibundatocopperimpactontwobiomarkers,lip idperoxidation,and glutathione[j].buletin Environ mentcontaminanttoxicology,2002,69:885 891. [24] Zc, U,, V W,]. ( D 1 A Z 6-. 0 8! [J].= % :,2009,35(3):350 354. [25],E, 4. Na +,K + ATP GSH PX 5 0 MDA! [J].! %,2009,31(4):426 429. [26] B,,aS. D ( Na + /K + AT Pase5! [J]. Q : ;,2006,12 (2):220 223. [27] TanS H,YanF,LuoS A,etal.EfectsofNaNO2on Na + K + ATPaseand Mg 2+ ATPase activitiesin the liverof 犆犪狉犪狊犻狌狊犪狌狉犪狋狌狊 (Linnaeus)[J].ModernFish eriesinformation,2007,22(8):628. [28] =,X,. D * L ( 犇犪狀犻狅狉犲 狉犻狅 ) 6 5! [J]., 2009,4(5):700 704.
272 % (] ) 2013E [29] SanchoB,Ferrando M D,ANDREU.EInhibitionofgil Na + K + ATPaseactivityintheeel, 犃狀犵狌犻犾犪犪狀犵狌犻犾犪 byfenitronthion[j].ecotoxicologyand Environmental Safty,1997(38):132 1361. [30],L, W,]. D K ( 犆狔狀狅 犵犾狅狊狌狊狊犲犿犻犾犪犲狏犻狊 ) Z 6 6 Y% Na + K + ATPase 5! [J].7 #, 2009,40(3):367 372. [31] =, &!,X,].LAS Cd 2+ D Y Z SOD Na + K + ATPase5! [J]. %,2009,44(3):16 21. [32] I,,%,]. (a)!n9 ( D Z 6 5! [J].: ;, 2002,22(4):529 533. [33] P,M, c,]. D % " [J].. % :],2009,42(3): 76 80. 犈犳犳犲犮狋狊狅犳犘犪犮犾狅犫狌狋狉犪狕狅犾犈狓狆狅狊狌狉犲狅狀犃狀狋犻狅狓犻犱犪狀狋犛狔狊狋犲犿狅犳犉犻狊犺犛狆犾犲犲狀 LIJin shou 1,2,RUANJun feng 2,GENG Hong 1,WANGChong gang 1 (1.StateKeyLaboratoryofMarineEnvironmentalScience,XiamenUniversity,Xiamen361005,China; 2.DepartmentofBiology,NingdeNormalUniversity,Ningde352100,China) 犃犫狊狋狉犪犮狋 : Paclobutrazol (PBZ)iswidelyusedastriazolefungicideandplantgrowthregulatorinagriculture.Butthetoxicologyof PBZonfisheswasseldomstudied.ThisresearchwasdesignedtodetecttheinfluenceofPBZonthespleenantioxidantsystemof 犛犲 犫犪狊狋犻狊犮狌狊犿犪狉犿狅狉犪狋狌狊 thatwasexposedatanenvironmentalconcentrationofpbz (10,100,1000ng/L)for50d.Theresultshowed thattheglutathione (GSH),glutathione S transferases (GST),glutathioneperoxidase (GSHPx),malondialdehyde (MDA),and Na + K + ATPaseofspleenwerepresentedwithaconcentrationdependencevariationundertheactionofPBZ,whichindicatedtheex posureofpbzhadharmfultotheantioxidantsystemof 犛犲犫犪狊狋犻狊犮狌狊犿犪狉犿狅狉犪狋狌狊.Theresultshowedthatlong termusageofpbzmay causeecologicalpressureonaquaticorganismssuchasfishesandaquaticenvironmentetc.inviewofthelong termuseofpesticides thatmayleadtoenvironmentalproblems,itisnecessarytocarryouttheresearchesthatpesticideshavetoxicogenicefectonfishes andmarineorganisms.thisstudyhasactualsignificancefortheprotection,estimateandmonitoroftheoceanecologicalenvironment. 犓犲狔狑狅狉犱狊 : 犛犲犫犪狊狋犻狊犮狌狊犿犪狉犿狅狉犪狋狌狊 ;paclobutrazol;antioxidantsystem