奶制品中三聚氰胺快速检测ELISA试剂盒

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1 Tribo TM Aflatoxin B1 Rapid Test Strip User Guide (Catalog No. TBS11166) TribioScience, Inc.

2 General Description Aflatoxins are a class of structurally similar mycotoxins produced by Aspergillus species, with the most naflatoxin B1ble being Aspergillus flavus. Currently, 17 different types of aflatoxins have been discovered, including aflatoxins B1 and B2 (produced by A. flavus and A. parasiticus), aflatoxins G1 and G2 (produced by A. parasiticus), and aflatoxins M1 and M2, metabolites of B1 and B2 respectively. Aflatoxin B1, the most common form of aflatoxin originated in naturally contaminated food, has acute toxicity to animals and has been classified as a Group 1 carcinogen by IARC of World Health Organization. Tribo TM Aflatoxin B1 Rapid Test Strip provides a rapid and convenient test for aflatoxin B1 with a colloidal gold immunochromatographic design, effectively preventing food contaminated with aflatoxins from being consumed and is an effective tool for food safety. It provides a fast (results shown in 10 minutes), simple (no instrument is required, and results can be observed visually), sensitive and reliable detection approach for the presence of aflatoxin B1 in Produces including corn, rice, beans, peanuts, nut, cereal, etc., as well as peanut butter, animal feed, and cooking oil. The lower limit of detection (LOD) of aflatoxin B1 in the sample is 5 ng/ml (5 ppb). Intended Use Tribo TM Aflatoxin B1 Test Strip is a lateral flow strip test for rapid detection of aflatoxin B1 in grain samples. Assay sensitivity: 5 ng/ml (5 ppb) Safety Instructions To receive complete safety information on this product, contact TribioScience, Inc. and request Material Safety Data Sheet. Assay Principles Tribo TM Aflatoxin B1 Test Strip is based on the principle of colloidal gold immunochromatography. An anti-aflatoxin B1 antibody is conjugated to colloidal gold and placed on conjugate pad. Colloidal gold provides red color to visualize antibody-antigen binding. Aflatoxin B1 antigen is immobilized on nitrocellulose membrane. After test sample is loaded onto sample pad, it mixes with gold-antibody conjugate and migrates together along the membrane. If sample contains no aflatoxin B1, antibody conjugated to colloidal gold will bind the antigen immobilized on membrane, leading to clear red color presented - 2 -

3 on membrane detection line where the test antigen is immobilized (indicating a negative result). If aflatoxin B1 is present in the test sample, it will bind gold-antibody conjugate and prevent its binding onto the antigen line on membrane. As a result, no color will be visible on detection line on membrane (indicating a positive result). Reagents and Materials in each pack a) 1 Aflatoxin B1 Test Strip b) 1 disposable dropper c) 1 pack of desiccant d) 1 bottle of sample diluent Sample Collection and Test Procedure Equilibrate test strip to room temperature (20-25 o C) Equilibrate test sample to room temperature (20-25 o C) a) Pulverize over 5 g of representative grain sample, and pass through a sieve with mesh size of 20. Place 2 grams of this pulverized sample into a 50 ml centrifuge tube. b) Add 8 ml ethyl acetate (EtAc) into this centrifuge tube and close lid tightly. Shake violently (or vortex) for 5 minutes and let stand afterwards. If less than 3 ml supernatant is present after sedimentation, centrifuge at 4,000 rpm for 1 minute. c) Transfer 3 ml supernatant into a clean glass beaker, blow air over surface until solvent evaporates completely. Re-dissolve residue in 200 µl sample diluent (included with the test strip) by shaking and mixing thoroughly. Take 100 µl of this re-dissolved solution and add another 100 µl sample diluent to obtain test solution. d) Add 2 drops of test solution described above into sample loading well with a disposable dropper. e) Observe result in 5-10 minutes. Result Interpretation Test result is interpreted by observing test line and control line shown in result window. Negative (-): both test and control lines are present, indicating Aflatoxin B1 concentration in sample is lower than 5 ng/ml; Positive (+): control line is present, and test line is absent. This result indicates Aflatoxin B1 centration is higher than 5 ng/ml in the sample; Invalid test: no control line is present. Please repeat the test using a new test strip following instructions on this user guide

4 Negative Positive Invalid Sample Loading Sample Loading Sample Loading Test Line Test Line Test Line Control Line Control Line Control Line Precautions 1. Test strip is for one-time use only. Please use the test strip on the same day the package is opened. 2. Do not use tap water, purified water, or distilled water as negative control. 3. Test should be performed at room temperature, and test strip and sample both need to be equilibrated to room temperature before the test. 4. If no liquid movement is observed in the test window 30 seconds after test solution is added to sample loading well, add one more drop of test solution. 5. Positive samples identified by test strip are recommended to be verified with other detection methods (such as HPLC or GC-MS). Storage and Expiration Date Storage: Keep at room temperature in a cool and dry place in intact original packaging. Expiration Date: 12 months after manufacturing date. Technical Assistance For ordering or technical assistance regarding this product, or for additional information about TribioScience products, please info@tribioscience.com or call (650) General Limited Warranty - 4 -

5 TribioScience, Inc. warrants its manufactured products against defects in materials and workmanship when used in accordance with the applicable instructions for a period not to extend beyond a product s printed expiration date. TribioScience makes no other warranty, expressed or implied. There is no warranty of merchantability or fitness for a particular purpose. The warranty provided herein and the data, specifications and descriptions of TribioScience products appearing in published catalogues and product literature may not be altered except by express written agreement signed by an officer of TribioScience. Representations, oral or written, which are inconsistent with this warranty or such publications are not authorized and, if given, should not be relied upon. In the event of a breach of the foregoing warranty, TribioScience Inc. s sole obligation shall be to repair or replace, at its option, any product or part thereof that proves defective in materials or workmanship within the warranty period, provided the customer notifies TribioScience promptly of any such defect. The exclusive remedy provided herein shall not be deemed to have failed of its essential purpose so long as TribioScience is willing and able to repair or replace any nonconforming TribioScience product or part. TribioScience shall not be liable for consequential, incidental, special or any other indirect damages resulting from economic loss or property damage sustained by a customer from the use of its products. However, in some states the purchaser may have rights under state law in addition to those provided by this warranty. For research use only

6 Tribo TM 黄曲霉毒素 B1 快速检测试纸卡使用说明书 ( 产品编号 :TBS11166) 1. 产品概述 : 黄曲霉毒素 (Aflatoxins) 是由生长在食物及饲料中的黄曲霉菌曲霉菌等代谢产生的一组化学结构类似的真菌毒素目前已分离鉴定出 17 种, 主要是黄曲霉毒素 B1 B2 G1 G2, 以及由 B1 和 B2 在体内经过羟化而衍生成的代谢产物 M1 和 M2 等在天然污染的食品中以黄曲霉毒素 B1 最多见, 毒性最强, 已被 WHO 划定为一类致癌物本产品黄曲霉毒素 B1 快速检测试纸卡将胶体金免疫层析原理应用于黄曲霉毒素 B1 含量的快速测定筛选检测, 为防止谷物中黄曲霉毒素 B1 的滋生和保障消费者的食品安全提供高效的检测手段产品适用范围 : 粮食作物 ( 玉米大米豆类花生坚果干果麦类等 ) 花生酱饲料食用油等本产品具有灵敏度高特异性好, 并且操作简便的特性, 不需要任何仪器设备, 可通过肉眼直接判读结果样本中黄曲霉毒素 B1 的最低检出限为 5ng/ml(5ppb) 2. 用途及灵敏度 : 本产品黄曲霉毒素 B1 快速检测试纸卡适用于测定粮食作物 ( 玉米大米豆类花生坚果干果麦类等 ) 花生酱饲料食用油等产品中黄曲霉毒素 B1 的存在检测灵敏度 :5ng/ml(5ppb) 3. 检测原理 : 黄曲霉毒素 B1 快速检测试纸卡将胶体金免疫层析原理应用于黄曲霉毒素 B1 含量的快速测定在试纸卡中, 抗黄曲霉毒素 B1 抗体偶联到胶体金颗粒上, 黄曲霉毒素 B1 测试抗原固定在纤维素滤膜上如果样品不含有黄曲霉毒素 B1, 胶体金抗体偶联物与固定抗原结合, 在膜上显示检测线, 检测结果为阴性如果样品中含有黄曲霉毒素 B1, 黄曲霉毒素 B1 结合在胶体金抗体偶联物上, 抑制了胶体金抗体偶联物与固定抗原的结合, 不显示检测线, 检测结果为阳性 - 6 -

7 4. 试纸卡产品组成 : 试纸卡 (1 块 ); 一次性吸管 (1 支 ); 干燥剂 (1 块 ); 样品稀释液 (1 瓶 ) 5. 检测步骤 : (1) 取 5g 以上有代表性的样品粉碎 ( 过 20 目筛 ), 称取 2g 均匀粉碎试样加入到离心管中 (2) 离心管中准确加入 8ml 乙酸乙酯, 将瓶塞盖紧密封, 用力振荡 5 分钟 ( 如上清不足 3ml, 以 4000rpm 离心 1 分钟 ) (3) 用吸管取 3mL 上清液到小玻璃杯中, 吹干滤液, 用 200μl 稀释液复溶, 振荡混匀 ; 取 100 μl 复溶液再加入 100μl 稀释液混匀, 待测 (4) 用一次性吸管吸取待检样品, 在加样孔内滴加样品 2 滴 (5) 加样后计时,5-10 分钟后判定结果 6. 结果判定 : 阴性 (-): 检测线 (T) 和控制线 (C) 都显色,T 线深度等于或深于 C 线表明样品中黄曲霉毒素 B1 的浓度低于 5ppb 阳性 (+): 检测线 (T) 无色, 控制线 (C) 显色,T 线不显色或者浅于 C 线深度表明样品中黄曲霉毒素 B1 的浓度高于 5ppb 无效 : 控制线 (C) 无色, 表示使用方法不正确或试纸卡失效, 结果无效请对照使用说明书, 用新的试纸卡重新测定阴性阳性检测无效加样孔加样孔加样孔 T 检测线 T 检测线 T 检测线 C 控制线 C 控制线 C 控制线 7. 注意事项 : (1) 黄曲霉毒素 B1 快速检测试纸卡为一次性使用打开包装后, 请在当日使用 (2) 请勿使用自来水纯化水及蒸馏水作为阴性对照 (3) 在室温 (20-25 o C) 下使用, 使用前将检测卡和待检样本恢复至室温 (20-25 o C) 测试 - 7 -

8 (4) 如滴加检测液后 30 秒内, 在测试窗口无液体移行, 则再补加 1 滴检测液 (5) 本品检测为阳性的样本, 建议采用更精确的方法 ( 如 HPLC 或 GC-MS) 加以复核 8. 储存条件和有效期 : 黄曲霉毒素 B1 快速检测试纸卡原包装在室温 (20-25 o C) 下储存, 置于阴凉避光干燥处有效期为 12 个月, 产品批号及有效期见包装袋本产品仅供研究使用 - 8 -

奶制品中三聚氰胺快速检测ELISA试剂盒

奶制品中三聚氰胺快速检测ELISA试剂盒 Tribo TM Ochratoxin A Rapid Test Strip User Guide (Catalog No. TBS11161) TribioScience, Inc. General Description Ochratoxin A (OTA) is a toxic secondary metabolite produced by some Aspergillus and Penicillium