CloneEZ ONEHOUR Western ELISA ELISA Protein A ELISA MarkerPCR estain 2.0 e THE

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www.genscript.com.cn CloneEZ ONEHOUR Western ELISA ELISA Protein A ELISA MarkerPCR estain 2.0 e THE

PCR CloneEZ PCRPCR PCRPCR Taq DNA polymerasegreen DNA Taq polymerase (E00043) 3714 DNA 带有与线性化载体末端有 1520 个相同碱基的 PCR 引物 目的基因 PCR 产物 线性化载体 DNAGreen Taq Green Stabilized Taq DNA polymerase Taq DNA polymerase 传统 PCR 产物克隆与 CloneEZ 重组试剂盒克隆对比 线性化载体 单管反应 30 分钟 PCR 重组载体转化CloneEZ 重组克隆试剂盒原理 Green Taq DNA Polymerase 100 μl 10 Reaction Buffer 1.5 ml Stabilized dntp Mix 300 μl Units of Taq (U) 1.0 1.5 1.5 2.0 2.0 2.5 2.5 3.0 3.0 2.5 2.5 8 kbλdnataq Green Taq ( Green Taq Green Taq3714 1 ( ) CloneEZ 1 kit (24 rxns) L00339 1,750 ( ) PCR 1 kit L00342 640 1 2

0.005 EU/ml EU/ml ToxinSensor LAL 545 nm ToxinSensor Endotoxin Concentration (EU/ml) 1.2 1 0.8 0.6 0.4 0.2 0 0.2 0 X y = 0.3271x 0.1344 R 2 = 0.8132 Endotoxin Concentration (EU/ml) 0 0.2 0.4 0.6 0.2 0 0.2 0.4 0.6 OD (545 nm) OD (545nm) 1.2 1 0.8 0.6 0.4 0.2 y = 2.1386x 0.0107 R 2 = 0.9986 LAL ToxinSensor 1 kit ( ) 1 mg/mlph 7.4NiNTA ToxinSensor 1 kit ( ) 1: 200,0001: 400,0001: 800,000LAL ToxinSensor 1 kit (40) Positive control + Negative control 1:200,000 50,000100,000 EU/ml + 1:400,000 1:800,000 L00350C L00350 L00351 ( ) 880 1,650 990 3 4

E 配置齐全, 操作方便 高稳定性, 高去除效率 高结合力 :> 2,000,000 EU/ml (CV) ONEHOUR Western ( ) ONEHOUR Western ONEHOUR Pretreat Western A&B Solution (We ToxinEraser 含内毒素污染的溶液 B (PMB) ToxinEraser 试剂盒内容 (L00338) ToxinEraser Endotoxin Removal Resin (M01053) 35 次实验使用 1.5 ml 125 ml 内毒素结合到去除树脂上 过滤内毒素后的溶液流出 获得去内毒素的溶液, M01013) (M01054) 125 ml 1 ( ) 1 (3) ONEHOUR Western Basic (Rabbit) 1 kit (5 Assays) L00204 600 (1 ml) 2 (6) ONEHOUR Western Basic (Mouse) 1 kit (5 Assays) L00205 600 ONEHOUR Western Standard (Rabbit) 1 kit (5 Assays) L00204C1 750 1 ml (2 ml of 50% slurry) 1 kit L00402 L00338 ( ) 520 1,210 ONEHOUR Western Standard (Mouse) Western QuickBlock : Pretreat A (50 ml) & B (50 ml) Solution 1 kit (5 Assays) 1 pk L00205C1 M01013 750 300 125 ml M01053 250 125 ml M01054 250 5 6

S ELISA GST GSTGSTBiotinGST HRPStreptavidinBB HRP 450 nm (OD) 1 ng/ml GST 3.125~100 ng/ml GST ( ) His Tag ELISA Detection Kit 1 kit L00436 2,980 ( ) His Mini Purification and Detection Kit His Tag Antibody Plate (black, 96 wells, nonremovable) 1 kit 5 plates L00438 L00440b 4,490 1,790 S (GST tag) ELISA 1Kit (96 assay (GST tag ELISA Detection Kit) L00411 2,980 His Tag Antibody Plate (clear, 8 12 strips) His Tag Antibody Plate (white, 96 wells, nonremovable) 5 plates 5 plates L00440c L00440w 1,790 1,790 LanPower 6 His Tag Detection Kit 1000.0 tests L006531000 9,500 7 8

Marker bp 1,500 (25 ng/μl) 1,000 (25 ng/μl) 800 (25 ng/μl) 600 (25 ng/μl) 500 (50 ng/μl) DNA Marker: 即用型 100 bp DNA Ladder (M102R) 400 (25 ng/μl) 2.0% agarose gel 300 (25 ng/μl) 浓度 :500 ng/5 μl; 保存缓冲液 :10 mm 200 (25 ng/μl) TrisHCl (ph 7.5)1 mm EDTA 金斯瑞即用型 (readytouse ) 100 bp DNA ladder 产品可以在已结合染料 ( 例如 EB) 的琼 100 (25 ng/μl) 脂糖胶上直接上样 ; 若没有结合 Loading Buffer, 需要结合 Loading Buffer 后上样 bp 2,000 (25 ng/μl) 1,500 (25 ng/μl) 1,000 (25 ng/μl) 700 (25 ng/μl) 500 (50 ng/μl) 300 (25 ng/μl) 100 (25 ng/μl) DNA Marker: PCR DNA Ladder (M106R) 2.0% agarose gel 浓度 :400 ng/5 μl, 保存缓冲液 :10 mm TrisHCl (ph 7.5)1 mm EDTA 金斯瑞即用型 (readytouse ) PCR DNA ladder 可以在已结合染料 ( 例如 EB) 的琼脂糖胶上直接上样 ; 若没有结合 Loading Buffer, 需要结合 Loading Buffer 后上样 Protein A20 pg/mlprotein Protein A SuRe AMabSelect SuRe 50 pg/ml Protein AProtein AProtein A Protein A 80 kd 30 kd 20 kd 10 kd Protein Marker: PAGEMASTER Protein Standard (M00516) 金斯瑞蛋白 Marker: PAGEMASTER Protein Standard 是由 7 种高纯度的重组蛋白混合而成, 专为 SDS 电泳设计, 分子量范围为 10 ~ DNA Marker: 100bp DNA Ladderreadytouse DNA Marker: PCR DNA Ladder ReadytoUse Plus 100 bp DNA Ladder Protein Marker: PAGEMASTER Protein Standard Protein Marker for Fluorescent Western Blotting WBMASTER Protein Standard PAGEMASTER Protein Standard Plus 500 μl 500 μl 500 μl 500 μl 250 μl 500 μl 500 μl 80 kd 50 kd 30 kd 20 kd Protein Marker: WBMASTER Protein Standard (M00521) WBMASTER Protein Standard 用于 western blot, 由 7 种高纯度的重组蛋白混合而成, 分子量范围在 20 ~120 KDa 之间 在同一次 western blot 实验中, 无需额外试剂, 可在同一张转印膜上直接观察到样品和该 marker 的条带 M102R100 M106R100 M105R500 M00516 M00124 M00521 MM1397500 ( ) 150 100 150 200 585 320 240 Protein A MabSelect SuRe Protein A intraassay # of tests Meaning/ml CV% 12 12 12 1.60 0.40 0.10 1.60 4.05 4.56 LOD LOQ 20 pg/ml 50 pg/ml 50 pg/ml 100 pg/ml interassay # of tests Meaning/ml CV% 8 1.60 3.70 8 0.40 5.42 8 0.10 7.59 CV<5% & CV<10% ( ) Taq DNA Polymerase Taq DNA Polymerase Green Taq DNA Polymerase 2x Taq Master Mix dntp mixture, 10 mm each 50000 U (50 1000 U) 1,000 U 1,000 U 100 rxns 0.5 ml E0000750000 E000071000 E00043 E00019 D0056 ( ) 120 600 160 80 Protein A ELISA 1 kit L00430 5,500 9 10

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 estain2.0 1, 615 μl NEB (P7703S): 10 KDa15 KDa20 KDa25 KDa 30 KDa40 KDa50 KDa60 KDa80 KDa100 KDa150 KDa250 KDa; 2, 5, 14 5 μl 3, 8, 513 μl ) ( 4, 6, 7, 512 μl 10 (MM1397500): KDa15 KDa20 KDa 30 KDa40 KDa50 KDa60 KDa80 KDa120 KDa15 KDa 9, 10, 11 200 ng400 ng800 ng ExpressPlus PAGE Gel, 412%, 10 wells ExpressPlus PAGE Gel, 8%, 12 wells ExpressPlus PAGE Gel, 10%, 12 wells ExpressPlus PAGE Gel, 12%, 12 wells 80 μl ExpressPlus PAGE Gel, 8%, 10 wells ExpressPlus PAGE Gel, 10%, 10 wells ExpressPlus PAGE Gel, 12%, 10 wells ExpressPlus PAGE Gel, 420%, 10 wells ExpressPlus PAGE Gel, 816%, 10 wells ExpressPlus PAGE Gel, 420%, 12 wells ExpressPlus PAGE Gel, 816%, 12 wells ExpressPlus PAGE Gel, 412%, 12 wells ExpressPlus PAGE Gel, 8%, 15 wells ExpressPlus PAGE Gel, 10%, 15 wells ExpressPlus PAGE Gel, 12%, 15 wells ExpressPlus PAGE Gel, 420%, 15 wells ExpressPlus PAGE Gel, 816%, 15 wells ExpressPlus PAGE Gel, 412%, 15 wells M00810 246 M01010 246 M01210 246 M42010 459 M81610 459 M41210 459 M00812 246 M01012 246 M01212 246 M42012 459 M81612 459 M41212 459 M00815 246 M01115 246 M01215 246 M42015 459 M81615 459 M41215 459 BioRad MiniPROTEAN II &3 BioRad MiniPROTEAN Tetra System LONZA PAGEr Minigel Chamber Hoefer Mighty Small (SE 260/ SE 250) Hoefer Tall Mighty Small (SE 280) Invitrogen Novex XCell I, II & Surelock (Invitroge 1 Box (5/PK) M00138 250 500 ml M00519500 450 1 Box (10/PK) 5 ml M00139 MB01015 580 240 11 12

eblot estain 2.0 快速 :7 分钟即可完成染色实验简便 : 触键即可操作 洁净 : 无溶液及缓冲液需求安全 : 唯一明确表示不使用甲醇的染色法 eblot (Western eblot Blot) eblot eblot eblot eblot 快速高效, 可在 710 分钟内完成转印过程 操作简便, 人性化的流程设计方便实验人员操作, 减少人为操作失误 不含甲醇等有害成分 estain 2.0 适用性强, 可用于不同类型的聚丙烯酰胺凝胶 检测灵敏度高, 优于传统湿法转印 A 1 2 3 4 5 6 7 8 9 10 estain 2.0 padstart7 estain 2.0 eblot Start7 eblot eblot ( ) ( ) estain 2.0 estain (R250, 20pak) estain (R250, 2 20pak) estain (G250, 20pak) estain (G250, 2 20pak) (100 mm, 1pak) 1 unit 1 box 2 boxes 1 box 2 boxes 1 unit L02016 L02011 L020112 L02012 L020122 L02017 7,990 590 940 590 940 590 eblot eblot (Basic, 20pak) eblot (Nitrocellulose, 20pak) eblot (PVDF, 20pak) eblot eblot 1 unit 1 box (20pak) 1 box (20pak) 1 box (20pak) 1 unit 125 ml L03010 L03011 L03013 L03014 L03012 M01078 8,990 790 1,590 1,090 590 590 13 14

( ) NiNTA Resin NiNTA Resin (with 5 columns) 10 ml20 ml of 50% Slurry 10 ml20 ml of 50% Slurry L00250 L00250C 1,300 1,420 L00250CL00250 NiIDA (L00223I/L00223C) NiNTA(L00250/L00250C) 10 ml 20 mg 6 His (27 kd)/ml 90 μm (45165 μm) 1 PBS (20% 4% 2818 NiIDA Resin 10 ml20 ml of 50% Slurry L00223I NiIDA Resin (with 5 columns) 10 ml20 ml of 50% Slurry L00223C L00223CL00223I ( ) 1,200 1,350 ( GST GST(L00206)S ( 1 2 3 4 5 6 7 8 9 (50%)GST 75 /ml GST(L00207)L00206 0.154 g 50 E.coli30 kdninta Resin Lane1: (X) Lane5: () 35 Lane2: (X) Lane6: () 25 Lane3: (X) Lane7: () Lane4: (X) Lane8: () GST TabNiNTA 20mg GST/ml 3 6 M Gu HCl 8 M Urea _ 2% Triton X100 2% Tween 20 1% CHAPS 20 mm βme 1 mm DTT _ 4 M MgCl 2 5 mm CaCl 2 2 M NaCl 50% glycerol 20% ethanol 1 mm EDTA HighAffinity GST Resin GST Fusion Protein Purification 10 ml (20 ml of 50% slurry) 1 kit L00206 L00207 ( ) 980 1,390 15 16

Protein A Protein A (L00210) Protein A IgG IgG Protein AIgG Fab IgG Protein G Ultra Protein A ( L00403) Ultra Protein AL00400Protein Protein AL00210) A 1 mg 43040 mg higg/ml ( L00404) X 200 M 1 2 3 4 150 100 AntiHistag IgG 75 X AntiHistag SDSPAGE IgG Histag 50 Serum albumin X 35 M: Smart BroadRange Protein Standard ( MM0906) 25 ( ) Lane 1: () Lane 2: ) ( 15 Protein A 5 ml (10 ml of 50% slurry) L00210 980 Lane 3: (X) Lane 4: (X) Ultra Protein A 5 ml (10 ml of 50% slurry) L00400 2,490 ( ) HighAffinity Iodoacetyl Resin 10 ml (20 ml of 50% slurry) L00403 1,500 HighAffinity Antibody Purification 1 kit L00404 1,390 HighAffinity Iodoacetyl Resin Empty Column 5 Coupling Buffer Block Buffer (Solid) Storage Buffer 5 Binding/Wash Buffer Elution Buffer Neutralizing Buffer 5 ml (10 ml of 50% slurry) 3 30 ml 10 ml (10 ml of 1 Coupling Buffer to the solid) 20 ml 70 ml 70 ml 15 ml 17 18

60 45.00 Dynamic binding capacity (mg/ml resin) 50 40 30 20 Monofinity A Resin0.1M NaOH Monofinity A Resin0.5M NaOH 10 Mabselect SuRe0.1M NaOH Mabselect SuRe0.5M NaOH 0 0 50 100 150 200 250 Cycle No. Leakage (ng/mg of IgG) 30.00 15.00 0.00 1 20 40 60 80 100 150 200 Cycle No. Protein A Protein AMonofinity A ResinL00433 Protein AFc Protein A protein A 50300 cm/h ( ) 4% 在各种用于 protein A 亲和层析的水相缓冲液中均保持稳定 Protein A(1 1 ml ml 1) L0043311 1,690 90 μm 312 Protein A(1 1 ml 5 ml 5) L0043315 5,490 50 mgigg/ml 0.10.5 N NaOH Protein A(5 5 ml ml 1) L0043351 4,990 (RT=5=300 cm/h) 20 % 乙醇,28 Protein A(5 5 ml 5 ml 5) L0043355 18,090 Protein A 25 ml L0043325 16,800 Protein A 200 ml L00433200 33,500 Protein A 1 L L004331 Protein A 5 L L004335 Protein A 10 L L0043310 Protein A L00433c 19 20

THE His Tag ( ) Protein G Resin 5 ml (10 ml of 50% slurry) L00209 1,290 Protein L Resin 2.5 ml (5 ml of 50% slurry) L00239 1,450 Streptavidin Resin 5 ml (10 ml of 50% slurry) L00353 1,390 () ( ) ( ( ) ( ) THE His tag (Clone 6G2A9 ) ng6 His 5 His4 H Histag Protein A 4 ml L00273 890 Table1: THE His tag Antibody Protein G Protein A/G NiCharged Glutathione Streptavidin Mouse AntiHis mab Mouse AntiGST mab AntiDYKDDDDK G1 mab 2 ml 2 ml His 8 ml GST 8 ml 2 ml His 2 ml GST 2 ml DYKDDDDK 1 ml L00274 L00277 L00295 L00327 L00424 L00275 L00336 L004461 1,250 1,480 980 1,080 1,650 1,970 1,590 2,990 THE His Tag Antibody, mab, Mouse (A00186) THE His Tag Antibody [HRP], mab, Mouse (A00612) THE His Tag Antibody [Biotin], mab, Mouse (A00613) THE His Tag Antibody [FITC], mab, Mouse (A01620) ELISA 0.050.2 μg/ml 0.050.5 μg/ml 0.020.2 μg/ml Western blot 0.10.2 μg/ml 0.11.0 μg/ml 0.51.0 μg/ml Immunoprecipitation (IP) 1 μg/ml Immunofluorescent staining (IF) 1 μg/ml 0.12 μg/ml Flow cytometry 1 μg/ml 0.12 μg for 1 x 10 6 cells 21 22

1. THE His Tag A B C 1 2 3 4 X 1 2 3 4 Histag ( M0101) C HisCHO CHO HisCHO 3:THE His Tag A:His Tag HEK293 B:His CHis TagCHO 60 30 15 7.5 3. THE His Tag Counts 10 20 30 40 50 60 70 0 10 0 10 1 10 2 10 3 10 4 A B 1A (A, A00186, 1 μg/ml) X (B, 34670, 1 μg/ml) His tagb:xthe His Tag (A00186) THE Histag mab 2. A 1 2 3 B 1 2 3 (ng) C 1 2 3 100 50 NC 100 50 NC 100 50 NC M 115 kd 75 kd 50 kd 32 kd 25 kd 15 kd (ng) (ng) 25.0 12.5 6.25 3.13 25.0 12.5 6.25 3.13 M 1 2 3 4 100 10 1 100 10 1 100 10 1 100 10 1 (ng) Histag THE Histag[HRP] mab THE Histag [Biotin] mab THE Histag [FITC] mab THE Histag [ifluor 488] mab THE Histag [ifluor 555] mab THE Histag [ifluor 647] mab : Immunoprecipitation (IP), western blot (WB), immunochemistry/immunofluorescence (ICC/IF), flow cytometry (FACS). Multiple Tag (Purified) Multiple Tag Cell Lysate NiIDA Resin NiNTA Resin 21:THE His Tag (A00186, 1 μg/ml) 22 μ) ( C (A)N (B) His (C) Nicharged MagBeads HexaHis HexaHis Control Control Purification Purification Purification, IP Unconjugated HRP Biotin FITC ifluor 488 ifluor 555 ifluor 647 ELISA, WB, IP, IF, FACS ELISA, WB ELISA, WB FACS, IF FACS, IF FACS, IF FACS, IF Immunogen, Antibody affinity purification Immunogen, Antibody affinity purification 200 μg 200 μg 10 ml (20 ml of 50% slurry) 10 ml (20 ml of 50% slurry) 8 ml 1 mg 5 mg ( ) A00186 A00612 A00613 A01620 A01800100 A01801100 A01802100 3,750 4,100 4,100 4,100 4,500 4,500 4,500 ( ) M0101 980 M0100 720 L00223I 1,200 L00250 1,300 L00295 980 RP117371 710 RP117375 1,900 A. FITCHis His (THE Antibody[FITC], A01620) HEK293 IgG (A01007) His (A00186) B. THE His Antibody, A00186 1His 2IgG (A01007) 3His (A00186) 23 24

THE GFP THE cmyctag Antibody, mab, Mouse THE GSTtag Antibody, mab, Mouse THE HAtag Antibody [Biotin], mab, Mouse THE HAtag Antibody, mab, Mouse THE HAtag Antibody [HRP], mab, Mouse THE DYKDDDDK Tag Antibody, mab, Mouse THE GFP Antibody, pab, Rabbit THE V5 Tag Antibody, mab, Mouse THE NWSHPQFEK (Strep II) Tag Antibody THE cgmp Antibody, mab, Mouse THE V5 Tag Antibody [ifluor 488], mab, Mouse THE HA Tag Antibody [ifluor 488], mab, Mouse THE DYKDDDDK Tag Antibody [ifluor 488], mab, Mouse THE Phosphotyrosine Antibody (E10) plus ( ) A00704 1,750 A00865 1,750 A01297 2,990 A01244 2,870 A01296 2,990 A00187 1,390 A01704 4,120 A01724 2,190 A01732 3,190 A01508100 2,870 A01803100 2,630 A01806100 3,440 A01809100 1,670 A01819 2,650 (GFP)27 kd (395 GFP GFP () A00185 A01704 A01388 A01694 A01703 Mouse Rabbit Rabbit Chicken Goat ELISA, WB IP, WB, ICC/IF, FACS ELISA, IP, WB IP, WB IP, WB mab, IgG2b IgG IgG IgY IgG : Immunoprecipitation (IP), western blot (WB), immunochemistry/immunofluorescence (ICC/IF), flow cytometry (FACS). Loading Antibody dilution 1: 1,000 cmyc mab popular clone 9E10 1: 3,000 1: 9,000 1: 27,000 1: 81,000 1: 243,000 1: 729,000 A00704 (2G8D5) cmyc (5 ng) 11THE cmyc mab9e10western 9E1027 A00187 X M2 1 2 3 1 2 3 100 50 25 100 50 10 50 10 5 100 50 25 100 50 10 50 10 5 (ng) 1: 1,000 1: 3,000 1: 9,000 1: 27,000 1: 81,000 1: 243,000 1: 729,000 GFPRFP GFP Antibodies A01704 A01388 A01694 A01703 A00185 GFPRFP GFP/GFPfusion proteins DsRed DsRed2 AcGFP1 mcherry egfp tdtomato morange GFP Variants Specificity egfp AcGFP1 tdtomato RFP Variants egfp AcGFP1 cgfp CFP YFP GFPuv OFP DsRed morange mcherry tdtomato DsRed2 DsRed morange DsRed2 mcherry Nterminal Internal Cterminal Nterminal Internal Cterminal 12 THE DYKDDDDK(A00187) X AntiFlag M2 13Western blot 14EGFA431 1. Phosphotyrosine Antibody (5E10), mab, Mouse (A01817, 1 µg/ml); 2. Phosphotyrosine Antibody (18E10), mab, Mouse (A01818, 1 µg/ml); FlagTHE 3. Phosphotyrosine Antibody (E10) plus (A01819, 1 µg/ml); DYKDDDDK X AntiFlag M2 4. AntiPhosphotyrosine, 4G10 Platinum (Millipore, 051050, 1:1000); GFPRFP GFP (1 μg/mlrabbita01704) GFPRFP GFP (1 μg/mlgoata01703) 25 26

egfp tdtomato morange DsRed DsRed2 13:GFPRFP GFP14GFPRFP GFP (1 μg/mlchickena01694) AcGFP1 mcherry egfp AcGFP1 cgfp DsRed DsRed2 mcherry (1 μg/mlmousea00185) tdtomato 50 10 5 2 1 (ng) GFP 23GFP(0.2 μg/mlgoata01703) 50 10 5 2 1 (ng) GFP 24GFP(1 μg/mlchicken A01694) 1 2 3 4 1 2 3 4 egfp tdtomato AcGFP1 DsRed DsRed2 morange mcherry egfp tdtomato AcGFP1 DsRed DsRed2 morange mcherry X 15:GFP(1 μg/ml,mousea00185) XGFP (1 μg/mlmouse332600) 1OFP293 2EGFP293 3GFPuv 4293 200 100 50 10 (ng) GFP X, ab290, A01704 25GFP(1 μg/mlrabbita01388) 26THE (0.2 GFP μg/mlrabbita01704) XGFP (1:5,000rabbitab290) GFP (21, 22) GFP GFP(chicken,A01694goat,A01703) GFPGFP 1 2 3 1 2 3 上样 50 10 5 2 1 50 10 5 2 1, A01704 X, ab290 21 THE GFP(0.2 μg/mlrabbita01704) 22 GFP(1 μg/ml,mousea00185) GFP GFP(1:5000rabbitab290) GFP 100 25 10 NC 100 25 10 NC (ng), A00185 X, 332600 (1 μg/mlmouse332600) GFP IgG GFP 31:THE GFP(rabbitA01704) 1GFP 2IgG(A01008) 3THE (rabbita01704) GFP IgG GFP 32:GFP(goatA01703) 1GFP 2IgG(A01009) 3GFP(goatA01703) 27 28

1 2 3 1 2 3 IgG GFP 33:GFP(rabbitA01388) 1GFP 2IgG(A01008) 3GFP(rabbitA01388) GFP 34:GFP(chicken A01694) 1GFP 2IgY(A01010) 3GFP(chicken A01694) 35THE GFP(rabbitA01704) 36GFPHEK293 HEK293 GFPHEK293 AIgG(A01008) A BTHE GFP(rabbitA01704) B Avitag Antibody, pab, Rabbit CBPtag Antibody, pab, Rabbit cmyctag Antibody, pab, Rabbit DDDDKtag Antibody, pab, Rabbit DYKDDDDKtag Antibody, pab, Rabbit E2tag Antibody, pab, Rabbit GSTtag Antibody, pab, Rabbit GSTtag Antibody [Biotin], pab, Rabbit GSTtag Antibody [HRP], pab, Rabbit HAtag Antibody, pab, Goat HAtag Antibody [Biotin], pab, Goat HAtag Antibody [HRP], pab, Goat HATtag Antibody, pab, Rabbit KT3tag Antibody, pab, Rabbit MBPtag Antibody, mab,mouse NWSHPQFEK Strep II pab, Rabbit Protein A Antibody, pab, Chicken RFPtag Antibody, pab, Rabbit S1tag Antibody, pab, Rabbit SNAPtag Antibody, pab, Rabbit Stag Antibody, pab, Rabbit Tag100tag Antibody, pab, Rabbit TAPtag Antibody, pab, Rabbit Trxtag Antibody, mab, Mouse Universaltag Antibody, pab, Rabbit V5tag Antibody, pab, Rabbit VSVGtag Antibody, pab, Rabbit VSVGtag Antibody [HRP], pab, Rabbit A00674100 A00635100 A00172100 A00676100 A00170100 A00630100 A00097100 A00202100 A00130100 A00168100 A00203100 A00169100 A00636100 A00632100 A00190100 A00626100 A00728100 A00682100 A00679100 A00684100 A00625100 A00677100 A00683100 A00180100 A00680100 A00623100 A00199100 A00872100 ( ) 2,180 2,180 2,180 2,180 2,180 GFPtag Antibody, mab, Mouse GFP Antibody, pab, Rabbit GFP Antibody, pab, Chicken GFP Antibody, pab, Goat THE GFP Antibody, pab, Rabbit ELISA, WB IP, WB, ICC/IF, ELISA ELISA, IP, WB ELISA, IP, WB IP, WB, ICC/IF, ELISA, FACS ( ) A00185 A01388 A01694 A01703 A01704 4,120 ( ) LanPower 6 His Tag Antibody [Eu] LanPower GST Tag Antibody [Eu] LanPower FLAG Tag Antibody [Eu] LanPower 6 His Tag Antibody [GS665] LanPower GST Tag Antibody [GS665] LanPower 6 His Tag Detection Kit LanPower GST Tag Detection Kit 10 μg 10 μg 10 μg 1000 assays 1000 assays A0181210 A0181310 A0181410 A01815100 A01816100 L006531000 L006541000 2,000 2,340 2,340 6,750 6,750 9,500 9,500 29 30

产品名称规格产品编号价格 kd 120 85 60 40 22 M 293 cell lysate (μg) 20 4 A 0.8 20 4 0.8 B A. 0.1 μg/ml Mouse Antiβactin mab (A000702) B. 0.1 μg/ml Mouse Antiβactin mab () Goat AntiMouse IgG (H&L) [HRP] pab (1:10000 A00160) Lumisensor HRP SubstrateL00221V500 Antiβactin Chicken IgY Control (Whole Molecule), Purified Goat IgG Control (Whole Molecule), Purified Human IgG Control [FITC] (Whole Molecule), Purified Human IgG Control (Whole Molecule), Purified Mouse IgG control (Whole Molecule), Purified Mouse IgG Control [FITC] (Whole Molecule), Purified Rabbit IgG Control (Whole Molecule), Purified Mouse IgG2b control (Whole Molecule), Purified Rabbit IgG Control [FITC] (Whole Molecule), Purified 4.0 mg 4.0 mg 0.5 mg 4.0 mg 1.0 mg 0.5 mg 4.0 mg 1.0 mg 0.5 mg A01010 A01009 A01383 A01006 A01007 A01381 A01008 A01730 A01382 690 690 690 690 690 690 690 690 690 产品名称 规格 产品编号 价格 其他规格 THE βactin Antibody, mab, Mouse GAPDH Antibody, pab, Goat THE αtublin Antibody, mab, Mouse THE αtublin Antibody [HRP], mab, Mouse A00702 A00191 A01410 A01490 1,750 2,250 3,350 产品名称 规格 产品编号 价格 Goat AntiChicken IgY Antibody (H&L) [HRP], pab Goat AntiRabbit IgG Antibody (H&L) [HRP], pab Goat AntiRat IgG Antibody (H&L) [HRP], pab Donkey AntiGoat IgG Antibody (H&L) [HRP], pab Goat AntiMouse IgG Antibody (H&L) [HRP], pab Goat AntiRabbit IgG Antibody (H&L), pab Goat AntiRabbit Human Antibody (H& L) [HRP], pab Goat AntiMouse Human Antibody (H&L) [FITC], pab 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg A00165 A00098 A00167 A00178 A00160 A00131 A00166 A01318 790 790 790 790 790 500 790 790 31 32

0.2 Relative Fluorescence Unit(RFU) 1 BMP2 (GenScript_1st Batch) BMP2 (GenScript_2nd Batch) 0.8 BMP2 (Supplier A) 0.6 0.4 0 3 2 1 0 1 2 hbmp2 (μg/ml) Cell Proliferation Assay for Human BMP2 (Cat.No.Z02913) Using C2C12 Cells OD (490 nm) 1.60 Human VEGF (CelKine 1st batch) 1.40 Human VEGF (CelKine 2nd batch) 1.20 Human VEGF (Supplier A) 1.00 0.80 0.60 0.40 0.20 0.00 0.00 0.01 0.10 1.00 10.00 Cell Proliferation Assay for Human VEGF (Cat.No.Z02689) Using HUVEC Cells 0.2 EU/μg1 EU/μg hvegf (ng/ml) [log scale] ( ) OD (490 nm) 1.40 1.20 1.00 0.80 0.60 0.40 0.20 0.00 0.20 0.001 0.01 0.1 1 10 hifnγ (ng/ml) Cytotoxicity Assay for hifn gamma (Cat.No.Z02915) Using HT29 (HTB38) Cells Bone Morphogenetic Protein2 (BMP2), Human Stem Cell Factor (SCF), Human TNFalpha (mutant), Human GCSF, Human Interleukin15 (IL15), Human Interleukin4 (IL4), Human E.coli P.pastoris E.coli E.coli E.coli CHO > 95% > 95% > 95% > 95% > 96% > 95% < 1.0 EU/μg < 1.0 EU/μg < 0.1 EU/μg < 0.2 EU/μg < 1.0 EU/μg < 0.2 EU/μg Z02913 Z02692 Z00404 Z02974 Z00377 Z02925 OD (490 nm) 1.20 1.00 0.80 0.60 0.40 0.20 0.00 0.20 0.00 0.01 0.10 1.00 10.00 mvegf (ng/ml) Cell Proliferation Assay for Mouse VEGF (Cat.No.Z02690) Using HUVEC Cells Epidermal Growth Factor (EGF), Human E.coli > 95% < 1.0 EU/μg Z00333 Interleukin2 (IL2), Human CHO > 95% < 1.0 EU/μg Z03074 Enterokinase, Light Chain, Porcine Yeast / / Z01003 33 34

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