518 9 content and GST activities of livers of C. cathayensis showed no significant difference between HH1 and MH63 rice habitat. The activities of ACP

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1 Asian Journal of Ecotoxicology Vol. 9, 2014 No.3, DOI: /AJE cry1ab/ac 2014, 9(3): Tan S H, Liu Y F, Sun Y D, et al. The response of antioxidant system and metabolic enzyme of Cipangopaludina cathayensis to transgenic Bt gene rice [J]. Asian Journal of Ecotoxicology, 2014, 9(3): (in Chinese) - cry1ab /Ac 谭树华 1, 刘雨芳 1,*, 孙远东 1, 肖璐 1, 李菲 1, 桂芳艳 1, 刘文海 1 2, 戈峰 Bt 1 (HH1) Bt 63(MH63) 63(MH63C)3 (SOD CAT GSH-PX GSH) (GST ACP) HH1 SOD CAT GSH-PX MH63 SOD CAT (p < 0.01) GSH GST HH1 MH63 HH1 ACP MH63 (p < 0.01) MH63C MH63 MH63C SOD CAT GST GSH-PX GST GSH MH63C Bt X171.5 A The Response of Antioxidant System and Metabolic enzyme of Cipangopaludina cathayensis to Transgenic Bt Gene Rice Tan Shuhua 1, Liu Yufang 1,*, Sun Yuandong 1, Xiao Lu 1, Li Fei 1, Gui Fangyan 1, Liu Wenhai 1, Ge Feng 2 1. School of Life Sciences, The Key Laboratory of Horticultural Crops Pest Management in Hunan Province, Huan University of Science and Technology, Xiangtan , China 2. Institute of Zoology, Chinese Academy of Science, Beijng , China Received 20 January 2014 accepted 26 February 2014 Abstract The activities of antioxidative (SOD, CAT, GSH-Px, GSH) and metabolic enzymes (GST, ACP) of Cipangopaludina cathayensis that grown in three kinds of rice habitat including insect-resistant transgenic Bt rice Huahui No 1 HH1, non-bt conventional rice Minghui63 " (MH63 ) without pesticides, and non-bt rice (MH63C) with pesticide were detected in this study. The results showed that the activities of SOD, CAT, GSH- Px of livers and gills of C. cathayensis grown in HH1 rice habitat were all higher than that in MH63 habitat. The difference of SOD and CAT activities between HH1 and MH63 habitat were significant (p < 0.01), while GSH (2012ZX ) ( 1972-) [email protected] * (Corresponding author) [email protected]

Asian Journal of Ecotoxicology, 2014, 9(3): 517-523 (in Chinese) - cry1ab /Ac 谭树华 1, 刘雨芳 1,*, 孙远东 1, 肖璐 1, 李菲 1, 桂芳艳 1, 刘文海 1 2, 戈峰 1. 411201 2.

2 518 9 content and GST activities of livers of C. cathayensis showed no significant difference between HH1 and MH63 rice habitat. The activities of ACP in HH1 rice habitat were higher than in MH63 rice habitat significantly (p < 0.01). As for MH63 and MH63C habitat, the activities of SOD, CAT and GST of livers in MH63C habitat increased significantly, meanwhile GSH-Px GST activities and GSH content of gills in MH63C habitat were activated significantly. It suggested that the antioxidative enzymes and detoxification metabolic enzymes of C. cathayensis under pesticides were activated significantly, while antioxidative enzymes and metabolic enzymes were induced in HH1 transgenic Bt rice habitat. It could be concluded that the toxic effect of Bt rice on C. cathayensis was apparently lower than that of the pesticides. Keywords Cipangopaludina cathayensis; biotech rice; antioxidative enzyme activities; detoxification metabolic enzyme enzymes -, (Bacillus thringiensis (Bt 1 Materials and methods ) Bt Bt [1] Bt Bt cry1ab/ac 1 [2-5] [6] (HH1) 63(MH63) [7,8] cry1ab/cry1ac [9,10] Bt 1 (HH1) ( (2009) 072 ) HH m 2, m 2, [11] (Folsomia candida) 3 3 (3 ) 1 [12] 1 (HH1) [13] Bt (MH63) 1 (MH63C) (Cipangopaludina cathayensis) MH63C % 35% 27% 5 s 25% % 25% 30% 25%,, [14,15], [16] [17] 2.5 g CAT [18] 3 ( = 50 cm 100 cm) 8 m

As for MH63 and MH63C habitat, the activities of SOD, CAT and GST of livers in MH63C habitat increased significantly, meanwhile GSH-Px GST activities and GSH content of gills in MH63C habitat were

3 3 - cry1ab/ac cm MH63 (p < 0.01) HH1 CAT d MH63C HH1 MH63C CAT MH HH1 MH63C CAT % 10% (SOD) (CAT) (ACP) -S (GST) (GSH) (GSH-PX) 1.3 SOD [19],CAT [20] [20],ACP SOD CAT ACP U mg -1 progst CDNB [21] 1 SOD GSH GSH-PX Fig. 1 Comparision the activities of SOD of, Cipangopaludina cathayensis in different rice fields [22], mg ml SPSS17.0 (ANOVA) S-N-K LSD 2 Results 2.1 SOD Bt 1 (HH1) Bt 63(MH63) Bt 63(MH63C)3 2 SOD 1 HH1 SOD MH63 HH1 SOD MH63 Fig. 2 Comparision the activities of CAT of MH63C SOD HH1 MH63 (p < 0.01) MH63C MH63 (p < 0.05) HH1 Note: ** ** p < 0. 01, p < 0. 01, Same for below CAT 2.3 GSH-Px GSH-Px HH1 MH63 MH63C SOD MH % MH63C (p >0.05) GSH-Px MH % HH1 MH63C HH1 MH63C MH63C (p > 0.05) HH1 MH63 (p <0. 01) HH1 MH63 SOD SOD GSH-Px (p >0.05)( 3) MH63C HH1 MH63 MH MH63C HH1 MH63C HH1 MH63 GSH GSH 4 HH1 MH63C (p > 0.05) GSH MH CAT (p > 0.05) MH63 MH63C 3 CAT GSH MH63 GSH CAT HH1 (p <0.05) HH1 GSH MH63C MH63C SOD MH63

4 SPSS17.0 (ANOVA) S-N-K LSD 2 Results 2.1 SOD Bt 1 (HH1) Bt 63(MH63) Bt 63(MH63C)3 2 SOD 1 HH1 SOD MH63 HH1 SOD MH63 Fig. 2 Comparision the activities of CAT of MH63C SOD HH1 MH63 (p < 0.

4 520 9 (p > 0.05)( 6) 3 GSH-Px Fig. 3 The activities of GSH-Px of MH63 HH1 (p < 0.01) MH63C MH63 GST 66.16% HH1 MH63 (p > 0.05) GST GST MH63C MH63C GST HH1 MH (p < 0.01) HH1 GST MH63 4 GSH * p < Fig. 4 The GSH content of Note: * p < ACP [23] Cry1Ab/Cry1Ac HH1 ACP Cnaphalocrocis medinalis (Guenée) MH63 MH63C (p < 0.01)HH1 Bt ACP MH63 MH63C SOD CAT POD % 98.8% MH63C MH63 ACP MH63C HH1 MH63 3 ACP Cry1Ab/Cry1Ac 1 (HH1) (p > 0.05)( 5) 5 ACP Fig. 5 The activities of ACP of 2.6 GST 6 GST Fig. 6 The activities of GST of 3 Discussion MH63 HH1 SOD CAT GSH- PX MH63 SOD CAT 3 O - H 2 2 O 2 HH1 Bt O - H 2 2 O 2 Bt Bt Bt Bt Bt MH63C GST

5 ACP [23] Cry1Ab/Cry1Ac HH1 ACP Cnaphalocrocis medinalis (Guenée) MH63 MH63C (p < 0.01)HH1 Bt ACP MH63 MH63C SOD CAT POD 3 112.4% 98.

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