16 6 2018 11 Chinese Journal of Bioprocess Engineering Vol. 16 No. 6 Nov. 2018 doi 10. 3969 /j. issn. 1672-3678. 2018. 06. 004 1 2 1 1 1. 330200 2. 330006 4 Box-Behnken 1 25 g /ml 121 92 min 2 6. 284% R284 A 1672-3678 2018 06-0019-05 Optimization of extraction of polysaccharides from Longya lily by high temperature pressure water extraction methodology LAN Haijun 1 HE Meijuan 2 LUO Chenxi 1 BI Shuangtong 1 1. Academician Workstation of Jiangxi Ganliang Industrial Co. Ltd. Nanchang 330200 China 2. Respiratory Medicine of Jiangxi Children's Hospital Nanchang 330006 China Abstract Using Lilium brownii var. viridulum from Jiangxi Wanzai as raw materials the effect of the ratio of material to liquid extraction temperature time and number of times on the yield of Lilium polysaccharides were studied and optimized by the Box - Behnken response surface methodology on the basis of single-factor experiment.the optimum extraction conditions were the material to liquid ratio was 1 25 g /ml extraction temperature 121 extraction time 92 minutes and extraction twice. Under these conditions the s from lilium was 6. 284%.This method is simple handy and more suitable for the extraction of Lilium. Keywords Lilium brownii var. viridulum Baker polysaccharide single - factor experiment response surface optimization Lilium brownii var. viridulum Baker 1 3 3 2 2017-07-13 2018-08-31 2017 2016 44 1981 E-mail 505716833@ qq.com. J 2018 16 6 19-23. LAN Haijun HE Meijuan LUO Chenxi et al.optimization of extraction of polysaccharides from Longya lily by high temperature pressure water extraction methodology J.Chin J Bioprocess Eng 2018 16 6 19-23.
20 16 4-7 1. 3. 2 5-9 10 H 2 SO 4 11 15 1. 0 ml 5 100 ml 1. 0 ml C 4. 0 ml - 30 min 490 nm 0 ~ 100 μg /ml 11 12 - Zhao 13 1 14 = VρN 100% 1 m V ml ρ g /ml N m g 1. 3. 3 1 120 1 h 2 g /ml 1 10 1 15 1 20 1 25 1 30 2 g /ml 1 20 1 h 2 100 110 120 130 135 1 1. 1 3 g /ml 1 20 120 2 30 60 90 120 150 min 1. 2 4 UV-2450 g /ml 1 20 120 BT224S 1 h 1 2 3 4 5 TD HH-2 1. 3. 4 RE52- A SN510C Design Expert 8. 05b Box-Behnken 1. 3 1. 3. 1 2 10. 0 g 250 ml 2. 1 4 000 r /min 1 1 15 min 50 mly = 0. 123 4+0. 003 75X R 2 = 0. 996
6 21 Fig. 1 1 The standard curve of glucose Fig. 3 2. 2. 3 2. 2 4 2. 2. 1 4 2 90 min 6. 25 ± 2 0. 03 % g /ml 1 20 6. 21±0. 04 % g /ml 1 20 17 16 g /ml 1 20 3 The effect of extraction temperature on the 4 Fig. 4 The effect of extraction time on the Fig. 2 2. 2. 4 2 5 The effect of material-liquid ratio on the 2. 2. 2 3 3 120 6. 20±0. 09 % 5 12 Fig. 5 The effect of extraction times on the 120
22 16 5 2 Box-Behnken 2 Table 2 Box-Behnken designs and results A B C /% 2 1-1 -1 0 5. 48 2 1-1 0 5. 79 2. 3 3-1 1 0 5. 56 2. 2 5-1 0-1 5. 32 2 6 1 0-1 5. 93 7-1 0 1 5. 67 8 1 0 1 6. 12 15 1 2 9 0-1 -1 5. 66 10 0 1-1 5. 84 1 11 0-1 1 5. 73 Table 1 Factors and levels of Box-Behnken test 12 0 1 1 5. 88 A / B / g ml -1-1 1 10 110 60 0 1 15 120 90 1 1 20 130 120 C / min Design Expert 8. 05b 2 0. 022 5AB-0. 04AC-0. 007 5BC-0. 034 7A 2-0. 329 5B 2-0. 267 8C 2 Y' = 6. 374+0. 221 25A+0. 072 5B+0. 081 25C+ 4 1 1 0 5. 96 13 0 0 0 6. 40 14 0 0 0 6. 31 15 0 0 0 6. 43 16 0 0 0 6. 35 17 0 0 0 6. 38 3 4 Table 3 3 Variance analysis and significance tests of regression model F P 1. 91 9 0. 21 31. 04 <0. 000 1 A 0. 39 1 0. 39 57. 42 0. 000 1 B 0. 042 1 0. 042 6. 17 0. 042 C 0. 053 1 0. 053 7. 74 0. 027 2 AB 0. 002 025 1 0. 002 025 0. 30 0. 602 8 BC 0. 006 4 1 0. 006 4 0. 94 0. 365 AC 0. 002 25 1 0. 002 25 0. 033 0. 861 A 2 0. 51 1 0. 51 74. 33 < 0. 000 1 B 2 0. 46 1 0. 46 67. 02 < 0. 000 1 C 2 0. 30 1 0. 30 44. 01 0. 000 3 4 Table 4 Variance analysis F P 0. 048 7 0. 006 821 0. 039 3 0. 013 6. 14 0. 056 0. 008 52 4 0. 002 13 1. 95 16 R 2 0. 975 6 R 2 adj 0. 944 1 3 P P<0. 000 1 R 2 = 0. 975 6 P
6 23 6 6 a 6 b 6 c 3 A C A B B C P Fig. 6 6 Response surface of the effects of the operating parameters on the 2. 4 6. 258% Design Expert 8. 05b g /ml 1 24. 98 121. 44 92. 25 min g /ml 1 25 121 92 min 6. 284% 3 10 g /ml 1 20 120 90 min 2 2 2014 12 J g /ml 1 25 121 92 min 6. 284% 0. 92% evaluation 1. -151. 2015 35 6 66-68. 2. M. antioxidant activities of polysaccharides from the leaves of Lilium 2002. J. 2015 36 12 167-171. 3. D. 17. 2007. J. 2018 36 2 58-62. 4. 5. J. 2017 8 8 3068-3073. 6. J. 2017 8 41-42. 7 JIN L ZHANG Y YAN L et al. Phenolic compounds and antioxidant activity of bulb extracts of six Lilium species native to China J.Molecules 2012 17 8 9361-9378. 8. D. 2011. 9 ZHANG X HAN X QIAN Z. Study on optimization of extraction process of saponins from Bulbus lilii and their antidepressant effects J.Asian J Chem 2014 26 18 5970-5972.. 2002 23 6 113-114. 11. 42 36 13047-13049... 2017 38 18 40-44. 13 ZHAO B T ZHANG J GUO X et al. Microwave - assisted extraction chemical characterization of polysaccharides from Lilium davidii var. unicolor Salisb and its antioxidant activities J.Food Hydrocolloids 2013 31 2 346-356. 14. J. 2014 40 8 263-267. 15.. 2016 23 7 146 16 XU Z WANG H D WANG B L et al. Characterization and lancifolium Thunb J.Int J Biol Macromol 2016 92 148-155.